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The map label for this gene is oprF [H]

Identifier: 74316262

GI number: 74316262

Start: 266868

End: 267863

Strand: Direct

Name: oprF [H]

Synonym: Tbd_0244

Alternate gene names: 74316262

Gene position: 266868-267863 (Clockwise)

Preceding gene: 74316261

Following gene: 74316263

Centisome position: 9.17

GC content: 69.28

Gene sequence:

>996_bases
ATGCAGAAAAATCGACATCACCATGGCCTGTTGCTTGCCCTCGCGCTGCTCGCCGGCTGCGCGACAGTGCCCGAGAACGA
TGCGCTGACCGAGGCGCGCAGCCTTTACGACAGTGCGCGAACCGATCCCCAGGTCGTGAAACTCGCGCCGGTTGAACTGG
ACGAGGCGGGCGAGTCGCTGAGCCGCGCCGAGGCCGCCGCCTCGCAGCGCGAGAGCGGCGACGTCATCGAACAACTGGCT
TACGTGGCGAAGCAAAGGGCGGCGATCGCGCAGGAAACGGCGAAGCGCAAGGCCGCCGAGATCAGCGTCACCGAGGCGAG
CGCCGAACGCGACCGCGTGCGCCTCGAGGCGCGCACCGCCGAAGTCGCCGCTGCCCGGCAGCAGGCGACACAGGCCGAGC
TAACGGCGCAGCAGCAGGCGGAACGCGACCGCGCGCTGATCGCAGAGCGTGAGGCCGAAGTCGAGGCCGCACGGCAGCAG
GCCGCGACGGCAGAGGAGGCGGCGCGGCAGCGGGCGGATGCCCTGGAAGCAGCCCGCCTCGAGATCCAGCGCGCGCAGAC
ACGGATCGCCGAACAGGAAGAGCAGCTCCAGGCCCTGAACGCGAAGAAAACCGACCGTGGCCTCGTCGTCACGCTCGGCG
ACGTGCTGTTCGGCGTGAACAAGGCGGACCTCACGCCGGGTGGGATGCGCAACGTGCAGAAGCTCGCCGATTTCCTCAAG
CAGTACGAGACGCGCAAGCTTCTGATCGAGGGCCACACCGACAGCACTGGCGGCGACGCGCTCAATCAGGCGCTTTCCGA
ACGGCGCGCCGAGGCCGTGAAGACCGCGCTGATCGGCATGGGCGTCGACGGCGAGCGCATCGCGACGCGCGGCTACGGCA
AGGCCTTCCCGGTGGCGAGCAACAACACGGCAGCGGGCCGGCAACTCAATCGCCGGGTCGAAGTCGTGCTGTCGGAGAAC
GGCGGCGAGGTGCCGGAGCGCAAGGCCGGCGAGTGA

Upstream 100 bases:

>100_bases
TCGGCCAAGAGCCAGCGCGCCGCGGACGCCGTGCAGGAAGACATTCGTGTGTTGCGCCAGGAAATCGAGCGCAAAGCGCA
ATAAAAGTCGGGAGCCGATC

Downstream 100 bases:

>100_bases
CGGCAGGCGGGCCACGGCGCGGCGGCGATTGCGCCGCGCCGGCTCCCCGGCGACGCGTAAGCTCGGCCGGCCTTTTGTAT
TGCAGGGCTTTTCGGTAGGC

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MQKNRHHHGLLLALALLAGCATVPENDALTEARSLYDSARTDPQVVKLAPVELDEAGESLSRAEAAASQRESGDVIEQLA
YVAKQRAAIAQETAKRKAAEISVTEASAERDRVRLEARTAEVAAARQQATQAELTAQQQAERDRALIAEREAEVEAARQQ
AATAEEAARQRADALEAARLEIQRAQTRIAEQEEQLQALNAKKTDRGLVVTLGDVLFGVNKADLTPGGMRNVQKLADFLK
QYETRKLLIEGHTDSTGGDALNQALSERRAEAVKTALIGMGVDGERIATRGYGKAFPVASNNTAAGRQLNRRVEVVLSEN
GGEVPERKAGE

Sequences:

>Translated_331_residues
MQKNRHHHGLLLALALLAGCATVPENDALTEARSLYDSARTDPQVVKLAPVELDEAGESLSRAEAAASQRESGDVIEQLA
YVAKQRAAIAQETAKRKAAEISVTEASAERDRVRLEARTAEVAAARQQATQAELTAQQQAERDRALIAEREAEVEAARQQ
AATAEEAARQRADALEAARLEIQRAQTRIAEQEEQLQALNAKKTDRGLVVTLGDVLFGVNKADLTPGGMRNVQKLADFLK
QYETRKLLIEGHTDSTGGDALNQALSERRAEAVKTALIGMGVDGERIATRGYGKAFPVASNNTAAGRQLNRRVEVVLSEN
GGEVPERKAGE
>Mature_331_residues
MQKNRHHHGLLLALALLAGCATVPENDALTEARSLYDSARTDPQVVKLAPVELDEAGESLSRAEAAASQRESGDVIEQLA
YVAKQRAAIAQETAKRKAAEISVTEASAERDRVRLEARTAEVAAARQQATQAELTAQQQAERDRALIAEREAEVEAARQQ
AATAEEAARQRADALEAARLEIQRAQTRIAEQEEQLQALNAKKTDRGLVVTLGDVLFGVNKADLTPGGMRNVQKLADFLK
QYETRKLLIEGHTDSTGGDALNQALSERRAEAVKTALIGMGVDGERIATRGYGKAFPVASNNTAAGRQLNRRVEVVLSEN
GGEVPERKAGE

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=106, Percent_Identity=47.1698113207547, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788957, Length=105, Percent_Identity=38.0952380952381, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787191, Length=113, Percent_Identity=37.1681415929204, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 35715; Mature: 35715

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKNRHHHGLLLALALLAGCATVPENDALTEARSLYDSARTDPQVVKLAPVELDEAGESL
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
SRAEAAASQRESGDVIEQLAYVAKQRAAIAQETAKRKAAEISVTEASAERDRVRLEARTA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHH
EVAAARQQATQAELTAQQQAERDRALIAEREAEVEAARQQAATAEEAARQRADALEAARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQRAQTRIAEQEEQLQALNAKKTDRGLVVTLGDVLFGVNKADLTPGGMRNVQKLADFLK
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QYETRKLLIEGHTDSTGGDALNQALSERRAEAVKTALIGMGVDGERIATRGYGKAFPVAS
HHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
NNTAAGRQLNRRVEVVLSENGGEVPERKAGE
CCCHHHHHHHHHEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQKNRHHHGLLLALALLAGCATVPENDALTEARSLYDSARTDPQVVKLAPVELDEAGESL
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
SRAEAAASQRESGDVIEQLAYVAKQRAAIAQETAKRKAAEISVTEASAERDRVRLEARTA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHH
EVAAARQQATQAELTAQQQAERDRALIAEREAEVEAARQQAATAEEAARQRADALEAARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQRAQTRIAEQEEQLQALNAKKTDRGLVVTLGDVLFGVNKADLTPGGMRNVQKLADFLK
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QYETRKLLIEGHTDSTGGDALNQALSERRAEAVKTALIGMGVDGERIATRGYGKAFPVAS
HHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
NNTAAGRQLNRRVEVVLSENGGEVPERKAGE
CCCHHHHHHHHHEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]