Click here to switch to the map view.

The map label for this gene is citE [H]

Identifier: 73537374

GI number: 73537374

Start: 86978

End: 87817

Strand: Reverse

Name: citE [H]

Synonym: Reut_B3539

Alternate gene names: 73537374

Gene position: 87817-86978 (Counterclockwise)

Preceding gene: 73537375

Following gene: 73537373

Centisome position: 3.22

GC content: 67.98

Gene sequence:

>840_bases
ATGTCCACTGCAGTCACCTACCTGTTTGTGCCGGGCGACCGGCCCGAACGCTTTGACAAGGCCGCCGCGGCGAAGCCCGA
TGTGATGGTCCTCGACCTCGAGGATGCCGTGCATCCGGACGCCAAGCCGGCGGCGCGCCAGGCCATTGCGGATTGGCTAG
CCGCGGGCGCCGCTGCCAATGCCAATGTACGCATCAATGACAGCGCCGCTGCGGGCTTCGCCGCCGACATCGAATGGCTG
CGCAGTTTGCCGAAGGGCACGCCATTGGCGGGCCTGATGGTGCCAAAGGCGGAAGACGCCGCGGCACTGGCTGCGATCAC
CGAGGTCCTGCGCGGGATCAATCCGGAAGCCGAGCTGATCGCCATCATCGAAACCGCGCTCGGGCTGCATCAGGTTGATG
CCGTGGCGACTGCCAACGGTGTTGCCCGGCTGGCATTCGGTTCGCTCGACTATGCGGTCGACCTGGGCTGCAGCCACACG
CGCGATGCGCTGGCCTATGCGCGTGCGCGTATCGTTCTGGCATCGCGCGTGGCTGGCCTGACGCCTCCCGTCGACGGCGT
CACCACGGCGCTGAAGGATGAGTCCGTCCTGGCCGACGACGTGGCCTATGCCCGCGCACTTGGCTTTGGCGGCAAGCTCT
GCATCCACCCGGCACAGCTCGGTGCGGTTCGTGCCGGCTTCCTGCCCTCGCCCGAGCAACTCGACTGGGCGCGGCGCGTG
CTGGAAGCGACCGCCAGCGGCAGCCATGCCGTGCAGGTGGACGGCAAGATGGTGGATCGCCCCGTCATCGAACAGGCGCG
TCGCCTGCTCGCGCTGGCGCAACCGTCAGCGGCACACTGA

Upstream 100 bases:

>100_bases
GCGACCGAGGCCGAGATGCGTGTGATCATTGCGCGCGTGTGGGCCCTGGAAAGCGCACCGACCGTCAACGACTGGCTGCC
CGCACGCTGAGGCACAGACC

Downstream 100 bases:

>100_bases
ACACGAAGGTAGTCGTCATGACCCAGAACTCCCAACTCGACCATCTGCGCACATGGATCGGCCGCAGCGAATCGCGCACC
GAGACACTGACGCCGGAACC

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSTAVTYLFVPGDRPERFDKAAAAKPDVMVLDLEDAVHPDAKPAARQAIADWLAAGAAANANVRINDSAAAGFAADIEWL
RSLPKGTPLAGLMVPKAEDAAALAAITEVLRGINPEAELIAIIETALGLHQVDAVATANGVARLAFGSLDYAVDLGCSHT
RDALAYARARIVLASRVAGLTPPVDGVTTALKDESVLADDVAYARALGFGGKLCIHPAQLGAVRAGFLPSPEQLDWARRV
LEATASGSHAVQVDGKMVDRPVIEQARRLLALAQPSAAH

Sequences:

>Translated_279_residues
MSTAVTYLFVPGDRPERFDKAAAAKPDVMVLDLEDAVHPDAKPAARQAIADWLAAGAAANANVRINDSAAAGFAADIEWL
RSLPKGTPLAGLMVPKAEDAAALAAITEVLRGINPEAELIAIIETALGLHQVDAVATANGVARLAFGSLDYAVDLGCSHT
RDALAYARARIVLASRVAGLTPPVDGVTTALKDESVLADDVAYARALGFGGKLCIHPAQLGAVRAGFLPSPEQLDWARRV
LEATASGSHAVQVDGKMVDRPVIEQARRLLALAQPSAAH
>Mature_278_residues
STAVTYLFVPGDRPERFDKAAAAKPDVMVLDLEDAVHPDAKPAARQAIADWLAAGAAANANVRINDSAAAGFAADIEWLR
SLPKGTPLAGLMVPKAEDAAALAAITEVLRGINPEAELIAIIETALGLHQVDAVATANGVARLAFGSLDYAVDLGCSHTR
DALAYARARIVLASRVAGLTPPVDGVTTALKDESVLADDVAYARALGFGGKLCIHPAQLGAVRAGFLPSPEQLDWARRVL
EATASGSHAVQVDGKMVDRPVIEQARRLLALAQPSAAH

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=294, Percent_Identity=30.2721088435374, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI87081764, Length=296, Percent_Identity=29.7297297297297, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17557458, Length=296, Percent_Identity=29.3918918918919, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 28999; Mature: 28868

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAVTYLFVPGDRPERFDKAAAAKPDVMVLDLEDAVHPDAKPAARQAIADWLAAGAAAN
CCCEEEEEEECCCCHHHHHHHHCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHHHCCCCC
ANVRINDSAAAGFAADIEWLRSLPKGTPLAGLMVPKAEDAAALAAITEVLRGINPEAELI
CCEEECCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHH
AIIETALGLHQVDAVATANGVARLAFGSLDYAVDLGCSHTRDALAYARARIVLASRVAGL
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
TPPVDGVTTALKDESVLADDVAYARALGFGGKLCIHPAQLGAVRAGFLPSPEQLDWARRV
CCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCHHHHHHHHH
LEATASGSHAVQVDGKMVDRPVIEQARRLLALAQPSAAH
HHHHCCCCEEEEECCCHHCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STAVTYLFVPGDRPERFDKAAAAKPDVMVLDLEDAVHPDAKPAARQAIADWLAAGAAAN
CCEEEEEEECCCCHHHHHHHHCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHHHCCCCC
ANVRINDSAAAGFAADIEWLRSLPKGTPLAGLMVPKAEDAAALAAITEVLRGINPEAELI
CCEEECCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHH
AIIETALGLHQVDAVATANGVARLAFGSLDYAVDLGCSHTRDALAYARARIVLASRVAGL
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
TPPVDGVTTALKDESVLADDVAYARALGFGGKLCIHPAQLGAVRAGFLPSPEQLDWARRV
CCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCHHHHHHHHH
LEATASGSHAVQVDGKMVDRPVIEQARRLLALAQPSAAH
HHHHCCCCEEEEECCCHHCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]