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The map label for this gene is amtB [H]

Identifier: 74316259

GI number: 74316259

Start: 263899

End: 265305

Strand: Direct

Name: amtB [H]

Synonym: Tbd_0241

Alternate gene names: 74316259

Gene position: 263899-265305 (Clockwise)

Preceding gene: 74316258

Following gene: 74316261

Centisome position: 9.07

GC content: 68.59

Gene sequence:

>1407_bases
ATGAAAAAACTCTTTGCTTCGTTCGGCCTGATCCTCGCGCTGCTGGCGCCGGGCCTCGCGGCCGCGCAGGACGCGGCCGT
GCCGATGGATGGCGCCGCAGCAGTCGAAACGGCGCCGGCGGCTGTAGCGCCGGCAGCCGCAGTTCCGGCCGAAACAGCCG
CGACGCCGGTCCCAGACAAGGGCGACACGACCTGGATGATGGTCTCGACGTTGCTCGTCGTCCTGATGATCGTCCCGGGC
GTCGCACTCTTCTACGGCGGGCTCGTGCGCGCCAAGAACATGCTGTCGGTGCTGACGCAGGTGATGGCGATCTTCTGCCT
GATATCGGTGCTGTGGGCGGTCTATGGCTACTCGCTGGCGTTCGGCGACGGCGGCGGCATGAACTGGGCGATCGGCGACC
TCTCCAAGCTCTTCCTCGCCGGGATCACGCCCGACAGCACCGCCGCGACCTTCTCCGACGGCGTCGTCATTCCCGAATTC
ACCTTCGTCGCCTTCCAGCTGACCTTCGCCGCGATCACGGTCGCACTGATCGTCGGCGGGCTCGCCGAGCGCGTGAAATT
CTCGGCGCTGCTCGTGTTCGCGGTGCTGTGGTTCACCTTCTCCTACCTGCCGATCGCGCACATGGTGTGGGCGACCGGCG
GCTATCTGTTCGAGAAGGGCGACCTCGACTTCGCGGGCGGGACCGTGGTCCACATCAACGCGGGCGTCGCTGCGCTCGTC
GGCGCGATCGTACTCGGCAAGCGCATCGGCTACGGCCGCGACGCGATGCCGCCGCACAACCTGCCGATGACGATGATCGG
CGCGTCGCTGCTGTGGGTCGGCTGGTTCGGCTTCAACGCCGGCTCCAACCTCGAAGCGACCGGCGGCGCGGCGCTCGCCT
TCATCAACACGATACTCGCGACCGCCGCGGCAGGGCTGGCGTGGATGTTCGCCGAGTGGATGGTGCGCGGCAAGCCTTCG
TTGCTCGGCGTCGCGTCGGGCGTGGTTGCCGGGCTCGTCGCGATCACGCCCGCAGCGGGCCTGGTCGGGCCGATGGGCGG
GATCGTGCTCGGCGCGGTCGCCGGCGTCGTCTGCCTGTGGGGCGTCAGCGGGCTCAAGTCGGCGCTCGGCTACGACGACA
GCCTCGACGTCTTCGGCATCCACGGCATCGGCGGCATCATCGGCGCGATCGGCACCGGCATCTTCGTCTCGCCGACGCTC
GGCGGCGTCGGGGTCGACGGCTACACGCTCGCCGGCCAGGTCATGACACAGGCGAGCGGCGTGCTGATCACGATCGTGTG
GTCGGCGGTCGTCAGCTTCGTCGCCTTCAAGCTCATCGACGTGACCCTCGGCTTGCGCGTCGCCGAAGAACAGGAGCGCG
AGGGACTCGACACCGCGTCGCACGGCGAGCGCGCGTACTCCGTCTAG

Upstream 100 bases:

>100_bases
GGCAAGATCGGCGACGGCAAGATCTTCGTCAGCAGCCTGGAACAGGTCGTGCGCATCCGTACCGGCGAGTCCGGCCCGGA
CGCACTGTGAGGAGCCGGAC

Downstream 100 bases:

>100_bases
CCGTTCTTTCTCCTGCTGGAGGTCTGGGGCGCTCTGCAGGGCGCCCCTTTTTATTGAGTCGGGCAGCGGACGGCGGCACG
GCCGAAGCGTGGACGTGCGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKKLFASFGLILALLAPGLAAAQDAAVPMDGAAAVETAPAAVAPAAAVPAETAATPVPDKGDTTWMMVSTLLVVLMIVPG
VALFYGGLVRAKNMLSVLTQVMAIFCLISVLWAVYGYSLAFGDGGGMNWAIGDLSKLFLAGITPDSTAATFSDGVVIPEF
TFVAFQLTFAAITVALIVGGLAERVKFSALLVFAVLWFTFSYLPIAHMVWATGGYLFEKGDLDFAGGTVVHINAGVAALV
GAIVLGKRIGYGRDAMPPHNLPMTMIGASLLWVGWFGFNAGSNLEATGGAALAFINTILATAAAGLAWMFAEWMVRGKPS
LLGVASGVVAGLVAITPAAGLVGPMGGIVLGAVAGVVCLWGVSGLKSALGYDDSLDVFGIHGIGGIIGAIGTGIFVSPTL
GGVGVDGYTLAGQVMTQASGVLITIVWSAVVSFVAFKLIDVTLGLRVAEEQEREGLDTASHGERAYSV

Sequences:

>Translated_468_residues
MKKLFASFGLILALLAPGLAAAQDAAVPMDGAAAVETAPAAVAPAAAVPAETAATPVPDKGDTTWMMVSTLLVVLMIVPG
VALFYGGLVRAKNMLSVLTQVMAIFCLISVLWAVYGYSLAFGDGGGMNWAIGDLSKLFLAGITPDSTAATFSDGVVIPEF
TFVAFQLTFAAITVALIVGGLAERVKFSALLVFAVLWFTFSYLPIAHMVWATGGYLFEKGDLDFAGGTVVHINAGVAALV
GAIVLGKRIGYGRDAMPPHNLPMTMIGASLLWVGWFGFNAGSNLEATGGAALAFINTILATAAAGLAWMFAEWMVRGKPS
LLGVASGVVAGLVAITPAAGLVGPMGGIVLGAVAGVVCLWGVSGLKSALGYDDSLDVFGIHGIGGIIGAIGTGIFVSPTL
GGVGVDGYTLAGQVMTQASGVLITIVWSAVVSFVAFKLIDVTLGLRVAEEQEREGLDTASHGERAYSV
>Mature_468_residues
MKKLFASFGLILALLAPGLAAAQDAAVPMDGAAAVETAPAAVAPAAAVPAETAATPVPDKGDTTWMMVSTLLVVLMIVPG
VALFYGGLVRAKNMLSVLTQVMAIFCLISVLWAVYGYSLAFGDGGGMNWAIGDLSKLFLAGITPDSTAATFSDGVVIPEF
TFVAFQLTFAAITVALIVGGLAERVKFSALLVFAVLWFTFSYLPIAHMVWATGGYLFEKGDLDFAGGTVVHINAGVAALV
GAIVLGKRIGYGRDAMPPHNLPMTMIGASLLWVGWFGFNAGSNLEATGGAALAFINTILATAAAGLAWMFAEWMVRGKPS
LLGVASGVVAGLVAITPAAGLVGPMGGIVLGAVAGVVCLWGVSGLKSALGYDDSLDVFGIHGIGGIIGAIGTGIFVSPTL
GGVGVDGYTLAGQVMTQASGVLITIVWSAVVSFVAFKLIDVTLGLRVAEEQEREGLDTASHGERAYSV

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=435, Percent_Identity=54.9425287356322, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17550180, Length=441, Percent_Identity=31.5192743764172, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=30.1587301587302, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32564042, Length=442, Percent_Identity=28.0542986425339, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI133931100, Length=430, Percent_Identity=24.8837209302326, Blast_Score=108, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6324187, Length=415, Percent_Identity=37.3493975903614, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=34.192037470726, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321559, Length=407, Percent_Identity=32.1867321867322, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI161078314, Length=424, Percent_Identity=27.5943396226415, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47710; Mature: 47710

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFASFGLILALLAPGLAAAQDAAVPMDGAAAVETAPAAVAPAAAVPAETAATPVPDK
CHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHCCCCHHCCHHCCCCCCCCCCCCCC
GDTTWMMVSTLLVVLMIVPGVALFYGGLVRAKNMLSVLTQVMAIFCLISVLWAVYGYSLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
FGDGGGMNWAIGDLSKLFLAGITPDSTAATFSDGVVIPEFTFVAFQLTFAAITVALIVGG
ECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
LAERVKFSALLVFAVLWFTFSYLPIAHMVWATGGYLFEKGDLDFAGGTVVHINAGVAALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCHHHHH
GAIVLGKRIGYGRDAMPPHNLPMTMIGASLLWVGWFGFNAGSNLEATGGAALAFINTILA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
TAAAGLAWMFAEWMVRGKPSLLGVASGVVAGLVAITPAAGLVGPMGGIVLGAVAGVVCLW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHH
GVSGLKSALGYDDSLDVFGIHGIGGIIGAIGTGIFVSPTLGGVGVDGYTLAGQVMTQASG
HHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHCCC
VLITIVWSAVVSFVAFKLIDVTLGLRVAEEQEREGLDTASHGERAYSV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKLFASFGLILALLAPGLAAAQDAAVPMDGAAAVETAPAAVAPAAAVPAETAATPVPDK
CHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHCCCCHHCCHHCCCCCCCCCCCCCC
GDTTWMMVSTLLVVLMIVPGVALFYGGLVRAKNMLSVLTQVMAIFCLISVLWAVYGYSLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
FGDGGGMNWAIGDLSKLFLAGITPDSTAATFSDGVVIPEFTFVAFQLTFAAITVALIVGG
ECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
LAERVKFSALLVFAVLWFTFSYLPIAHMVWATGGYLFEKGDLDFAGGTVVHINAGVAALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCHHHHH
GAIVLGKRIGYGRDAMPPHNLPMTMIGASLLWVGWFGFNAGSNLEATGGAALAFINTILA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
TAAAGLAWMFAEWMVRGKPSLLGVASGVVAGLVAITPAAGLVGPMGGIVLGAVAGVVCLW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHH
GVSGLKSALGYDDSLDVFGIHGIGGIIGAIGTGIFVSPTLGGVGVDGYTLAGQVMTQASG
HHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHCCC
VLITIVWSAVVSFVAFKLIDVTLGLRVAEEQEREGLDTASHGERAYSV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]