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The map label for this gene is asnB [H]

Identifier: 74316316

GI number: 74316316

Start: 319069

End: 320889

Strand: Reverse

Name: asnB [H]

Synonym: Tbd_0298

Alternate gene names: 74316316

Gene position: 320889-319069 (Counterclockwise)

Preceding gene: 74316324

Following gene: 74316298

Centisome position: 11.03

GC content: 68.81

Gene sequence:

>1821_bases
ATGGATGGCATTTTTGGCTGGCTAGGCGCCGACGGCGCGCATCAGGACCTGATCCGGCAGATCGCGGGCGGCGATGCCGA
CGCGGCCGGCCTGCGTCGATACCACAGCGCGGAACTCGGACTGGCGGCGCAGTCGCGCTTCGGCAAGGCCGACATCGCGG
TCGAGGATGGACACGCGGTGGCGCTCATGGGACGCCCGCGCTTCGTCGACGCGGACCTCGCGCGCGTCGCCCGCGAAACC
GGCCCGGCCGCCGCGGTCGCGCAGGGCTGGCGCAGCCACGGCGCCGGCCTGCCGGCGCGCATGCAGGGGAATTTCGCCTT
CGCCGTGCTCGAGCCGCAGGCGAAGAAGGCCTGCCTCGTGCTCGACCGCATGGGTGCGGAACGCCTGGGCTACGCGCATC
GCGGCGACCAGCTCGTTTTCGGCAGCAGCGTCCAGCACGTCGCCGCCCACCCTGCCGTCGGCCGCAACATCGAGCCGCAG
GCGATCTACGACTACCTGTACTTCCACAACATCCCCGCGCCGCGCAGCCTCTACCGCGGCGTGCACAAGCTGCTGCCCGG
ACAGATCGTCACGCTCGAGAACGGACGGCTCGCGACGCGCTTCTACTGGCACGCCGATTACACGCCGCACGACGCGGGCT
TCGACGTGCAGCGCGACGCCTTCCGTCGCGTCCTCGACGCGGCCGTGCGCGACGCCGACGGGCCGGCGACCGCAGCGTTT
CTCTCGGGCGGCACCGACAGTTCGACGGTCGTCGGCGCGCTGACGGCGGCGCGCGGCGGGCCCGTCGATACCTTCTCGAT
CGGTTTCGACGCCGAGGGCTTCGACGAAATGGCGTATGCCCGCTGTGCCGCCGCGCGGTACGACAGCCGGCGCCACGAGT
ACTACCTCAAGCCGGCCGACATCGTCACCGCGATTCCGCTGATCGCGCGCGAATACGACGAGCCCTTCGGCAACGACTCC
GCGGTCCCGACCTATTTCTGCGCCCGCGCCGCGCGCGAAGCGGGCTTCGGACGCATGCTCGCCGGCGACGGCGGCGACGA
GATATTCGGCGGCAACGCGCGCTACGCGAAGCAGAAGCTGTTCGAAAATTATTTCCGCGTGCCGGCGCCGCTGCGCCACG
GCCTGATCGAGCCGCTGGCGACGCTTCCGGGCGTGTCCGATCACTTTCCGCTGTCCAAGCTCAAGAGCTACGTCCGCCAG
GCGAACATGGGGCTGCCGCTGCGCATGGAAAGCTACAACTTCCTGCACCGCACGCCGCTCGACCAGATCTTCGCCGCCGA
TTTCGTCGCCGCGGTCGATCCTTCGGCGGCGGATGCAGCGCTCACCGAGGTCTACGCGCGCACGGCGTCGGACCACTACA
TCAACCGCATGATGCATCTCGACCTCAAGTTCACGCTCGCCGACAACGACCTGCGCAAGGTCGGCACGATGACCGAGGCG
GCGGGGGTCGAGGTCGCCTACCCGCTGCTCGACGACCGCCTGGTCGCGCTCGCGAACCATCTTCCGGTCGACTGGAAGGT
ACGCGGACAAGCGCTGCGCTGGTTCTTCAAGGAAGCCCTGCGCGACCTGCTGCCGGAAGAGATCATCAACAAGCGCAAGC
ACGGCTTCGGCCTGCCGTTCGGCGTATGGAGCACACAACACGCGCCGCTCGCCGAACTCGTGCACGACAGTCTCTCGGAC
TTCAAGCGCCGTGGCTGGGTCCAGCCGGGCTACCTCGACCACATGCTCGCGATGCAGCGTGGCCCGCACGCGAGCTACTA
CGGCGTCATGATCTGGGTCGTGATGATGCTCGAGCGCTGGCTGCAGGCGAACGCGCACTAG

Upstream 100 bases:

>100_bases
TGCCCCGTCCAGGCGGACGGGTCCGACAGGATTCGTGCCAACCTGCCGGGCTCGTTTATATTGGCGCCCATTATCCCGTA
TCGCTTCGACCGCACACACG

Downstream 100 bases:

>100_bases
CGCCGGTTCCACGCTGCGCTACCCCGCCGCGGCCTCGCGCCGCTTGCCGAATCCCCCCACGGGCACCGGCTGTTTCTGCA
GCACGCCGGAGAATTTGGCG

Product: amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MDGIFGWLGADGAHQDLIRQIAGGDADAAGLRRYHSAELGLAAQSRFGKADIAVEDGHAVALMGRPRFVDADLARVARET
GPAAAVAQGWRSHGAGLPARMQGNFAFAVLEPQAKKACLVLDRMGAERLGYAHRGDQLVFGSSVQHVAAHPAVGRNIEPQ
AIYDYLYFHNIPAPRSLYRGVHKLLPGQIVTLENGRLATRFYWHADYTPHDAGFDVQRDAFRRVLDAAVRDADGPATAAF
LSGGTDSSTVVGALTAARGGPVDTFSIGFDAEGFDEMAYARCAAARYDSRRHEYYLKPADIVTAIPLIAREYDEPFGNDS
AVPTYFCARAAREAGFGRMLAGDGGDEIFGGNARYAKQKLFENYFRVPAPLRHGLIEPLATLPGVSDHFPLSKLKSYVRQ
ANMGLPLRMESYNFLHRTPLDQIFAADFVAAVDPSAADAALTEVYARTASDHYINRMMHLDLKFTLADNDLRKVGTMTEA
AGVEVAYPLLDDRLVALANHLPVDWKVRGQALRWFFKEALRDLLPEEIINKRKHGFGLPFGVWSTQHAPLAELVHDSLSD
FKRRGWVQPGYLDHMLAMQRGPHASYYGVMIWVVMMLERWLQANAH

Sequences:

>Translated_606_residues
MDGIFGWLGADGAHQDLIRQIAGGDADAAGLRRYHSAELGLAAQSRFGKADIAVEDGHAVALMGRPRFVDADLARVARET
GPAAAVAQGWRSHGAGLPARMQGNFAFAVLEPQAKKACLVLDRMGAERLGYAHRGDQLVFGSSVQHVAAHPAVGRNIEPQ
AIYDYLYFHNIPAPRSLYRGVHKLLPGQIVTLENGRLATRFYWHADYTPHDAGFDVQRDAFRRVLDAAVRDADGPATAAF
LSGGTDSSTVVGALTAARGGPVDTFSIGFDAEGFDEMAYARCAAARYDSRRHEYYLKPADIVTAIPLIAREYDEPFGNDS
AVPTYFCARAAREAGFGRMLAGDGGDEIFGGNARYAKQKLFENYFRVPAPLRHGLIEPLATLPGVSDHFPLSKLKSYVRQ
ANMGLPLRMESYNFLHRTPLDQIFAADFVAAVDPSAADAALTEVYARTASDHYINRMMHLDLKFTLADNDLRKVGTMTEA
AGVEVAYPLLDDRLVALANHLPVDWKVRGQALRWFFKEALRDLLPEEIINKRKHGFGLPFGVWSTQHAPLAELVHDSLSD
FKRRGWVQPGYLDHMLAMQRGPHASYYGVMIWVVMMLERWLQANAH
>Mature_606_residues
MDGIFGWLGADGAHQDLIRQIAGGDADAAGLRRYHSAELGLAAQSRFGKADIAVEDGHAVALMGRPRFVDADLARVARET
GPAAAVAQGWRSHGAGLPARMQGNFAFAVLEPQAKKACLVLDRMGAERLGYAHRGDQLVFGSSVQHVAAHPAVGRNIEPQ
AIYDYLYFHNIPAPRSLYRGVHKLLPGQIVTLENGRLATRFYWHADYTPHDAGFDVQRDAFRRVLDAAVRDADGPATAAF
LSGGTDSSTVVGALTAARGGPVDTFSIGFDAEGFDEMAYARCAAARYDSRRHEYYLKPADIVTAIPLIAREYDEPFGNDS
AVPTYFCARAAREAGFGRMLAGDGGDEIFGGNARYAKQKLFENYFRVPAPLRHGLIEPLATLPGVSDHFPLSKLKSYVRQ
ANMGLPLRMESYNFLHRTPLDQIFAADFVAAVDPSAADAALTEVYARTASDHYINRMMHLDLKFTLADNDLRKVGTMTEA
AGVEVAYPLLDDRLVALANHLPVDWKVRGQALRWFFKEALRDLLPEEIINKRKHGFGLPFGVWSTQHAPLAELVHDSLSD
FKRRGWVQPGYLDHMLAMQRGPHASYYGVMIWVVMMLERWLQANAH

