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The map label for this gene is htrB [H]

Identifier: 73539546

GI number: 73539546

Start: 2555644

End: 2556525

Strand: Direct

Name: htrB [H]

Synonym: Reut_B5725

Alternate gene names: 73539546

Gene position: 2555644-2556525 (Clockwise)

Preceding gene: 73539543

Following gene: 73539548

Centisome position: 93.75

GC content: 60.54

Gene sequence:

>882_bases
ATGATCGACAGCCTCATCGCGGCACTGCTCACCCTGCTGCTCAAGGCCTTCGCCGCGCTGCCGTATCGATGGTCCGCCCA
CATTGGCGCAGCACTTGGCGCGCTGCTTTACAGCATCCCGAGTCGCAGGCGGAACATTGTCCATACCAACCTGTCGCTGT
GTTTCCCCGATCTGTCCGATGCTGCACGGCGCGACCTGGCACGGCTGCATTTTCGCCACGTCATGCGCAGCTACGTCGAG
CGAGGCGTGCAATGGTTCGGCGACCGCGATCGCCTCAATGGCCTGCTGCGATTGGAATCCGAGATAGACCTGTCGTCGTG
CTCGCGTACTCCGACGATTTTTCTCGGCTTCCACTTCGTCGCCATCGAAGCCGGCTGCATGTTCTATTCGATGGCCGGCA
GCCCGGTAACGTCGCTTTACACCAGAATGTCGAACCGGGCGCTCGAAACCATTGCCAGAACGCAGCGCGAGCGATTTGGC
GCGGTCATGGTGCCGCGTAACGAAAGCGCAAGGCAGGTCATTCAGTCATTGCGCAAGGGTGTACCGGTCATGCTTGCCGC
CGACATGGACTTCGGCCTGAAAGACTCCGTATTCGTTCCGTTCTTCGGCGTCGCGGCCTGCACACTGATCTCCGTGTCCC
GCCTCGCCAGGCTCACAGGTGCCCAGGTCGTGCCTTTTACAACCGAGGTTTTGCCCGACTATCGCGGCTACCAACTCCGG
GTGTTCAAGCCGCTACCAGGCTTTCCGTCGGCCTGCGTAACGGCAGACGCGGCACAGATGAACGCGTTTCTCGAAGCGCA
AATCCTGCGAATTCCCGAGCAGTACTATTGGGTGCATCGCCGGTTCAAGAATCGGCCCTCGGGGGAACCGGCGATCTATT
GA

Upstream 100 bases:

>100_bases
TATGTCCCGCCGCAGGGAATCGGGCCGGCGTGCTACAGTGCAGGTACATCAATGCTAGTCGTCGGGCCAGTGTGCCAGCG
ACTTCCGCTATCCCCCCCAC

Downstream 100 bases:

>100_bases
GCCGCGCTCGATCTGATCCTCCCGTCCATACGACACGATGGCGCAGGCAGAGTGCCGGTGCTCGGCAAACTCGAAGCGTT
CATCGTCTACCTGGACAACA

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MIDSLIAALLTLLLKAFAALPYRWSAHIGAALGALLYSIPSRRRNIVHTNLSLCFPDLSDAARRDLARLHFRHVMRSYVE
RGVQWFGDRDRLNGLLRLESEIDLSSCSRTPTIFLGFHFVAIEAGCMFYSMAGSPVTSLYTRMSNRALETIARTQRERFG
AVMVPRNESARQVIQSLRKGVPVMLAADMDFGLKDSVFVPFFGVAACTLISVSRLARLTGAQVVPFTTEVLPDYRGYQLR
VFKPLPGFPSACVTADAAQMNAFLEAQILRIPEQYYWVHRRFKNRPSGEPAIY

Sequences:

>Translated_293_residues
MIDSLIAALLTLLLKAFAALPYRWSAHIGAALGALLYSIPSRRRNIVHTNLSLCFPDLSDAARRDLARLHFRHVMRSYVE
RGVQWFGDRDRLNGLLRLESEIDLSSCSRTPTIFLGFHFVAIEAGCMFYSMAGSPVTSLYTRMSNRALETIARTQRERFG
AVMVPRNESARQVIQSLRKGVPVMLAADMDFGLKDSVFVPFFGVAACTLISVSRLARLTGAQVVPFTTEVLPDYRGYQLR
VFKPLPGFPSACVTADAAQMNAFLEAQILRIPEQYYWVHRRFKNRPSGEPAIY
>Mature_293_residues
MIDSLIAALLTLLLKAFAALPYRWSAHIGAALGALLYSIPSRRRNIVHTNLSLCFPDLSDAARRDLARLHFRHVMRSYVE
RGVQWFGDRDRLNGLLRLESEIDLSSCSRTPTIFLGFHFVAIEAGCMFYSMAGSPVTSLYTRMSNRALETIARTQRERFG
AVMVPRNESARQVIQSLRKGVPVMLAADMDFGLKDSVFVPFFGVAACTLISVSRLARLTGAQVVPFTTEVLPDYRGYQLR
VFKPLPGFPSACVTADAAQMNAFLEAQILRIPEQYYWVHRRFKNRPSGEPAIY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=278, Percent_Identity=36.3309352517986, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87082094, Length=284, Percent_Identity=33.4507042253521, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788161, Length=274, Percent_Identity=30.2919708029197, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 32942; Mature: 32942

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSLIAALLTLLLKAFAALPYRWSAHIGAALGALLYSIPSRRRNIVHTNLSLCFPDLSD
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCEEECCHHHCCCCHHH
AARRDLARLHFRHVMRSYVERGVQWFGDRDRLNGLLRLESEIDLSSCSRTPTIFLGFHFV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHEEECCCCCHHHCCCCCEEEEHHHHH
AIEAGCMFYSMAGSPVTSLYTRMSNRALETIARTQRERFGAVMVPRNESARQVIQSLRKG
HHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCC
VPVMLAADMDFGLKDSVFVPFFGVAACTLISVSRLARLTGAQVVPFTTEVLPDYRGYQLR
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCCCCEEE
VFKPLPGFPSACVTADAAQMNAFLEAQILRIPEQYYWVHRRFKNRPSGEPAIY
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIDSLIAALLTLLLKAFAALPYRWSAHIGAALGALLYSIPSRRRNIVHTNLSLCFPDLSD
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCEEECCHHHCCCCHHH
AARRDLARLHFRHVMRSYVERGVQWFGDRDRLNGLLRLESEIDLSSCSRTPTIFLGFHFV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHEEECCCCCHHHCCCCCEEEEHHHHH
AIEAGCMFYSMAGSPVTSLYTRMSNRALETIARTQRERFGAVMVPRNESARQVIQSLRKG
HHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCC
VPVMLAADMDFGLKDSVFVPFFGVAACTLISVSRLARLTGAQVVPFTTEVLPDYRGYQLR
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCCCCEEE
VFKPLPGFPSACVTADAAQMNAFLEAQILRIPEQYYWVHRRFKNRPSGEPAIY
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]