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The map label for this gene is estA [H]

Identifier: 73538127

GI number: 73538127

Start: 956879

End: 957577

Strand: Direct

Name: estA [H]

Synonym: Reut_B4297

Alternate gene names: 73538127

Gene position: 956879-957577 (Clockwise)

Preceding gene: 73538126

Following gene: 73538128

Centisome position: 35.1

GC content: 64.52

Gene sequence:

>699_bases
ATGAACGAACTGCTGCCCGCGATCGAAATCGAGACCGCGCCGAATCCCGCTTTCAGCGTCATCTGGATGCACGGGCTCGG
TGCCGACGGCAACGACTTCGTCCCGGTAGTGCCGGAACTCGGGCTCGACGCCGCGCCTGGGGTGCGCTTTATCTTCCCGC
ATGCGCCCGCCATTCCCGTTACGTGCAACGGCGGCTACGTCATGCCAGCGTGGTATGACATCTACTTGCTCGGAGAAACC
AGCCGGCACGCGGACGAACAGGGCATCCGTCTTTCGCGCGAACGCATCCGCGCGCTGATTGCGCGCGAAAATGCGCGCGG
CATCGCGACCAGCCACATTGTCCTTGCGGGCTTCTCGCAAGGTGGCGCAATCGCCTATACCACTGCCCTCACCCATCCCG
ATGCGCTTGCGGGCATCATCGCGCTGTCCACCTACATCCCCGCGCCGGATCTTTTGACCGCGGAAGCCACGACTGCGAAC
GCCGCCATCCCGGTCTTCGCCGCGCATGGCACGCAGGACGACGTCGTATCGCTGCGCCTCGGCGAGCAGGCCCGCGATTT
CCTGCAGGCACGCAGCATTCCAGTCGCATGGCACACCTATGCCATGCCGCATTCGGTCTGCATGGACGAAATCGCGGCGA
TCGGCGCGTGGCTCAATGCCACCTTTGCGCGGATCACGGAGCAGACGGCGCAACGCTAA

Upstream 100 bases:

>100_bases
GTGTTGCCGGCCTGCGCGCATAGACCTGCGCCGACCTGTGCGATACTCGCGCTGCCCGCCGCGCAAGACGGCGTCTGCCT
GATCAGCCTGGAGTGCCCCC

Downstream 100 bases:

>100_bases
AACACGGCACGCTCCACTGTCATCACCACGTATATGAAAGCCGCCCCGCTTCTGAAGATCGATTTCAATACCCGCAAGGC
CCTGATGTTCGCGCTGACCG

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNELLPAIEIETAPNPAFSVIWMHGLGADGNDFVPVVPELGLDAAPGVRFIFPHAPAIPVTCNGGYVMPAWYDIYLLGET
SRHADEQGIRLSRERIRALIARENARGIATSHIVLAGFSQGGAIAYTTALTHPDALAGIIALSTYIPAPDLLTAEATTAN
AAIPVFAAHGTQDDVVSLRLGEQARDFLQARSIPVAWHTYAMPHSVCMDEIAAIGAWLNATFARITEQTAQR

Sequences:

>Translated_232_residues
MNELLPAIEIETAPNPAFSVIWMHGLGADGNDFVPVVPELGLDAAPGVRFIFPHAPAIPVTCNGGYVMPAWYDIYLLGET
SRHADEQGIRLSRERIRALIARENARGIATSHIVLAGFSQGGAIAYTTALTHPDALAGIIALSTYIPAPDLLTAEATTAN
AAIPVFAAHGTQDDVVSLRLGEQARDFLQARSIPVAWHTYAMPHSVCMDEIAAIGAWLNATFARITEQTAQR
>Mature_232_residues
MNELLPAIEIETAPNPAFSVIWMHGLGADGNDFVPVVPELGLDAAPGVRFIFPHAPAIPVTCNGGYVMPAWYDIYLLGET
SRHADEQGIRLSRERIRALIARENARGIATSHIVLAGFSQGGAIAYTTALTHPDALAGIIALSTYIPAPDLLTAEATTAN
AAIPVFAAHGTQDDVVSLRLGEQARDFLQARSIPVAWHTYAMPHSVCMDEIAAIGAWLNATFARITEQTAQR

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI9966764, Length=204, Percent_Identity=39.2156862745098, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI5453722, Length=229, Percent_Identity=35.3711790393013, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI20270341, Length=205, Percent_Identity=24.390243902439, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508057, Length=208, Percent_Identity=36.5384615384615, Blast_Score=130, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6323147, Length=230, Percent_Identity=32.1739130434783, Blast_Score=119, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45553055, Length=206, Percent_Identity=34.4660194174757, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45553057, Length=206, Percent_Identity=34.4660194174757, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21357257, Length=206, Percent_Identity=34.4660194174757, Blast_Score=121, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24820; Mature: 24820

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELLPAIEIETAPNPAFSVIWMHGLGADGNDFVPVVPELGLDAAPGVRFIFPHAPAIPV
CCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCCEE
TCNGGYVMPAWYDIYLLGETSRHADEQGIRLSRERIRALIARENARGIATSHIVLAGFSQ
EECCCEECCCEEEEEEEECCCCCCCHHCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCC
GGAIAYTTALTHPDALAGIIALSTYIPAPDLLTAEATTANAAIPVFAAHGTQDDVVSLRL
CCEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCCEEEEEE
GEQARDFLQARSIPVAWHTYAMPHSVCMDEIAAIGAWLNATFARITEQTAQR
CHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNELLPAIEIETAPNPAFSVIWMHGLGADGNDFVPVVPELGLDAAPGVRFIFPHAPAIPV
CCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCCEE
TCNGGYVMPAWYDIYLLGETSRHADEQGIRLSRERIRALIARENARGIATSHIVLAGFSQ
EECCCEECCCEEEEEEEECCCCCCCHHCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCC
GGAIAYTTALTHPDALAGIIALSTYIPAPDLLTAEATTANAAIPVFAAHGTQDDVVSLRL
CCEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCCEEEEEE
GEQARDFLQARSIPVAWHTYAMPHSVCMDEIAAIGAWLNATFARITEQTAQR
CHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA