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The map label for this gene is lipA

Identifier: 74316292

GI number: 74316292

Start: 293125

End: 294060

Strand: Direct

Name: lipA

Synonym: Tbd_0274

Alternate gene names: 74316292

Gene position: 293125-294060 (Clockwise)

Preceding gene: 74316289

Following gene: 74316299

Centisome position: 10.07

GC content: 67.95

Gene sequence:

>936_bases
ATGGCAGACCCCCTCCAGCACAAAGGCGCGGCGAAGACGTCGCGCATCCCGATCAAGATCGTGCCGCAAGAGCGGCAACG
CCTGCCGCCGTGGATCCGGGCGAAGGCGCCGAGCCTACCGAACGTCGGACGGCTCAAGGGCATCCTGCGCGAAGCCAAGC
TGCACACGGTGTGCGAGGAGGCGTCGTGCCCGAATCTCGGCGAGTGCTTCGGCCACGGCACGGCGACTTTCATGATCCTT
GGCGACCTCTGCACGCGGCGTTGCCCGTTCTGTGACGTCGGCCACGGCACGCCCTTGCCGCCGGACGCCGACGAGCCGCG
TCATCTCGCCGAAACGATCGCGCTGATGGCCTTGAAGTACGTTGTCATCACGAGCGTCGACCGCGACGACCTGCGTGACG
GCGGCGCCGGGCATTTTGCCGAGTGCATCGCGGCCGTGCGCGAAAAATCGCCGGCGACGCGGATCGAGATACTGACTCCC
GATTTTCGTGGCCGCCTCGACAAGGCGCTCGCGGCGCTCGACCGTGCCCCGCCCGACGTCATGAACCACAACCTCGAAAC
CGTGCCGCGCCTCTACAAGGCCGCGCGCCCGGGCGCCGACTACGCGCATTCGCTCAAACTGCTGCAGGATTTCAGGACGC
GCCACCCCGACATCCCGACCAAGTCGGGCCTGATGCTCGGGCTGGGCGAAACCGACGACGAGATCCTCGAGGTCATGCGC
GACCTGCGCGCGCACGGCGTAGACATGCTGACCCTAGGCCAGTACCTGCAGCCCTCGCGGCACCATCTGGCGGTGCTGCG
CTTCGTCACCCCCGAGCGCTTTGCGCAGTTCGAGCAGGAAGCATTGGCGATGGGTTTCCGGCACGCGGCCTGCGGGCCGA
TGGTGCGCTCGAGCTACCACGCCGACCAGCAGGCCGCCGCGGCAGCGGCAGGTTAG

Upstream 100 bases:

>100_bases
TCGGTCGCGGCGAGCGCGCTCGCACTCCACGTCAGCAAGAGGGTCAGGGCGATCCGGTGCATGGGCTAAAATTCCGTTTC
AACGCAGGTTCGAGCAGATT

Downstream 100 bases:

>100_bases
GCCGGGTCGGCCGCCGGCGCGGGGCGGCGGCGCGCCAGCGCGAGTGCTGCGCCGCCGAGCAGCACGAGTCCCCAGATCGA
AGGCTCGGGAATAGCGTGCC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MADPLQHKGAAKTSRIPIKIVPQERQRLPPWIRAKAPSLPNVGRLKGILREAKLHTVCEEASCPNLGECFGHGTATFMIL
GDLCTRRCPFCDVGHGTPLPPDADEPRHLAETIALMALKYVVITSVDRDDLRDGGAGHFAECIAAVREKSPATRIEILTP
DFRGRLDKALAALDRAPPDVMNHNLETVPRLYKAARPGADYAHSLKLLQDFRTRHPDIPTKSGLMLGLGETDDEILEVMR
DLRAHGVDMLTLGQYLQPSRHHLAVLRFVTPERFAQFEQEALAMGFRHAACGPMVRSSYHADQQAAAAAAG

Sequences:

>Translated_311_residues
MADPLQHKGAAKTSRIPIKIVPQERQRLPPWIRAKAPSLPNVGRLKGILREAKLHTVCEEASCPNLGECFGHGTATFMIL
GDLCTRRCPFCDVGHGTPLPPDADEPRHLAETIALMALKYVVITSVDRDDLRDGGAGHFAECIAAVREKSPATRIEILTP
DFRGRLDKALAALDRAPPDVMNHNLETVPRLYKAARPGADYAHSLKLLQDFRTRHPDIPTKSGLMLGLGETDDEILEVMR
DLRAHGVDMLTLGQYLQPSRHHLAVLRFVTPERFAQFEQEALAMGFRHAACGPMVRSSYHADQQAAAAAAG
>Mature_310_residues
ADPLQHKGAAKTSRIPIKIVPQERQRLPPWIRAKAPSLPNVGRLKGILREAKLHTVCEEASCPNLGECFGHGTATFMILG
DLCTRRCPFCDVGHGTPLPPDADEPRHLAETIALMALKYVVITSVDRDDLRDGGAGHFAECIAAVREKSPATRIEILTPD
FRGRLDKALAALDRAPPDVMNHNLETVPRLYKAARPGADYAHSLKLLQDFRTRHPDIPTKSGLMLGLGETDDEILEVMRD
LRAHGVDMLTLGQYLQPSRHHLAVLRFVTPERFAQFEQEALAMGFRHAACGPMVRSSYHADQQAAAAAAG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=287, Percent_Identity=48.4320557491289, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=49.3975903614458, Blast_Score=242, Evalue=3e-64,
Organism=Escherichia coli, GI1786846, Length=307, Percent_Identity=66.4495114006515, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32564533, Length=291, Percent_Identity=46.3917525773196, Blast_Score=237, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=288, Percent_Identity=45.8333333333333, Blast_Score=261, Evalue=7e-71,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=46.1805555555556, Blast_Score=271, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_THIDA (Q3SM23)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314032.1
- STRING:   Q3SM23
- GeneID:   3671416
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0274
- NMPDR:   fig|292415.3.peg.425
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TISAIRQ
- PhylomeDB:   Q3SM23
- ProtClustDB:   PRK05481
- BioCyc:   TDEN292415:TBD_0274-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34175; Mature: 34044

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPLQHKGAAKTSRIPIKIVPQERQRLPPWIRAKAPSLPNVGRLKGILREAKLHTVCEE
CCCCCCCCCCCCCCCCCEEECCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
ASCPNLGECFGHGTATFMILGDLCTRRCPFCDVGHGTPLPPDADEPRHLAETIALMALKY
CCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VVITSVDRDDLRDGGAGHFAECIAAVREKSPATRIEILTPDFRGRLDKALAALDRAPPDV
HHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHH
MNHNLETVPRLYKAARPGADYAHSLKLLQDFRTRHPDIPTKSGLMLGLGETDDEILEVMR
HCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHH
DLRAHGVDMLTLGQYLQPSRHHLAVLRFVTPERFAQFEQEALAMGFRHAACGPMVRSSYH
HHHHCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
ADQQAAAAAAG
CHHHHHHHCCC
>Mature Secondary Structure 
ADPLQHKGAAKTSRIPIKIVPQERQRLPPWIRAKAPSLPNVGRLKGILREAKLHTVCEE
CCCCCCCCCCCCCCCCEEECCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
ASCPNLGECFGHGTATFMILGDLCTRRCPFCDVGHGTPLPPDADEPRHLAETIALMALKY
CCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VVITSVDRDDLRDGGAGHFAECIAAVREKSPATRIEILTPDFRGRLDKALAALDRAPPDV
HHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHH
MNHNLETVPRLYKAARPGADYAHSLKLLQDFRTRHPDIPTKSGLMLGLGETDDEILEVMR
HCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHH
DLRAHGVDMLTLGQYLQPSRHHLAVLRFVTPERFAQFEQEALAMGFRHAACGPMVRSSYH
HHHHCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
ADQQAAAAAAG
CHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA