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The map label for this gene is mhpE [C]

Identifier: 73539643

GI number: 73539643

Start: 2651465

End: 2652487

Strand: Reverse

Name: mhpE [C]

Synonym: Reut_B5823

Alternate gene names: 73539643

Gene position: 2652487-2651465 (Counterclockwise)

Preceding gene: 73539644

Following gene: 73539642

Centisome position: 97.3

GC content: 65.69

Gene sequence:

>1023_bases
ATGCCTTTCAACCCCGGCAAGAAGCTCTACATTTCCGACGTGACGCTGCGTGACGGCAGCCACGCCATCCGCCACCAGTA
TTCGATTCAGAACGTCAAGGACATCGCCCGCGCGCTCGACAAGGCCCGCGTCGATTCCATCGAAGTCGCCCACGGAGACG
GCCTGCAGGGCTCCAGCTTCAACTACGGCTTCGGCGCCCACACCGACCTGGAGTGGATCGAGGCCGTGGCCGGCGAGATC
TCGCACGCGCAGATCGCCACGCTGCTGCTGCCCGGCATCGGCACCATCCATGACCTGGACAACGCCTACAAGGCCGGCGC
GCGCATCGTGCGCGTGGCCACGCACTGCACCGAGGCCGACGTCTCGCGCCAGCACATCGAGCGTGCGCGCGAACTGGGCA
TGGATACGGTCGGCTTCCTGATGATGAGCCACATGACGACGCCGGAAAACCTGGCGGTCGAGGCGAAGAAGATGGAAAGC
TACGGCGCGACCACGATCTACGTGGTCGATTCCGGCGGCGCGCTCAGCATGGATGATGTCCGGGCCCGCTTCCGCGCGCT
CAAGGCCGTGCTCGACCCGTCGACCACGACGGGTATGCACGCCCACCACAATTTGTCGCTCGGCGTGGCCAATTCGATCG
TCGCCGTCGAAGAAGGCTGCGACCGCATCGACGCCAGCCTCGCCGGCATGGGCGCCGGCGCCGGCAATGCGCCGCTGGAA
GTCTTCATCGCCGCCGCCGACCGCATGGGCTGGGACCACGGCACCGATCTGTACACGCTGATGGACGCCGCCGATGACAT
CGTCCGTCCGCTGCAGGACCGCCCGGTGCGCGTCGACCGCGAGACCTTGGCACTAGGCTACGCAGGCGTGTATTCGAGCT
TCCTGCGCCACTCCGAAGTCGCCGCCAACAAGTACGGCATCAAGACCGTCGACATCCTGGTCGAGCTTGGCAAGCGCCGC
ATGGTCGGCGGCCAGGAAGACATGATTGTCGACGTGGCGCTGGACCTGCTCAACAAGGCATAA

Upstream 100 bases:

>100_bases
CCCGCCTACGCCGGCAATCTGGACATCATGACCTCCGCCGCGCTGGCCACCGCCGAGCGCATGGCGCAGGCCGCACTGAC
TGCTTGAGGAGACACCCGCC

Downstream 100 bases:

>100_bases
AGCGATTGGGCCTGCGTTCGGCCTACGCGCGCGCGAAGTGCCGCCGGACACCTCCGGCGGCGTTACACTTGCCGGCATGA
CCGACACCACCCATCCCACC

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MPFNPGKKLYISDVTLRDGSHAIRHQYSIQNVKDIARALDKARVDSIEVAHGDGLQGSSFNYGFGAHTDLEWIEAVAGEI
SHAQIATLLLPGIGTIHDLDNAYKAGARIVRVATHCTEADVSRQHIERARELGMDTVGFLMMSHMTTPENLAVEAKKMES
YGATTIYVVDSGGALSMDDVRARFRALKAVLDPSTTTGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLE
VFIAAADRMGWDHGTDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEVAANKYGIKTVDILVELGKRR
MVGGQEDMIVDVALDLLNKA

Sequences:

>Translated_340_residues
MPFNPGKKLYISDVTLRDGSHAIRHQYSIQNVKDIARALDKARVDSIEVAHGDGLQGSSFNYGFGAHTDLEWIEAVAGEI
SHAQIATLLLPGIGTIHDLDNAYKAGARIVRVATHCTEADVSRQHIERARELGMDTVGFLMMSHMTTPENLAVEAKKMES
YGATTIYVVDSGGALSMDDVRARFRALKAVLDPSTTTGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLE
VFIAAADRMGWDHGTDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEVAANKYGIKTVDILVELGKRR
MVGGQEDMIVDVALDLLNKA
>Mature_339_residues
PFNPGKKLYISDVTLRDGSHAIRHQYSIQNVKDIARALDKARVDSIEVAHGDGLQGSSFNYGFGAHTDLEWIEAVAGEIS
HAQIATLLLPGIGTIHDLDNAYKAGARIVRVATHCTEADVSRQHIERARELGMDTVGFLMMSHMTTPENLAVEAKKMESY
GATTIYVVDSGGALSMDDVRARFRALKAVLDPSTTTGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEV
FIAAADRMGWDHGTDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEVAANKYGIKTVDILVELGKRRM
VGGQEDMIVDVALDLLNKA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=334, Percent_Identity=85.0299401197605, Blast_Score=565, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_CUPPJ (Q46NW8)

Other databases:

- EMBL:   CP000091
- RefSeq:   YP_300010.1
- ProteinModelPortal:   Q46NW8
- SMR:   Q46NW8
- GeneID:   3614065
- GenomeReviews:   CP000091_GR
- KEGG:   reu:Reut_B5823
- NMPDR:   fig|264198.3.peg.6471
- HOGENOM:   HBG300122
- OMA:   RRAYDIG
- ProtClustDB:   PRK08195
- BioCyc:   REUT264198:REUT_B5823-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36712; Mature: 36581

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 171-171 BINDING 200-200 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFNPGKKLYISDVTLRDGSHAIRHQYSIQNVKDIARALDKARVDSIEVAHGDGLQGSSF
CCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
NYGFGAHTDLEWIEAVAGEISHAQIATLLLPGIGTIHDLDNAYKAGARIVRVATHCTEAD
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHEEHHHHHCCHHH
VSRQHIERARELGMDTVGFLMMSHMTTPENLAVEAKKMESYGATTIYVVDSGGALSMDDV
HHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
RARFRALKAVLDPSTTTGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLE
HHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCE
VFIAAADRMGWDHGTDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEV
EEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHH
AANKYGIKTVDILVELGKRRMVGGQEDMIVDVALDLLNKA
HHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFNPGKKLYISDVTLRDGSHAIRHQYSIQNVKDIARALDKARVDSIEVAHGDGLQGSSF
CCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
NYGFGAHTDLEWIEAVAGEISHAQIATLLLPGIGTIHDLDNAYKAGARIVRVATHCTEAD
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHEEHHHHHCCHHH
VSRQHIERARELGMDTVGFLMMSHMTTPENLAVEAKKMESYGATTIYVVDSGGALSMDDV
HHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
RARFRALKAVLDPSTTTGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLE
HHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCE
VFIAAADRMGWDHGTDLYTLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEV
EEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHH
AANKYGIKTVDILVELGKRRMVGGQEDMIVDVALDLLNKA
HHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA