Click here to switch to the map view.

The map label for this gene is cobA [H]

Identifier: 73538844

GI number: 73538844

Start: 1751243

End: 1752028

Strand: Direct

Name: cobA [H]

Synonym: Reut_B5019

Alternate gene names: 73538844

Gene position: 1751243-1752028 (Clockwise)

Preceding gene: 73538843

Following gene: 73538846

Centisome position: 64.24

GC content: 70.23

Gene sequence:

>786_bases
ATGAACCCTCCCCTCAAGCCCGGCAAGGTATGGCTGGTCGGCGCCGGCCCCGGCAGCCCCGAACTCGTCACGGTGCGCGC
GGCACGCGTGCTGGAGCGCGCGCAGGTCTGGCTCGTGGATGACCTGGTGTCGCCGGAGATGACCTGCTATGCCAGCCCGG
GCACGCACGTGGAATGGGTCGGCAAGCGCGGCGGACGCTGCTCGGTGAGCCAGGACCGCATCCTGCAACTGACGCTGATG
CATGCCATGGCCGGCAAGGAGGTCGCGCGCGTGAAGGGCGGCGATCCGCTGCTGTTCGGGCGCGGCGCCGAGGAATCGGC
ATTTCTGCGCGCGCATGGTGTGCCGGTCGAGGTCGTCAATGGCATCAGCAGCGGACAGGCCGCCGCGCAGGCGCTGGGCG
TGGCGCTGACGCACCGCGCGCACTGCCATGGCGTGAGTTTAGTGACCGCGCACACGAGTGATCACGGCAGCCCTGACTGG
GGCGCGCTGGCGCGCAGCGGCACGACGCTGGTGATCTACATGGGTATGAGCCGGCTCGCGGCAATCCGCGATGCGCTGCT
GGCGGCAAACATGCTGCCCGGCACGCTCGCGGCAGTCGTCATGCACGCAGGCGGCAACGGGCAGCGGTGCTGGACCGGCA
CGCTTGCCACGCTGGGCGAAGCGCTCGACGCGGGACTGGCCAGTCCCGCGGTGATCATGGTCGGCGCGGTGCTGTCCGAC
GCATTGGCGCCGGTGCATGCGCCGGTGCATGAAGAGCCAGCGCCTCAGTACGGCAGCCCGACGTAA

Upstream 100 bases:

>100_bases
GATGGACAAGCCCATCGGCAAGTACAACGTATTCAACAAGACCACTCGCTCGTCCGGCACCAGCCACTGACGCTCGCCAC
GAAAGATAAATGGACTCCCC

Downstream 100 bases:

>100_bases
TTCTCGGCCAGCGCCTTGCAGGCGGCTTCGGAGCTGACCAGGAAATCAAGCTCGGCTTGCTGGATGCGGGCCGAGAACGC
GTCGGCATCGGGGAACCGGT

Product: uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNPPLKPGKVWLVGAGPGSPELVTVRAARVLERAQVWLVDDLVSPEMTCYASPGTHVEWVGKRGGRCSVSQDRILQLTLM
HAMAGKEVARVKGGDPLLFGRGAEESAFLRAHGVPVEVVNGISSGQAAAQALGVALTHRAHCHGVSLVTAHTSDHGSPDW
GALARSGTTLVIYMGMSRLAAIRDALLAANMLPGTLAAVVMHAGGNGQRCWTGTLATLGEALDAGLASPAVIMVGAVLSD
ALAPVHAPVHEEPAPQYGSPT

Sequences:

>Translated_261_residues
MNPPLKPGKVWLVGAGPGSPELVTVRAARVLERAQVWLVDDLVSPEMTCYASPGTHVEWVGKRGGRCSVSQDRILQLTLM
HAMAGKEVARVKGGDPLLFGRGAEESAFLRAHGVPVEVVNGISSGQAAAQALGVALTHRAHCHGVSLVTAHTSDHGSPDW
GALARSGTTLVIYMGMSRLAAIRDALLAANMLPGTLAAVVMHAGGNGQRCWTGTLATLGEALDAGLASPAVIMVGAVLSD
ALAPVHAPVHEEPAPQYGSPT
>Mature_261_residues
MNPPLKPGKVWLVGAGPGSPELVTVRAARVLERAQVWLVDDLVSPEMTCYASPGTHVEWVGKRGGRCSVSQDRILQLTLM
HAMAGKEVARVKGGDPLLFGRGAEESAFLRAHGVPVEVVNGISSGQAAAQALGVALTHRAHCHGVSLVTAHTSDHGSPDW
GALARSGTTLVIYMGMSRLAAIRDALLAANMLPGTLAAVVMHAGGNGQRCWTGTLATLGEALDAGLASPAVIMVGAVLSD
ALAPVHAPVHEEPAPQYGSPT

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=246, Percent_Identity=39.8373983739837, Blast_Score=155, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=31.0924369747899, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26911; Mature: 26911

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPLKPGKVWLVGAGPGSPELVTVRAARVLERAQVWLVDDLVSPEMTCYASPGTHVEWV
CCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHEEEEHHHCCCCEEEEECCCCCEEEE
GKRGGRCSVSQDRILQLTLMHAMAGKEVARVKGGDPLLFGRGAEESAFLRAHGVPVEVVN
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCEEEEECCCCHHHHC
GISSGQAAAQALGVALTHRAHCHGVSLVTAHTSDHGSPDWGALARSGTTLVIYMGMSRLA
CCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEECHHHHH
AIRDALLAANMLPGTLAAVVMHAGGNGQRCWTGTLATLGEALDAGLASPAVIMVGAVLSD
HHHHHHHHHHCCCHHHHHHEEECCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ALAPVHAPVHEEPAPQYGSPT
HHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPPLKPGKVWLVGAGPGSPELVTVRAARVLERAQVWLVDDLVSPEMTCYASPGTHVEWV
CCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHEEEEHHHCCCCEEEEECCCCCEEEE
GKRGGRCSVSQDRILQLTLMHAMAGKEVARVKGGDPLLFGRGAEESAFLRAHGVPVEVVN
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCEEEEECCCCHHHHC
GISSGQAAAQALGVALTHRAHCHGVSLVTAHTSDHGSPDWGALARSGTTLVIYMGMSRLA
CCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEECHHHHH
AIRDALLAANMLPGTLAAVVMHAGGNGQRCWTGTLATLGEALDAGLASPAVIMVGAVLSD
HHHHHHHHHHCCCHHHHHHEEECCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ALAPVHAPVHEEPAPQYGSPT
HHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]