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The map label for this gene is citE [H]

Identifier: 73539641

GI number: 73539641

Start: 2649430

End: 2650323

Strand: Direct

Name: citE [H]

Synonym: Reut_B5821

Alternate gene names: 73539641

Gene position: 2649430-2650323 (Clockwise)

Preceding gene: 73539640

Following gene: 73539645

Centisome position: 97.19

GC content: 63.31

Gene sequence:

>894_bases
ATGCACGCCAACATGTCACAACGTCTACGTTCCCTGCTATTCGTTCCCGCGGACAGCGACAGGAAACTCGCCGCAGCACT
CAAGACGGGAGCCGATGGCCTGGTCTTTGACCTCGAAGATGCCGTTCCCGAGCATGGCAAGGAAGAGGCGCGGCGCAAGC
TGGCGGCATTTCTTGGGGAGAACCGTCAAGGCATGGACGCTGCGGTGATGGTGCGGGTCAACGGCCTGGCGTCGGGACAT
ATTCTTCAGGACCTGGCCGCTGTCGTGCCTCACCGTCCCGATGCGATCGTGCTTCCCAAGTGCAGGGGCGTGGCCGATGT
TCATCGCCTGTGCGATTTCCTCGCGGCGCTCGAGTGCGTGTTGCTCGACAGCGCGTGGGAGACCGGCATCGTTGCCATGG
CCACCGAGTCATCGGAAGGGGTGTTTGGCCTGTCTTCATACCGGAATGCCCCGATGCGCCTGCGCGCCTTGATGTGGGGC
GTGGACGATCTCGCGGCCGACCTTGGGGCGCGCTCGTCGCACCAGGGCAGGGAACTCACCGCACCGTTTTCCGTGGCACG
TACGATGTGCCTGATGGCAGCCGCACAGGCTGGGATCGACGCCATCGACGCGGTCAGCACCAATCTGTCCGACGACGCCG
AGATCGGCCACGAGGCGGCGCAGGCAAAGCGCGACGGCTTTACTGCCAAGGCTGCCGCCCATCCCTCCCATATCGAAGCG
ATCAATCAAGCCTTCATGCCCCAGGTGGAAGAGATTCGCTGGGCACGAGAGGTGGTCGAGGCGTTTTCAAAGGTGGGCGC
TTCGGGCGTGGTGAAGCTGGGCAAGACCATGATTGACCGTCCCCACTTGCGACTGGCGCATCGGATCCTTTGCCAGGTGG
AAGGCGAGGACTGA

Upstream 100 bases:

>100_bases
TGTTCGCGGAAATCACTGGCATCGTTAGGCATCAGCACGATGCCCATACCCGTGCCCAGTCTCAGCGATACCTGCCGCAA
TTTCCCAGGAGCAAGCGACC

Downstream 100 bases:

>100_bases
TCGCGCTATGCAGATATCGGTGCGGCGTCTTGCTGCCGCCCGACGGTCCCGAAGGTCGGCACTAAGCGCCGAGAGGCGCC
GGAGCGCCTGTGGAAGAGAA

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MHANMSQRLRSLLFVPADSDRKLAAALKTGADGLVFDLEDAVPEHGKEEARRKLAAFLGENRQGMDAAVMVRVNGLASGH
ILQDLAAVVPHRPDAIVLPKCRGVADVHRLCDFLAALECVLLDSAWETGIVAMATESSEGVFGLSSYRNAPMRLRALMWG
VDDLAADLGARSSHQGRELTAPFSVARTMCLMAAAQAGIDAIDAVSTNLSDDAEIGHEAAQAKRDGFTAKAAAHPSHIEA
INQAFMPQVEEIRWAREVVEAFSKVGASGVVKLGKTMIDRPHLRLAHRILCQVEGED

Sequences:

>Translated_297_residues
MHANMSQRLRSLLFVPADSDRKLAAALKTGADGLVFDLEDAVPEHGKEEARRKLAAFLGENRQGMDAAVMVRVNGLASGH
ILQDLAAVVPHRPDAIVLPKCRGVADVHRLCDFLAALECVLLDSAWETGIVAMATESSEGVFGLSSYRNAPMRLRALMWG
VDDLAADLGARSSHQGRELTAPFSVARTMCLMAAAQAGIDAIDAVSTNLSDDAEIGHEAAQAKRDGFTAKAAAHPSHIEA
INQAFMPQVEEIRWAREVVEAFSKVGASGVVKLGKTMIDRPHLRLAHRILCQVEGED
>Mature_297_residues
MHANMSQRLRSLLFVPADSDRKLAAALKTGADGLVFDLEDAVPEHGKEEARRKLAAFLGENRQGMDAAVMVRVNGLASGH
ILQDLAAVVPHRPDAIVLPKCRGVADVHRLCDFLAALECVLLDSAWETGIVAMATESSEGVFGLSSYRNAPMRLRALMWG
VDDLAADLGARSSHQGRELTAPFSVARTMCLMAAAQAGIDAIDAVSTNLSDDAEIGHEAAQAKRDGFTAKAAAHPSHIEA
INQAFMPQVEEIRWAREVVEAFSKVGASGVVKLGKTMIDRPHLRLAHRILCQVEGED

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=295, Percent_Identity=27.1186440677966, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI87081764, Length=300, Percent_Identity=32.6666666666667, Blast_Score=101, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17557458, Length=300, Percent_Identity=28.6666666666667, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 31918; Mature: 31918

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHANMSQRLRSLLFVPADSDRKLAAALKTGADGLVFDLEDAVPEHGKEEARRKLAAFLGE
CCCCHHHHHHHHEECCCCCCCHHHHHHHCCCCCEEEECHHHCCCCCHHHHHHHHHHHHCC
NRQGMDAAVMVRVNGLASGHILQDLAAVVPHRPDAIVLPKCRGVADVHRLCDFLAALECV
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
LLDSAWETGIVAMATESSEGVFGLSSYRNAPMRLRALMWGVDDLAADLGARSSHQGRELT
HHHCCCCCCEEEEEECCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
APFSVARTMCLMAAAQAGIDAIDAVSTNLSDDAEIGHEAAQAKRDGFTAKAAAHPSHIEA
CHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHHH
INQAFMPQVEEIRWAREVVEAFSKVGASGVVKLGKTMIDRPHLRLAHRILCQVEGED
HHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHANMSQRLRSLLFVPADSDRKLAAALKTGADGLVFDLEDAVPEHGKEEARRKLAAFLGE
CCCCHHHHHHHHEECCCCCCCHHHHHHHCCCCCEEEECHHHCCCCCHHHHHHHHHHHHCC
NRQGMDAAVMVRVNGLASGHILQDLAAVVPHRPDAIVLPKCRGVADVHRLCDFLAALECV
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
LLDSAWETGIVAMATESSEGVFGLSSYRNAPMRLRALMWGVDDLAADLGARSSHQGRELT
HHHCCCCCCEEEEEECCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
APFSVARTMCLMAAAQAGIDAIDAVSTNLSDDAEIGHEAAQAKRDGFTAKAAAHPSHIEA
CHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHHH
INQAFMPQVEEIRWAREVVEAFSKVGASGVVKLGKTMIDRPHLRLAHRILCQVEGED
HHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9457870 [H]