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The map label for this gene is phoR [H]

Identifier: 74316235

GI number: 74316235

Start: 237503

End: 238852

Strand: Direct

Name: phoR [H]

Synonym: Tbd_0217

Alternate gene names: 74316235

Gene position: 237503-238852 (Clockwise)

Preceding gene: 74316234

Following gene: 74316240

Centisome position: 8.16

GC content: 67.26

Gene sequence:

>1350_bases
ATGATGGGTTTCTGGTGGCGACCGCTCAGCGTATTGGCCAGCGTGATGCTGGTCGCGCTGATCCTGTGGGCGGGGTCGGG
CTGGGTCGCGGCGCTCGGCTTTCTCGCCGGCATCCAGGCCTTCGTCATGCTGCGCCGCCTGTGGCAGGAATACCGTCTGT
CGCGCTGGCTCGAAAACCCGGACGAGGTCGATCCGCCCGACGCGACCGGTACCTGGGGCGACATCTTCTACCGCCTGCAG
AAACTCCAGCGCCGCCAGCGAGCGAGCCGCAGCGAGCTCACCAATGCGCTCGAACAGTTCGAACACGCCGCGCAGGCGGT
GCCCGACGCGATGGTCATCCTCAACGGCACCGAGCAGATCGAATGGTGCAACCCGGCGTCGCGCAGCTACCTGGGGCTCG
ATTGCGAGCGCGACCAGGGCCAGTTCGTCCGTTACCTGATGCGGCAGGCGCATTTTCTCGACTTTCTCGACGCCGCCGAC
TATTCGCGCCGCCTCGTCTGCAAGTCGCCGGTCAACCGCGAGCTCACGCTCTCGGTCCAGCTCGTTCCCTTCGGCGAAGG
CAAGAAGCTGCTGGTCGCGCGCGATATCACCGACCTCGAGCGGGTCGACGCGATGCGGCGCGACTTCATCGCCAACGTCT
CGCACGAACTGCGCACGCCCCTGACCGTCGTCGGCGGCTTCGTCGAAACGCTCGCCGAGACAGACGATCTGCCGCCCGCC
GAGAGCCGCCGCTATTTCGACCTCATGCTCGACCACACGCGGCGCATGCAGCGGCTGCTGGACGATTTGTTGATACTCTC
GCGGCTCGAAAGCGCCGATCACAGCCTGAGCGACGAGCCCGTCAACGTGCCGGAGATGGTGCAGGCGCTGAAGTCCGAGG
CCGAGTCGCTGAGCCGGGGACGGCATCGCGTCAGCGTGCAGGCCACGAGTGGCACGTGGCTCAAGGGCAGCCAGCAGGAA
GTCCGCAGCGCATTCGGCAACCTGGTCTCCAACGCGGTGCGTTACACGCCTGAGGGCGGCGCAATCACCCTCGGTTGGCA
CGAGCGCGACGGCGAGGCGGTTTTTTCCGTGAAGGACACCGGGGAAGGCATCGCCGCCGAGCACATTCCGCGGCTGACCG
AGCGTTTCTACCGGGTCGACCGCAGCCGCTCGCGCGAGACCGGCGGCACCGGCCTTGGCCTTGCGATCGTCAAGCACGTG
CTGACCCGTCACCACGCGCGTCTCGACATCCAGTCGACACCCGGGCGCGGTAGCGCCTTCTCCGCGATCTTCCCGGCTCA
GCGCGTCCTCGCGGCGCCCGAGAACGCCCAAGTCATTCCGTTTCCTGCGCGCGACCAAGGCGCCGCCTGA

Upstream 100 bases:

>100_bases
CCCTCCGGGCACGCCGCGCTCGTGCAGACGGTACGCGGCTCCGGCTATCGCTTTTCGACCGAAGTGGGCTGATAATCGCC
CCTGTTTCCTTACGGCGATA

Downstream 100 bases:

>100_bases
GCGCCGCGCCAAGCCGCGCGTCCAGGTCGGGCGCTGCGGCGCGCGGCGTATCCCGTTGCCTCACGCCCCACACCGCATTC
GGGAAATGCGGGTCGTCGGT

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MMGFWWRPLSVLASVMLVALILWAGSGWVAALGFLAGIQAFVMLRRLWQEYRLSRWLENPDEVDPPDATGTWGDIFYRLQ
KLQRRQRASRSELTNALEQFEHAAQAVPDAMVILNGTEQIEWCNPASRSYLGLDCERDQGQFVRYLMRQAHFLDFLDAAD
YSRRLVCKSPVNRELTLSVQLVPFGEGKKLLVARDITDLERVDAMRRDFIANVSHELRTPLTVVGGFVETLAETDDLPPA
ESRRYFDLMLDHTRRMQRLLDDLLILSRLESADHSLSDEPVNVPEMVQALKSEAESLSRGRHRVSVQATSGTWLKGSQQE
VRSAFGNLVSNAVRYTPEGGAITLGWHERDGEAVFSVKDTGEGIAAEHIPRLTERFYRVDRSRSRETGGTGLGLAIVKHV
LTRHHARLDIQSTPGRGSAFSAIFPAQRVLAAPENAQVIPFPARDQGAA

Sequences:

>Translated_449_residues
MMGFWWRPLSVLASVMLVALILWAGSGWVAALGFLAGIQAFVMLRRLWQEYRLSRWLENPDEVDPPDATGTWGDIFYRLQ
KLQRRQRASRSELTNALEQFEHAAQAVPDAMVILNGTEQIEWCNPASRSYLGLDCERDQGQFVRYLMRQAHFLDFLDAAD
YSRRLVCKSPVNRELTLSVQLVPFGEGKKLLVARDITDLERVDAMRRDFIANVSHELRTPLTVVGGFVETLAETDDLPPA
ESRRYFDLMLDHTRRMQRLLDDLLILSRLESADHSLSDEPVNVPEMVQALKSEAESLSRGRHRVSVQATSGTWLKGSQQE
VRSAFGNLVSNAVRYTPEGGAITLGWHERDGEAVFSVKDTGEGIAAEHIPRLTERFYRVDRSRSRETGGTGLGLAIVKHV
LTRHHARLDIQSTPGRGSAFSAIFPAQRVLAAPENAQVIPFPARDQGAA
>Mature_449_residues
MMGFWWRPLSVLASVMLVALILWAGSGWVAALGFLAGIQAFVMLRRLWQEYRLSRWLENPDEVDPPDATGTWGDIFYRLQ
KLQRRQRASRSELTNALEQFEHAAQAVPDAMVILNGTEQIEWCNPASRSYLGLDCERDQGQFVRYLMRQAHFLDFLDAAD
YSRRLVCKSPVNRELTLSVQLVPFGEGKKLLVARDITDLERVDAMRRDFIANVSHELRTPLTVVGGFVETLAETDDLPPA
ESRRYFDLMLDHTRRMQRLLDDLLILSRLESADHSLSDEPVNVPEMVQALKSEAESLSRGRHRVSVQATSGTWLKGSQQE
VRSAFGNLVSNAVRYTPEGGAITLGWHERDGEAVFSVKDTGEGIAAEHIPRLTERFYRVDRSRSRETGGTGLGLAIVKHV
LTRHHARLDIQSTPGRGSAFSAIFPAQRVLAAPENAQVIPFPARDQGAA

Specific function: Member of the two-component regulatory system phoR/phoB involved in the phosphate regulon genes expression. PhoR may function as a membrane-associated protein kinase that phosphorylates phoB in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=413, Percent_Identity=40.9200968523002, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1788393, Length=259, Percent_Identity=28.1853281853282, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786783, Length=268, Percent_Identity=30.5970149253731, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI145693157, Length=340, Percent_Identity=25, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790346, Length=229, Percent_Identity=30.5676855895196, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788713, Length=253, Percent_Identity=26.0869565217391, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI48994928, Length=304, Percent_Identity=26.6447368421053, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790861, Length=193, Percent_Identity=31.6062176165803, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789149, Length=226, Percent_Identity=29.2035398230088, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081816, Length=242, Percent_Identity=30.5785123966942, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790551, Length=209, Percent_Identity=32.0574162679426, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789403, Length=201, Percent_Identity=30.3482587064677, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=26.2008733624454, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787894, Length=225, Percent_Identity=27.1111111111111, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788549, Length=388, Percent_Identity=22.1649484536082, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=24.8908296943231, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789808, Length=227, Percent_Identity=26.431718061674, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788279, Length=226, Percent_Identity=24.3362831858407, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR021766
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR014310 [H]

Pfam domain/function: PF11808 DUF3329; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50445; Mature: 50445

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGFWWRPLSVLASVMLVALILWAGSGWVAALGFLAGIQAFVMLRRLWQEYRLSRWLENP
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DEVDPPDATGTWGDIFYRLQKLQRRQRASRSELTNALEQFEHAAQAVPDAMVILNGTEQI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EWCNPASRSYLGLDCERDQGQFVRYLMRQAHFLDFLDAADYSRRLVCKSPVNRELTLSVQ
CCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHCEEEECCCCCCEEEEEEE
LVPFGEGKKLLVARDITDLERVDAMRRDFIANVSHELRTPLTVVGGFVETLAETDDLPPA
EEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCC
ESRRYFDLMLDHTRRMQRLLDDLLILSRLESADHSLSDEPVNVPEMVQALKSEAESLSRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RHRVSVQATSGTWLKGSQQEVRSAFGNLVSNAVRYTPEGGAITLGWHERDGEAVFSVKDT
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCEEEEECCC
GEGIAAEHIPRLTERFYRVDRSRSRETGGTGLGLAIVKHVLTRHHARLDIQSTPGRGSAF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCH
SAIFPAQRVLAAPENAQVIPFPARDQGAA
HHHHHHHHHHCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MMGFWWRPLSVLASVMLVALILWAGSGWVAALGFLAGIQAFVMLRRLWQEYRLSRWLENP
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DEVDPPDATGTWGDIFYRLQKLQRRQRASRSELTNALEQFEHAAQAVPDAMVILNGTEQI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EWCNPASRSYLGLDCERDQGQFVRYLMRQAHFLDFLDAADYSRRLVCKSPVNRELTLSVQ
CCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHCEEEECCCCCCEEEEEEE
LVPFGEGKKLLVARDITDLERVDAMRRDFIANVSHELRTPLTVVGGFVETLAETDDLPPA
EEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCC
ESRRYFDLMLDHTRRMQRLLDDLLILSRLESADHSLSDEPVNVPEMVQALKSEAESLSRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RHRVSVQATSGTWLKGSQQEVRSAFGNLVSNAVRYTPEGGAITLGWHERDGEAVFSVKDT
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCEEEEECCC
GEGIAAEHIPRLTERFYRVDRSRSRETGGTGLGLAIVKHVLTRHHARLDIQSTPGRGSAF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCH
SAIFPAQRVLAAPENAQVIPFPARDQGAA
HHHHHHHHHHCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043; 2115874 [H]