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The map label for this gene is vanB [H]

Identifier: 73538171

GI number: 73538171

Start: 1001215

End: 1002156

Strand: Direct

Name: vanB [H]

Synonym: Reut_B4341

Alternate gene names: 73538171

Gene position: 1001215-1002156 (Clockwise)

Preceding gene: 73538170

Following gene: 73538172

Centisome position: 36.73

GC content: 65.07

Gene sequence:

>942_bases
ATGGATCTCGTCGTGACAAGCCTGCGCCTGGAGGCGGAAGGCGTGCTTGGCGTGGAACTCAGGCATCGTTCAGGCGATGC
CCTGCCGCCATTCGAACCGGGCGCGCATGTGGATGTCAGCTTTCCCAACGGTTTGACGCGGCAATACTCGATCGCCAGCA
CGGCCTCAGACACCACAAGCTACTGGCTTGGCATCGGGCTCGCACCGGCCTCACGGGGCGGCTCGCGCTTTGCGCACCAC
GAACTGCGCCTGGGCGACACCCTGCCGGTCGGCACGCCGCGGTCGCTGTTCGGGCTGCACGAGCCAGCCGCCGGCCATCT
GTTCGTCGCCGGAGGCATCGGGATCACGCCGATCCTGAGCATGATCCGGCGCTGCGTCGAACGCGATCTTCCGTGGTGGC
TGCTGTACTGCGTTCGCTCGCGCCGCCATGCCGCCTATCTGGAGCAGCTCGCACCGTTCGCGAACCGCGTCACGCTGCAT
GCCGACGACGAGCATGGCGGCCATCCCGACGTCCATACGGCATTGCGGCAAATGCCCGCAGGCTGGCACGTCTACACCTG
CGGACCGGGAGTCATGATGGATGCGGTATGCGATCACGCCAGCGCATCGGGAATCGGTACCCATGCGGTGCATCTCGAAC
GCTTCAGTGCCGGCACACAGGCACCTGCGGAAAGCGGTGCGTTCCAGGTACGCCTGCTCAGGCACGGCGGACAATTCCCG
GTGCCGGCAGGGACCAGCATCCTCGAAGTGCTGGAAGATAACGGTGTCTGCCTGCCATCGTCATGCCGGAAAGGCCTGTG
CCGCAGTTGCGAAGTCCCGCTGGTTGCCGGCACGGCGGATCACCACGACTATGTGCTCAGCGATGAAGAACGCGCGGCCA
ACAAGTCCATCCTGATCTGCGTTTCGCGCGCGAAATGTGCGGAGCTGGTGCTGGACGTGTAG

Upstream 100 bases:

>100_bases
TCTCGATCGATGCCGCGCTGTCGCAGTTCCGCTGGGTTGTCGAGCGCCGTGTCGCAGACGAAGCCGCACAGGCGCAGCAG
CGTCAGGCGAAGGAGGCGTG

Downstream 100 bases:

>100_bases
TTCACTGCGAGAGCAGGCCACCATCTTGCCTTACCATGGCGGCCTGCTTCCGAGACTCCGCCATGTCCCGTTTTCCGCAC
CGCGCCCTGCTCTGCCTGTC

Product: ferredoxin:oxidoreductase FAD/NAD(P)-binding:oxidoreductase FAD-binding region

Products: NA

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MDLVVTSLRLEAEGVLGVELRHRSGDALPPFEPGAHVDVSFPNGLTRQYSIASTASDTTSYWLGIGLAPASRGGSRFAHH
ELRLGDTLPVGTPRSLFGLHEPAAGHLFVAGGIGITPILSMIRRCVERDLPWWLLYCVRSRRHAAYLEQLAPFANRVTLH
ADDEHGGHPDVHTALRQMPAGWHVYTCGPGVMMDAVCDHASASGIGTHAVHLERFSAGTQAPAESGAFQVRLLRHGGQFP
VPAGTSILEVLEDNGVCLPSSCRKGLCRSCEVPLVAGTADHHDYVLSDEERAANKSILICVSRAKCAELVLDV

Sequences:

>Translated_313_residues
MDLVVTSLRLEAEGVLGVELRHRSGDALPPFEPGAHVDVSFPNGLTRQYSIASTASDTTSYWLGIGLAPASRGGSRFAHH
ELRLGDTLPVGTPRSLFGLHEPAAGHLFVAGGIGITPILSMIRRCVERDLPWWLLYCVRSRRHAAYLEQLAPFANRVTLH
ADDEHGGHPDVHTALRQMPAGWHVYTCGPGVMMDAVCDHASASGIGTHAVHLERFSAGTQAPAESGAFQVRLLRHGGQFP
VPAGTSILEVLEDNGVCLPSSCRKGLCRSCEVPLVAGTADHHDYVLSDEERAANKSILICVSRAKCAELVLDV
>Mature_313_residues
MDLVVTSLRLEAEGVLGVELRHRSGDALPPFEPGAHVDVSFPNGLTRQYSIASTASDTTSYWLGIGLAPASRGGSRFAHH
ELRLGDTLPVGTPRSLFGLHEPAAGHLFVAGGIGITPILSMIRRCVERDLPWWLLYCVRSRRHAAYLEQLAPFANRVTLH
ADDEHGGHPDVHTALRQMPAGWHVYTCGPGVMMDAVCDHASASGIGTHAVHLERFSAGTQAPAESGAFQVRLLRHGGQFP
VPAGTSILEVLEDNGVCLPSSCRKGLCRSCEVPLVAGTADHHDYVLSDEERAANKSILICVSRAKCAELVLDV

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=322, Percent_Identity=38.5093167701863, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1787098, Length=284, Percent_Identity=27.112676056338, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787658, Length=322, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 33610; Mature: 33610

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLVVTSLRLEAEGVLGVELRHRSGDALPPFEPGAHVDVSFPNGLTRQYSIASTASDTTS
CCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCE
YWLGIGLAPASRGGSRFAHHELRLGDTLPVGTPRSLFGLHEPAAGHLFVAGGIGITPILS
EEEEEECCCCCCCCCCCHHHEEECCCCCCCCCCHHHHCCCCCCCCCEEEECCCCHHHHHH
MIRRCVERDLPWWLLYCVRSRRHAAYLEQLAPFANRVTLHADDEHGGHPDVHTALRQMPA
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCC
GWHVYTCGPGVMMDAVCDHASASGIGTHAVHLERFSAGTQAPAESGAFQVRLLRHGGQFP
CCEEEECCCCHHHHHHHCCCCCCCCCHHHEEEHHHCCCCCCCCCCCCEEEEEECCCCCCC
VPAGTSILEVLEDNGVCLPSSCRKGLCRSCEVPLVAGTADHHDYVLSDEERAANKSILIC
CCCCHHHHHHHHCCCEECCHHHHHHHHHCCCCCEEECCCCCCCEEECCHHHHCCCEEEEE
VSRAKCAELVLDV
ECCHHHHHHHCCC
>Mature Secondary Structure
MDLVVTSLRLEAEGVLGVELRHRSGDALPPFEPGAHVDVSFPNGLTRQYSIASTASDTTS
CCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCE
YWLGIGLAPASRGGSRFAHHELRLGDTLPVGTPRSLFGLHEPAAGHLFVAGGIGITPILS
EEEEEECCCCCCCCCCCHHHEEECCCCCCCCCCHHHHCCCCCCCCCEEEECCCCHHHHHH
MIRRCVERDLPWWLLYCVRSRRHAAYLEQLAPFANRVTLHADDEHGGHPDVHTALRQMPA
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCC
GWHVYTCGPGVMMDAVCDHASASGIGTHAVHLERFSAGTQAPAESGAFQVRLLRHGGQFP
CCEEEECCCCHHHHHHHCCCCCCCCCHHHEEEHHHCCCCCCCCCCCCEEEEEECCCCCCC
VPAGTSILEVLEDNGVCLPSSCRKGLCRSCEVPLVAGTADHHDYVLSDEERAANKSILIC
CCCCHHHHHHHHCCCEECCHHHHHHHHHCCCCCEEECCCCCCCEEECCHHHHCCCEEEEE
VSRAKCAELVLDV
ECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3170489 [H]