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The map label for this gene is yhcR [H]

Identifier: 74316374

GI number: 74316374

Start: 385696

End: 387663

Strand: Direct

Name: yhcR [H]

Synonym: Tbd_0356

Alternate gene names: 74316374

Gene position: 385696-387663 (Clockwise)

Preceding gene: 74316373

Following gene: 74316375

Centisome position: 13.26

GC content: 65.19

Gene sequence:

>1968_bases
ATGATGCATCGGCTCCACCCCCTCGCCGTCGGCGTGCTGTTGTCGCTTTCCGTCCAAGCCCAGGCCGCCTGTCTGGAACC
CAGCGTTGTGATCGGCACCGTCGATTCCGCCGTGGCCAATAAGGAAATCGGCGCGGCCTGCATGAACGACCTCATCATCG
ATTCCGCTTCCGAAGGCGCCAACTGGGGCAGCCATGGCGAATTCGTGAGCGCGGTGTCGCGGCTCACGCAGGACTGGCTG
AAGGCGGAGTCCATCAGCGCCACCGAGCGCAGCCGGATCGTGAGCGCCGCGGCCCGCTCGGACGTCGGTTCGACGCTGCT
CGTCAAGATTGCGGCGATCAACGATTTCCATGGCCAGCTGTTCAGCCCGGGAAGTTTTGCCGGCAAGCCGGTGGGCGGCA
TCGACGCCCTGGCGGCTTATATCGACGCCATCCGCGCCAAATCGCCCAACCACGTAACGGTTTCCGCGGGCGACCTGATC
GGCGCGACGCCGCTGGTTTCGGCGCTGTTCCACGACGAGCCGACGATCGAGGGCATGAACATCCTGGGCCTGGACTTCAA
CGCCGTCGGCAACCACGAGTTCGACGAGGGGCGCGACGAGCTGCTGCGCATGCAGAACGGCGGCTGCCATCCGACCGACC
CCAACTCGTGCCAAGGCGATGCAGTCGGCACGCCGGTCCCGTTCGAGGGCGCCCGCTTCAAATTCCTGGCGGCCAACGTC
ACCGAAAAGACCGACGGCAAGACGCTGTTCCCTGCGTACGCGATCCGAAATTTCAAGGGCAACAAGGTCGCCTTCATCGG
CATGACGCTCGAGGACACGCCCAGCATCGTCACGCCCAGCGGCATTTCGACGCTGGATTTCCACGACGAGGCCGACACGG
TCAACGCGCTGATCCCCGAACTGAAGGCGCAGGGCGTGGAAAGCATCGTGGTGCTAGTCCACGAAGGCGGCGTGGATACC
ACGACGGCGAACATCAACGACTGCAGCAACGTCTCTGGCGCGATCCTCGGTATCGTCCAGCGTCTGGACGACGCGGTGGA
TCTGGTCGTCAGCGGCCATACGCACCAGGCGTATCGGTGCAGCCTGTCCAACCGCGCAGGCCGCACTATCCCGGTGACGA
GCACCAACGCCCAGGGGCGCTCCGTCACCGAGATCGATCTGGTCATCGACACCATGACGCGCGACGTGGCCAGCATTGCC
GTCGACAACGTGGTGGTCGACCGCAGCAATGCCGCCGTGCCTCCCATGGCGGCGCTGACCGCGCTGGCCAACGGCTACAA
TGCGTTGGCCGCGCCGCTGGCCAATACGCCCCTTGGCGGCATCACGGCCGCGATCACCCGCAGCGCCAATGCGGCGGGCG
AGTCCGCGCTGGGCGACGTCATCGCCGACGCGCAGCTCGAAGCGACTGCCGCTCCGGAACTGGGCGGTGCGGTGATTGCC
CTGATGAACCCGGGCGGCATACGCGCCGACTTCACCTACCCGAGCAGCGACGCGCTGGAAGGCGACGGCGTCGTGACTTA
TGGTGAAGCGTTCGCCGTGCAGCCTTTCGGCAACTCACTGGTGACCAAAACCTTCACGGGCCAGCAGATTTATGATCTGC
TGGAGCAGCAGTGGGGCGCGTTCCAACCGTTCGCGCGCATCCTCCAGGTGTCGGAAGGCTTTAGCTACCAGCACACGTTC
GACACCGCGGCGGCGAACTTCGCAGCACAAAAAGGCGCCCACTACGTCTGCGACGGCAGCGTCAAGCTCCATGGCGTGGC
GATCGACAAGCTCGCCAGCTACCGCGTGACCATGAACTCCTTCCTCGCCTCGGGCGGCGACAATTTCACCGTGTTCACTC
AGGGGACGGGCCAACTCGGTGGCGCCCTTGACCTCGACGCGATGCAGCAGTATTTCAAGTCGCACGATCCCGTCGCACCC
AGCGCGCAGGACCGCGTCGTCAAGGTGAGTGTCTGCCCGGCGCCATGA

Upstream 100 bases:

