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The map label for this gene is mnmC

Identifier: 161899033

GI number: 161899033

Start: 1724603

End: 1726453

Strand: Reverse

Name: mnmC

Synonym: Tbd_1641

Alternate gene names: 161899033

Gene position: 1726453-1724603 (Counterclockwise)

Preceding gene: 74317661

Following gene: 74317655

Centisome position: 59.33

GC content: 71.04

Gene sequence:

>1851_bases
ATGCTCAGAACCATCGTCCCCGCCCGACTCGAATTCCGCGACGGCGTCCCGTATTCCGCGGCCTACGGCGACGTTTACCA
TTCTGCCGACGGCGGCCCCGGCCAGGCCCGCCATGTCTTTCTTGCCGGCTGCGGCCTGCCCGCCGCCTGGGCCGGCCGCG
ACAGCTTCGTCGTGCTCGAGACCGGTTTCGGCACCGGGCTGAATTTTCTCGCGACCTGGGCGGCGTGGCGTGACGACCCC
GCCCGGCCGTCGCGGCTGCATTTCCTGTCGGTCGAAAAACACCCGTTCCAGGCGGCCGACCTCGCCCGCATTCACGCGCA
ATGGCCCGAATTCGCTGAGCTGTCCGAGGTGCTGCGCGGCAATTGGCCCATGCTTCTGCCGGGATTCCACCGCGTCGCGC
TCGACGGCGGCCGCGTGCAGCTCACGCTGATGCTCGGCGAGGCGGCGGAATGCCTGCACGAGGTCGAGGCGCGCGTGGAC
GCGTTCTATCTCGACGGCTTCGCCCCCGACCGCAACACGGATCTGTGGCAACCCCAATTATTTGAAACCCTGGCGTGGCT
CGCGAACCCGGGGGCAACGGCCGCGACCTACACCGTCGCGGCGCCGGTGTTACAGGGGCTGACCCAAGCCGGCTTCGCCT
GCGAAAAACGTCGCGGCTACGGCCGCAAGCGTCATTGCATGAGCGCGCGTTTCGCAGGCCGATCACGCGCGGAGGTGTCC
GCTGCCCCCCGGCATGTCGCGGTCATCGGCGGCGGCGTCGCGGGCGCGGCGGCAGCGCGCGCGCTGGTCGACCGCGGCGT
GTCGGTAACGCTGCTCGAGCGCGCAACCGCGCCGGCCGAGGGGGCTTCGGGCAATCCGGTCGCAGTGTTCCGGCCGCTGA
TTTCGCGCGACGACAACCGCGCGACGCGCCTCACACGGGCGGCTTTCCTTCACGATCTGCGCGCGTGGGCCGCGCTCGGC
GAGTGCGTGCAATGGGCGCGTTGCGGCGTCCTGCACCTCGCGCGCGACGCGGCGACCGCCGCGAAGCAGCAGCAGGCCTT
GGCCGAGATTGCCCTCCCGGCCGATTACGCGCGCTGGGTGGACTTGGGCGAGGCGCGCGAACTCGCGCACTGGTCCGTCG
CCGCGCCCGGCACGTTCTACTCGGCGGCGGGCTGGGTCGTGCCCGGAAGCCTGTGCCGCGCCTGGCTCGACCACCCCGGC
ATCGGCTTGCAGACCAATCGGGCGGTCGGGCGTCTCCAAGCGGTCTCGAACGGCTGGCAGATCCTGGATCGCGAAGGCCA
CGCGCTAGCCGAAGCCGACGCGGTCGTGCTCGCCAACGCACGCGACGCGTCGCGTCTCGCCCTCGGTCAAGCCTGGCCGC
TCCACACTGTGCGCGGGCAGGTCACGCAACTGCCGGCGGGCAGCCTGCCGGAAATCGCCCGTGTGATCGCGCGCGAAGGC
TACGTCGCGCCGGGGGCCGCCGGGCCGCTGGTCGGCGCGACTTACGAACATCCTGAAGGGTACAAGGAGAACGACGACGA
CACCGCGCCGCGCGCGGCGAGCGACGTCGCCAATCTCGCGCGCCTCGAGGCCATTCTGCCCGGCGCTACTCAGCGATTCG
TGTCGAATGAGGTGAGCGGCCGCGCCTCGCTGCGCGCGACCCTGCCGGATCGCCTGCCGCTCGTCGGGGCAGTCGATGGA
CAACCGGGGTTGTATGTGGCGGCGGGTTACGCCTCGCGCGGCGTCGTCTGGGCGGGGCTGCTCGGGGAGGCGCTTGCCGA
CCTGATCACCGGCCAGCCGCTGCCTTTGGAAGCCGAGTTGATGCGAGGAATCGCGCCGGCGCGGTTTGCCGGCAACAGAA
AGACGGCCTGA

