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The map label for this gene is spoT [H]

Identifier: 74316489

GI number: 74316489

Start: 493680

End: 495896

Strand: Reverse

Name: spoT [H]

Synonym: Tbd_0471

Alternate gene names: 74316489

Gene position: 495896-493680 (Counterclockwise)

Preceding gene: 74316490

Following gene: 74316488

Centisome position: 17.04

GC content: 66.26

Gene sequence:

>2217_bases
GTGAAATCCTCAACCGGGGCCGCGCCTGAAAACTCGCGCACCCGCAAGCCGGCGCCCGCCCCGCGTGCGCCGGCAATGAC
GCACGGCTTCGACACACTGCGCGAGCATCTGGCCCACCTCGGGCCGGAAGATGTCGCTCAGCTCGAACAGGCCTACGAAT
TCAGCCGCAGCGCCCATGAAGGGCAGTTTCGCAAGAGCGGCGAACCCTATATCTCCCATCCGCTCGCCGTCGCCGCGATC
CTCGCGGACTGGCATCTCGACGCCCAGACCCTCTGCGCCGCCCTGCTGCACGACGTCGTCGAGGACACGCCGGCGACGGC
CGGCGAGATCGAAGCCCGCTTCGGCAAGGCCGTCGCGGTGCTGGTCGGCGGCGTCTCCAAGCTCGACAAGCTCGCCTTCG
AATCCGAGCAGCACGCGCAGGCGGAAAACTTCCGCAAGATGCTGCTGGCGATGGCGCAGGACGTCCGCGTGATCCTGGTC
AAGCTCGCCGATCGCCTGCACAACATGCACACGATGGGCGCGATGCCCGCCGAAAAGCGCCGCCGCATCGCGACCGAAAC
GCTGGAGATCTACGCGCCGATCGCCAACCGGCTCGGCCTGCACTCGGTCTACCAGGAACTCGAGGATCTCGGCTTCCGCT
ACAGCCATCCCAACCGCTACCAGGTGCTCGCCAAGGCGGTGAAGGCCGCGCGGGGCAACCGTCGCGAGCTGGTCGAACGG
GTCAAGGTCGCGCTGCAGGAAAAGCTCGAGCAATGCAAGATCGAGGCCGCGATCAGCGGCCGCGAAAAGCACCTCTACAG
CATCTACCGCAAGATGCAGGAGAAAAACCTCGCGTTTTCCGAGGTCTTCGACATCTACGGCTTTCGCGTCGTCGTGCGGG
ACCAGCCCGCCTGCTACCTCGCGCTCGGCGCGCTGCATGCGCTCTACAAGCCGATTCCGGGCAAGTTCAAGGACTACATC
GCGATCCCCAAGGCCAACGGCTACCAGTCGCTGCATTCGATCCTGTTCGGCCCCAACGGCACGCCGATCGAAGTCCAGAT
CCGCACCGTCGACATGAACCGTCTCGCCGAGTCCGGCGTCGCGTCGCACTGGATGTACAAATCGGCCGACACCGCGCTGA
ACGACGTGCAGACGCGCACCCACCGCTGGCTGCAGTCGCTGCTCGACATCCAGGCCGACCACGGCGACTCGCCCGAATTC
CTCGAACATATCAAAGTCGACCTCTTCCCCGACGAGGTCTACGTGTTCACGCCGAAGGGCAAGATCATGGCCTTGCCGCG
CGGGGCGACCGCGGTCGACTTCGCCTTCGCCGTGCACAGCGACGTCGGCCACCGCTGCATCGCGGTCAAGATCAACTACG
AGCTGATGCCGCTGCGCACGCAGCTCAGGAACGGCGATCACGTCGAGGTGCTGACCGCGTCGAACGCGAGCCCCAACGCC
GCCTGGCTCAACTTCGTCGTCACCGGCAAGGCGCGCTCGCACATCCGCAACTACCTGCGCAACACGCGGGTCGAGGAAGC
CGCGGCGCTCGGCGAACGCCTGCTCAGGAACGCGATCGAGGCGCTGCATCCGGGCGCCGAAGCCGACCCCACGGTGTGGG
ATCGCGTCGTCAAGGACTACGGCCTGCAGACCCGCGACGAACTCTTCGCCGGGATCGGCCTCGGCCGCAAGCTGCCGCTC
GTCGTCGCCCACCAGCTGCTGCACGTTCAGGGCGAGAAGACCGGCGAGCCCGCGCATCCGCACGCGATCAGCATCCGCGG
CACCGAAGGCATCGCGGTCGAACTGGCGCAGTGCTGCCATCCGATTCCGGGTGACCCGATTCTCGGCTTCATCCACAAGG
ACCGCGGCCTCATCATCCACACCCACGACTGCCCGTCGATCCGCGCCTTCCGCACCGATCCCGAGAAATGGCTCGACGTC
GAATGGGAGGCCGAGCCGGGCCGCATGTTCGACGTCTCGATCAAGGTCGTCGTCGGCAACAAGCGCGGCGTTCTGGCCAA
GGTCGCCGCCTCGATCGCCGAACACGGCTCGAACATCGGCAACGTGTCGATGGAGGAAGAGGACGGCAGCCCGTACACCG
TGCTGTTCTTCACGGTCCAGGTCGAAAATCGCGCCCACCTCGCGCGCGTCATGCGCGGCCTGCGCATGCTGCCCGACGTC
GTCCGGATCTTCCGCATCAAGGGCAAGAAAGACGGCCGCGCGGCCCCCCTCCAATAA

