Click here to switch to the map view.

The map label for this gene is cynT [H]

Identifier: 73538832

GI number: 73538832

Start: 1741182

End: 1741874

Strand: Direct

Name: cynT [H]

Synonym: Reut_B5007

Alternate gene names: 73538832

Gene position: 1741182-1741874 (Clockwise)

Preceding gene: 73538831

Following gene: 73538833

Centisome position: 63.87

GC content: 64.94

Gene sequence:

>693_bases
ATGCACGAGATCGAACGTCTGCTGAAAGGGTTCGAGCGCTTCCAGCAGCATTACTTCGAGGACGAACCCGAGCTTTTCGA
TACGCTGCGCGACGGCCAGCGTCCGCCGACGCTGCTGATCGGCTGCAGTGATTCGCGTGTCGACCCCGCCCTGCTACTTG
GATGCGACCCCGGAGAGCTGTTTACGGTACGCAATATCGGCAACCTGGTGCCGCCATGCACCGGCAACCACGAAGGCAGC
CTGCACGGCGTATCCGCCGCCATCCAGTTCGCGGTGGAGCAGTTGCGCGTGGCCCGCATCATCGTGATGGGCCATGCCGG
ATGCGGCGGGATTCGCGCATTACTGGCGCAACCCGCGGGTGCCGAACACGAAGACGCTGTCGCGGGCAGAGACTTCATCG
GGCCGTGGGTGCGGATCGCCAGTTCCGCGAGGCGGCACGTCGACGACACACTGGCCGGCGCGAGCAGCGCGCAACGCCAG
CGCGCCTGTGAACAGGCAGCGATTCTCGTGTCGCTAGGCAATCTCGAGACGTTTCCTTTCGTGCGACGCGAGCTGGACCG
CGGCCGGCTGACGCTGCACGGCTGGTATTTCGACCTCGAAGCAGGTGCGCTGCTCGCGTACTCGCATCGGGCCGACAGCT
TCCTGCCACTCGTATGCCCGATCGGGCGTGGCAACTCATCCGCGAAGAACTGA

Upstream 100 bases:

>100_bases
CAGCCTGCTGATCGGCAGGGCCAGCATCACGGGTATCGAGCTCGAAGGCGCCGACAGCCCGCTGGTGCAGTAGGGAAAAC
CGTCCCCGGAGGCTTCTGGC

Downstream 100 bases:

>100_bases
GGATCATGCAAGGATTCGTTATCGGCATCGCCGGCACGTCTGGCAGCGGCAAGACCACACTTATTACCGCCATGCTGCCC
TGGTTCCGTTCACACGGCCT

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MHEIERLLKGFERFQQHYFEDEPELFDTLRDGQRPPTLLIGCSDSRVDPALLLGCDPGELFTVRNIGNLVPPCTGNHEGS
LHGVSAAIQFAVEQLRVARIIVMGHAGCGGIRALLAQPAGAEHEDAVAGRDFIGPWVRIASSARRHVDDTLAGASSAQRQ
RACEQAAILVSLGNLETFPFVRRELDRGRLTLHGWYFDLEAGALLAYSHRADSFLPLVCPIGRGNSSAKN

Sequences:

>Translated_230_residues
MHEIERLLKGFERFQQHYFEDEPELFDTLRDGQRPPTLLIGCSDSRVDPALLLGCDPGELFTVRNIGNLVPPCTGNHEGS
LHGVSAAIQFAVEQLRVARIIVMGHAGCGGIRALLAQPAGAEHEDAVAGRDFIGPWVRIASSARRHVDDTLAGASSAQRQ
RACEQAAILVSLGNLETFPFVRRELDRGRLTLHGWYFDLEAGALLAYSHRADSFLPLVCPIGRGNSSAKN
>Mature_230_residues
MHEIERLLKGFERFQQHYFEDEPELFDTLRDGQRPPTLLIGCSDSRVDPALLLGCDPGELFTVRNIGNLVPPCTGNHEGS
LHGVSAAIQFAVEQLRVARIIVMGHAGCGGIRALLAQPAGAEHEDAVAGRDFIGPWVRIASSARRHVDDTLAGASSAQRQ
RACEQAAILVSLGNLETFPFVRRELDRGRLTLHGWYFDLEAGALLAYSHRADSFLPLVCPIGRGNSSAKN

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=214, Percent_Identity=37.3831775700935, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1786318, Length=208, Percent_Identity=31.25, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI115532990, Length=240, Percent_Identity=24.5833333333333, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324292, Length=199, Percent_Identity=29.6482412060301, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 25105; Mature: 25105

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEIERLLKGFERFQQHYFEDEPELFDTLRDGQRPPTLLIGCSDSRVDPALLLGCDPGEL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCE
FTVRNIGNLVPPCTGNHEGSLHGVSAAIQFAVEQLRVARIIVMGHAGCGGIRALLAQPAG
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCC
AEHEDAVAGRDFIGPWVRIASSARRHVDDTLAGASSAQRQRACEQAAILVSLGNLETFPF
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCHHHH
VRRELDRGRLTLHGWYFDLEAGALLAYSHRADSFLPLVCPIGRGNSSAKN
HHHHHCCCEEEEEEEEEEECCCEEEEECCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MHEIERLLKGFERFQQHYFEDEPELFDTLRDGQRPPTLLIGCSDSRVDPALLLGCDPGEL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCE
FTVRNIGNLVPPCTGNHEGSLHGVSAAIQFAVEQLRVARIIVMGHAGCGGIRALLAQPAG
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCC
AEHEDAVAGRDFIGPWVRIASSARRHVDDTLAGASSAQRQRACEQAAILVSLGNLETFPF
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCHHHH
VRRELDRGRLTLHGWYFDLEAGALLAYSHRADSFLPLVCPIGRGNSSAKN
HHHHHCCCEEEEEEEEEEECCCEEEEECCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]