Upstream 100 bases:

>100_bases
ATTCCCCCAAACTCAGTTCGATGGGTCGTGCGATATGGCCGCGCCACCGGGAGTCACCCGGGGGAAAAGGAGCGGACGCG
CCGATGTAACATTGCGGCCC

Downstream 100 bases:

>100_bases
AGCGAAACTGGGCCGTGGCGTTGCCGCAGCGGATCAGGCGGCGGTGAGCGGTTTTCCCGTGCGCGCGGCAACCGCGAGTC
CCGCCTGGTCGTAAAGCCCA

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MLRTIVPARLEFRDGVPYSAAYGDVYHSADGGPGQARHVFLAGCGLPAAWAGRDSFVVLETGFGTGLNFLATWAAWRDDP
ARPSRLHFLSVEKHPFQAADLARIHAQWPEFAELSEVLRGNWPMLLPGFHRVALDGGRVQLTLMLGEAAECLHEVEARVD
AFYLDGFAPDRNTDLWQPQLFETLAWLANPGATAATYTVAAPVLQGLTQAGFACEKRRGYGRKRHCMSARFAGRSRAEVS
AAPRHVAVIGGGVAGAAAARALVDRGVSVTLLERATAPAEGASGNPVAVFRPLISRDDNRATRLTRAAFLHDLRAWAALG
ECVQWARCGVLHLARDAATAAKQQQALAEIALPADYARWVDLGEARELAHWSVAAPGTFYSAAGWVVPGSLCRAWLDHPG
IGLQTNRAVGRLQAVSNGWQILDREGHALAEADAVVLANARDASRLALGQAWPLHTVRGQVTQLPAGSLPEIARVIAREG
YVAPGAAGPLVGATYEHPEGYKENDDDTAPRAASDVANLARLEAILPGATQRFVSNEVSGRASLRATLPDRLPLVGAVDG
QPGLYVAAGYASRGVVWAGLLGEALADLITGQPLPLEAELMRGIAPARFAGNRKTA

Sequences:

>Translated_616_residues
MLRTIVPARLEFRDGVPYSAAYGDVYHSADGGPGQARHVFLAGCGLPAAWAGRDSFVVLETGFGTGLNFLATWAAWRDDP
ARPSRLHFLSVEKHPFQAADLARIHAQWPEFAELSEVLRGNWPMLLPGFHRVALDGGRVQLTLMLGEAAECLHEVEARVD
AFYLDGFAPDRNTDLWQPQLFETLAWLANPGATAATYTVAAPVLQGLTQAGFACEKRRGYGRKRHCMSARFAGRSRAEVS
AAPRHVAVIGGGVAGAAAARALVDRGVSVTLLERATAPAEGASGNPVAVFRPLISRDDNRATRLTRAAFLHDLRAWAALG
ECVQWARCGVLHLARDAATAAKQQQALAEIALPADYARWVDLGEARELAHWSVAAPGTFYSAAGWVVPGSLCRAWLDHPG
IGLQTNRAVGRLQAVSNGWQILDREGHALAEADAVVLANARDASRLALGQAWPLHTVRGQVTQLPAGSLPEIARVIAREG
YVAPGAAGPLVGATYEHPEGYKENDDDTAPRAASDVANLARLEAILPGATQRFVSNEVSGRASLRATLPDRLPLVGAVDG
QPGLYVAAGYASRGVVWAGLLGEALADLITGQPLPLEAELMRGIAPARFAGNRKTA
>Mature_616_residues
MLRTIVPARLEFRDGVPYSAAYGDVYHSADGGPGQARHVFLAGCGLPAAWAGRDSFVVLETGFGTGLNFLATWAAWRDDP
ARPSRLHFLSVEKHPFQAADLARIHAQWPEFAELSEVLRGNWPMLLPGFHRVALDGGRVQLTLMLGEAAECLHEVEARVD
AFYLDGFAPDRNTDLWQPQLFETLAWLANPGATAATYTVAAPVLQGLTQAGFACEKRRGYGRKRHCMSARFAGRSRAEVS
AAPRHVAVIGGGVAGAAAARALVDRGVSVTLLERATAPAEGASGNPVAVFRPLISRDDNRATRLTRAAFLHDLRAWAALG
ECVQWARCGVLHLARDAATAAKQQQALAEIALPADYARWVDLGEARELAHWSVAAPGTFYSAAGWVVPGSLCRAWLDHPG
IGLQTNRAVGRLQAVSNGWQILDREGHALAEADAVVLANARDASRLALGQAWPLHTVRGQVTQLPAGSLPEIARVIAREG
YVAPGAAGPLVGATYEHPEGYKENDDDTAPRAASDVANLARLEAILPGATQRFVSNEVSGRASLRATLPDRLPLVGAVDG
QPGLYVAAGYASRGVVWAGLLGEALADLITGQPLPLEAELMRGIAPARFAGNRKTA

