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The map label for this gene is alkJ [H]

Identifier: 73538574

GI number: 73538574

Start: 1447836

End: 1449476

Strand: Reverse

Name: alkJ [H]

Synonym: Reut_B4749

Alternate gene names: 73538574

Gene position: 1449476-1447836 (Counterclockwise)

Preceding gene: 73538575

Following gene: 73538569

Centisome position: 53.17

GC content: 64.17

Gene sequence:

>1641_bases
ATGGAAACGTTTGACTATCTGGTGGTGGGTGCCGGCTCGGCAGGCTGCGCCATTGCATCAAGGCTGGCCGAAGACCCCGC
CGTGACGGTGGCCCTGATCGAGGCCGGGCCGACTGATCACCATATGAGCATCTGGATGCCGATCGGCATGGCATCTACCG
TGCGCAATGCCGGCCCACGCAACTACGGCTACTACACCGTGCCGCAGCCGGGCTTCAACGGGCGGCAGGGTTACCAGCCG
CGCGGCAAGGGTCTGGGCGGCAGCTCGTCGATCAACGGCATGGTCTATATCCGCGGTCACCGCAACGACTACGACGACTG
GGCGCGCCTGGGCTGCACCGGCTGGGGATATGAAGACGTGCTGCCGTACTTCCGGCGCAGCGAACATCACGAGGACTACA
GCGGCCGAGACGATAACCGATGGCACGGTGGCACGGGCCCGCTGCGCGTAAGCAACCTGCGCTCGCCCAGCCCGTTTTCC
CGGCGCTTTATCGACGCGGCAATCCAGGCTGGCTATCGGCCCAATAGCGATTTCAACGGTGCGGATCAGGAGGGAGCGGG
CTTCTACCACGTCACCCAGCATCGTGGCGAGCGCTGGAATTCGGCGCGCGCCTACCTGCATCAGGGCAACGCGGGCGACA
AGACTCTGAGCGGAGGCCGCAGCAACCTTTCGGTGCTGGTCGAGACGCAGGTGCTGCGTATCAACTTCGACGGCAGGCGG
GCCACGGGACTGACCGTTGTGCGCGGCGGGGTGGAGCAGAAGCTGGCGGCACGGCGCGAAGTCATTGTCAGCGCCGGGGC
CTTCAACTCGCCGCAGTTGCTGCTGGCGTCCGGCGTAGGCCCGGCGCGCGAGCTTTTTGACATGGGGATTCATGTTGTAG
CCGACCTGCCTGGCGTAGGTAGGAACCTGCAGGACCATCCGGATGTCATCCTGAACAAGCAGGTGCATTCGCTGGACCTG
TTTGGCCATTCCTTTGGCGGCTTTGTGAAGCTGTCGATGGAGTTGCTGCGCTATCGCCGCAAACGCACCGGCATGCCTAG
CAGCAATTTCGCGGAGGCCGGCGCTTTCATCAAGAGTCGGCAGGGACTTGCGGCCCCTGATATTCAGCTGCATTTCATCA
CGGCACTGCTCAATCACGGCAGCGACAGGAAGAAGCTGGGTCATGGCTATTCCTGCCATGCCTGCGTGCTGCGGCCGAAG
AGTGTTGGAGAAGTACGATTGCGTTCCCCGGACATGCGCGAAGCACCGGTCATCGATCCGCGCTTCCTCAGCCATGAGGA
AGATATGACCGTCATGGTGGCAGGCGTGCGCGCAATCCGCCGCATCTTCGCGCAGCAGGCGCTGGCCAGCGCCGGCGGCC
GCGAGCTGTTCACCGACGAATTCGGGCCGGGCGACGGCGACCAGCAAGCGATCGAGGCTTTCGTGCGCGAGCGGACCGAC
AGCGTCTACCACCCGGTCGGCACCTGCAAGATGGGTGTGGATGACATGGCCGTGGTCGATCCCGCCTTGCGTGTGCGTGG
TGTGCAGGGCTTGCGCGTGGTCGATGCATCGATCATGCCGACGCTGGTGGCAGGCAACACCAATGCGCCGGCGATCATGA
TCGCCGAGAAGGCGGCAGACCTGATCCGCGCCGGCGACTAA

Upstream 100 bases:

>100_bases
GGCAACGGCCGCGAGTTCGGCGCGTACGGCCTGGCCGAGTTCCTCGAATACAAGTCGGTCGCAGGCTGCACCGTGGATGA
ATGAAGGAGTGACCGATTAC

Downstream 100 bases:

>100_bases
CGGGACGGCACTGCAATCGTCTTGCCGGCCCGCAGTGCCGTGAGGTGCTGCGGGCCGGGGCGTCGGGCCGGCTTCAGAGA
TAGGTAGCCACAACCACGTA

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
METFDYLVVGAGSAGCAIASRLAEDPAVTVALIEAGPTDHHMSIWMPIGMASTVRNAGPRNYGYYTVPQPGFNGRQGYQP
RGKGLGGSSSINGMVYIRGHRNDYDDWARLGCTGWGYEDVLPYFRRSEHHEDYSGRDDNRWHGGTGPLRVSNLRSPSPFS
RRFIDAAIQAGYRPNSDFNGADQEGAGFYHVTQHRGERWNSARAYLHQGNAGDKTLSGGRSNLSVLVETQVLRINFDGRR
ATGLTVVRGGVEQKLAARREVIVSAGAFNSPQLLLASGVGPARELFDMGIHVVADLPGVGRNLQDHPDVILNKQVHSLDL
FGHSFGGFVKLSMELLRYRRKRTGMPSSNFAEAGAFIKSRQGLAAPDIQLHFITALLNHGSDRKKLGHGYSCHACVLRPK
SVGEVRLRSPDMREAPVIDPRFLSHEEDMTVMVAGVRAIRRIFAQQALASAGGRELFTDEFGPGDGDQQAIEAFVRERTD
SVYHPVGTCKMGVDDMAVVDPALRVRGVQGLRVVDASIMPTLVAGNTNAPAIMIAEKAADLIRAGD

Sequences:

>Translated_546_residues
METFDYLVVGAGSAGCAIASRLAEDPAVTVALIEAGPTDHHMSIWMPIGMASTVRNAGPRNYGYYTVPQPGFNGRQGYQP
RGKGLGGSSSINGMVYIRGHRNDYDDWARLGCTGWGYEDVLPYFRRSEHHEDYSGRDDNRWHGGTGPLRVSNLRSPSPFS
RRFIDAAIQAGYRPNSDFNGADQEGAGFYHVTQHRGERWNSARAYLHQGNAGDKTLSGGRSNLSVLVETQVLRINFDGRR
ATGLTVVRGGVEQKLAARREVIVSAGAFNSPQLLLASGVGPARELFDMGIHVVADLPGVGRNLQDHPDVILNKQVHSLDL
FGHSFGGFVKLSMELLRYRRKRTGMPSSNFAEAGAFIKSRQGLAAPDIQLHFITALLNHGSDRKKLGHGYSCHACVLRPK
SVGEVRLRSPDMREAPVIDPRFLSHEEDMTVMVAGVRAIRRIFAQQALASAGGRELFTDEFGPGDGDQQAIEAFVRERTD
SVYHPVGTCKMGVDDMAVVDPALRVRGVQGLRVVDASIMPTLVAGNTNAPAIMIAEKAADLIRAGD
>Mature_546_residues
METFDYLVVGAGSAGCAIASRLAEDPAVTVALIEAGPTDHHMSIWMPIGMASTVRNAGPRNYGYYTVPQPGFNGRQGYQP
RGKGLGGSSSINGMVYIRGHRNDYDDWARLGCTGWGYEDVLPYFRRSEHHEDYSGRDDNRWHGGTGPLRVSNLRSPSPFS
RRFIDAAIQAGYRPNSDFNGADQEGAGFYHVTQHRGERWNSARAYLHQGNAGDKTLSGGRSNLSVLVETQVLRINFDGRR
ATGLTVVRGGVEQKLAARREVIVSAGAFNSPQLLLASGVGPARELFDMGIHVVADLPGVGRNLQDHPDVILNKQVHSLDL
FGHSFGGFVKLSMELLRYRRKRTGMPSSNFAEAGAFIKSRQGLAAPDIQLHFITALLNHGSDRKKLGHGYSCHACVLRPK
SVGEVRLRSPDMREAPVIDPRFLSHEEDMTVMVAGVRAIRRIFAQQALASAGGRELFTDEFGPGDGDQQAIEAFVRERTD
SVYHPVGTCKMGVDDMAVVDPALRVRGVQGLRVVDASIMPTLVAGNTNAPAIMIAEKAADLIRAGD

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=554, Percent_Identity=44.5848375451264, Blast_Score=422, Evalue=1e-118,
Organism=Escherichia coli, GI1786503, Length=552, Percent_Identity=40.7608695652174, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17532301, Length=556, Percent_Identity=37.410071942446, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24642039, Length=579, Percent_Identity=36.6148531951641, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI45551458, Length=576, Percent_Identity=37.1527777777778, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI45549471, Length=576, Percent_Identity=37.3263888888889, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24642048, Length=595, Percent_Identity=34.6218487394958, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24642055, Length=592, Percent_Identity=35.304054054054, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24642042, Length=587, Percent_Identity=35.7751277683135, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24642035, Length=595, Percent_Identity=35.4621848739496, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI18859995, Length=574, Percent_Identity=36.2369337979094, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24642059, Length=595, Percent_Identity=33.4453781512605, Blast_Score=288, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24642037, Length=584, Percent_Identity=33.5616438356164, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI17137792, Length=566, Percent_Identity=35.3356890459364, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24650267, Length=584, Percent_Identity=34.4178082191781, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI18859993, Length=587, Percent_Identity=33.7308347529813, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24642051, Length=586, Percent_Identity=31.5699658703072, Blast_Score=243, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24645930, Length=597, Percent_Identity=28.9782244556114, Blast_Score=172, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24642057, Length=601, Percent_Identity=31.4475873544093, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59418; Mature: 59418

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFDYLVVGAGSAGCAIASRLAEDPAVTVALIEAGPTDHHMSIWMPIGMASTVRNAGPR
CCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHCCCCC
NYGYYTVPQPGFNGRQGYQPRGKGLGGSSSINGMVYIRGHRNDYDDWARLGCTGWGYEDV
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCHHHH
LPYFRRSEHHEDYSGRDDNRWHGGTGPLRVSNLRSPSPFSRRFIDAAIQAGYRPNSDFNG
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
ADQEGAGFYHVTQHRGERWNSARAYLHQGNAGDKTLSGGRSNLSVLVETQVLRINFDGRR
CCCCCCCEEEEHHHCCCCCCHHHHHEECCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCC
ATGLTVVRGGVEQKLAARREVIVSAGAFNSPQLLLASGVGPARELFDMGIHVVADLPGVG
CCCEEEEECCHHHHHHHHHHHEEECCCCCCCCEEEECCCCHHHHHHHCCEEEEECCCCCC
RNLQDHPDVILNKQVHSLDLFGHSFGGFVKLSMELLRYRRKRTGMPSSNFAEAGAFIKSR
CCCCCCCCEEECCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
QGLAAPDIQLHFITALLNHGSDRKKLGHGYSCHACVLRPKSVGEVRLRSPDMREAPVIDP
CCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCC
RFLSHEEDMTVMVAGVRAIRRIFAQQALASAGGRELFTDEFGPGDGDQQAIEAFVRERTD
HHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHCC
SVYHPVGTCKMGVDDMAVVDPALRVRGVQGLRVVDASIMPTLVAGNTNAPAIMIAEKAAD
CHHCCCCCEECCCCCHHHHCHHHHHCCCCCEEEECCCCCCEEEECCCCCCEEEEEHHHHH
LIRAGD
HHHCCC
>Mature Secondary Structure
METFDYLVVGAGSAGCAIASRLAEDPAVTVALIEAGPTDHHMSIWMPIGMASTVRNAGPR
CCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHCCCCC
NYGYYTVPQPGFNGRQGYQPRGKGLGGSSSINGMVYIRGHRNDYDDWARLGCTGWGYEDV
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCHHHH
LPYFRRSEHHEDYSGRDDNRWHGGTGPLRVSNLRSPSPFSRRFIDAAIQAGYRPNSDFNG
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
ADQEGAGFYHVTQHRGERWNSARAYLHQGNAGDKTLSGGRSNLSVLVETQVLRINFDGRR
CCCCCCCEEEEHHHCCCCCCHHHHHEECCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCC
ATGLTVVRGGVEQKLAARREVIVSAGAFNSPQLLLASGVGPARELFDMGIHVVADLPGVG
CCCEEEEECCHHHHHHHHHHHEEECCCCCCCCEEEECCCCHHHHHHHCCEEEEECCCCCC
RNLQDHPDVILNKQVHSLDLFGHSFGGFVKLSMELLRYRRKRTGMPSSNFAEAGAFIKSR
CCCCCCCCEEECCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
QGLAAPDIQLHFITALLNHGSDRKKLGHGYSCHACVLRPKSVGEVRLRSPDMREAPVIDP
CCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCC
RFLSHEEDMTVMVAGVRAIRRIFAQQALASAGGRELFTDEFGPGDGDQQAIEAFVRERTD
HHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHCC
SVYHPVGTCKMGVDDMAVVDPALRVRGVQGLRVVDASIMPTLVAGNTNAPAIMIAEKAAD
CHHCCCCCEECCCCCHHHHCHHHHHCCCCCEEEECCCCCCEEEECCCCCCEEEEEHHHHH
LIRAGD
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]