>100_bases
ACAGTTTTTTCAAAGCGCGTTTTGTCATCTTGATTCTCCAGAGTGACGGCTGAACCGTGCGAGTCCCTCGCATGCCCGCT
GTCCACCCTGGAGAAAAAAC

Downstream 100 bases:

>100_bases
TGCGGTAGCCGGCTGCGGGAAGGCGAGCGTGGGGCTCGCTTTGTCTGTCCCGCGTTGCGCCGGCGCGGAAGGGGAGATGG
TCCGTTTGGGCCATGTCCGC

Product: 5'-nucleotidase

Products: ribonucleoside; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MMHRLHPLAVGVLLSLSVQAQAACLEPSVVIGTVDSAVANKEIGAACMNDLIIDSASEGANWGSHGEFVSAVSRLTQDWL
KAESISATERSRIVSAAARSDVGSTLLVKIAAINDFHGQLFSPGSFAGKPVGGIDALAAYIDAIRAKSPNHVTVSAGDLI
GATPLVSALFHDEPTIEGMNILGLDFNAVGNHEFDEGRDELLRMQNGGCHPTDPNSCQGDAVGTPVPFEGARFKFLAANV
TEKTDGKTLFPAYAIRNFKGNKVAFIGMTLEDTPSIVTPSGISTLDFHDEADTVNALIPELKAQGVESIVVLVHEGGVDT
TTANINDCSNVSGAILGIVQRLDDAVDLVVSGHTHQAYRCSLSNRAGRTIPVTSTNAQGRSVTEIDLVIDTMTRDVASIA
VDNVVVDRSNAAVPPMAALTALANGYNALAAPLANTPLGGITAAITRSANAAGESALGDVIADAQLEATAAPELGGAVIA
LMNPGGIRADFTYPSSDALEGDGVVTYGEAFAVQPFGNSLVTKTFTGQQIYDLLEQQWGAFQPFARILQVSEGFSYQHTF
DTAAANFAAQKGAHYVCDGSVKLHGVAIDKLASYRVTMNSFLASGGDNFTVFTQGTGQLGGALDLDAMQQYFKSHDPVAP
SAQDRVVKVSVCPAP

Sequences:

>Translated_655_residues
MMHRLHPLAVGVLLSLSVQAQAACLEPSVVIGTVDSAVANKEIGAACMNDLIIDSASEGANWGSHGEFVSAVSRLTQDWL
KAESISATERSRIVSAAARSDVGSTLLVKIAAINDFHGQLFSPGSFAGKPVGGIDALAAYIDAIRAKSPNHVTVSAGDLI
GATPLVSALFHDEPTIEGMNILGLDFNAVGNHEFDEGRDELLRMQNGGCHPTDPNSCQGDAVGTPVPFEGARFKFLAANV
TEKTDGKTLFPAYAIRNFKGNKVAFIGMTLEDTPSIVTPSGISTLDFHDEADTVNALIPELKAQGVESIVVLVHEGGVDT
TTANINDCSNVSGAILGIVQRLDDAVDLVVSGHTHQAYRCSLSNRAGRTIPVTSTNAQGRSVTEIDLVIDTMTRDVASIA
VDNVVVDRSNAAVPPMAALTALANGYNALAAPLANTPLGGITAAITRSANAAGESALGDVIADAQLEATAAPELGGAVIA
LMNPGGIRADFTYPSSDALEGDGVVTYGEAFAVQPFGNSLVTKTFTGQQIYDLLEQQWGAFQPFARILQVSEGFSYQHTF
DTAAANFAAQKGAHYVCDGSVKLHGVAIDKLASYRVTMNSFLASGGDNFTVFTQGTGQLGGALDLDAMQQYFKSHDPVAP
SAQDRVVKVSVCPAP
>Mature_655_residues
MMHRLHPLAVGVLLSLSVQAQAACLEPSVVIGTVDSAVANKEIGAACMNDLIIDSASEGANWGSHGEFVSAVSRLTQDWL
KAESISATERSRIVSAAARSDVGSTLLVKIAAINDFHGQLFSPGSFAGKPVGGIDALAAYIDAIRAKSPNHVTVSAGDLI
GATPLVSALFHDEPTIEGMNILGLDFNAVGNHEFDEGRDELLRMQNGGCHPTDPNSCQGDAVGTPVPFEGARFKFLAANV
TEKTDGKTLFPAYAIRNFKGNKVAFIGMTLEDTPSIVTPSGISTLDFHDEADTVNALIPELKAQGVESIVVLVHEGGVDT
TTANINDCSNVSGAILGIVQRLDDAVDLVVSGHTHQAYRCSLSNRAGRTIPVTSTNAQGRSVTEIDLVIDTMTRDVASIA
VDNVVVDRSNAAVPPMAALTALANGYNALAAPLANTPLGGITAAITRSANAAGESALGDVIADAQLEATAAPELGGAVIA
LMNPGGIRADFTYPSSDALEGDGVVTYGEAFAVQPFGNSLVTKTFTGQQIYDLLEQQWGAFQPFARILQVSEGFSYQHTF
DTAAANFAAQKGAHYVCDGSVKLHGVAIDKLASYRVTMNSFLASGGDNFTVFTQGTGQLGGALDLDAMQQYFKSHDPVAP
SAQDRVVKVSVCPAP

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786687, Length=582, Percent_Identity=23.0240549828179, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI221329836, Length=586, Percent_Identity=24.061433447099, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24641187, Length=581, Percent_Identity=24.0963855421687, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922444, Length=543, Percent_Identity=25.0460405156538, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922446, Length=557, Percent_Identity=24.5960502692998, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654424, Length=557, Percent_Identity=24.5960502692998, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.1.3.5

Molecular weight: Translated: 68257; Mature: 68257

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHRLHPLAVGVLLSLSVQAQAACLEPSVVIGTVDSAVANKEIGAACMNDLIIDSASEGA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHEECCCCCC
NWGSHGEFVSAVSRLTQDWLKAESISATERSRIVSAAARSDVGSTLLVKIAAINDFHGQL
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC
FSPGSFAGKPVGGIDALAAYIDAIRAKSPNHVTVSAGDLIGATPLVSALFHDEPTIEGMN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCE
ILGLDFNAVGNHEFDEGRDELLRMQNGGCHPTDPNSCQGDAVGTPVPFEGARFKFLAANV
EEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
TEKTDGKTLFPAYAIRNFKGNKVAFIGMTLEDTPSIVTPSGISTLDFHDEADTVNALIPE
CCCCCCCEECHHHHHHCCCCCEEEEEEEEECCCCCEECCCCCEEEECCCCHHHHHHHHHH
LKAQGVESIVVLVHEGGVDTTTANINDCSNVSGAILGIVQRLDDAVDLVVSGHTHQAYRC
HHHCCCEEEEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEE
SLSNRAGRTIPVTSTNAQGRSVTEIDLVIDTMTRDVASIAVDNVVVDRSNAAVPPMAALT
ECCCCCCCEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHH
ALANGYNALAAPLANTPLGGITAAITRSANAAGESALGDVIADAQLEATAAPELGGAVIA
HHHCCCHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCEECCCCCCCCCCEEEE
LMNPGGIRADFTYPSSDALEGDGVVTYGEAFAVQPFGNSLVTKTFTGQQIYDLLEQQWGA
EECCCCEEEEECCCCCCCCCCCCEEEECCEEEECCCCCCEEEEECCHHHHHHHHHHHCCC
FQPFARILQVSEGFSYQHTFDTAAANFAAQKGAHYVCDGSVKLHGVAIDKLASYRVTMNS
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCEEEECCCEEEEEEEHHHHHHHHEEHHH
FLASGGDNFTVFTQGTGQLGGALDLDAMQQYFKSHDPVAPSAQDRVVKVSVCPAP
HHHCCCCCEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MMHRLHPLAVGVLLSLSVQAQAACLEPSVVIGTVDSAVANKEIGAACMNDLIIDSASEGA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHEECCCCCC
NWGSHGEFVSAVSRLTQDWLKAESISATERSRIVSAAARSDVGSTLLVKIAAINDFHGQL
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCC
FSPGSFAGKPVGGIDALAAYIDAIRAKSPNHVTVSAGDLIGATPLVSALFHDEPTIEGMN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCE
ILGLDFNAVGNHEFDEGRDELLRMQNGGCHPTDPNSCQGDAVGTPVPFEGARFKFLAANV
EEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
TEKTDGKTLFPAYAIRNFKGNKVAFIGMTLEDTPSIVTPSGISTLDFHDEADTVNALIPE
CCCCCCCEECHHHHHHCCCCCEEEEEEEEECCCCCEECCCCCEEEECCCCHHHHHHHHHH
LKAQGVESIVVLVHEGGVDTTTANINDCSNVSGAILGIVQRLDDAVDLVVSGHTHQAYRC
HHHCCCEEEEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEE
SLSNRAGRTIPVTSTNAQGRSVTEIDLVIDTMTRDVASIAVDNVVVDRSNAAVPPMAALT
ECCCCCCCEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHH
ALANGYNALAAPLANTPLGGITAAITRSANAAGESALGDVIADAQLEATAAPELGGAVIA
HHHCCCHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCEECCCCCCCCCCEEEE
LMNPGGIRADFTYPSSDALEGDGVVTYGEAFAVQPFGNSLVTKTFTGQQIYDLLEQQWGA
EECCCCEEEEECCCCCCCCCCCCEEEECCEEEECCCCCCEEEEECCHHHHHHHHHHHCCC
FQPFARILQVSEGFSYQHTFDTAAANFAAQKGAHYVCDGSVKLHGVAIDKLASYRVTMNS
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCEEEECCCEEEEEEEHHHHHHHHEEHHH
FLASGGDNFTVFTQGTGQLGGALDLDAMQQYFKSHDPVAPSAQDRVVKVSVCPAP
HHHCCCCCEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5'-ribonucleotide; H2O

Specific reaction: A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]