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=657, Percent_Identity=34.2465753424658, Blast_Score=310, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_THIDA (Q3SID4)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315399.2
- STRING:   Q3SID4
- GeneID:   3673026
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1641
- NMPDR:   fig|292415.3.peg.1738
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- ProtClustDB:   PRK01747
- BioCyc:   TDEN292415:TBD_1641-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 65573; Mature: 65573

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTIVPARLEFRDGVPYSAAYGDVYHSADGGPGQARHVFLAGCGLPAAWAGRDSFVVLE
CCCCCCCCCEECCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEE
TGFGTGLNFLATWAAWRDDPARPSRLHFLSVEKHPFQAADLARIHAQWPEFAELSEVLRG
CCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHC
NWPMLLPGFHRVALDGGRVQLTLMLGEAAECLHEVEARVDAFYLDGFAPDRNTDLWQPQL
CCCEECCCCEEEEECCCEEEEEEEECHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHH
FETLAWLANPGATAATYTVAAPVLQGLTQAGFACEKRRGYGRKRHCMSARFAGRSRAEVS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCHHH
AAPRHVAVIGGGVAGAAAARALVDRGVSVTLLERATAPAEGASGNPVAVFRPLISRDDNR
CCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEHHHCCCCCH
ATRLTRAAFLHDLRAWAALGECVQWARCGVLHLARDAATAAKQQQALAEIALPADYARWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DLGEARELAHWSVAAPGTFYSAAGWVVPGSLCRAWLDHPGIGLQTNRAVGRLQAVSNGWQ
CCHHHHHHHHEEECCCCCHHCCCCCCCCHHHHHHHHCCCCCCEECCHHHHHHHHHCCCCE
ILDREGHALAEADAVVLANARDASRLALGQAWPLHTVRGQVTQLPAGSLPEIARVIAREG
EEECCCCEECCCCEEEEECCCCCHHHHHCCCCCCHHHCCCCEECCCCCHHHHHHHHHHCC
YVAPGAAGPLVGATYEHPEGYKENDDDTAPRAASDVANLARLEAILPGATQRFVSNEVSG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
RASLRATLPDRLPLVGAVDGQPGLYVAAGYASRGVVWAGLLGEALADLITGQPLPLEAEL
CCEEEECCCCCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
MRGIAPARFAGNRKTA
HCCCCCHHHCCCCCCC
>Mature Secondary Structure
MLRTIVPARLEFRDGVPYSAAYGDVYHSADGGPGQARHVFLAGCGLPAAWAGRDSFVVLE
CCCCCCCCCEECCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEE
TGFGTGLNFLATWAAWRDDPARPSRLHFLSVEKHPFQAADLARIHAQWPEFAELSEVLRG
CCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHC
NWPMLLPGFHRVALDGGRVQLTLMLGEAAECLHEVEARVDAFYLDGFAPDRNTDLWQPQL
CCCEECCCCEEEEECCCEEEEEEEECHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHH
FETLAWLANPGATAATYTVAAPVLQGLTQAGFACEKRRGYGRKRHCMSARFAGRSRAEVS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCHHH
AAPRHVAVIGGGVAGAAAARALVDRGVSVTLLERATAPAEGASGNPVAVFRPLISRDDNR
CCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEHHHCCCCCH
ATRLTRAAFLHDLRAWAALGECVQWARCGVLHLARDAATAAKQQQALAEIALPADYARWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DLGEARELAHWSVAAPGTFYSAAGWVVPGSLCRAWLDHPGIGLQTNRAVGRLQAVSNGWQ
CCHHHHHHHHEEECCCCCHHCCCCCCCCHHHHHHHHCCCCCCEECCHHHHHHHHHCCCCE
ILDREGHALAEADAVVLANARDASRLALGQAWPLHTVRGQVTQLPAGSLPEIARVIAREG
EEECCCCEECCCCEEEEECCCCCHHHHHCCCCCCHHHCCCCEECCCCCHHHHHHHHHHCC
YVAPGAAGPLVGATYEHPEGYKENDDDTAPRAASDVANLARLEAILPGATQRFVSNEVSG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
RASLRATLPDRLPLVGAVDGQPGLYVAAGYASRGVVWAGLLGEALADLITGQPLPLEAEL
CCEEEECCCCCCCEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
MRGIAPARFAGNRKTA
HCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA