| Definition | Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence. |
|---|---|
| Accession | NC_007348 |
| Length | 2,726,152 |
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The map label for this gene is napA [H]
Identifier: 73538588
GI number: 73538588
Start: 1463288
End: 1465783
Strand: Direct
Name: napA [H]
Synonym: Reut_B4763
Alternate gene names: 73538588
Gene position: 1463288-1465783 (Clockwise)
Preceding gene: 73538587
Following gene: 73538589
Centisome position: 53.68
GC content: 62.9
Gene sequence:
>2496_bases ATGAAAGTCTCTCGTCGGGATTTCATTAAACAGACTGCCATCGCAGCGACAGCATCGGTTGCTGGCATTCCGCTTGGCAC CGAGGCCGCCAACTTCGTCACCGACAGCGAAGTCACGAAGCTGAAGTGGTCGAAGGCGCCCTGCCGCTTCTGCGGCACGG GCTGCGGCGTCACCGTGGCCGTGCGAGACAACAAGGTGGTGGCCACGCAGGGCGATCCGCAGTGCGAGGTCAACAAGGGC CTGAACTGCGTCAAGGGCTACTTCCTGTCGAAGATCATGTACGGCCAGGACCGGCTCACCAAGCCGCTGCTGCGCATGAA GAACGGCAAGTACGACAAGAACGGCGAGTTCGCGCCGGTCACGTGGGACCAGGCGTTCGACGAGATGGAGCGCCAGTTCA AGCGCGTGCTCAAGGAGAAAGGCCCGACCGCGGTGGGCATGTTCGGCTCCGGCCAGTGGACCGTTTGGGAAGGCTACGCG GCGTCCAAGCTCTACAAGGCGGGCTTCCGTTCCAACAATATCGACCCGAACGCACGCCATTGCATGGCGTCTGCAGTGCA GGGCTTCATGCGCACCTTCGGCATGGACGAGCCGATGGGCTGCTATGACGATTTCGAGGCCGCCGACGCGTTCGTGCTGT GGGGCTCGAACATGGCGGAGATGCACCCGATCCTGTGGACGCGCATCACGGACCGGCGCCTGAGCCACCCGAAGACGCGC GTGGCGGTGCTGTCCACTTTCACGCACCGCTCGTTCGACCTGGCGGATATCCCGATCATCTTCACGCCGCAGACCGACCT GGCGATGCTGAACTACATCGCCAACTACATCATCCAGAACAACAAGGTCAACAAGGACTTCGTCAACAAACACACGGTGT TCAAGGAAGGCGTGACGGAAATCGGCTACGGCTTGCGGCCTGACCATCCGCTGCAGAAGGCCGCAAAGAACGCGGCCAAT CCGGGCGATTCGAAGCCCATCACGTTTGATGATTTCGCCAAGTTCGTCTCCAAGTACGACGCCGACTACGTCAGCAAGCT CTCCGGCGTACCGAAGGACAAGCTCAGGCAGCTTGCCGAGCTGTACGCGGACCCCAACGTCAAGGTGATGTCGTTGTGGA CCATGGGGTTCAACCAGCATACGCGTGGCAGCTGGGTCAACAACATGGTCTACAACGTCCACCTGCTGACCGGCAAGATC GCCACGCCGGGCAACAGCCCGTTCTCGCTGACCGGGCAGCCGTCCGCGTGCGGCACCGCGCGCGAAGTCGGCACGTTCTC GCACCGGCTCCCAGCCGACATGGTGGTCACGAATCCGAAGCACCGCGAGGAAGCCGAGCGCATCTGGAAACTGCCACCAG GCACCATCGTCGAGAAGCCCGGCTACCACGCCGTGCTGCAGAACCGCATGCTCCGGGACGGCAAGCTCAATGCGTACTGG GTGCAGGTCAACAACAATATGCAGGCGGCGGCCAACATCATGGAGGAAGCCCTGCCGGGCTACCGCAACCCCGCCAACTT CATCGTTGTTTCGGACGCATACCCGACCGTGACAGCGCTGTCGGCGGACCTGATCCTGCCCAGCGCGATGTGGGTGGAAA AGGAAGGCGCCTACGGTAACGCGGAACGCCGCACGCAGTTCTGGCACCAGCTTGTCGATGCGCCGGGCGATGCGCGCTCC GACCTGTGGCAGCTCATGGAGTTTTCCAAGCGCTTCAAGGTCGAGGAAGTCTGGCCGGCCGAACTCGTGGCAAAGAAACC TGAATACAAGGGCAAGACGCTGTTCGACGTGCTGTACCGCAACGGCCAGGTCGACAAGTTCCCGATCAAGGAAGTCAGCA CCGAATACCACAATGCCGAGGCCCAGGCCTTCGGCTTCTACGTGCAGAAGGGCCTGTTCGAGGAATACGCGAGCTTCGGC CGCGGGCATGGCCACGACCTGGCACCGTTCGACCGCTACCACGAGGAACGCGGCCTGCGCTGGCCCGTGGTCAACGGCAA GGAAACGCGCTGGCGCTACCGCGAAGGCAGCGATCCTTATGTCAAGGCCGGTACCGGCTTCCAGTTCTACGGCAATCCCG ACGGCAAGGCCGTGATCTTCGCGCTGCCGTATGAGCCGCCACCGGAATCGCCGGACAAGGAATATCCGTTCTGGCTCGCC ACCGGGCGCGTGCTGGAACACTGGCATTCCGGCTCGATGACGCGGCGTGTGCCCGAACTCTACCGGGCCTTCCCCAACGC GGTGGTGTTCATGCATCCCGAAGACGCCAAGGCCATGGGGCTGCGGCGCGGCGTCGAGGTGGAAGTGGTGTCGCGGCGCG GGCGCATGCGTTCGCGCGTGGAGACACGCGGCCGCGACGCGCCGCCGCGTGGCCTGGTCTTCGTGCCGTGGTTCGATGCA AGCCAGCTCATCAACAAGGTCACGCTCGACGCGACCTGTCCGATTTCGCTGCAGACCGACTACAAGAAGTGCGCGGTGAA GATCGTCAAGGTGTAG
Upstream 100 bases:
>100_bases GCGCAGCTGACCTGCCTTCACCAGCTCGAAGGCGTGCTGTCGGCCACCCTGGTCTATCAGCACAACGAAGACGCCGAAGC CATGAACGAGGAGATCGACG
Downstream 100 bases:
>100_bases CCGCCCACGCCAATCCACAAAAGGAGACGCCATGAAGCCGAGCCGATCCTGGGCACCGCTGCTGGCCTTGTGTACCTGGC TGCTGGCGGCATTTGCATTT
Product: nitrate reductase catalytic subunit
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 831; Mature: 831
Protein sequence:
>831_residues MKVSRRDFIKQTAIAATASVAGIPLGTEAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVAVRDNKVVATQGDPQCEVNKG LNCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPVTWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYA ASKLYKAGFRSNNIDPNARHCMASAVQGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRITDRRLSHPKTR VAVLSTFTHRSFDLADIPIIFTPQTDLAMLNYIANYIIQNNKVNKDFVNKHTVFKEGVTEIGYGLRPDHPLQKAAKNAAN PGDSKPITFDDFAKFVSKYDADYVSKLSGVPKDKLRQLAELYADPNVKVMSLWTMGFNQHTRGSWVNNMVYNVHLLTGKI ATPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIVEKPGYHAVLQNRMLRDGKLNAYW VQVNNNMQAAANIMEEALPGYRNPANFIVVSDAYPTVTALSADLILPSAMWVEKEGAYGNAERRTQFWHQLVDAPGDARS DLWQLMEFSKRFKVEEVWPAELVAKKPEYKGKTLFDVLYRNGQVDKFPIKEVSTEYHNAEAQAFGFYVQKGLFEEYASFG RGHGHDLAPFDRYHEERGLRWPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPPESPDKEYPFWLA TGRVLEHWHSGSMTRRVPELYRAFPNAVVFMHPEDAKAMGLRRGVEVEVVSRRGRMRSRVETRGRDAPPRGLVFVPWFDA SQLINKVTLDATCPISLQTDYKKCAVKIVKV
Sequences:
>Translated_831_residues MKVSRRDFIKQTAIAATASVAGIPLGTEAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVAVRDNKVVATQGDPQCEVNKG LNCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPVTWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYA ASKLYKAGFRSNNIDPNARHCMASAVQGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRITDRRLSHPKTR VAVLSTFTHRSFDLADIPIIFTPQTDLAMLNYIANYIIQNNKVNKDFVNKHTVFKEGVTEIGYGLRPDHPLQKAAKNAAN PGDSKPITFDDFAKFVSKYDADYVSKLSGVPKDKLRQLAELYADPNVKVMSLWTMGFNQHTRGSWVNNMVYNVHLLTGKI ATPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIVEKPGYHAVLQNRMLRDGKLNAYW VQVNNNMQAAANIMEEALPGYRNPANFIVVSDAYPTVTALSADLILPSAMWVEKEGAYGNAERRTQFWHQLVDAPGDARS DLWQLMEFSKRFKVEEVWPAELVAKKPEYKGKTLFDVLYRNGQVDKFPIKEVSTEYHNAEAQAFGFYVQKGLFEEYASFG RGHGHDLAPFDRYHEERGLRWPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPPESPDKEYPFWLA TGRVLEHWHSGSMTRRVPELYRAFPNAVVFMHPEDAKAMGLRRGVEVEVVSRRGRMRSRVETRGRDAPPRGLVFVPWFDA SQLINKVTLDATCPISLQTDYKKCAVKIVKV >Mature_831_residues MKVSRRDFIKQTAIAATASVAGIPLGTEAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVAVRDNKVVATQGDPQCEVNKG LNCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPVTWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYA ASKLYKAGFRSNNIDPNARHCMASAVQGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRITDRRLSHPKTR VAVLSTFTHRSFDLADIPIIFTPQTDLAMLNYIANYIIQNNKVNKDFVNKHTVFKEGVTEIGYGLRPDHPLQKAAKNAAN PGDSKPITFDDFAKFVSKYDADYVSKLSGVPKDKLRQLAELYADPNVKVMSLWTMGFNQHTRGSWVNNMVYNVHLLTGKI ATPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIVEKPGYHAVLQNRMLRDGKLNAYW VQVNNNMQAAANIMEEALPGYRNPANFIVVSDAYPTVTALSADLILPSAMWVEKEGAYGNAERRTQFWHQLVDAPGDARS DLWQLMEFSKRFKVEEVWPAELVAKKPEYKGKTLFDVLYRNGQVDKFPIKEVSTEYHNAEAQAFGFYVQKGLFEEYASFG RGHGHDLAPFDRYHEERGLRWPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPPESPDKEYPFWLA TGRVLEHWHSGSMTRRVPELYRAFPNAVVFMHPEDAKAMGLRRGVEVEVVSRRGRMRSRVETRGRDAPPRGLVFVPWFDA SQLINKVTLDATCPISLQTDYKKCAVKIVKV
Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla
COG id: COG0243
COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]
Homologues:
Organism=Escherichia coli, GI1788534, Length=831, Percent_Identity=70.2767749699158, Blast_Score=1250, Evalue=0.0, Organism=Escherichia coli, GI3868721, Length=562, Percent_Identity=25.4448398576512, Blast_Score=175, Evalue=9e-45, Organism=Escherichia coli, GI3868720, Length=661, Percent_Identity=22.5416036308623, Blast_Score=119, Evalue=1e-27, Organism=Escherichia coli, GI3868719, Length=661, Percent_Identity=21.78517397882, Blast_Score=110, Evalue=3e-25, Organism=Escherichia coli, GI87081797, Length=850, Percent_Identity=21.4117647058824, Blast_Score=87, Evalue=4e-18, Organism=Escherichia coli, GI1787870, Length=627, Percent_Identity=22.9665071770335, Blast_Score=87, Evalue=5e-18, Organism=Escherichia coli, GI171474008, Length=826, Percent_Identity=22.0338983050847, Blast_Score=79, Evalue=1e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR009010 - InterPro: IPR006657 - InterPro: IPR006656 - InterPro: IPR006963 - InterPro: IPR006655 - InterPro: IPR010051 - InterPro: IPR006311 [H]
Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]
EC number: =1.7.99.4 [H]
Molecular weight: Translated: 93763; Mature: 93763
Theoretical pI: Translated: 9.39; Mature: 9.39
Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 4.2 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 4.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKVSRRDFIKQTAIAATASVAGIPLGTEAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVA CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHEEECCCCCHHHCCCCCCEEEE VRDNKVVATQGDPQCEVNKGLNCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPV EECCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC TWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYAASKLYKAGFRSNNIDPNARH CHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH CMASAVQGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRITDRRLSHPKTR HHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCCHHHHCHHHHEEEHHHHHCCCHHH VAVLSTFTHRSFDLADIPIIFTPQTDLAMLNYIANYIIQNNKVNKDFVNKHTVFKEGVTE HHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH IGYGLRPDHPLQKAAKNAANPGDSKPITFDDFAKFVSKYDADYVSKLSGVPKDKLRQLAE HCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH LYADPNVKVMSLWTMGFNQHTRGSWVNNMVYNVHLLTGKIATPGNSPFSLTGQPSACGTA HHCCCCCEEEEEEEECCCCCCCCCHHHCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCH REVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIVEKPGYHAVLQNRMLRDGKLNAYW HHHCCHHHCCCCCEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHCCCCCEEEE VQVNNNMQAAANIMEEALPGYRNPANFIVVSDAYPTVTALSADLILPSAMWVEKEGAYGN EEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHEEECCCCCCC AERRTQFWHQLVDAPGDARSDLWQLMEFSKRFKVEEVWPAELVAKKPEYKGKTLFDVLYR HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHC NGQVDKFPIKEVSTEYHNAEAQAFGFYVQKGLFEEYASFGRGHGHDLAPFDRYHEERGLR CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCC WPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPPESPDKEYPFWLA CCCCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEE TGRVLEHWHSGSMTRRVPELYRAFPNAVVFMHPEDAKAMGLRRGVEVEVVSRRGRMRSRV HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCEEEEHHHHHHHHHHH ETRGRDAPPRGLVFVPWFDASQLINKVTLDATCPISLQTDYKKCAVKIVKV HHCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCEEECCCHHHHHEEEECC >Mature Secondary Structure MKVSRRDFIKQTAIAATASVAGIPLGTEAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVA CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHEEECCCCCHHHCCCCCCEEEE VRDNKVVATQGDPQCEVNKGLNCVKGYFLSKIMYGQDRLTKPLLRMKNGKYDKNGEFAPV EECCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC TWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYAASKLYKAGFRSNNIDPNARH CHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH CMASAVQGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRITDRRLSHPKTR HHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCCHHHHCHHHHEEEHHHHHCCCHHH VAVLSTFTHRSFDLADIPIIFTPQTDLAMLNYIANYIIQNNKVNKDFVNKHTVFKEGVTE HHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH IGYGLRPDHPLQKAAKNAANPGDSKPITFDDFAKFVSKYDADYVSKLSGVPKDKLRQLAE HCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHH LYADPNVKVMSLWTMGFNQHTRGSWVNNMVYNVHLLTGKIATPGNSPFSLTGQPSACGTA HHCCCCCEEEEEEEECCCCCCCCCHHHCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCH REVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIVEKPGYHAVLQNRMLRDGKLNAYW HHHCCHHHCCCCCEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHCCCCCEEEE VQVNNNMQAAANIMEEALPGYRNPANFIVVSDAYPTVTALSADLILPSAMWVEKEGAYGN EEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHEEECCCCCCC AERRTQFWHQLVDAPGDARSDLWQLMEFSKRFKVEEVWPAELVAKKPEYKGKTLFDVLYR HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHC NGQVDKFPIKEVSTEYHNAEAQAFGFYVQKGLFEEYASFGRGHGHDLAPFDRYHEERGLR CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCC WPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPPESPDKEYPFWLA CCCCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEE TGRVLEHWHSGSMTRRVPELYRAFPNAVVFMHPEDAKAMGLRRGVEVEVVSRRGRMRSRV HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCEEEEHHHHHHHHHHH ETRGRDAPPRGLVFVPWFDASQLINKVTLDATCPISLQTDYKKCAVKIVKV HHCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCEEECCCHHHHHEEEECC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8376334; 12948488 [H]