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The map label for this gene is pobB [H]

Identifier: 73539597

GI number: 73539597

Start: 2601208

End: 2602170

Strand: Reverse

Name: pobB [H]

Synonym: Reut_B5777

Alternate gene names: 73539597

Gene position: 2602170-2601208 (Counterclockwise)

Preceding gene: 73539627

Following gene: 73539596

Centisome position: 95.45

GC content: 65.84

Gene sequence:

>963_bases
ATGAACGACCTGCCCCAGTTCACGGGCGCCCTCCTTGAGTTGCGCCTGGAGTCGATTCAATTCCTCGCCCGCGACACGCT
GCTCTACGCCTTCGCGTCTCCGGTGGGCGCCGCGCTGCCGCCGTGCGAGCCGGGTTCCCATATCGGCTTGCACCTGCCCA
ATGGCCTGGTGCGCCAATATTCGCTGCTCGAAGGCGGCCGCGAGCCGCGGCGCTACCAGGTAGGGGTGAAGCGCGACCCC
AAAAGCCGCGGCGGTTCCAGCTTCATGCACGAGCAGTTGCGCCCCGGCGCGGTGATCCAGGTGGAGCCGCCGCGCAACAA
TTTCCCGCTGAATGAAGAGGCCGAGCATTCGGTGCTGGTCGGTGGCGGCATCGGCATCACGCCGATCCTGTGCATGGCGA
GGCGCCTGAAGGCGCTGGGCCGCTCGATGACACTGGTGTACTCGTGCCGCGACCGCGTCGACGTCGCCTTCCTCGAGGAA
CTGCGCAGCTACGAGAACCTGCACCTGCACATCGACGCAGAAGCGGGCGGCGTGCTCGACATCGCCGAAGTAGTGAAGGC
GCATCCGGCCAACACGCACTTCTATTGCTGCGGCCCGGGCCCGATGCTTTCGGCCTTCGAAGCAGCCACGGCCCACCTGC
CTGCGGCGCAGGTCCACGTCGAGTATTTCACCGCGAAGCAGGAAGCCGCGCTGGACGGCGGCTACACGGTCGAACTGAAG
CGCAGCGGCAAATCTCTGCAGGTACCGGGTGGCAAAACCATCCTGCAGGTGCTCAAGGATGCCGGCGTGGAAGTGGCGCA
CTCCTGCGAGGAAGGCGTGTGCGGCGCCTGCGAGACGCGCGTGCTATCCGGCATTCCCGATCACCGCGACGCCATCCTTT
CCGAACCGGAGAAGGCGGCCAACAACACCATGATGATCTGCTGCTCGGGTTGCAAGGGCGAGCGGCTGGTCCTCGATCTG
TAA

Upstream 100 bases:

>100_bases
CGGGCCTCTCCTTATCGGTCCCACGCCGCACGCTTCGGACACCAGGCGCCGGCAGGCATATGCCTCGCCGCATCACTGAA
CGTCGTCACTGGAGACACAC

Downstream 100 bases:

>100_bases
GTCATTTCATCCCAACCGGAGACATCCCATGATCATTGACTGCCACGGCCACGTCAGCCCGCCCGCCGAACTCTGGGTCT
ACAAGGCTCACATCCTGTCG

Product: phthalate 4,5-dioxygenase

Products: NA

Alternate protein names: 4-carboxydiphenyl ether; phenoxybenzoate dioxygenase subunit beta [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MNDLPQFTGALLELRLESIQFLARDTLLYAFASPVGAALPPCEPGSHIGLHLPNGLVRQYSLLEGGREPRRYQVGVKRDP
KSRGGSSFMHEQLRPGAVIQVEPPRNNFPLNEEAEHSVLVGGGIGITPILCMARRLKALGRSMTLVYSCRDRVDVAFLEE
LRSYENLHLHIDAEAGGVLDIAEVVKAHPANTHFYCCGPGPMLSAFEAATAHLPAAQVHVEYFTAKQEAALDGGYTVELK
RSGKSLQVPGGKTILQVLKDAGVEVAHSCEEGVCGACETRVLSGIPDHRDAILSEPEKAANNTMMICCSGCKGERLVLDL

Sequences:

>Translated_320_residues
MNDLPQFTGALLELRLESIQFLARDTLLYAFASPVGAALPPCEPGSHIGLHLPNGLVRQYSLLEGGREPRRYQVGVKRDP
KSRGGSSFMHEQLRPGAVIQVEPPRNNFPLNEEAEHSVLVGGGIGITPILCMARRLKALGRSMTLVYSCRDRVDVAFLEE
LRSYENLHLHIDAEAGGVLDIAEVVKAHPANTHFYCCGPGPMLSAFEAATAHLPAAQVHVEYFTAKQEAALDGGYTVELK
RSGKSLQVPGGKTILQVLKDAGVEVAHSCEEGVCGACETRVLSGIPDHRDAILSEPEKAANNTMMICCSGCKGERLVLDL
>Mature_320_residues
MNDLPQFTGALLELRLESIQFLARDTLLYAFASPVGAALPPCEPGSHIGLHLPNGLVRQYSLLEGGREPRRYQVGVKRDP
KSRGGSSFMHEQLRPGAVIQVEPPRNNFPLNEEAEHSVLVGGGIGITPILCMARRLKALGRSMTLVYSCRDRVDVAFLEE
LRSYENLHLHIDAEAGGVLDIAEVVKAHPANTHFYCCGPGPMLSAFEAATAHLPAAQVHVEYFTAKQEAALDGGYTVELK
RSGKSLQVPGGKTILQVLKDAGVEVAHSCEEGVCGACETRVLSGIPDHRDAILSEPEKAANNTMMICCSGCKGERLVLDL

Specific function: Degrades exclusively diarylether compounds having carboxyl groups in the 3- or 4-position. Yields a hemiacetal that spontaneously hydrolyzes to phenol and protocatechuate [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=319, Percent_Identity=35.1097178683386, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787098, Length=210, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 34558; Mature: 34558

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLPQFTGALLELRLESIQFLARDTLLYAFASPVGAALPPCEPGSHIGLHLPNGLVRQY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHH
SLLEGGREPRRYQVGVKRDPKSRGGSSFMHEQLRPGAVIQVEPPRNNFPLNEEAEHSVLV
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEE
GGGIGITPILCMARRLKALGRSMTLVYSCRDRVDVAFLEELRSYENLHLHIDAEAGGVLD
ECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCEEH
IAEVVKAHPANTHFYCCGPGPMLSAFEAATAHLPAAQVHVEYFTAKQEAALDGGYTVELK
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHEEEEEEHHHHHHHCCCCEEEEEE
RSGKSLQVPGGKTILQVLKDAGVEVAHSCEEGVCGACETRVLSGIPDHRDAILSEPEKAA
CCCCEEECCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHCCCHHHC
NNTMMICCSGCKGERLVLDL
CCEEEEEECCCCCCEEEEEC
>Mature Secondary Structure
MNDLPQFTGALLELRLESIQFLARDTLLYAFASPVGAALPPCEPGSHIGLHLPNGLVRQY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHH
SLLEGGREPRRYQVGVKRDPKSRGGSSFMHEQLRPGAVIQVEPPRNNFPLNEEAEHSVLV
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEE
GGGIGITPILCMARRLKALGRSMTLVYSCRDRVDVAFLEELRSYENLHLHIDAEAGGVLD
ECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCEEH
IAEVVKAHPANTHFYCCGPGPMLSAFEAATAHLPAAQVHVEYFTAKQEAALDGGYTVELK
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHEEEEEEHHHHHHHCCCCEEEEEE
RSGKSLQVPGGKTILQVLKDAGVEVAHSCEEGVCGACETRVLSGIPDHRDAILSEPEKAA
CCCCEEECCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHCCCHHHC
NNTMMICCSGCKGERLVLDL
CCEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7710319 [H]