BacMap

Definition	Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence.
Accession	NC_007348
Length	2,726,152

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The map label for this gene is gra-orf6 [H]

Identifier: 73539674

GI number: 73539674

Start: 2692060

End: 2692809

Strand: Reverse

Name: gra-orf6 [H]

Synonym: Reut_B5854

Alternate gene names: 73539674

Gene position: 2692809-2692060 (Counterclockwise)

Preceding gene: 73539675

Following gene: 73539673

Centisome position: 98.78

GC content: 68.27

Gene sequence:

>750_bases
ATGAGCCTCTCTTCTCTCTCCGGCAAGGTTGCCGTGATCCTCGGCGGTACTGGCGGCATCGGCCTGGCGACAGGCACTGC
GCTGGCGCAGCTTGGCGCGAGCGTCGTCCTGACCGGGCGCAATCGCGATACCGCGGCCAGCGCGGCGGCTTCGCTGCCGG
CCGGCAATCACATGGGCGCAGTGGCCGAGATCGGCGACACCGCATCGCTAAACGCGCTCGCCGCCGAAGTCGCTCACCAC
TACGGCCGCGTCGACATCCTCGTCAATACCGCAGGCTTCACGCAGGCCATTCCGCACGCCGATCTCGACGCGCTCAACGA
TGCACTGATCGACGAGCTGTTCGCGATCAACTGGCGCGGCCAGTTCGCGGCGATCCGCGCATTTGCGCCGCATCTTCGCG
CCAATGGCGATGGACTGGTGGTCAACGTAGGCTCGATCGCGGGCCGAACGGGCCAGGGCAGCAACGTGGCGTACTGCGCC
GCCAAAGCCGGTCTCGATGTCATGGCCGTGTCGCTGGGCCGCGCGCTTGCGCCGGCCATCCGCGTACTGAACGTGTCGCC
GGGCGTGGTCGATACCGGCTTTGTGCCCGGCCGCGACGACACCTTCAACGAACGCGCGGCCAAAACCACGCCGCTGCGCC
GCATCGGCCAGGCACAGGACGTGGCCGACGCCATCGTCGCCTGCGCGACTTCGCTGCGCTTTGCTACCGGCACCACCATC
GTCGTCGATGGCGGCCGTCAGCTCGGCTGA

Upstream 100 bases:

>100_bases
CTTCGACTTTACGCTCGAACCGGGCGAGCAGACCTTCCCTAAGCCGCCGATCGACTGACGACCCGGTCCTCCACATTTCC
ACGCAGCACAACTGAACACC

Downstream 100 bases:

>100_bases
TCCATTTCCACCCTTAGCCCAACGCAACCATGCACGCTCGCAAGACCATCATCACCTGCGCGGTGACAGGCAATATCGTC
ACGCCTGAGCAGTACCCCGA

Product: NAD-dependent epimerase/dehydratase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: ORF6 [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSLSSLSGKVAVILGGTGGIGLATGTALAQLGASVVLTGRNRDTAASAAASLPAGNHMGAVAEIGDTASLNALAAEVAHH
YGRVDILVNTAGFTQAIPHADLDALNDALIDELFAINWRGQFAAIRAFAPHLRANGDGLVVNVGSIAGRTGQGSNVAYCA
AKAGLDVMAVSLGRALAPAIRVLNVSPGVVDTGFVPGRDDTFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTI
VVDGGRQLG

Sequences:

>Translated_249_residues
MSLSSLSGKVAVILGGTGGIGLATGTALAQLGASVVLTGRNRDTAASAAASLPAGNHMGAVAEIGDTASLNALAAEVAHH
YGRVDILVNTAGFTQAIPHADLDALNDALIDELFAINWRGQFAAIRAFAPHLRANGDGLVVNVGSIAGRTGQGSNVAYCA
AKAGLDVMAVSLGRALAPAIRVLNVSPGVVDTGFVPGRDDTFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTI
VVDGGRQLG
>Mature_248_residues
SLSSLSGKVAVILGGTGGIGLATGTALAQLGASVVLTGRNRDTAASAAASLPAGNHMGAVAEIGDTASLNALAAEVAHHY
GRVDILVNTAGFTQAIPHADLDALNDALIDELFAINWRGQFAAIRAFAPHLRANGDGLVVNVGSIAGRTGQGSNVAYCAA
KAGLDVMAVSLGRALAPAIRVLNVSPGVVDTGFVPGRDDTFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTIV
VDGGRQLG

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=32.8, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI32483357, Length=246, Percent_Identity=30.0813008130081, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI4758504, Length=253, Percent_Identity=30.0395256916996, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI5031737, Length=245, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI15277342, Length=253, Percent_Identity=28.0632411067194, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI59889578, Length=231, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI142976729, Length=203, Percent_Identity=29.064039408867, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI126723750, Length=247, Percent_Identity=27.5303643724696, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI7706318, Length=201, Percent_Identity=29.8507462686567, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI19923817, Length=261, Percent_Identity=28.3524904214559, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI10190704, Length=252, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI33667109, Length=193, Percent_Identity=30.5699481865285, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI83715985, Length=252, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI20149619, Length=201, Percent_Identity=28.8557213930348, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787335, Length=246, Percent_Identity=30.4878048780488, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI2367175, Length=253, Percent_Identity=32.4110671936759, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=30.3846153846154, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787905, Length=257, Percent_Identity=28.0155642023346, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790717, Length=255, Percent_Identity=27.4509803921569, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786812, Length=256, Percent_Identity=31.25, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788459, Length=251, Percent_Identity=30.2788844621514, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082160, Length=256, Percent_Identity=26.953125, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI2367365, Length=248, Percent_Identity=31.0483870967742, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789208, Length=254, Percent_Identity=29.5275590551181, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789057, Length=261, Percent_Identity=28.3524904214559, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=32.2709163346614, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560676, Length=247, Percent_Identity=30.7692307692308, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=29.7709923664122, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=33.6, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562906, Length=266, Percent_Identity=31.9548872180451, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=28.8389513108614, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560332, Length=268, Percent_Identity=32.4626865671642, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551412, Length=265, Percent_Identity=32.4528301886792, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI193204405, Length=267, Percent_Identity=30.7116104868914, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17560220, Length=269, Percent_Identity=31.2267657992565, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=266, Percent_Identity=31.9548872180451, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=243, Percent_Identity=28.8065843621399, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=30.6451612903226, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538480, Length=269, Percent_Identity=32.3420074349442, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560150, Length=267, Percent_Identity=29.5880149812734, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17565030, Length=265, Percent_Identity=29.811320754717, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17562904, Length=269, Percent_Identity=31.9702602230483, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17562990, Length=269, Percent_Identity=28.6245353159851, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=265, Percent_Identity=28.6792452830189, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=270, Percent_Identity=31.1111111111111, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=269, Percent_Identity=29.7397769516729, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17561402, Length=256, Percent_Identity=28.90625, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538486, Length=271, Percent_Identity=29.1512915129151, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=28.6792452830189, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17564282, Length=200, Percent_Identity=28.5, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17538182, Length=263, Percent_Identity=28.5171102661597, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17567345, Length=161, Percent_Identity=26.7080745341615, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=28.6290322580645, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193203117, Length=238, Percent_Identity=27.7310924369748, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=228, Percent_Identity=28.0701754385965, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=243, Percent_Identity=31.2757201646091, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324126, Length=252, Percent_Identity=27.3809523809524, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI23397609, Length=251, Percent_Identity=33.0677290836653, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24639444, Length=261, Percent_Identity=29.5019157088123, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21355319, Length=251, Percent_Identity=29.0836653386454, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=30.078125, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357041, Length=258, Percent_Identity=30.6201550387597, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24644339, Length=254, Percent_Identity=30.7086614173228, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644337, Length=254, Percent_Identity=31.496062992126, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24643142, Length=251, Percent_Identity=31.4741035856574, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364892, Length=199, Percent_Identity=27.6381909547739, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737361, Length=267, Percent_Identity=26.5917602996255, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581726, Length=203, Percent_Identity=30.0492610837438, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24640492, Length=199, Percent_Identity=26.1306532663317, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 25005; Mature: 24873

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSSLSGKVAVILGGTGGIGLATGTALAQLGASVVLTGRNRDTAASAAASLPAGNHMGA
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCC
VAEIGDTASLNALAAEVAHHYGRVDILVNTAGFTQAIPHADLDALNDALIDELFAINWRG
HHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHEECCCC
QFAAIRAFAPHLRANGDGLVVNVGSIAGRTGQGSNVAYCAAKAGLDVMAVSLGRALAPAI
CCEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHH
RVLNVSPGVVDTGFVPGRDDTFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTI
EEEECCCCCEECCCCCCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCEE
VVDGGRQLG
EEECCCCCC
>Mature Secondary Structure 
SLSSLSGKVAVILGGTGGIGLATGTALAQLGASVVLTGRNRDTAASAAASLPAGNHMGA
CCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCCC
VAEIGDTASLNALAAEVAHHYGRVDILVNTAGFTQAIPHADLDALNDALIDELFAINWRG
HHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCHHHHHHHHHHHHHEECCCC
QFAAIRAFAPHLRANGDGLVVNVGSIAGRTGQGSNVAYCAAKAGLDVMAVSLGRALAPAI
CCEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHH
RVLNVSPGVVDTGFVPGRDDTFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTI
EEEECCCCCEECCCCCCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCEE
VVDGGRQLG
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2583128; 9831526 [H]