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The map label for this gene is yfgF [C]

Identifier: 74316368

GI number: 74316368

Start: 367902

End: 369722

Strand: Reverse

Name: yfgF [C]

Synonym: Tbd_0350

Alternate gene names: 74316368

Gene position: 369722-367902 (Counterclockwise)

Preceding gene: 74316381

Following gene: 74316367

Centisome position: 12.71

GC content: 61.67

Gene sequence:

>1821_bases
ATGAGCCCCGACGATATGCGCTCCCATCTGGAAGAAATTCTTGCAACGGCACGCCTGACAACTCTGCTTCAGCCGGTCCT
CGACATGTCGAAGGGGGTGGTCAGCGGATATGAGGCACTCTCCCGCGGACCGTCGGACAGCCCGCTGCATGCGCCGCAGA
CCTTGTTCCGGGTGGCTGAAACGCACGGGCTCGGCACACAGCTTGAATGGGCATGCGTACGCTCGGCCTTCGCGACCTTC
ACTACGCTCGGTCTTCCCGGACAACTGTTCGTCAATCTGTCGCCGGCAAGCCTGCTCGACCCCGCCTTCGCACCCGCGGC
CGTTGTCGCGGCGCTCAAGGGGGCCGACCTCGCGAATGACCGTCTGGTTTTCGAGATCACTGAACATTCCGCGCACGTCG
ACTATGGCAGCCTTCGCGACGCGGTCCGCGCGCTGCGCTCAGAGGGGATCGACATCGCGATCGATGATCTGGGCGAAGGT
TTTTCCAGCCTTCGGTTGTGGTCAGAATTGAAGCCCGCCTACGTCAAGATCGACAAACACTTCATTTCCGGAATTCATCA
GGATCCTCACAAGATCCAGTTTGTGCGTTCGATTCGCCAGCTCGCCGAGGGCGCGCATTCGTGCGTGATTGCCGAGGGCG
TGGAAAATCTGTCCGAACTCGCCGTGCTCAAGGACCTCGGAATGCGCTATGTACAGGGCTACCTCATCGGTCGCCCGTCG
GCGGATCCGATCCGTCTTCTTCCGCGCGAGGTCGAGGCCTGCCTGCAATCTGGCGTGGTAAGCGTCCTGCCATGGTCACC
GATGCGGGAAACGCTTCACGCGTCGGCGGACCGGCTCGTCGTTCCAGCGCCCGCCGTAAGTCCCGCAACGGTGAGCGAGG
ACGTGCTCGAACTCATGATGCTTCGCCCCGACCTGCACGCTCTGGCCGTAGTCGACAATGAGATTCCCGTCGGCATCATT
CACCGCCCCGCGCTGCTGGACCGTTTCATCAGCCTCTACGGGCGGGAACTTCACGGTCGCAAGCCCTGCTCGGCCCTGAT
GGATGCCGATCCGCTGATTGTCGACAAGGCCACCCGTATGAGCGAACTCTCCGAACTCGTCATTCAGAAAGGCAAGGCTG
CCTTCACGCAAGGCTTCATCATCACGGACGAGGGCCGTTACCTGGGCCTGGGATCTGGGTTCGATCTGATGCGCGAGGTG
ACGGAAATGCAGATCGTCGCCGCGCGCTATGCCAATCCACTTACGGGGCTGCCCGGCAATGTCCCGATTCAGGAACACCT
GGAGCGGCTACTGACGAATTCGCAGACCTTTGTCGCCGCCTATTTCGATCTCGATCACTTCAAGCCTTACAACGACGTCT
ACGGGTTTCGGCGCGGCGACGACATCATTCAGCTCCTCGCGCACATCCTCTGCGATGGCTCCAACCCTGAACTGGACTTC
GTCGGGCATATCGGCGGTGACGATTTTGTCGTCCTGTTCCAGAGCCTGGACTGGGAGGAGCGTTGCCGTACCGTGTTGTC
CCGCTTCGACGAGGAACGGCTCGCCCTGTTCCTCCCCGAACACGTCCTGTCGTCCGGTTACCACACCGAGGACAGGCGCG
GCGTCATGACCTTTCACGGCCTCGTGACACTGTCGATCGGAGCGGTCTTCGTCGACCCGACGCACTTCCATCATCACCAG
GAGGTAGCGCGCGCAGCCTCGGACGCCAAACGCATGGCGAAGCGCGTCGAGGGAAACAGCCTCTTCATCGACCAGCGCCG
CGTTCCCTTCCGGCGGACGTTAGTACTGGAACCCACGGTGCCCGAGGCGAATTGCGCGTGA

Upstream 100 bases:

>100_bases
ACATCCGGAATGACACGGCCGTTACAGGCATCGAACGAATTCACATCTCGGTAACGCAAGCTCCGTACGCTTCGCGCGAT
TTGACCCTCCGTTTTCGTTC

Downstream 100 bases:

>100_bases
AAGAAATGTATGGTCTCGCCGTATATCCGGTCCTTGGCGGAAACAGCCGCCCGAAGGCCGCTTTTCCGCTTTCGGCGCAT
GCCATCCGCCACGAGAGGTG

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MSPDDMRSHLEEILATARLTTLLQPVLDMSKGVVSGYEALSRGPSDSPLHAPQTLFRVAETHGLGTQLEWACVRSAFATF
TTLGLPGQLFVNLSPASLLDPAFAPAAVVAALKGADLANDRLVFEITEHSAHVDYGSLRDAVRALRSEGIDIAIDDLGEG
FSSLRLWSELKPAYVKIDKHFISGIHQDPHKIQFVRSIRQLAEGAHSCVIAEGVENLSELAVLKDLGMRYVQGYLIGRPS
ADPIRLLPREVEACLQSGVVSVLPWSPMRETLHASADRLVVPAPAVSPATVSEDVLELMMLRPDLHALAVVDNEIPVGII
HRPALLDRFISLYGRELHGRKPCSALMDADPLIVDKATRMSELSELVIQKGKAAFTQGFIITDEGRYLGLGSGFDLMREV
TEMQIVAARYANPLTGLPGNVPIQEHLERLLTNSQTFVAAYFDLDHFKPYNDVYGFRRGDDIIQLLAHILCDGSNPELDF
VGHIGGDDFVVLFQSLDWEERCRTVLSRFDEERLALFLPEHVLSSGYHTEDRRGVMTFHGLVTLSIGAVFVDPTHFHHHQ
EVARAASDAKRMAKRVEGNSLFIDQRRVPFRRTLVLEPTVPEANCA

Sequences:

>Translated_606_residues
MSPDDMRSHLEEILATARLTTLLQPVLDMSKGVVSGYEALSRGPSDSPLHAPQTLFRVAETHGLGTQLEWACVRSAFATF
TTLGLPGQLFVNLSPASLLDPAFAPAAVVAALKGADLANDRLVFEITEHSAHVDYGSLRDAVRALRSEGIDIAIDDLGEG
FSSLRLWSELKPAYVKIDKHFISGIHQDPHKIQFVRSIRQLAEGAHSCVIAEGVENLSELAVLKDLGMRYVQGYLIGRPS
ADPIRLLPREVEACLQSGVVSVLPWSPMRETLHASADRLVVPAPAVSPATVSEDVLELMMLRPDLHALAVVDNEIPVGII
HRPALLDRFISLYGRELHGRKPCSALMDADPLIVDKATRMSELSELVIQKGKAAFTQGFIITDEGRYLGLGSGFDLMREV
TEMQIVAARYANPLTGLPGNVPIQEHLERLLTNSQTFVAAYFDLDHFKPYNDVYGFRRGDDIIQLLAHILCDGSNPELDF
VGHIGGDDFVVLFQSLDWEERCRTVLSRFDEERLALFLPEHVLSSGYHTEDRRGVMTFHGLVTLSIGAVFVDPTHFHHHQ
EVARAASDAKRMAKRVEGNSLFIDQRRVPFRRTLVLEPTVPEANCA
>Mature_605_residues
SPDDMRSHLEEILATARLTTLLQPVLDMSKGVVSGYEALSRGPSDSPLHAPQTLFRVAETHGLGTQLEWACVRSAFATFT
TLGLPGQLFVNLSPASLLDPAFAPAAVVAALKGADLANDRLVFEITEHSAHVDYGSLRDAVRALRSEGIDIAIDDLGEGF
SSLRLWSELKPAYVKIDKHFISGIHQDPHKIQFVRSIRQLAEGAHSCVIAEGVENLSELAVLKDLGMRYVQGYLIGRPSA
DPIRLLPREVEACLQSGVVSVLPWSPMRETLHASADRLVVPAPAVSPATVSEDVLELMMLRPDLHALAVVDNEIPVGIIH
RPALLDRFISLYGRELHGRKPCSALMDADPLIVDKATRMSELSELVIQKGKAAFTQGFIITDEGRYLGLGSGFDLMREVT
EMQIVAARYANPLTGLPGNVPIQEHLERLLTNSQTFVAAYFDLDHFKPYNDVYGFRRGDDIIQLLAHILCDGSNPELDFV
GHIGGDDFVVLFQSLDWEERCRTVLSRFDEERLALFLPEHVLSSGYHTEDRRGVMTFHGLVTLSIGAVFVDPTHFHHHQE
VARAASDAKRMAKRVEGNSLFIDQRRVPFRRTLVLEPTVPEANCA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=237, Percent_Identity=29.957805907173, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1787055, Length=233, Percent_Identity=30.4721030042918, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87082096, Length=150, Percent_Identity=35.3333333333333, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081921, Length=239, Percent_Identity=26.7782426778243, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790496, Length=238, Percent_Identity=27.7310924369748, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081845, Length=241, Percent_Identity=28.2157676348548, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI226510982, Length=224, Percent_Identity=29.9107142857143, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787410, Length=226, Percent_Identity=23.8938053097345, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI87081743, Length=245, Percent_Identity=24.8979591836735, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787541, Length=237, Percent_Identity=28.6919831223629, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786507, Length=233, Percent_Identity=25.7510729613734, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788502, Length=225, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081980, Length=132, Percent_Identity=34.0909090909091, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT; PF00989 PAS [H]

