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The map label for this gene is narH [H]

Identifier: 73538828

GI number: 73538828

Start: 1737428

End: 1738975

Strand: Direct

Name: narH [H]

Synonym: Reut_B5003

Alternate gene names: 73538828

Gene position: 1737428-1738975 (Clockwise)

Preceding gene: 73538827

Following gene: 73538829

Centisome position: 63.73

GC content: 63.24

Gene sequence:

>1548_bases
ATGAAGATCCGTGCCCAGGTAGCCATGGTGCTGAACCTCGACAAGTGCATCGGCTGCCATACCTGCTCCGTAACCTGCAA
GAACGTGTGGACCAGCCGCGACGGCATCGAGTACGCATGGTTCAACAATGTCGAAACCAAGCCGGGTATCGGTTACCCCA
AGGAATGGGAGAACCAGGACAAATGGCAAGGCGGCTGGAAGCGTAACGCCAATGGCAGCCTTGAGCCGCGCCAGGGCGGC
AAGCTGAAGATCCTGGCGAACCTGTTCGCCAATCCGAACCTGCCGCAGATCGACGACTACTACGAGCCGTTCACGTACGA
CTACGAGCACCTGCAGAAGGCGCCGCTCGTGCAGACGCCGCCGACCGCGCGTCCTGTGTCTGTGCTGACCGGCCGCAAGA
TGGAAAAGATCGAATGGGGTCCGAACTGGGAAGACGATCTCGGCGGCGAATTCAGCTCGCGCGGCCGCGACAAGCTGTTC
GACGACGTGCAGAAGGAGATGTACTCGACCTTCGAGAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCT
GAACCCGGCATGCGTGGCATCTTGCCCGTCCGGCTCGATCTACAAGCGCGAGGATGACGGCATCGTACTCGTCGACCAGG
ACAAGTGCCGGGGCTGGCGCATGTGCATCTCGGGCTGCCCGTACAAGAAGATCTACTTCAACTGGCAGAGCGGCAAGGCC
GAGAAGTGCCTGTTCTGCTATCCGCGCATCGAGGCCGGCCAGCCGACCGTGTGCTCGGAAACCTGCGTGGGCCGCATCCG
CTATCTCGGCGTGATGCTGTATGACGCCGATCGTATCGAGCAAGCCGCGTCGGTCGCTGATGAGCGAGACCTGTACCAGT
CGCAGCTCGACGTGTTCCTCGACCCGCACGATCCCATCGTTCAGGCCGAGGCGCGCCGCCAGGGCATTCCGCAGAGCTGG
CTCGACGCCGCGCGCAAGTCGCCGGTCTACAAGATGGCCTGCGAATGGAAGGTCGCCTTCCCGCTGCACCCCGAGTACCG
CACGCTGCCGATGGTCTGGTACATCCCGCCGCTGTCGCCGATCCAGTCCGCCGCCGAGGCGGGCCATATGGGCATGAACG
GCGTGATCCCGGACGTGAAGAGCCTGCGCATTCCGGTGAAGTACCTCGCCAACCTGCTGACCGCGGGCGATGTCATGCCC
GTTCTGTCCGCGCTCGACCGCATGCTCGCGATGCGCGCCTACAAGCGTTCGCAGGTGGTGGATGGCTTGCAGGACCTGGC
GGTGCTCGAGCAGGTCGGCCTGACGCCGGCGCAGGTCGAAGACATGTACCAGACCATGGCCATCGCAAACTATGAAGACC
GCTTCGTCATCCCGTCGTCGCACAAGGAAATGGTCGAGGACAGCTTCGGCGACAAGGCCAGTTGCGGCTTCACCTTCGGC
AACGGCTGCTCCGGCGGCACGTCCGAAGGCACGCTGTTCGGCAAGAAGCCGAAGCCGGCCATCGCTTATGCCGCGCTGCC
GCGTTCGCGCAAGGCATTCGCCGGCTGA

Upstream 100 bases:

>100_bases
GCAGCAACCGCGACGAGTTCGTGATCCTGCGCAAGATGAAGAAGGTCGACTGGCTGGAAGGGCCGCTCGTGGAAACGACA
GTGAAGGAAGGAGCCTGAGA

Downstream 100 bases:

>100_bases
CCAGACAGGAGAACCAACATGCCCCTCTACCCAGTTCTTAGCGCCCTGCTCTGCTACCCCGACGAAGCACTGCTCGACAG
CCTGCCCGAGATAGACGCGG

Product: respiratory nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKEWENQDKWQGGWKRNANGSLEPRQGG
KLKILANLFANPNLPQIDDYYEPFTYDYEHLQKAPLVQTPPTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLF
DDVQKEMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPIVQAEARRQGIPQSW
LDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMP
VLSALDRMLAMRAYKRSQVVDGLQDLAVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFGDKASCGFTFG
NGCSGGTSEGTLFGKKPKPAIAYAALPRSRKAFAG

Sequences:

>Translated_515_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKEWENQDKWQGGWKRNANGSLEPRQGG
KLKILANLFANPNLPQIDDYYEPFTYDYEHLQKAPLVQTPPTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLF
DDVQKEMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPIVQAEARRQGIPQSW
LDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMP
VLSALDRMLAMRAYKRSQVVDGLQDLAVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFGDKASCGFTFG
NGCSGGTSEGTLFGKKPKPAIAYAALPRSRKAFAG
>Mature_515_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKEWENQDKWQGGWKRNANGSLEPRQGG
KLKILANLFANPNLPQIDDYYEPFTYDYEHLQKAPLVQTPPTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLF
DDVQKEMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPIVQAEARRQGIPQSW
LDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMP
VLSALDRMLAMRAYKRSQVVDGLQDLAVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFGDKASCGFTFG
NGCSGGTSEGTLFGKKPKPAIAYAALPRSRKAFAG

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=502, Percent_Identity=71.1155378486056, Blast_Score=782, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=502, Percent_Identity=69.9203187250996, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=180, Percent_Identity=32.7777777777778, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787122, Length=150, Percent_Identity=34, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787749, Length=102, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI2367345, Length=101, Percent_Identity=31.6831683168317, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=35.5555555555556, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=35.0515463917526, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57933; Mature: 57933

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKEWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNANGSLEPRQGGKLKILANLFANPNLPQIDDYYEPFTYDYEHLQKAPLVQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLFDDVQKEMYSTFENTFMMYLP
CCCCCHHHHCCCCHHCCCCCCCCCHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHEEE
DPHDPIVQAEARRQGIPQSWLDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCHH
IQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMPVLSALDRMLAMRAYKRSQVV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
DGLQDLAVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFGDKASCGFTFG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCCEEC
NGCSGGTSEGTLFGKKPKPAIAYAALPRSRKAFAG
CCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKEWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNANGSLEPRQGGKLKILANLFANPNLPQIDDYYEPFTYDYEHLQKAPLVQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLFDDVQKEMYSTFENTFMMYLP
CCCCCHHHHCCCCHHCCCCCCCCCHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHEEE
DPHDPIVQAEARRQGIPQSWLDAARKSPVYKMACEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCHH
IQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMPVLSALDRMLAMRAYKRSQVV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
DGLQDLAVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFGDKASCGFTFG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCCEEC
NGCSGGTSEGTLFGKKPKPAIAYAALPRSRKAFAG
CCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]