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The map label for this gene is hupA [H]

Identifier: 73543074

GI number: 73543074

Start: 3719963

End: 3720241

Strand: Direct

Name: hupA [H]

Synonym: Reut_A3392

Alternate gene names: 73543074

Gene position: 3719963-3720241 (Clockwise)

Preceding gene: 73543072

Following gene: 73543075

Centisome position: 97.73

GC content: 59.14

Gene sequence:

>279_bases
ATGACGAAAACCGAACTGATCGACGCTATCGCTGCAGGTGTGGACGGTCTGACCAAGGCTAAGGCTGAACAGGCACTGAA
CGTGACGCTGTCGGCCATCATGGATGCCGTTGCCAACGGCGATACGCTGAGCCTGGTCGGCTTTGGCACGTTCAGCAAGG
GTGAGCGCGGCGAGCGCATGGCCCGCAACCCGCGCACTGGCGAAGAAATCAAGGTGGAAGCAGCCAAGACGGTGAAGTTC
AAGGCTGGCCAGAAGTTCAAGGACGCCGTCAACCAGTAA

Upstream 100 bases:

>100_bases
ACGGTATCATGTGGCCCGCGACGTCTTTGCCGGGCCGGCCCTGCCATGGGCTGCCCGTGCCCGGACGTCTGTGTTTTATC
AACTCGATACGGAGTGAAGC

Downstream 100 bases:

>100_bases
TCCCGGGCATGCCCGGCGGGTCGCTGCCGCGCCCCGGTTTTGCATGACCCCGCCATGCCGCCACCGTGTCGTCCAAGGAC
GCAGGTGCGCGCCCGGCGGG

Product: HU family DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 92; Mature: 91

Protein sequence:

>92_residues
MTKTELIDAIAAGVDGLTKAKAEQALNVTLSAIMDAVANGDTLSLVGFGTFSKGERGERMARNPRTGEEIKVEAAKTVKF
KAGQKFKDAVNQ

Sequences:

>Translated_92_residues
MTKTELIDAIAAGVDGLTKAKAEQALNVTLSAIMDAVANGDTLSLVGFGTFSKGERGERMARNPRTGEEIKVEAAKTVKF
KAGQKFKDAVNQ
>Mature_91_residues
TKTELIDAIAAGVDGLTKAKAEQALNVTLSAIMDAVANGDTLSLVGFGTFSKGERGERMARNPRTGEEIKVEAAKTVKFK
AGQKFKDAVNQ

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=91, Percent_Identity=49.4505494505495, Blast_Score=91, Evalue=1e-20,
Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=50, Blast_Score=86, Evalue=6e-19,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=32.967032967033, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=35.5555555555556, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9728; Mature: 9597

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTELIDAIAAGVDGLTKAKAEQALNVTLSAIMDAVANGDTLSLVGFGTFSKGERGERM
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
ARNPRTGEEIKVEAAKTVKFKAGQKFKDAVNQ
HCCCCCCCEEEEEHHHEEECCCCCHHHHHHCC
>Mature Secondary Structure 
TKTELIDAIAAGVDGLTKAKAEQALNVTLSAIMDAVANGDTLSLVGFGTFSKGERGERM
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
ARNPRTGEEIKVEAAKTVKFKAGQKFKDAVNQ
HCCCCCCCEEEEEHHHEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10419967 [H]