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The map label for this gene is yagI [H]

Identifier: 73539642

GI number: 73539642

Start: 2650618

End: 2651502

Strand: Reverse

Name: yagI [H]

Synonym: Reut_B5822

Alternate gene names: 73539642

Gene position: 2651502-2650618 (Counterclockwise)

Preceding gene: 73539643

Following gene: 73539638

Centisome position: 97.26

GC content: 67.23

Gene sequence:

>885_bases
TTGTCGACGTGGCGCTGGACCTGCTCAACAAGGCATAAAGCGATTGGGCCTGCGTTCGGCCTACGCGCGCGCGAAGTGCC
GCCGGACACCTCCGGCGGCGTTACACTTGCCGGCATGACCGACACCACCCATCCCACCCGCGCCCTCGCCAGCCAGACGC
TTTTCCGCGGCCTGGACGTGGTCGATGCCGTCGCGGCCGGTTCCCAGACCGTGCAGGACATCGCGAACCGCACCGGACTG
CCGTTCAGCACCACGCACCGGCTGGCGTCGGCCCTAGTGCAGGCGCGCTACCTCCATTTCGAGCCGCGACGCGGCTACCG
GCTGGGCCCGCGCCTGCTGGAACTCGGCTTCGCCGCCTACCGCGACTCGGACATTACGCGCAGCGCGCGCGCCGGCATGG
AAGAACTGGCCGAGCAAACCCGGGACACCATTCACCTCGCCCAACTCGACGGCGACGAGATCATCTACCTCGACAAGATC
GGCGGGCAGCGCCCGATCAGCGTCAATTCGCGCATTGGCGGCCGCAAGCCGGTCTGCTCGACCGGCGTGGGCAAGGCGCT
GATCCTCGACGAGACCGAGGACAAGTGGAAAGCTCGCTACGAATACGATGCGAAGCGGGGCAACGTGCAGCTTCCTCTCG
ATCGGTGGCTCGCCGGCATGCGCGTGTACGCGCAGGGCGGCTATGCCTTTGACCTTGAAGAAGACGCGCCGAGCATCTGC
TGCGTGGCGGTGCCCATCCGCGATGCCAGCGGCCAGACGGTGGCAGCAATCAGCGTCACGGGGACGACCGATTACACCGA
CGAAGCGCAACTGCGAGCGCTGATTCCGGTGGTGCGGCGGGTCGCGCAATCCATCAGCGAGAAGATCGGCGGCAGGCTGG
GGTGA

Upstream 100 bases:

>100_bases
CGCCACTCCGAAGTCGCCGCCAACAAGTACGGCATCAAGACCGTCGACATCCTGGTCGAGCTTGGCAAGCGCCGCATGGT
CGGCGGCCAGGAAGACATGA

Downstream 100 bases:

>100_bases
GGGCCTGTCCGGGGCAGCAGGGGTGAACCTGCCCGAGTTTTTGTCGTACTCCCGAATCGGACATCACCCGACCAACGACA
GCCGCGCAATTTCCGAGGCG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSTWRWTCSTRHKAIGPAFGLRAREVPPDTSGGVTLAGMTDTTHPTRALASQTLFRGLDVVDAVAAGSQTVQDIANRTGL
PFSTTHRLASALVQARYLHFEPRRGYRLGPRLLELGFAAYRDSDITRSARAGMEELAEQTRDTIHLAQLDGDEIIYLDKI
GGQRPISVNSRIGGRKPVCSTGVGKALILDETEDKWKARYEYDAKRGNVQLPLDRWLAGMRVYAQGGYAFDLEEDAPSIC
CVAVPIRDASGQTVAAISVTGTTDYTDEAQLRALIPVVRRVAQSISEKIGGRLG

Sequences:

>Translated_294_residues
MSTWRWTCSTRHKAIGPAFGLRAREVPPDTSGGVTLAGMTDTTHPTRALASQTLFRGLDVVDAVAAGSQTVQDIANRTGL
PFSTTHRLASALVQARYLHFEPRRGYRLGPRLLELGFAAYRDSDITRSARAGMEELAEQTRDTIHLAQLDGDEIIYLDKI
GGQRPISVNSRIGGRKPVCSTGVGKALILDETEDKWKARYEYDAKRGNVQLPLDRWLAGMRVYAQGGYAFDLEEDAPSIC
CVAVPIRDASGQTVAAISVTGTTDYTDEAQLRALIPVVRRVAQSISEKIGGRLG
>Mature_293_residues
STWRWTCSTRHKAIGPAFGLRAREVPPDTSGGVTLAGMTDTTHPTRALASQTLFRGLDVVDAVAAGSQTVQDIANRTGLP
FSTTHRLASALVQARYLHFEPRRGYRLGPRLLELGFAAYRDSDITRSARAGMEELAEQTRDTIHLAQLDGDEIIYLDKIG
GQRPISVNSRIGGRKPVCSTGVGKALILDETEDKWKARYEYDAKRGNVQLPLDRWLAGMRVYAQGGYAFDLEEDAPSICC
VAVPIRDASGQTVAAISVTGTTDYTDEAQLRALIPVVRRVAQSISEKIGGRLG

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=245, Percent_Identity=31.8367346938775, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788131, Length=224, Percent_Identity=30.8035714285714, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1786715, Length=255, Percent_Identity=25.8823529411765, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082359, Length=251, Percent_Identity=26.2948207171315, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789999, Length=256, Percent_Identity=25.78125, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788581, Length=222, Percent_Identity=28.3783783783784, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790752, Length=254, Percent_Identity=25.9842519685039, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32031; Mature: 31899

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTWRWTCSTRHKAIGPAFGLRAREVPPDTSGGVTLAGMTDTTHPTRALASQTLFRGLDV
CCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VDAVAAGSQTVQDIANRTGLPFSTTHRLASALVQARYLHFEPRRGYRLGPRLLELGFAAY
HHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHH
RDSDITRSARAGMEELAEQTRDTIHLAQLDGDEIIYLDKIGGQRPISVNSRIGGRKPVCS
CCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCCCEEECCCCCCCCCCCC
TGVGKALILDETEDKWKARYEYDAKRGNVQLPLDRWLAGMRVYAQGGYAFDLEEDAPSIC
CCCCCEEEEECCCHHHHHEEEECCCCCCEECCHHHHHHCEEEEECCCEEEEECCCCCCEE
CVAVPIRDASGQTVAAISVTGTTDYTDEAQLRALIPVVRRVAQSISEKIGGRLG
EEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STWRWTCSTRHKAIGPAFGLRAREVPPDTSGGVTLAGMTDTTHPTRALASQTLFRGLDV
CCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VDAVAAGSQTVQDIANRTGLPFSTTHRLASALVQARYLHFEPRRGYRLGPRLLELGFAAY
HHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHH
RDSDITRSARAGMEELAEQTRDTIHLAQLDGDEIIYLDKIGGQRPISVNSRIGGRKPVCS
CCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCCCEEECCCCCCCCCCCC
TGVGKALILDETEDKWKARYEYDAKRGNVQLPLDRWLAGMRVYAQGGYAFDLEEDAPSIC
CCCCCEEEEECCCHHHHHEEEECCCCCCEECCHHHHHHCEEEEECCCEEEEECCCCCCEE
CVAVPIRDASGQTVAAISVTGTTDYTDEAQLRALIPVVRRVAQSISEKIGGRLG
EEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]