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The map label for this gene is phoB [H]

Identifier: 74316234

GI number: 74316234

Start: 236782

End: 237474

Strand: Direct

Name: phoB [H]

Synonym: Tbd_0216

Alternate gene names: 74316234

Gene position: 236782-237474 (Clockwise)

Preceding gene: 74316232

Following gene: 74316235

Centisome position: 8.14

GC content: 67.1

Gene sequence:

>693_bases
ATGGCTGCCAATATTCTGCTGGTCGAGGACGAACCCGGGATCCAGGAGCTGCTGAAATTCAATCTCACGCAGGCGGGTCA
TCAGGTCACCGCGGCGGGGGATGCCGAGCACGCGCTGAAATTCCTCAAGACGACGCTACCCGACGTCATCCTGCTCGACT
GGATGTTGCCCGGCATGTCCGGCATCGACCTGTGCCGGCGCCTGCGCGGCGACGCGCGCTATCAGCCGGTTCCGATCATC
ATGCTGACCGCGCGCGGCGAGGAGCGCGACAAGGTGCTCGGCCTCGACACCGGCGCCGACGACTACATCACCAAGCCGTT
CTCGCCGCGCGAACTGGTCTCGCGGATCAAGGCCGTGTTGCGCCGCCGCGCGCCGCAGATGACCGAGGAAGCGGTCGAAG
TCGCCGGCCTGCGGCTCGACCCGGTAAGCCATCGCGTCCAGGGCAAGGGCATCGCGCTCGAGCTCGGTCCGACCGAGTTC
CGCCTGCTGCATTTCTTCATGACCCATCCCGAGCGCGTCTACACGCGGCCGCAGCTGCTCGATCAGGTCTGGGGCGACGA
CGTGTTCGTCGAAGAGCGCACGGTCGACGTCCACATCCGTCGCCTGCGGCTCGCGCTCGAGCCCTCCGGGCACGCCGCGC
TCGTGCAGACGGTACGCGGCTCCGGCTATCGCTTTTCGACCGAAGTGGGCTGA

Upstream 100 bases:

>100_bases
ACCAGCGCGGGCGCGGGAACGCCCCGCCGCGGCGCGTGACGTCATAAATCAGTCATACTGCCGTCATAGCATGGCCACCA
TTCAAAATGCTGGAGCGACG

Downstream 100 bases:

>100_bases
TAATCGCCCCTGTTTCCTTACGGCGATAATGATGGGTTTCTGGTGGCGACCGCTCAGCGTATTGGCCAGCGTGATGCTGG
TCGCGCTGATCCTGTGGGCG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MAANILLVEDEPGIQELLKFNLTQAGHQVTAAGDAEHALKFLKTTLPDVILLDWMLPGMSGIDLCRRLRGDARYQPVPII
MLTARGEERDKVLGLDTGADDYITKPFSPRELVSRIKAVLRRRAPQMTEEAVEVAGLRLDPVSHRVQGKGIALELGPTEF
RLLHFFMTHPERVYTRPQLLDQVWGDDVFVEERTVDVHIRRLRLALEPSGHAALVQTVRGSGYRFSTEVG

Sequences:

>Translated_230_residues
MAANILLVEDEPGIQELLKFNLTQAGHQVTAAGDAEHALKFLKTTLPDVILLDWMLPGMSGIDLCRRLRGDARYQPVPII
MLTARGEERDKVLGLDTGADDYITKPFSPRELVSRIKAVLRRRAPQMTEEAVEVAGLRLDPVSHRVQGKGIALELGPTEF
RLLHFFMTHPERVYTRPQLLDQVWGDDVFVEERTVDVHIRRLRLALEPSGHAALVQTVRGSGYRFSTEVG
>Mature_229_residues
AANILLVEDEPGIQELLKFNLTQAGHQVTAAGDAEHALKFLKTTLPDVILLDWMLPGMSGIDLCRRLRGDARYQPVPIIM
LTARGEERDKVLGLDTGADDYITKPFSPRELVSRIKAVLRRRAPQMTEEAVEVAGLRLDPVSHRVQGKGIALELGPTEFR
LLHFFMTHPERVYTRPQLLDQVWGDDVFVEERTVDVHIRRLRLALEPSGHAALVQTVRGSGYRFSTEVG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=62.5550660792952, Blast_Score=290, Evalue=4e-80,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=41.8502202643172, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=38.8646288209607, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786784, Length=219, Percent_Identity=38.8127853881278, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=37.5565610859729, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=39.1891891891892, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1790863, Length=235, Percent_Identity=35.3191489361702, Blast_Score=126, Evalue=9e-31,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=36.4444444444444, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=39.9122807017544, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=32.1266968325792, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1787229, Length=236, Percent_Identity=35.1694915254237, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1789402, Length=219, Percent_Identity=33.7899543378995, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1790552, Length=219, Percent_Identity=33.7899543378995, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=30.3418803418803, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=28.7878787878788, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=25.2100840336134, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1788521, Length=209, Percent_Identity=30.622009569378, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25810; Mature: 25679

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANILLVEDEPGIQELLKFNLTQAGHQVTAAGDAEHALKFLKTTLPDVILLDWMLPGMS
CCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCC
GIDLCRRLRGDARYQPVPIIMLTARGEERDKVLGLDTGADDYITKPFSPRELVSRIKAVL
HHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RRRAPQMTEEAVEVAGLRLDPVSHRVQGKGIALELGPTEFRLLHFFMTHPERVYTRPQLL
HHCCCCHHHHHHHHHCCEECCHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHHHCCHHHH
DQVWGDDVFVEERTVDVHIRRLRLALEPSGHAALVQTVRGSGYRFSTEVG
HHHCCCCEEEECCHHHHEEEEEEEEECCCCCHHHHHHHCCCCEEEECCCC
>Mature Secondary Structure 
AANILLVEDEPGIQELLKFNLTQAGHQVTAAGDAEHALKFLKTTLPDVILLDWMLPGMS
CCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCC
GIDLCRRLRGDARYQPVPIIMLTARGEERDKVLGLDTGADDYITKPFSPRELVSRIKAVL
HHHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RRRAPQMTEEAVEVAGLRLDPVSHRVQGKGIALELGPTEFRLLHFFMTHPERVYTRPQLL
HHCCCCHHHHHHHHHCCEECCHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHHHCCHHHH
DQVWGDDVFVEERTVDVHIRRLRLALEPSGHAALVQTVRGSGYRFSTEVG
HHHCCCCEEEECCHHHHEEEEEEEEECCCCCHHHHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]