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The map label for this gene is tfdEI [H]

Identifier: 73539399

GI number: 73539399

Start: 2394764

End: 2395477

Strand: Reverse

Name: tfdEI [H]

Synonym: Reut_B5577

Alternate gene names: 73539399

Gene position: 2395477-2394764 (Counterclockwise)

Preceding gene: 73539400

Following gene: 73539398

Centisome position: 87.87

GC content: 68.49

Gene sequence:

>714_bases
ATGCCAGCCACTCCCAACAGCAAATGGATCCGTGTCGACAGCGACGCCGGCAGCTTTGACGCCTACCTGAGCCTGCCGCC
TGCAGGCGTGCAGCCCGATGGCCCCGCCATCGTGCTGCTGCAGGAAATCTTCGGCGTCAACGAACATATCCGCGCGGTAG
CCGACCAGTACGCGGCCGACGGCTACACCGTGCTCGCGCCCGATGTGTTCTGGCGCGATGCGCCGCGCATCGAGCTGGGC
TATGCCGGCCCCGACATGGAGCGCGCCATGGCGCTGCGCAAGTCGGTCGACGTGGAAGCCGCCGTGCGCGATATCGCCGC
GACCGTGCGCACGTTGCGCACGCACACCGGTGCAGGCAGCAAGGTTGCGGCGGTTGGCTATTGCTTTGGCGGCCTGCTCG
CCTACCTGAGCGCGGCGCGCGGCCTCGTCAACGCCGCGGTGCCCTACTACGGCGGCGGCATCCAGAACAACCTGCAGGAA
GCGAGCGCGCTGAACGTGCCGGTGCAGTTCCACTACGGCGCGCTCGACGCGCATATCACGCCCGATGCGGTCGCCAACGT
GCGTGCCGCTGTCGCCGGCAAGCGTGGCACCGAGGTGTTCGTCTACGACGGCGCCGACCACGGATTCAACTGCTGGGCCC
GCGCGAGTTACCACCAGCACAGCGCAGCGCTGGCGCACGGCCGCGCACTGACCTTCCTCGCGCAGACGCTGTAA

Upstream 100 bases:

>100_bases
TCCAGCAGTCGTCCGCGTACTGAGCCGTGGCCGGCGTGATCTGGCCGGCGGTGGCACACTTGCGGTTTGCCCATCCTTCA
TCCGTCACCAGGAACCCGCC

Downstream 100 bases:

>100_bases
TCCCGCGCCCCTCGCCGGAGGGGCCCGCCTTGCCACCGGCGTGCGGCACGCCGTATGCTGAGCTGTCGAGCTTCAAGACT
TGCTGGATCCAAACCACGAT

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Carboxymethylenebutenolidase I; Dienelactone hydrolase I; DLH I [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MPATPNSKWIRVDSDAGSFDAYLSLPPAGVQPDGPAIVLLQEIFGVNEHIRAVADQYAADGYTVLAPDVFWRDAPRIELG
YAGPDMERAMALRKSVDVEAAVRDIAATVRTLRTHTGAGSKVAAVGYCFGGLLAYLSAARGLVNAAVPYYGGGIQNNLQE
ASALNVPVQFHYGALDAHITPDAVANVRAAVAGKRGTEVFVYDGADHGFNCWARASYHQHSAALAHGRALTFLAQTL

Sequences:

>Translated_237_residues
MPATPNSKWIRVDSDAGSFDAYLSLPPAGVQPDGPAIVLLQEIFGVNEHIRAVADQYAADGYTVLAPDVFWRDAPRIELG
YAGPDMERAMALRKSVDVEAAVRDIAATVRTLRTHTGAGSKVAAVGYCFGGLLAYLSAARGLVNAAVPYYGGGIQNNLQE
ASALNVPVQFHYGALDAHITPDAVANVRAAVAGKRGTEVFVYDGADHGFNCWARASYHQHSAALAHGRALTFLAQTL
>Mature_236_residues
PATPNSKWIRVDSDAGSFDAYLSLPPAGVQPDGPAIVLLQEIFGVNEHIRAVADQYAADGYTVLAPDVFWRDAPRIELGY
AGPDMERAMALRKSVDVEAAVRDIAATVRTLRTHTGAGSKVAAVGYCFGGLLAYLSAARGLVNAAVPYYGGGIQNNLQEA
SALNVPVQFHYGALDAHITPDAVANVRAAVAGKRGTEVFVYDGADHGFNCWARASYHQHSAALAHGRALTFLAQTL

Specific function: Ring cleavage of cyclic ester dienelactone to produce maleylacetate [H]

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=237, Percent_Identity=29.1139240506329, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 25108; Mature: 24977

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATPNSKWIRVDSDAGSFDAYLSLPPAGVQPDGPAIVLLQEIFGVNEHIRAVADQYAAD
CCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHCC
GYTVLAPDVFWRDAPRIELGYAGPDMERAMALRKSVDVEAAVRDIAATVRTLRTHTGAGS
CEEEECCHHHCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
KVAAVGYCFGGLLAYLSAARGLVNAAVPYYGGGIQNNLQEASALNVPVQFHYGALDAHIT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCEECCCC
PDAVANVRAAVAGKRGTEVFVYDGADHGFNCWARASYHQHSAALAHGRALTFLAQTL
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
PATPNSKWIRVDSDAGSFDAYLSLPPAGVQPDGPAIVLLQEIFGVNEHIRAVADQYAAD
CCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCEEEEEHHHHCCCHHHHHHHHHHHCC
GYTVLAPDVFWRDAPRIELGYAGPDMERAMALRKSVDVEAAVRDIAATVRTLRTHTGAGS
CEEEECCHHHCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
KVAAVGYCFGGLLAYLSAARGLVNAAVPYYGGGIQNNLQEASALNVPVQFHYGALDAHIT
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCEECCCC
PDAVANVRAAVAGKRGTEVFVYDGADHGFNCWARASYHQHSAALAHGRALTFLAQTL
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185214 [H]