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The map label for this gene is dinB [H]

Identifier: 73543076

GI number: 73543076

Start: 3721531

End: 3722625

Strand: Reverse

Name: dinB [H]

Synonym: Reut_A3394

Alternate gene names: 73543076

Gene position: 3722625-3721531 (Counterclockwise)

Preceding gene: 73543077

Following gene: 73543073

Centisome position: 97.8

GC content: 66.76

Gene sequence:

>1095_bases
ATGGCTATCATGCCCGTCGACCCATCCACGCCCCCGGAAGCAGCCGTCCAGCGCAAGATCATCCATTGCGACTGCGACTG
CTTCTACGCGTCGGTCGAGATGCGGGATGACCCGTCGTTGCGGGGCCGCCCCATGGCGGTAGGCGGCTCCCCCGACCGCC
GCGGCGTGATCGCAACCTGCAACTACGAGGCGCGCGCGTTCGGCGTGCGCTCGGCCATGTCGTCGGCGCAGGCGGTGCGG
CGCTGCCCGGACCTGCTGATCCTGCGCCCGGCCATGGACAAATACCGCGAGGTGTCGCGGCGGATCTTTGCGATCTACCG
TGAATACACGGCGCTGGTGGAACCGCTTTCGCTGGATGAAGCCTACCTCGACGTAAGCCAGTGTCCGCAGCACGATGGCA
GCGCCACGCGCATCGCGGAGGAAATCCGGCGGCGTGTGCGCGAGGAGGTCGGTGTGACGGTGTCAGCGGGCGTCGGCCCG
AACAAGTTCGTCGCCAAGATCGCCAGCGACTGGAACAAGCCCGACGGGTTGTTCGTGGTACGGCCTGCTTCCGTCGACGT
ATTCGTCGCGGAACTGCCGGTCGGACGGATCCATGGCGTGGGGGCCGTTACGGCTGGCAAGCTCAAGCGGCTGGGCGCCG
AAACCTGCGGCGACCTGCGCGCATGGCCCGCTGACAGGTTGCACCGCGAGTTCGGCAGCTTCGGCGCGCGGCTCTACAAC
CTGTGCCGCGGCATTGACCACCGCACGGTTTCGCCCGAACGCGAACGCAAGTCGATCAGTGTCGAGGAAACGTATGCCGA
CGACCTGCCGGATCTGCAAGCGTGCCTGGCCGAACTGGAACCGCTGATCGCCATGCTGGAGGCCCGCATTGTGCGGGCCC
AGGCGCATGACACGATCGACAAGCTCACCGTGAAGCTGCGCTTCTCGGACTTCCGCCAGACCACCGTCGAATGCCGCAGC
CATGCACCGGACCGCGATACCTATGCGCGGCTGCTTGCCGAAGGCCATGCCCGGCGCGGCTTGCCTGTGCGGTTGCTCGG
GGTAGGGGTCGCGACCAGCGATCGCGACAGGACACAGCTTGAGCTGTTTCCGTGA

Upstream 100 bases:

>100_bases
TCATTACTGCGTCGGCAGTTTTCATCGACGCCATTAATCACACACAAGACAATCAACCCCTCACAATAATTCGCATCAAT
GGATCGGCATTGCTCGATCC

Downstream 100 bases:

>100_bases
TTGTCATTCACCCGACAAATTGTTTAATCAAAAAAACCACACCACTGATTTTTCGCACAAATAACTACTGCAGATCGTTC
CGCATGATCAGCAGCCATGG

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MAIMPVDPSTPPEAAVQRKIIHCDCDCFYASVEMRDDPSLRGRPMAVGGSPDRRGVIATCNYEARAFGVRSAMSSAQAVR
RCPDLLILRPAMDKYREVSRRIFAIYREYTALVEPLSLDEAYLDVSQCPQHDGSATRIAEEIRRRVREEVGVTVSAGVGP
NKFVAKIASDWNKPDGLFVVRPASVDVFVAELPVGRIHGVGAVTAGKLKRLGAETCGDLRAWPADRLHREFGSFGARLYN
LCRGIDHRTVSPERERKSISVEETYADDLPDLQACLAELEPLIAMLEARIVRAQAHDTIDKLTVKLRFSDFRQTTVECRS
HAPDRDTYARLLAEGHARRGLPVRLLGVGVATSDRDRTQLELFP

Sequences:

>Translated_364_residues
MAIMPVDPSTPPEAAVQRKIIHCDCDCFYASVEMRDDPSLRGRPMAVGGSPDRRGVIATCNYEARAFGVRSAMSSAQAVR
RCPDLLILRPAMDKYREVSRRIFAIYREYTALVEPLSLDEAYLDVSQCPQHDGSATRIAEEIRRRVREEVGVTVSAGVGP
NKFVAKIASDWNKPDGLFVVRPASVDVFVAELPVGRIHGVGAVTAGKLKRLGAETCGDLRAWPADRLHREFGSFGARLYN
LCRGIDHRTVSPERERKSISVEETYADDLPDLQACLAELEPLIAMLEARIVRAQAHDTIDKLTVKLRFSDFRQTTVECRS
HAPDRDTYARLLAEGHARRGLPVRLLGVGVATSDRDRTQLELFP
>Mature_363_residues
AIMPVDPSTPPEAAVQRKIIHCDCDCFYASVEMRDDPSLRGRPMAVGGSPDRRGVIATCNYEARAFGVRSAMSSAQAVRR
CPDLLILRPAMDKYREVSRRIFAIYREYTALVEPLSLDEAYLDVSQCPQHDGSATRIAEEIRRRVREEVGVTVSAGVGPN
KFVAKIASDWNKPDGLFVVRPASVDVFVAELPVGRIHGVGAVTAGKLKRLGAETCGDLRAWPADRLHREFGSFGARLYNL
CRGIDHRTVSPERERKSISVEETYADDLPDLQACLAELEPLIAMLEARIVRAQAHDTIDKLTVKLRFSDFRQTTVECRSH
APDRDTYARLLAEGHARRGLPVRLLGVGVATSDRDRTQLELFP

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI84043967, Length=303, Percent_Identity=31.023102310231, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI7706681, Length=304, Percent_Identity=30.9210526315789, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI154350220, Length=323, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI5729982, Length=319, Percent_Identity=28.8401253918495, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI7705344, Length=108, Percent_Identity=47.2222222222222, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786425, Length=342, Percent_Identity=48.5380116959064, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI1787432, Length=207, Percent_Identity=29.4685990338164, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI193205700, Length=413, Percent_Identity=29.2978208232446, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17537959, Length=309, Percent_Identity=28.1553398058252, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI193205702, Length=347, Percent_Identity=27.0893371757925, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115534089, Length=125, Percent_Identity=38.4, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324921, Length=275, Percent_Identity=25.8181818181818, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355641, Length=287, Percent_Identity=32.0557491289199, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24644984, Length=287, Percent_Identity=32.0557491289199, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI19923006, Length=301, Percent_Identity=27.906976744186, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24668444, Length=122, Percent_Identity=36.0655737704918, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40440; Mature: 40308

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIMPVDPSTPPEAAVQRKIIHCDCDCFYASVEMRDDPSLRGRPMAVGGSPDRRGVIATC
CEEECCCCCCCHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCEEEECCCCCCCCEEEEE
NYEARAFGVRSAMSSAQAVRRCPDLLILRPAMDKYREVSRRIFAIYREYTALVEPLSLDE
CCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AYLDVSQCPQHDGSATRIAEEIRRRVREEVGVTVSAGVGPNKFVAKIASDWNKPDGLFVV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHCCCCCCCEEEE
RPASVDVFVAELPVGRIHGVGAVTAGKLKRLGAETCGDLRAWPADRLHREFGSFGARLYN
ECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LCRGIDHRTVSPERERKSISVEETYADDLPDLQACLAELEPLIAMLEARIVRAQAHDTID
HHHCCCCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLTVKLRFSDFRQTTVECRSHAPDRDTYARLLAEGHARRGLPVRLLGVGVATSDRDRTQL
HEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEE
ELFP
EECC
>Mature Secondary Structure 
AIMPVDPSTPPEAAVQRKIIHCDCDCFYASVEMRDDPSLRGRPMAVGGSPDRRGVIATC
EEECCCCCCCHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCEEEECCCCCCCCEEEEE
NYEARAFGVRSAMSSAQAVRRCPDLLILRPAMDKYREVSRRIFAIYREYTALVEPLSLDE
CCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AYLDVSQCPQHDGSATRIAEEIRRRVREEVGVTVSAGVGPNKFVAKIASDWNKPDGLFVV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHCCCCCCCEEEE
RPASVDVFVAELPVGRIHGVGAVTAGKLKRLGAETCGDLRAWPADRLHREFGSFGARLYN
ECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LCRGIDHRTVSPERERKSISVEETYADDLPDLQACLAELEPLIAMLEARIVRAQAHDTID
HHHCCCCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLTVKLRFSDFRQTTVECRSHAPDRDTYARLLAEGHARRGLPVRLLGVGVATSDRDRTQL
HEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEE
ELFP
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]