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 66773; Mature: 66773

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGIFGWLGADGAHQDLIRQIAGGDADAAGLRRYHSAELGLAAQSRFGKADIAVEDGHAV
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCCEEEECCCEE
ALMGRPRFVDADLARVARETGPAAAVAQGWRSHGAGLPARMQGNFAFAVLEPQAKKACLV
EEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCCEEEEEECCCCHHHHHH
LDRMGAERLGYAHRGDQLVFGSSVQHVAAHPAVGRNIEPQAIYDYLYFHNIPAPRSLYRG
HHHHCHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
VHKLLPGQIVTLENGRLATRFYWHADYTPHDAGFDVQRDAFRRVLDAAVRDADGPATAAF
HHHHCCCEEEEECCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHE
LSGGTDSSTVVGALTAARGGPVDTFSIGFDAEGFDEMAYARCAAARYDSRRHEYYLKPAD
ECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHH
IVTAIPLIAREYDEPFGNDSAVPTYFCARAAREAGFGRMLAGDGGDEIFGGNARYAKQKL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHH
FENYFRVPAPLRHGLIEPLATLPGVSDHFPLSKLKSYVRQANMGLPLRMESYNFLHRTPL
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHCCCH
DQIFAADFVAAVDPSAADAALTEVYARTASDHYINRMMHLDLKFTLADNDLRKVGTMTEA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHCHHHH
AGVEVAYPLLDDRLVALANHLPVDWKVRGQALRWFFKEALRDLLPEEIINKRKHGFGLPF
CCCEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCC
GVWSTQHAPLAELVHDSLSDFKRRGWVQPGYLDHMLAMQRGPHASYYGVMIWVVMMLERW
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LQANAH
HHCCCC
>Mature Secondary Structure
MDGIFGWLGADGAHQDLIRQIAGGDADAAGLRRYHSAELGLAAQSRFGKADIAVEDGHAV
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCCCEEEECCCEE
ALMGRPRFVDADLARVARETGPAAAVAQGWRSHGAGLPARMQGNFAFAVLEPQAKKACLV
EEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCCEEEEEECCCCHHHHHH
LDRMGAERLGYAHRGDQLVFGSSVQHVAAHPAVGRNIEPQAIYDYLYFHNIPAPRSLYRG
HHHHCHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
VHKLLPGQIVTLENGRLATRFYWHADYTPHDAGFDVQRDAFRRVLDAAVRDADGPATAAF
HHHHCCCEEEEECCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHE
LSGGTDSSTVVGALTAARGGPVDTFSIGFDAEGFDEMAYARCAAARYDSRRHEYYLKPAD
ECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHH
IVTAIPLIAREYDEPFGNDSAVPTYFCARAAREAGFGRMLAGDGGDEIFGGNARYAKQKL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHH
FENYFRVPAPLRHGLIEPLATLPGVSDHFPLSKLKSYVRQANMGLPLRMESYNFLHRTPL
HHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHCCCH
DQIFAADFVAAVDPSAADAALTEVYARTASDHYINRMMHLDLKFTLADNDLRKVGTMTEA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHCHHHH
AGVEVAYPLLDDRLVALANHLPVDWKVRGQALRWFFKEALRDLLPEEIINKRKHGFGLPF
CCCEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCC
GVWSTQHAPLAELVHDSLSDFKRRGWVQPGYLDHMLAMQRGPHASYYGVMIWVVMMLERW
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LQANAH
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]