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The map label for this gene is cho [H]

Identifier: 73537451

GI number: 73537451

Start: 178243

End: 179835

Strand: Reverse

Name: cho [H]

Synonym: Reut_B3616

Alternate gene names: 73537451

Gene position: 179835-178243 (Counterclockwise)

Preceding gene: 73537454

Following gene: 73537450

Centisome position: 6.6

GC content: 64.34

Gene sequence:

>1593_bases
ATGCAGGACATTTTCCCTCCCCCCTCTGATCGCCTCTCAGGAAAGGCCCGCTTCGCCGACGCCCATGCACTGGCGGCTGC
GCTGTCGCGCCCCATAGTGTTCGTGGATCTGGAAACCACCGGCGCCGATGCCCAGCACGACCGGATCACCGAGATCGGCG
TTGTCGAAGTCGGCCCCGAAGGCATCCAGGAATGGGATACGTTGCTTGATCCCGGCGTAAGCATCCCACCATTCATCCAG
CGGCTGACGGGCATCACCGACGAGACCGTACAAGGCCAGCCCACTTTTGCCTCGATCGCTGAAGCCCTTGCCGAGCGCCT
GCAGGGACGCCTTTTCGTCGCCCACAACGCGCGCTTTGACTATGGATTCCTGAAGAACGAGTTCCGCCGCGCCGGATTGA
CTTTCCGCGCCGACGTACTGTGCACGGTCAGGCTGTCACGCTCGCTGTTCCCTTCAGCGGCACGGCATGGCCTTGATGCG
ATCATCGCGCGGTTCGACTTGCAACCCCGGGCGCGCCATCGGGCACTGGCCGATGCCGAGTTGCTGTGGCAGTTCTGGCA
GAAGATCCATGCGCTCTATTCCGTGGAACTGATCGATGCGGCAACGGGAGCCCTGGTCAAGCGTGCCAGCCTGCCTGCAG
GCCTCGAAGAAACCGCGCTCGACGATGTGCCCGATACCGCCGGTGTCTATCTGTTCTACGGCGATAACGACGCCCCGCTC
TATATAGGTAAGAGCGTGCATCTGCGGCAACGGATCGGCGCACATTTCTCGGGTGACTACCGGCTAGGCAAGGACATGCG
CCTCGCCCGCCTGGTGCGACGCGTGGAATGGCGTGAGACCGGAGGAGAAATCGGTGCCCTGTTGCTCGAGGCAAGTCTGG
TAAAAACCATGCAGCCGCTCCACAACCAGATGCTGCGCGGAAGCGCTCGGCTGTACACCTGGGAACTGCCGCCCGAATTG
CCTGTGCCGCGCCTGCGCTCGGACGCCGATACCGATTTCAGCCGACGGGGCAATCTGTATGGCGCGTTCGCGAGCCGTGG
CGCAGCGCAAGCCTGGCTGCGCCGGCTGGCCGATGAGCATCGGCTTTGCCAGGCGACCCTAACCCTGGAGAAGACCGCGC
GCGCGGGCAATCCCTGCTTCGCGCGGCAGCTCCACCGCTGCGAGGGCGCCTGTGTGGGCGAAGAGGCGTCGCCAAGCCAC
CGGGCGAGAATCGCGACAGCCATGGCTGAGGCGCGGCTCAAACGCTGGCCATTTGACGGTCCGGCCGCCTGGCCGGAAGT
CTCGGGCACGCAAACCTGGTGGCACGTTGCAGAGGACTGGTGCTATCTGGGGTCGGTGCAAACACTTGAAGACGTCACCA
CCCTGCTTGCCGCGCCGGCACGCTTCGATCTGGATACTTACCAGATCCTTGCTTCGCGGCTGCAGCAGTGGATGCCCAAT
GCGGTGCCGCTGACATCCACACGCAGCTTCGAGCTTGCCTTGTGCGAAGCGCCTGCGCCTGATGACCGAAGCCCGCCGAA
CCTGCCACAGGGGCCCGGACGAAGCAAGGCGCCGGTGCGGCGCCAGCCGTCGCCTGGCTTGTTTGATGACTGA

Upstream 100 bases:

>100_bases
GTGTTGCGAGGGAGCGAATATTACGGCAGCAAGTCAGGCTTGGCAAGCGCTTTTTGAGAACTGTCTCACATCACCCTGCC
AGCCCACTTAGAATCTGCCC

Downstream 100 bases:

>100_bases
TGGGTCTAAGTTCCCGGACTTGAGTGCGCCACCACGCATAGCCGGCTTGCACCGGCCGAAGCGGCTCCGCTAGCCTTCAC
TGAGGGGCCTGGCGCCCTGA

Product: DNA polymerase III subunit epsilon

Products: diphosphate; DNAn+1

Alternate protein names: Endonuclease cho; UvrC homolog protein [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MQDIFPPPSDRLSGKARFADAHALAAALSRPIVFVDLETTGADAQHDRITEIGVVEVGPEGIQEWDTLLDPGVSIPPFIQ
RLTGITDETVQGQPTFASIAEALAERLQGRLFVAHNARFDYGFLKNEFRRAGLTFRADVLCTVRLSRSLFPSAARHGLDA
IIARFDLQPRARHRALADAELLWQFWQKIHALYSVELIDAATGALVKRASLPAGLEETALDDVPDTAGVYLFYGDNDAPL
YIGKSVHLRQRIGAHFSGDYRLGKDMRLARLVRRVEWRETGGEIGALLLEASLVKTMQPLHNQMLRGSARLYTWELPPEL
PVPRLRSDADTDFSRRGNLYGAFASRGAAQAWLRRLADEHRLCQATLTLEKTARAGNPCFARQLHRCEGACVGEEASPSH
RARIATAMAEARLKRWPFDGPAAWPEVSGTQTWWHVAEDWCYLGSVQTLEDVTTLLAAPARFDLDTYQILASRLQQWMPN
AVPLTSTRSFELALCEAPAPDDRSPPNLPQGPGRSKAPVRRQPSPGLFDD

Sequences:

>Translated_530_residues
MQDIFPPPSDRLSGKARFADAHALAAALSRPIVFVDLETTGADAQHDRITEIGVVEVGPEGIQEWDTLLDPGVSIPPFIQ
RLTGITDETVQGQPTFASIAEALAERLQGRLFVAHNARFDYGFLKNEFRRAGLTFRADVLCTVRLSRSLFPSAARHGLDA
IIARFDLQPRARHRALADAELLWQFWQKIHALYSVELIDAATGALVKRASLPAGLEETALDDVPDTAGVYLFYGDNDAPL
YIGKSVHLRQRIGAHFSGDYRLGKDMRLARLVRRVEWRETGGEIGALLLEASLVKTMQPLHNQMLRGSARLYTWELPPEL
PVPRLRSDADTDFSRRGNLYGAFASRGAAQAWLRRLADEHRLCQATLTLEKTARAGNPCFARQLHRCEGACVGEEASPSH
RARIATAMAEARLKRWPFDGPAAWPEVSGTQTWWHVAEDWCYLGSVQTLEDVTTLLAAPARFDLDTYQILASRLQQWMPN
AVPLTSTRSFELALCEAPAPDDRSPPNLPQGPGRSKAPVRRQPSPGLFDD
>Mature_530_residues
MQDIFPPPSDRLSGKARFADAHALAAALSRPIVFVDLETTGADAQHDRITEIGVVEVGPEGIQEWDTLLDPGVSIPPFIQ
RLTGITDETVQGQPTFASIAEALAERLQGRLFVAHNARFDYGFLKNEFRRAGLTFRADVLCTVRLSRSLFPSAARHGLDA
IIARFDLQPRARHRALADAELLWQFWQKIHALYSVELIDAATGALVKRASLPAGLEETALDDVPDTAGVYLFYGDNDAPL
YIGKSVHLRQRIGAHFSGDYRLGKDMRLARLVRRVEWRETGGEIGALLLEASLVKTMQPLHNQMLRGSARLYTWELPPEL
PVPRLRSDADTDFSRRGNLYGAFASRGAAQAWLRRLADEHRLCQATLTLEKTARAGNPCFARQLHRCEGACVGEEASPSH
RARIATAMAEARLKRWPFDGPAAWPEVSGTQTWWHVAEDWCYLGSVQTLEDVTTLLAAPARFDLDTYQILASRLQQWMPN
AVPLTSTRSFELALCEAPAPDDRSPPNLPQGPGRSKAPVRRQPSPGLFDD

Specific function: Incises the DNA at the 3' side of a lesion during nucleotide excision repair. Incises the DNA farther away from the lesion than uvrC. Not able to incise the 5' site of a lesion. When a lesion remains because uvrC is not able to induce the 3' incision, cho

COG id: COG0322

COG function: function code L; Nuclease subunit of the excinuclease complex

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrC C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788037, Length=260, Percent_Identity=42.3076923076923, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1786409, Length=177, Percent_Identity=32.7683615819209, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000305 [H]

Pfam domain/function: PF01541 GIY-YIG [H]

EC number: 2.7.7.7

Molecular weight: Translated: 58791; Mature: 58791

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS50164 UVRC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIFPPPSDRLSGKARFADAHALAAALSRPIVFVDLETTGADAQHDRITEIGVVEVGPE
CCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEECHH
GIQEWDTLLDPGVSIPPFIQRLTGITDETVQGQPTFASIAEALAERLQGRLFVAHNARFD
HHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
YGFLKNEFRRAGLTFRADVLCTVRLSRSLFPSAARHGLDAIIARFDLQPRARHRALADAE
HHHHHHHHHHCCCCEEEEEEEEEHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLWQFWQKIHALYSVELIDAATGALVKRASLPAGLEETALDDVPDTAGVYLFYGDNDAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEECCCCCCE
YIGKSVHLRQRIGAHFSGDYRLGKDMRLARLVRRVEWRETGGEIGALLLEASLVKTMQPL
EECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
HNQMLRGSARLYTWELPPELPVPRLRSDADTDFSRRGNLYGAFASRGAAQAWLRRLADEH
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCEEEHHHCCCHHHHHHHHHHHHH
RLCQATLTLEKTARAGNPCFARQLHRCEGACVGEEASPSHRARIATAMAEARLKRWPFDG
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PAAWPEVSGTQTWWHVAEDWCYLGSVQTLEDVTTLLAAPARFDLDTYQILASRLQQWMPN
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
AVPLTSTRSFELALCEAPAPDDRSPPNLPQGPGRSKAPVRRQPSPGLFDD
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQDIFPPPSDRLSGKARFADAHALAAALSRPIVFVDLETTGADAQHDRITEIGVVEVGPE
CCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEECHH
GIQEWDTLLDPGVSIPPFIQRLTGITDETVQGQPTFASIAEALAERLQGRLFVAHNARFD
HHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
YGFLKNEFRRAGLTFRADVLCTVRLSRSLFPSAARHGLDAIIARFDLQPRARHRALADAE
HHHHHHHHHHCCCCEEEEEEEEEHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLWQFWQKIHALYSVELIDAATGALVKRASLPAGLEETALDDVPDTAGVYLFYGDNDAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEECCCCCCE
YIGKSVHLRQRIGAHFSGDYRLGKDMRLARLVRRVEWRETGGEIGALLLEASLVKTMQPL
EECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
HNQMLRGSARLYTWELPPELPVPRLRSDADTDFSRRGNLYGAFASRGAAQAWLRRLADEH
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCEEEHHHCCCHHHHHHHHHHHHH
RLCQATLTLEKTARAGNPCFARQLHRCEGACVGEEASPSHRARIATAMAEARLKRWPFDG
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PAAWPEVSGTQTWWHVAEDWCYLGSVQTLEDVTTLLAAPARFDLDTYQILASRLQQWMPN
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
AVPLTSTRSFELALCEAPAPDDRSPPNLPQGPGRSKAPVRRQPSPGLFDD
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]