Upstream 100 bases:

>100_bases
CGCGCCCGCCAGCTGGCCCAGGGCTCGCAGCCCATGGTCGAATCGAAGGACAAACCCATCGTCACCGCGCTGCGCGAGAT
CGCGGCAGGCAAGGTCGGTC

Downstream 100 bases:

>100_bases
ATTCTTCTGCTTCAGGATCCAGCATGGACAAGCGCATCATCCAGACCGCCGACGCCCCCGCCGCGATCGGTACCTACTCG
CAGGCAATCCGCGTGGACAA

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MKSSTGAAPENSRTRKPAPAPRAPAMTHGFDTLREHLAHLGPEDVAQLEQAYEFSRSAHEGQFRKSGEPYISHPLAVAAI
LADWHLDAQTLCAALLHDVVEDTPATAGEIEARFGKAVAVLVGGVSKLDKLAFESEQHAQAENFRKMLLAMAQDVRVILV
KLADRLHNMHTMGAMPAEKRRRIATETLEIYAPIANRLGLHSVYQELEDLGFRYSHPNRYQVLAKAVKAARGNRRELVER
VKVALQEKLEQCKIEAAISGREKHLYSIYRKMQEKNLAFSEVFDIYGFRVVVRDQPACYLALGALHALYKPIPGKFKDYI
AIPKANGYQSLHSILFGPNGTPIEVQIRTVDMNRLAESGVASHWMYKSADTALNDVQTRTHRWLQSLLDIQADHGDSPEF
LEHIKVDLFPDEVYVFTPKGKIMALPRGATAVDFAFAVHSDVGHRCIAVKINYELMPLRTQLRNGDHVEVLTASNASPNA
AWLNFVVTGKARSHIRNYLRNTRVEEAAALGERLLRNAIEALHPGAEADPTVWDRVVKDYGLQTRDELFAGIGLGRKLPL
VVAHQLLHVQGEKTGEPAHPHAISIRGTEGIAVELAQCCHPIPGDPILGFIHKDRGLIIHTHDCPSIRAFRTDPEKWLDV
EWEAEPGRMFDVSIKVVVGNKRGVLAKVAASIAEHGSNIGNVSMEEEDGSPYTVLFFTVQVENRAHLARVMRGLRMLPDV
VRIFRIKGKKDGRAAPLQ

Sequences:

>Translated_738_residues
MKSSTGAAPENSRTRKPAPAPRAPAMTHGFDTLREHLAHLGPEDVAQLEQAYEFSRSAHEGQFRKSGEPYISHPLAVAAI
LADWHLDAQTLCAALLHDVVEDTPATAGEIEARFGKAVAVLVGGVSKLDKLAFESEQHAQAENFRKMLLAMAQDVRVILV
KLADRLHNMHTMGAMPAEKRRRIATETLEIYAPIANRLGLHSVYQELEDLGFRYSHPNRYQVLAKAVKAARGNRRELVER
VKVALQEKLEQCKIEAAISGREKHLYSIYRKMQEKNLAFSEVFDIYGFRVVVRDQPACYLALGALHALYKPIPGKFKDYI
AIPKANGYQSLHSILFGPNGTPIEVQIRTVDMNRLAESGVASHWMYKSADTALNDVQTRTHRWLQSLLDIQADHGDSPEF
LEHIKVDLFPDEVYVFTPKGKIMALPRGATAVDFAFAVHSDVGHRCIAVKINYELMPLRTQLRNGDHVEVLTASNASPNA
AWLNFVVTGKARSHIRNYLRNTRVEEAAALGERLLRNAIEALHPGAEADPTVWDRVVKDYGLQTRDELFAGIGLGRKLPL
VVAHQLLHVQGEKTGEPAHPHAISIRGTEGIAVELAQCCHPIPGDPILGFIHKDRGLIIHTHDCPSIRAFRTDPEKWLDV
EWEAEPGRMFDVSIKVVVGNKRGVLAKVAASIAEHGSNIGNVSMEEEDGSPYTVLFFTVQVENRAHLARVMRGLRMLPDV
VRIFRIKGKKDGRAAPLQ
>Mature_738_residues
MKSSTGAAPENSRTRKPAPAPRAPAMTHGFDTLREHLAHLGPEDVAQLEQAYEFSRSAHEGQFRKSGEPYISHPLAVAAI
LADWHLDAQTLCAALLHDVVEDTPATAGEIEARFGKAVAVLVGGVSKLDKLAFESEQHAQAENFRKMLLAMAQDVRVILV
KLADRLHNMHTMGAMPAEKRRRIATETLEIYAPIANRLGLHSVYQELEDLGFRYSHPNRYQVLAKAVKAARGNRRELVER
VKVALQEKLEQCKIEAAISGREKHLYSIYRKMQEKNLAFSEVFDIYGFRVVVRDQPACYLALGALHALYKPIPGKFKDYI
AIPKANGYQSLHSILFGPNGTPIEVQIRTVDMNRLAESGVASHWMYKSADTALNDVQTRTHRWLQSLLDIQADHGDSPEF
LEHIKVDLFPDEVYVFTPKGKIMALPRGATAVDFAFAVHSDVGHRCIAVKINYELMPLRTQLRNGDHVEVLTASNASPNA
AWLNFVVTGKARSHIRNYLRNTRVEEAAALGERLLRNAIEALHPGAEADPTVWDRVVKDYGLQTRDELFAGIGLGRKLPL
VVAHQLLHVQGEKTGEPAHPHAISIRGTEGIAVELAQCCHPIPGDPILGFIHKDRGLIIHTHDCPSIRAFRTDPEKWLDV
EWEAEPGRMFDVSIKVVVGNKRGVLAKVAASIAEHGSNIGNVSMEEEDGSPYTVLFFTVQVENRAHLARVMRGLRMLPDV
VRIFRIKGKKDGRAAPLQ

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=140, Percent_Identity=37.8571428571429, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790082, Length=700, Percent_Identity=50.8571428571429, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=684, Percent_Identity=34.5029239766082, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25143118, Length=126, Percent_Identity=37.3015873015873, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 82092; Mature: 82092

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSTGAAPENSRTRKPAPAPRAPAMTHGFDTLREHLAHLGPEDVAQLEQAYEFSRSAHE
CCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
GQFRKSGEPYISHPLAVAAILADWHLDAQTLCAALLHDVVEDTPATAGEIEARFGKAVAV
CCHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LVGGVSKLDKLAFESEQHAQAENFRKMLLAMAQDVRVILVKLADRLHNMHTMGAMPAEKR
HHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRIATETLEIYAPIANRLGLHSVYQELEDLGFRYSHPNRYQVLAKAVKAARGNRRELVER
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
VKVALQEKLEQCKIEAAISGREKHLYSIYRKMQEKNLAFSEVFDIYGFRVVVRDQPACYL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCHHH
ALGALHALYKPIPGKFKDYIAIPKANGYQSLHSILFGPNGTPIEVQIRTVDMNRLAESGV
HHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEECHHHHHHCCH
ASHWMYKSADTALNDVQTRTHRWLQSLLDIQADHGDSPEFLEHIKVDLFPDEVYVFTPKG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEECCCEEEECCCC
KIMALPRGATAVDFAFAVHSDVGHRCIAVKINYELMPLRTQLRNGDHVEVLTASNASPNA
CEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCEEEEHHHHHCCCCEEEEEEECCCCCCC
AWLNFVVTGKARSHIRNYLRNTRVEEAAALGERLLRNAIEALHPGAEADPTVWDRVVKDY
EEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
GLQTRDELFAGIGLGRKLPLVVAHQLLHVQGEKTGEPAHPHAISIRGTEGIAVELAQCCH
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHCC
PIPGDPILGFIHKDRGLIIHTHDCPSIRAFRTDPEKWLDVEWEAEPGRMFDVSIKVVVGN
CCCCCCEEEEEECCCCEEEEECCCCCCEEECCCHHHHEEEEEECCCCEEEEEEEEEEECC
KRGVLAKVAASIAEHGSNIGNVSMEEEDGSPYTVLFFTVQVENRAHLARVMRGLRMLPDV
CCCHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHH
VRIFRIKGKKDGRAAPLQ
HHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKSSTGAAPENSRTRKPAPAPRAPAMTHGFDTLREHLAHLGPEDVAQLEQAYEFSRSAHE
CCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
GQFRKSGEPYISHPLAVAAILADWHLDAQTLCAALLHDVVEDTPATAGEIEARFGKAVAV
CCHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LVGGVSKLDKLAFESEQHAQAENFRKMLLAMAQDVRVILVKLADRLHNMHTMGAMPAEKR
HHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RRIATETLEIYAPIANRLGLHSVYQELEDLGFRYSHPNRYQVLAKAVKAARGNRRELVER
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
VKVALQEKLEQCKIEAAISGREKHLYSIYRKMQEKNLAFSEVFDIYGFRVVVRDQPACYL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCHHH
ALGALHALYKPIPGKFKDYIAIPKANGYQSLHSILFGPNGTPIEVQIRTVDMNRLAESGV
HHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEECHHHHHHCCH
ASHWMYKSADTALNDVQTRTHRWLQSLLDIQADHGDSPEFLEHIKVDLFPDEVYVFTPKG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEECCCEEEECCCC
KIMALPRGATAVDFAFAVHSDVGHRCIAVKINYELMPLRTQLRNGDHVEVLTASNASPNA
CEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCEEEEHHHHHCCCCEEEEEEECCCCCCC
AWLNFVVTGKARSHIRNYLRNTRVEEAAALGERLLRNAIEALHPGAEADPTVWDRVVKDY
EEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
GLQTRDELFAGIGLGRKLPLVVAHQLLHVQGEKTGEPAHPHAISIRGTEGIAVELAQCCH
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHCC
PIPGDPILGFIHKDRGLIIHTHDCPSIRAFRTDPEKWLDVEWEAEPGRMFDVSIKVVVGN
CCCCCCEEEEEECCCCEEEEECCCCCCEEECCCHHHHEEEEEECCCCEEEEEEEEEEECC
KRGVLAKVAASIAEHGSNIGNVSMEEEDGSPYTVLFFTVQVENRAHLARVMRGLRMLPDV
CCCHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHH
VRIFRIKGKKDGRAAPLQ
HHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]