EC number: NA

Molecular weight: Translated: 66899; Mature: 66768

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPDDMRSHLEEILATARLTTLLQPVLDMSKGVVSGYEALSRGPSDSPLHAPQTLFRVAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
THGLGTQLEWACVRSAFATFTTLGLPGQLFVNLSPASLLDPAFAPAAVVAALKGADLAND
HCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHCCCCCCC
RLVFEITEHSAHVDYGSLRDAVRALRSEGIDIAIDDLGEGFSSLRLWSELKPAYVKIDKH
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHCCCHHEEHHHH
FISGIHQDPHKIQFVRSIRQLAEGAHSCVIAEGVENLSELAVLKDLGMRYVQGYLIGRPS
HHHCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
ADPIRLLPREVEACLQSGVVSVLPWSPMRETLHASADRLVVPAPAVSPATVSEDVLELMM
CCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHH
LRPDLHALAVVDNEIPVGIIHRPALLDRFISLYGRELHGRKPCSALMDADPLIVDKATRM
HCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEECHHHH
SELSELVIQKGKAAFTQGFIITDEGRYLGLGSGFDLMREVTEMQIVAARYANPLTGLPGN
HHHHHHHHHCCCHHHHCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
VPIQEHLERLLTNSQTFVAAYFDLDHFKPYNDVYGFRRGDDIIQLLAHILCDGSNPELDF
CCHHHHHHHHHCCCCEEEEEEEEHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEE
VGHIGGDDFVVLFQSLDWEERCRTVLSRFDEERLALFLPEHVLSSGYHTEDRRGVMTFHG
EEECCCCCEEEEEHHCCHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCCCCCCCCEEHHHH
LVTLSIGAVFVDPTHFHHHQEVARAASDAKRMAKRVEGNSLFIDQRRVPFRRTLVLEPTV
HHHEEECEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHEEEECCCC
PEANCA
CCCCCC
>Mature Secondary Structure 
SPDDMRSHLEEILATARLTTLLQPVLDMSKGVVSGYEALSRGPSDSPLHAPQTLFRVAE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
THGLGTQLEWACVRSAFATFTTLGLPGQLFVNLSPASLLDPAFAPAAVVAALKGADLAND
HCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHCCCCCCC
RLVFEITEHSAHVDYGSLRDAVRALRSEGIDIAIDDLGEGFSSLRLWSELKPAYVKIDKH
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHCCCHHEEHHHH
FISGIHQDPHKIQFVRSIRQLAEGAHSCVIAEGVENLSELAVLKDLGMRYVQGYLIGRPS
HHHCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
ADPIRLLPREVEACLQSGVVSVLPWSPMRETLHASADRLVVPAPAVSPATVSEDVLELMM
CCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHH
LRPDLHALAVVDNEIPVGIIHRPALLDRFISLYGRELHGRKPCSALMDADPLIVDKATRM
HCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEECHHHH
SELSELVIQKGKAAFTQGFIITDEGRYLGLGSGFDLMREVTEMQIVAARYANPLTGLPGN
HHHHHHHHHCCCHHHHCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
VPIQEHLERLLTNSQTFVAAYFDLDHFKPYNDVYGFRRGDDIIQLLAHILCDGSNPELDF
CCHHHHHHHHHCCCCEEEEEEEEHHCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEE
VGHIGGDDFVVLFQSLDWEERCRTVLSRFDEERLALFLPEHVLSSGYHTEDRRGVMTFHG
EEECCCCCEEEEEHHCCHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCCCCCCCCEEHHHH
LVTLSIGAVFVDPTHFHHHQEVARAASDAKRMAKRVEGNSLFIDQRRVPFRRTLVLEPTV
HHHEEECEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHEEEECCCC
PEANCA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]