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The map label for this gene is pqqL [C]

Identifier: 74316395

GI number: 74316395

Start: 405178

End: 406539

Strand: Direct

Name: pqqL [C]

Synonym: Tbd_0377

Alternate gene names: 74316395

Gene position: 405178-406539 (Clockwise)

Preceding gene: 74316391

Following gene: 74316396

Centisome position: 13.92

GC content: 66.74

Gene sequence:

>1362_bases
ATGCATAAGTTATGGGCATTCTGCCTGGCGTTGCTGGCAGGCTCGACACACGCGGCGGCATTGACCGACGTCACGCTGGA
CAATGGGATGCGCGTCATCGTCCACGAGGATCATCGCGCGCCCGTCATGGTTTCGCAGGTGTGGTACCGCGCCGGCGCGG
TGGACGAATTCAATGGCACGACCGGCGTTGCCCACGTGCTCGAACACATGATGTTCAAAGGCACGCCCACGGTGCCGCCC
GGCGAATTCTCGAAGCGGATCGCGGCTGCGGGCGGGCGCGAGAACGCGTTCACGAGCCGCGACTATACCGCCTACTTCCA
GCAGATGCAGAAGGATCGGCTGGCGTTGTCGATGGAGCTCGAGGCCGACCGCATGGCCAACCTCGTGATCAGCGACGAAC
TGTTCGGCAAGGAGCTGCAGGTCGTGATGGAAGAGCGCCGGCTTCGCACCGAGGACCAGCCGCAGGCCGTGGTCTACGAG
CGGCTGATGGCGACCGCCTATCAGGCGCATCCCTACCGCCGCCCTATCATCGGCTGGATGAGCGACCTCCAGAGCATGAC
GGCGGCTGACGCGCGCGACTGGTACGCGCGCTGGTACGCGCCGAACAACGCCACCCTCGTGGTCGCCGGCGACGTCGACC
CTGACGAGGTGGTCGCGCTGGCGAAACGCCATTTCGGCGCCTTGCCCGCACGCGCGCTTCCCGAGCGCAAGCCGCAGGGC
GAGCCCGAGCAGGTCGGCATGAAGCGCATCGTCGTGAAGGCACCGGCGCAGCTGCCTTACCTGCTGATGGCCTGGCACGC
GCCGACGCTCAAGGATTGGGAGCGTGACACCACGCCCTACGCGCTGCAGATTCTTGCCGGCGTTCTGTCCGGCAACGACT
CGGCGCGACTGCAGAAATCACTCGTCAAGACGCAGCAGCTCGCGGTCAACGCCAGTGCGGGATACGACATGGTCGCGCGC
GGCCCGGGACTCTTCATGATCGACGCGACACCCGCACCCGGCAAGTCGGTCGCCGCGCTGGAAAAGGCGATCCGCGCCGA
ACTGCTGCGCATCCAGACCAAGGGCATCAGCGACGCCGAGTTGCAGCGCGTCAAGGCGCAGGTCATCGCCGCCGACGTCT
ACCAGCGCGACTCGCTTTTCTACCAGGCGATGCAGCTGGGCGAATACGTGAGCACGGGCCTGCCGCCCGAGGCCCTGCTG
CGGCGCGTCGAGAAGCTGCGCGCGGTCAGCGCCGAGGACGTCCAGCGCGCGGCGCAGGAGTGGTTGCGCGAAGACCGCCT
GAGCGTGGCCGAACTCGACCCCCAGGCGCTCAAACCGCAGCCTGAACGCACGGCCGTGCCGGGAGTACGTCATGTCAATT
AA

Upstream 100 bases:

>100_bases
GTTTGACGGACTGCGGTATCTTATCATTCGTGCACGCGGCTCCGCTTCGATGCGCGAAGTTCATGAGCGCCGTTCCGGCT
CATCCATCAGAGAGGAAGAC

Downstream 100 bases:

>100_bases
GCGTTTCTTCGCCGTTCTCGTGCTGATCCTGGCGGCGCCGGTGCAGGCCGGGGTCGACATCCAGCATTGGCAGACCCCCG
AGGGCGCGCGGGTCTTCTTC

Product: insulinase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MHKLWAFCLALLAGSTHAAALTDVTLDNGMRVIVHEDHRAPVMVSQVWYRAGAVDEFNGTTGVAHVLEHMMFKGTPTVPP
GEFSKRIAAAGGRENAFTSRDYTAYFQQMQKDRLALSMELEADRMANLVISDELFGKELQVVMEERRLRTEDQPQAVVYE
RLMATAYQAHPYRRPIIGWMSDLQSMTAADARDWYARWYAPNNATLVVAGDVDPDEVVALAKRHFGALPARALPERKPQG
EPEQVGMKRIVVKAPAQLPYLLMAWHAPTLKDWERDTTPYALQILAGVLSGNDSARLQKSLVKTQQLAVNASAGYDMVAR
GPGLFMIDATPAPGKSVAALEKAIRAELLRIQTKGISDAELQRVKAQVIAADVYQRDSLFYQAMQLGEYVSTGLPPEALL
RRVEKLRAVSAEDVQRAAQEWLREDRLSVAELDPQALKPQPERTAVPGVRHVN

Sequences:

>Translated_453_residues
MHKLWAFCLALLAGSTHAAALTDVTLDNGMRVIVHEDHRAPVMVSQVWYRAGAVDEFNGTTGVAHVLEHMMFKGTPTVPP
GEFSKRIAAAGGRENAFTSRDYTAYFQQMQKDRLALSMELEADRMANLVISDELFGKELQVVMEERRLRTEDQPQAVVYE
RLMATAYQAHPYRRPIIGWMSDLQSMTAADARDWYARWYAPNNATLVVAGDVDPDEVVALAKRHFGALPARALPERKPQG
EPEQVGMKRIVVKAPAQLPYLLMAWHAPTLKDWERDTTPYALQILAGVLSGNDSARLQKSLVKTQQLAVNASAGYDMVAR
GPGLFMIDATPAPGKSVAALEKAIRAELLRIQTKGISDAELQRVKAQVIAADVYQRDSLFYQAMQLGEYVSTGLPPEALL
RRVEKLRAVSAEDVQRAAQEWLREDRLSVAELDPQALKPQPERTAVPGVRHVN
>Mature_453_residues
MHKLWAFCLALLAGSTHAAALTDVTLDNGMRVIVHEDHRAPVMVSQVWYRAGAVDEFNGTTGVAHVLEHMMFKGTPTVPP
GEFSKRIAAAGGRENAFTSRDYTAYFQQMQKDRLALSMELEADRMANLVISDELFGKELQVVMEERRLRTEDQPQAVVYE
RLMATAYQAHPYRRPIIGWMSDLQSMTAADARDWYARWYAPNNATLVVAGDVDPDEVVALAKRHFGALPARALPERKPQG
EPEQVGMKRIVVKAPAQLPYLLMAWHAPTLKDWERDTTPYALQILAGVLSGNDSARLQKSLVKTQQLAVNASAGYDMVAR
GPGLFMIDATPAPGKSVAALEKAIRAELLRIQTKGISDAELQRVKAQVIAADVYQRDSLFYQAMQLGEYVSTGLPPEALL
RRVEKLRAVSAEDVQRAAQEWLREDRLSVAELDPQALKPQPERTAVPGVRHVN

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Homo sapiens, GI46593007, Length=226, Percent_Identity=30.0884955752212, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI94538354, Length=203, Percent_Identity=33.0049261083744, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1787770, Length=222, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71999683, Length=204, Percent_Identity=28.921568627451, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17553678, Length=217, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323192, Length=438, Percent_Identity=24.2009132420091, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323421, Length=316, Percent_Identity=22.4683544303797, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357875, Length=436, Percent_Identity=26.1467889908257, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24646943, Length=436, Percent_Identity=26.1467889908257, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR001431
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 50345; Mature: 50345

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00143 INSULINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKLWAFCLALLAGSTHAAALTDVTLDNGMRVIVHEDHRAPVMVSQVWYRAGAVDEFNGT
CCHHHHHHHHHHHCCCCHHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCC
TGVAHVLEHMMFKGTPTVPPGEFSKRIAAAGGRENAFTSRDYTAYFQQMQKDRLALSMEL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEE
EADRMANLVISDELFGKELQVVMEERRLRTEDQPQAVVYERLMATAYQAHPYRRPIIGWM
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
SDLQSMTAADARDWYARWYAPNNATLVVAGDVDPDEVVALAKRHFGALPARALPERKPQG
HHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHCCCCCCCC
EPEQVGMKRIVVKAPAQLPYLLMAWHAPTLKDWERDTTPYALQILAGVLSGNDSARLQKS
CHHHHHHHHHEEECCCCCCEEHEEECCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LVKTQQLAVNASAGYDMVARGPGLFMIDATPAPGKSVAALEKAIRAELLRIQTKGISDAE
HHHHHHHEEECCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
LQRVKAQVIAADVYQRDSLFYQAMQLGEYVSTGLPPEALLRRVEKLRAVSAEDVQRAAQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
WLREDRLSVAELDPQALKPQPERTAVPGVRHVN
HHHHHCCCHHHCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHKLWAFCLALLAGSTHAAALTDVTLDNGMRVIVHEDHRAPVMVSQVWYRAGAVDEFNGT
CCHHHHHHHHHHHCCCCHHHEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCC
TGVAHVLEHMMFKGTPTVPPGEFSKRIAAAGGRENAFTSRDYTAYFQQMQKDRLALSMEL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEE
EADRMANLVISDELFGKELQVVMEERRLRTEDQPQAVVYERLMATAYQAHPYRRPIIGWM
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
SDLQSMTAADARDWYARWYAPNNATLVVAGDVDPDEVVALAKRHFGALPARALPERKPQG
HHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHCCCCCCCC
EPEQVGMKRIVVKAPAQLPYLLMAWHAPTLKDWERDTTPYALQILAGVLSGNDSARLQKS
CHHHHHHHHHEEECCCCCCEEHEEECCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LVKTQQLAVNASAGYDMVARGPGLFMIDATPAPGKSVAALEKAIRAELLRIQTKGISDAE
HHHHHHHEEECCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
LQRVKAQVIAADVYQRDSLFYQAMQLGEYVSTGLPPEALLRRVEKLRAVSAEDVQRAAQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
WLREDRLSVAELDPQALKPQPERTAVPGVRHVN
HHHHHCCCHHHCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]