Click here to switch to the map view.

The map label for this gene is rep [H]

Identifier: 74316184

GI number: 74316184

Start: 179706

End: 181688

Strand: Reverse

Name: rep [H]

Synonym: Tbd_0166

Alternate gene names: 74316184

Gene position: 181688-179706 (Counterclockwise)

Preceding gene: 74316185

Following gene: 74316183

Centisome position: 6.24

GC content: 65.56

Gene sequence:

>1983_bases
ATGAGCCTGCTCGCAGCGCTCAACCCGCCGCAGCGCGAAGCGGCGAAATACCTCGACGGCCCGCTCCTCGTGCTGGCCGG
GGCGGGGTCGGGCAAGACCCGCGTCATCACGCACAAGATCGCCTACCTGATCGGCGAGTGCGGCTACAAGCCGGGCTCGA
TCGCCGCGATCACCTTCACCAACAAGGCCGCGCGCGAGATGGCCGAGCGCGCCGCCAAGCTGACCCACGGGGTCAACACG
AAGGGCCTGATCGTCACGACCTTCCACTCGATGGGCCTCAGAATGCTGCGCGAGGACGCGAAGTTCGCCGAGCTCAAGCC
CGCATTCTCGATCCTCGATTCGGCCGACGCCGCCGGCATCGTGTCGGAGATCATCAAGACCACCGACAAGCAGGAAATCC
GCCGCGCGGTGTCGCGCATCTCGTTGTGGAAGAACGAGCTCAAGTCGCCCGCCGCCGCCCTGGCTTCGGCTGAGGACGAC
AACGCGCGCGTGTACGCAAAGATCTACGACCGCTACGACGCGACGCTGCGCGCCTATCAGGCGGTCGACTTCGACGACCT
GATCCGCCTGCCGGCCGAACTGCTCGACACCCACGCCGAGCTGCGCGAGAAGTGGCAGAACCGCCTGCGCCACATCCTCG
TCGACGAATACCAGGACACCAACGTCGCGCAGTACCGGCTACTGCAGAAGCTCACCGGCGTGCGCGCCGCGTTCACCGCG
GTCGGCGACGACGACCAGGCGATCTACGGCTGGCGCGGCGCCTCGGCCGACAACCTCAGGCAGCTCAACGAGGACTACCC
GCATCTGCATGTCGTCAAGCTCGAGCAGAACTACCGCTCGACCGTACGCATCCTCAAGGCCGCCAACAGCCTCATCGCCA
ACAACCCCAAGCTCTTCGAAAAGCGCCTGTGGAGCGACCTCGGCCACGGCGACGCGATCCAGGTCATGCCGACCAAGGAC
GACGAGCACGAGGCCGAATCGGTCGTCATGCGCCTGCTATCGCACAAGTTCCAGCACCGCACCGAGTGGCGCGACTACGC
GATCCTCTACCGCGGCAACTACCAGGCGCGCGTGTTCGAGCAGGCGCTCCGGAACGAGAAGGTGCCCTATCAGCTCTCGG
GCGGCCAGTCGTTCTTCGACAAGGCGGAAATCAAGGACGTGATCGCCTACCTGCGCCTGATCGCCAACAGCGACGACGAC
CCTGCCTTCATTCGCGCCGTCACCACGCCCAAGCGCGGCATCGGCGCCGCCACGCTCGAAAAGCTCGGGCAGGTCGCCGC
GACCCTGCACGTCAGCCTGTTCGAGGCAGTCTTTTCGGCCGCCGCCGCATCACAGATCGGTGAGCGACATCTCACGCCAC
TCACCGAGTTCTGCAACTTCATCAACCGCATGGAGGAGCGCGTCGCGCGCGAGCCGGCCGGCGAACTGCTCGACGACCTC
CTGAAGACGATCGGCTACGAGGTCTATCTCTTCGACCAGGACGACCAGCGCGCGGCGCAGAGCAAATGGAACAACGTACT
CGAATTCGCCGCCTGGATTACGAAAAAAGGCGAAGAGGACGAGAAGAACCTGCTGCAGCTGACGCAAACCATCGCGCTGA
TCACGCTGCTCGAAGGCCGCGAAGAGGAAGAGTCCGACGCCGTACGCCTGTCGACCCTGCACGCAGCCAAGGGACTCGAA
TTCGGCCACGTCTTCCTGGTCGGCGTCGAGGAGAACATCCTGCCGCACCGCGACGCGGTCGACGAAGGCCGCCTCGAAGA
GGAGCGCCGCCTGATGTACGTCGGCGTCACCCGCGCGCGCAAGTCGCTGACGCTGTCCTACTGCTCGCGCCGCAAGCGCG
CCCGCGAGTCGGTCGCCTGCGATCCGTCACGCTTCATCGATGAATTGGGCGATGAGGTGCGCATGCCGGAAAAACCGACG
ACCGCGGAAGCGAAGGCCAGCGGCAGCGACCGGCTGGCGGCGTTGAAGGCGATGCTGGGCTGA

Upstream 100 bases:

>100_bases
AGGTGCCGTGCCTGCGGATCAGGGACGACGACGGGCGCGAGACCTGGCTTTACGAATCCGACGCCATCAACGCCTGGCTC
GAGCGCGAATTCGGCACGGC

Downstream 100 bases:

>100_bases
GGTCTACCCATCGGAAAGTTCCGCCGCGCACCGCCCGAGGGGTGCGACCAAAAGTCCGTCATCGATTCAGAGCGGCCCTT
CACGGCGTATCCGCTGAGTG

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MSLLAALNPPQREAAKYLDGPLLVLAGAGSGKTRVITHKIAYLIGECGYKPGSIAAITFTNKAAREMAERAAKLTHGVNT
KGLIVTTFHSMGLRMLREDAKFAELKPAFSILDSADAAGIVSEIIKTTDKQEIRRAVSRISLWKNELKSPAAALASAEDD
NARVYAKIYDRYDATLRAYQAVDFDDLIRLPAELLDTHAELREKWQNRLRHILVDEYQDTNVAQYRLLQKLTGVRAAFTA
VGDDDQAIYGWRGASADNLRQLNEDYPHLHVVKLEQNYRSTVRILKAANSLIANNPKLFEKRLWSDLGHGDAIQVMPTKD
DEHEAESVVMRLLSHKFQHRTEWRDYAILYRGNYQARVFEQALRNEKVPYQLSGGQSFFDKAEIKDVIAYLRLIANSDDD
PAFIRAVTTPKRGIGAATLEKLGQVAATLHVSLFEAVFSAAAASQIGERHLTPLTEFCNFINRMEERVAREPAGELLDDL
LKTIGYEVYLFDQDDQRAAQSKWNNVLEFAAWITKKGEEDEKNLLQLTQTIALITLLEGREEEESDAVRLSTLHAAKGLE
FGHVFLVGVEENILPHRDAVDEGRLEEERRLMYVGVTRARKSLTLSYCSRRKRARESVACDPSRFIDELGDEVRMPEKPT
TAEAKASGSDRLAALKAMLG

Sequences:

>Translated_660_residues
MSLLAALNPPQREAAKYLDGPLLVLAGAGSGKTRVITHKIAYLIGECGYKPGSIAAITFTNKAAREMAERAAKLTHGVNT
KGLIVTTFHSMGLRMLREDAKFAELKPAFSILDSADAAGIVSEIIKTTDKQEIRRAVSRISLWKNELKSPAAALASAEDD
NARVYAKIYDRYDATLRAYQAVDFDDLIRLPAELLDTHAELREKWQNRLRHILVDEYQDTNVAQYRLLQKLTGVRAAFTA
VGDDDQAIYGWRGASADNLRQLNEDYPHLHVVKLEQNYRSTVRILKAANSLIANNPKLFEKRLWSDLGHGDAIQVMPTKD
DEHEAESVVMRLLSHKFQHRTEWRDYAILYRGNYQARVFEQALRNEKVPYQLSGGQSFFDKAEIKDVIAYLRLIANSDDD
PAFIRAVTTPKRGIGAATLEKLGQVAATLHVSLFEAVFSAAAASQIGERHLTPLTEFCNFINRMEERVAREPAGELLDDL
LKTIGYEVYLFDQDDQRAAQSKWNNVLEFAAWITKKGEEDEKNLLQLTQTIALITLLEGREEEESDAVRLSTLHAAKGLE
FGHVFLVGVEENILPHRDAVDEGRLEEERRLMYVGVTRARKSLTLSYCSRRKRARESVACDPSRFIDELGDEVRMPEKPT
TAEAKASGSDRLAALKAMLG
>Mature_659_residues
SLLAALNPPQREAAKYLDGPLLVLAGAGSGKTRVITHKIAYLIGECGYKPGSIAAITFTNKAAREMAERAAKLTHGVNTK
GLIVTTFHSMGLRMLREDAKFAELKPAFSILDSADAAGIVSEIIKTTDKQEIRRAVSRISLWKNELKSPAAALASAEDDN
ARVYAKIYDRYDATLRAYQAVDFDDLIRLPAELLDTHAELREKWQNRLRHILVDEYQDTNVAQYRLLQKLTGVRAAFTAV
GDDDQAIYGWRGASADNLRQLNEDYPHLHVVKLEQNYRSTVRILKAANSLIANNPKLFEKRLWSDLGHGDAIQVMPTKDD
EHEAESVVMRLLSHKFQHRTEWRDYAILYRGNYQARVFEQALRNEKVPYQLSGGQSFFDKAEIKDVIAYLRLIANSDDDP
AFIRAVTTPKRGIGAATLEKLGQVAATLHVSLFEAVFSAAAASQIGERHLTPLTEFCNFINRMEERVAREPAGELLDDLL
KTIGYEVYLFDQDDQRAAQSKWNNVLEFAAWITKKGEEDEKNLLQLTQTIALITLLEGREEEESDAVRLSTLHAAKGLEF
GHVFLVGVEENILPHRDAVDEGRLEEERRLMYVGVTRARKSLTLSYCSRRKRARESVACDPSRFIDELGDEVRMPEKPTT
AEAKASGSDRLAALKAMLG

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=666, Percent_Identity=47.7477477477478, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI2367296, Length=647, Percent_Identity=39.8763523956723, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1787196, Length=373, Percent_Identity=28.686327077748, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=479, Percent_Identity=32.1503131524008, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6324477, Length=660, Percent_Identity=21.6666666666667, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 74057; Mature: 73926

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLAALNPPQREAAKYLDGPLLVLAGAGSGKTRVITHKIAYLIGECGYKPGSIAAITFT
CCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEC
NKAAREMAERAAKLTHGVNTKGLIVTTFHSMGLRMLREDAKFAELKPAFSILDSADAAGI
CHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHH
VSEIIKTTDKQEIRRAVSRISLWKNELKSPAAALASAEDDNARVYAKIYDRYDATLRAYQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHH
AVDFDDLIRLPAELLDTHAELREKWQNRLRHILVDEYQDTNVAQYRLLQKLTGVRAAFTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
VGDDDQAIYGWRGASADNLRQLNEDYPHLHVVKLEQNYRSTVRILKAANSLIANNPKLFE
CCCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
KRLWSDLGHGDAIQVMPTKDDEHEAESVVMRLLSHKFQHRTEWRDYAILYRGNYQARVFE
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHH
QALRNEKVPYQLSGGQSFFDKAEIKDVIAYLRLIANSDDDPAFIRAVTTPKRGIGAATLE
HHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHH
KLGQVAATLHVSLFEAVFSAAAASQIGERHLTPLTEFCNFINRMEERVAREPAGELLDDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LKTIGYEVYLFDQDDQRAAQSKWNNVLEFAAWITKKGEEDEKNLLQLTQTIALITLLEGR
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
EEEESDAVRLSTLHAAKGLEFGHVFLVGVEENILPHRDAVDEGRLEEERRLMYVGVTRAR
CCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHEEEEEEHHHHH
KSLTLSYCSRRKRARESVACDPSRFIDELGDEVRMPEKPTTAEAKASGSDRLAALKAMLG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHC
>Mature Secondary Structure 
SLLAALNPPQREAAKYLDGPLLVLAGAGSGKTRVITHKIAYLIGECGYKPGSIAAITFT
CCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEC
NKAAREMAERAAKLTHGVNTKGLIVTTFHSMGLRMLREDAKFAELKPAFSILDSADAAGI
CHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHH
VSEIIKTTDKQEIRRAVSRISLWKNELKSPAAALASAEDDNARVYAKIYDRYDATLRAYQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHH
AVDFDDLIRLPAELLDTHAELREKWQNRLRHILVDEYQDTNVAQYRLLQKLTGVRAAFTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
VGDDDQAIYGWRGASADNLRQLNEDYPHLHVVKLEQNYRSTVRILKAANSLIANNPKLFE
CCCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
KRLWSDLGHGDAIQVMPTKDDEHEAESVVMRLLSHKFQHRTEWRDYAILYRGNYQARVFE
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHH
QALRNEKVPYQLSGGQSFFDKAEIKDVIAYLRLIANSDDDPAFIRAVTTPKRGIGAATLE
HHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHH
KLGQVAATLHVSLFEAVFSAAAASQIGERHLTPLTEFCNFINRMEERVAREPAGELLDDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LKTIGYEVYLFDQDDQRAAQSKWNNVLEFAAWITKKGEEDEKNLLQLTQTIALITLLEGR
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
EEEESDAVRLSTLHAAKGLEFGHVFLVGVEENILPHRDAVDEGRLEEERRLMYVGVTRAR
CCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHEEEEEEHHHHH
KSLTLSYCSRRKRARESVACDPSRFIDELGDEVRMPEKPTTAEAKASGSDRLAALKAMLG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]