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The map label for this gene is ispE

Identifier: 74316404

GI number: 74316404

Start: 414539

End: 415375

Strand: Direct

Name: ispE

Synonym: Tbd_0386

Alternate gene names: 74316404

Gene position: 414539-415375 (Clockwise)

Preceding gene: 74316403

Following gene: 74316405

Centisome position: 14.25

GC content: 67.98

Gene sequence:

>837_bases
ATGAGCCAGGCGTTTCCCGCGCCGGCGAAGCTCAATCTTTTCCTCCACGTCGTCGGTCGCCGCGACGACGGATACCATCT
GCTGCAGAGCGTGTTTCGTCTGATCGACCGCGCCGACACCGTGCACCTGGAATTGCGCGATGACGGCCGAATCGTGCGCG
AAGGCGCTTTGCCGGGCGTGAGCGAAGATCAGGATCTGACCGTCCGCGCGGCCCGACTCCTGCAGCCCTACGCGCGCCCG
GGCGCCGGGGTCGGCATCCGTCTCGACAAGCGGCTGCCGATGGGCGGGGGCCTCGGCGGCGGCAGTTCCGATGCGGCGAC
CGTGCTGCTCGCGCTCAACCGCCTGTGGGAGGTCGATCTGCCGCGCCAGCGCTTGCAGGCGCTCGCCCTGCGACTCGGCG
CCGACGTGCCGGTCTTCGTTTTCGGTCAGACGGCGTTCGCGGAGGGGGTCGGCGAACTGTTGCAGCCGATCGGCGCGCCG
GTCGCGTGGTACGTCGTGCTGACGCCGCCGGTGCACGTGCCGACCGCGGCAATTTTTGCCGCGCCGGAATTGACACGCAA
CACGCCTGCCCTCAAAATAGCGCCCTTTTCCGCGGGCATGGGTCATAACGACCTCGAGCCCGTGGTCGTCGGCCGGTACC
CGGAAGTCGGTCGCCACCTGCAGTGGCTCGGTCAGTTCGGGGAGGCGCGGATGACCGGTTCGGGCGCCTGCGTTTTCGCA
TCGTTCGCAACCGAGGATGCCGCGCGCAGCGTGCTGCAGGCGCTGCCCGATACGATGCAGGGTTTTGTGGCGCGAGGTCT
GGACAAGCACCCCTTGTACGACTTTGTGCCTGAGTAG

Upstream 100 bases:

>100_bases
ACTATCTGCAATACGGCGCCGACGCCCGGCCTCGCAAGCTCGTCGTGAGCCGTGCGGGCCTCGAAATCCGTCTGGTCGCC
GATAGCTGGCAGTCGGCGCC

Downstream 100 bases:

>100_bases
TAGCCATTGGGGAGTCGCCAAGTGGTAAGGCATCGGATTTTGATTCCGACATTCGTAGGTTCGATCCCTACCTCCCCAGC
CAGATAGAGCGAACAGCCGC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSQAFPAPAKLNLFLHVVGRRDDGYHLLQSVFRLIDRADTVHLELRDDGRIVREGALPGVSEDQDLTVRAARLLQPYARP
GAGVGIRLDKRLPMGGGLGGGSSDAATVLLALNRLWEVDLPRQRLQALALRLGADVPVFVFGQTAFAEGVGELLQPIGAP
VAWYVVLTPPVHVPTAAIFAAPELTRNTPALKIAPFSAGMGHNDLEPVVVGRYPEVGRHLQWLGQFGEARMTGSGACVFA
SFATEDAARSVLQALPDTMQGFVARGLDKHPLYDFVPE

Sequences:

>Translated_278_residues
MSQAFPAPAKLNLFLHVVGRRDDGYHLLQSVFRLIDRADTVHLELRDDGRIVREGALPGVSEDQDLTVRAARLLQPYARP
GAGVGIRLDKRLPMGGGLGGGSSDAATVLLALNRLWEVDLPRQRLQALALRLGADVPVFVFGQTAFAEGVGELLQPIGAP
VAWYVVLTPPVHVPTAAIFAAPELTRNTPALKIAPFSAGMGHNDLEPVVVGRYPEVGRHLQWLGQFGEARMTGSGACVFA
SFATEDAARSVLQALPDTMQGFVARGLDKHPLYDFVPE
>Mature_277_residues
SQAFPAPAKLNLFLHVVGRRDDGYHLLQSVFRLIDRADTVHLELRDDGRIVREGALPGVSEDQDLTVRAARLLQPYARPG
AGVGIRLDKRLPMGGGLGGGSSDAATVLLALNRLWEVDLPRQRLQALALRLGADVPVFVFGQTAFAEGVGELLQPIGAPV
AWYVVLTPPVHVPTAAIFAAPELTRNTPALKIAPFSAGMGHNDLEPVVVGRYPEVGRHLQWLGQFGEARMTGSGACVFAS
FATEDAARSVLQALPDTMQGFVARGLDKHPLYDFVPE

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=279, Percent_Identity=50.8960573476703, Blast_Score=257, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_THIDA (Q3SLR6)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314144.1
- ProteinModelPortal:   Q3SLR6
- SMR:   Q3SLR6
- STRING:   Q3SLR6
- GeneID:   3671648
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0386
- NMPDR:   fig|292415.3.peg.780
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- PhylomeDB:   Q3SLR6
- BioCyc:   TDEN292415:TBD_0386-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29910; Mature: 29779

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAFPAPAKLNLFLHVVGRRDDGYHLLQSVFRLIDRADTVHLELRDDGRIVREGALPGV
CCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCCC
SEDQDLTVRAARLLQPYARPGAGVGIRLDKRLPMGGGLGGGSSDAATVLLALNRLWEVDL
CCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
PRQRLQALALRLGADVPVFVFGQTAFAEGVGELLQPIGAPVAWYVVLTPPVHVPTAAIFA
CHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHC
APELTRNTPALKIAPFSAGMGHNDLEPVVVGRYPEVGRHLQWLGQFGEARMTGSGACVFA
CCHHCCCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEECCCCCEEEE
SFATEDAARSVLQALPDTMQGFVARGLDKHPLYDFVPE
EHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQAFPAPAKLNLFLHVVGRRDDGYHLLQSVFRLIDRADTVHLELRDDGRIVREGALPGV
CCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCCC
SEDQDLTVRAARLLQPYARPGAGVGIRLDKRLPMGGGLGGGSSDAATVLLALNRLWEVDL
CCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
PRQRLQALALRLGADVPVFVFGQTAFAEGVGELLQPIGAPVAWYVVLTPPVHVPTAAIFA
CHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHC
APELTRNTPALKIAPFSAGMGHNDLEPVVVGRYPEVGRHLQWLGQFGEARMTGSGACVFA
CCHHCCCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEECCCCCEEEE
SFATEDAARSVLQALPDTMQGFVARGLDKHPLYDFVPE
EHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA