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The map label for this gene is alkJ [H]

Identifier: 73543054

GI number: 73543054

Start: 3691585

End: 3693204

Strand: Reverse

Name: alkJ [H]

Synonym: Reut_A3372

Alternate gene names: 73543054

Gene position: 3693204-3691585 (Counterclockwise)

Preceding gene: 73543056

Following gene: 73543053

Centisome position: 97.02

GC content: 65.86

Gene sequence:

>1620_bases
ATGGAGACCTTCGACTACATCATCGTGGGCGCCGGCTCGGCCGGCTGCGTGCTGGCCAACCGCCTCACGCAGGACGCGGA
TGTCAATGTGCTGCTGCTCGAGGCGGGCGGCAAGGACGATTATCACTGGATCCATATTCCGGTCGGCTATCTCTATTGCA
TCGGCAATCCGCGCACGGACTGGCTGTATCGCACGGTTGCGGAGAAGGGGCTCAACGGCCGTTCGCTCGGTTATCCGCGC
GGACGTGTGCTCGGGGGCTCGTCGTCGATCAACGGCATGATCTATATGCGCGGTCAGCGCGAGGACTATGACGACTGGGC
ACGCCTGAGCGGCGACGACGGCTGGCGCTGGGACAACGTGCTGCCACTGTTCAAGCGCAGCGAGGACCACCATCGCGGCC
CCAGCGAATTCCATGGCGCCGGCGGCGAATGGCGTGTGGAAGCGCAGCGCCTGCGCTGGGACATCCTTGAACGCTTTATC
GACGCCGCCGAGCAGGCCGGCATTCCGCGCACCGACGACTTCAACCGTGGCGACAACTTCGGCGTGGGGTATTTCGAGGT
CAACCAGCGCCGCGGCATCCGCTGGAATACGTCCAAGGCTTTCCTGCGGCGTGCGGCGGACCGGCCGAATCTGACTATCG
TGACGGGGGCGCAAGTCAGTGCGCTGACTTTCGAAGGGCGACGCTGCACGGGCGTGAACTACGTCGGTGGCGGGCAGGCG
CACAGCGCGGCGGCGCGGCACGAAGTGATCCTGGCCGCGGGCGCAGTCAACACGCCGCAATTGCTCGAGTTGTCAGGCAT
CGGGCAGGGCGAGCGCCTGCAGGCCCTGGGTATTCAGGTGCGGCATGCATTGCCGGGCGTGGGCGAGAACCTGCAGGACC
ACCTGCAGTTGCGCACCGTGGTCAAGGTCAATGGTGTGCGCACGCTGAATACGCGCGTGGCGAGTTGGTGGGGCAAGCTC
GGCATCGGGCTGGAATACGCATTCAACCAGAGCGGGCCGATGAGCATGGCGCCATCGCAACTTGGCGCGTTCGCGCGCTC
GGATGCGTCGCAGGCGCGGCCAAACCTGGAGTACCACGTTCAACCGCTGTCGCTCGACAAGTTCGGCGAGCCGCTGCACG
CGTTCAACGCATTCACGGCCAGCGTCTGCAACCTGCGCCCGACCTCGCGCGGCAGCGTCCATATCGATAGCGCTGATTTC
CGGCAGGCGCCGGTGATCGCGCCCAACTATCTGTCGACGGACGAGGACCGCAAGGTTGCAGCCGATTCGTTGCGGCTCAC
GCGTCGTATCGTGGCGTCGCCCGCCCTGGCGCCGTATCGCCCGGAGGAGTGGTTGCCCGGCCCTGGCTTCGAGACGGACG
AAGCGCTGGCCGAGGCCGCGGGCAATATCGGCACCACGATCTTCCATCCGGTCGGCACATGCCGCATGGGCCGCCCGGAC
GATCCGCTTGCCGTCGTGGATCACCGGCTGCGCGTACATGGGATCGAGGGACTTCGCGTGGTCGATGCCTCGGTCATGCC
GCTGATTACATCGGGCAACACCAATTCGCCGACCATCATGATTGCCGAGCGGGCGAGCGACATGCTGCGCGAGGAGCGCC
GCCAGCAACTCGCTGCATGA

Upstream 100 bases:

>100_bases
GCATCTTCACTGAACGCAAAGATGGTCGTCCTCCGGGACGCGCATTTGCCCTGCCAGACACCAACATAACAAGAACAAGC
ACGGAGACGAGACGACTCGT

Downstream 100 bases:

>100_bases
CATGGCGCGCACGCGGAAAGGGCAGGGCGGGGCGCCGATCGCCCGCAAGTCCTTGATGCAGCGCGTCAAAAACCTGACAC
GTGGTTCATGTCTTATAGTG

Product: glucose-methanol-choline oxidoreductase:FAD dependent oxidoreductase:GMC oxidoreductase

Products: betaine aldehyde; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
METFDYIIVGAGSAGCVLANRLTQDADVNVLLLEAGGKDDYHWIHIPVGYLYCIGNPRTDWLYRTVAEKGLNGRSLGYPR
GRVLGGSSSINGMIYMRGQREDYDDWARLSGDDGWRWDNVLPLFKRSEDHHRGPSEFHGAGGEWRVEAQRLRWDILERFI
DAAEQAGIPRTDDFNRGDNFGVGYFEVNQRRGIRWNTSKAFLRRAADRPNLTIVTGAQVSALTFEGRRCTGVNYVGGGQA
HSAAARHEVILAAGAVNTPQLLELSGIGQGERLQALGIQVRHALPGVGENLQDHLQLRTVVKVNGVRTLNTRVASWWGKL
GIGLEYAFNQSGPMSMAPSQLGAFARSDASQARPNLEYHVQPLSLDKFGEPLHAFNAFTASVCNLRPTSRGSVHIDSADF
RQAPVIAPNYLSTDEDRKVAADSLRLTRRIVASPALAPYRPEEWLPGPGFETDEALAEAAGNIGTTIFHPVGTCRMGRPD
DPLAVVDHRLRVHGIEGLRVVDASVMPLITSGNTNSPTIMIAERASDMLREERRQQLAA

Sequences:

>Translated_539_residues
METFDYIIVGAGSAGCVLANRLTQDADVNVLLLEAGGKDDYHWIHIPVGYLYCIGNPRTDWLYRTVAEKGLNGRSLGYPR
GRVLGGSSSINGMIYMRGQREDYDDWARLSGDDGWRWDNVLPLFKRSEDHHRGPSEFHGAGGEWRVEAQRLRWDILERFI
DAAEQAGIPRTDDFNRGDNFGVGYFEVNQRRGIRWNTSKAFLRRAADRPNLTIVTGAQVSALTFEGRRCTGVNYVGGGQA
HSAAARHEVILAAGAVNTPQLLELSGIGQGERLQALGIQVRHALPGVGENLQDHLQLRTVVKVNGVRTLNTRVASWWGKL
GIGLEYAFNQSGPMSMAPSQLGAFARSDASQARPNLEYHVQPLSLDKFGEPLHAFNAFTASVCNLRPTSRGSVHIDSADF
RQAPVIAPNYLSTDEDRKVAADSLRLTRRIVASPALAPYRPEEWLPGPGFETDEALAEAAGNIGTTIFHPVGTCRMGRPD
DPLAVVDHRLRVHGIEGLRVVDASVMPLITSGNTNSPTIMIAERASDMLREERRQQLAA
>Mature_539_residues
METFDYIIVGAGSAGCVLANRLTQDADVNVLLLEAGGKDDYHWIHIPVGYLYCIGNPRTDWLYRTVAEKGLNGRSLGYPR
GRVLGGSSSINGMIYMRGQREDYDDWARLSGDDGWRWDNVLPLFKRSEDHHRGPSEFHGAGGEWRVEAQRLRWDILERFI
DAAEQAGIPRTDDFNRGDNFGVGYFEVNQRRGIRWNTSKAFLRRAADRPNLTIVTGAQVSALTFEGRRCTGVNYVGGGQA
HSAAARHEVILAAGAVNTPQLLELSGIGQGERLQALGIQVRHALPGVGENLQDHLQLRTVVKVNGVRTLNTRVASWWGKL
GIGLEYAFNQSGPMSMAPSQLGAFARSDASQARPNLEYHVQPLSLDKFGEPLHAFNAFTASVCNLRPTSRGSVHIDSADF
RQAPVIAPNYLSTDEDRKVAADSLRLTRRIVASPALAPYRPEEWLPGPGFETDEALAEAAGNIGTTIFHPVGTCRMGRPD
DPLAVVDHRLRVHGIEGLRVVDASVMPLITSGNTNSPTIMIAERASDMLREERRQQLAA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=536, Percent_Identity=42.3507462686567, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1786503, Length=534, Percent_Identity=41.5730337078652, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17532301, Length=549, Percent_Identity=36.0655737704918, Blast_Score=347, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24642055, Length=576, Percent_Identity=36.4583333333333, Blast_Score=314, Evalue=9e-86,
Organism=Drosophila melanogaster, GI24642042, Length=570, Percent_Identity=36.6666666666667, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24642048, Length=576, Percent_Identity=35.0694444444444, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI45549471, Length=569, Percent_Identity=35.1493848857645, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI45551458, Length=569, Percent_Identity=35.1493848857645, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24642059, Length=573, Percent_Identity=34.9040139616056, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24650267, Length=578, Percent_Identity=34.4290657439446, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24642035, Length=577, Percent_Identity=33.6221837088388, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI17137792, Length=564, Percent_Identity=33.6879432624113, Blast_Score=272, Evalue=5e-73,
Organism=Drosophila melanogaster, GI18859995, Length=563, Percent_Identity=34.6358792184725, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24642039, Length=566, Percent_Identity=33.5689045936396, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24642037, Length=573, Percent_Identity=32.4607329842932, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24642051, Length=570, Percent_Identity=31.4035087719298, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI18859993, Length=570, Percent_Identity=31.2280701754386, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642057, Length=589, Percent_Identity=30.5602716468591, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24645930, Length=589, Percent_Identity=29.7113752122241, Blast_Score=165, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1

Molecular weight: Translated: 59278; Mature: 59278

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFDYIIVGAGSAGCVLANRLTQDADVNVLLLEAGGKDDYHWIHIPVGYLYCIGNPRTD
CCCEEEEEEECCCCCCHHHHHCCCCCCCCEEEEECCCCCCEEEEEEEEEEEEECCCCCHH
WLYRTVAEKGLNGRSLGYPRGRVLGGSSSINGMIYMRGQREDYDDWARLSGDDGWRWDNV
HHHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCC
LPLFKRSEDHHRGPSEFHGAGGEWRVEAQRLRWDILERFIDAAEQAGIPRTDDFNRGDNF
CHHHHCCCCCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GVGYFEVNQRRGIRWNTSKAFLRRAADRPNLTIVTGAQVSALTFEGRRCTGVNYVGGGQA
CEEEEEECCCCCCEECHHHHHHHHHCCCCCEEEEECCEEEEEEECCCEECCCCCCCCCCH
HSAAARHEVILAAGAVNTPQLLELSGIGQGERLQALGIQVRHALPGVGENLQDHLQLRTV
HHHHHHCEEEEEECCCCCCCEEEECCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHEEEE
VKVNGVRTLNTRVASWWGKLGIGLEYAFNQSGPMSMAPSQLGAFARSDASQARPNLEYHV
EEECCEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCHHHCCCCCEEEE
QPLSLDKFGEPLHAFNAFTASVCNLRPTSRGSVHIDSADFRQAPVIAPNYLSTDEDRKVA
EECCHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEECCCCCCCCCCHHHH
ADSLRLTRRIVASPALAPYRPEEWLPGPGFETDEALAEAAGNIGTTIFHPVGTCRMGRPD
HHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCC
DPLAVVDHRLRVHGIEGLRVVDASVMPLITSGNTNSPTIMIAERASDMLREERRQQLAA
CCHHHHCCEEEEECCCCEEEECCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
METFDYIIVGAGSAGCVLANRLTQDADVNVLLLEAGGKDDYHWIHIPVGYLYCIGNPRTD
CCCEEEEEEECCCCCCHHHHHCCCCCCCCEEEEECCCCCCEEEEEEEEEEEEECCCCCHH
WLYRTVAEKGLNGRSLGYPRGRVLGGSSSINGMIYMRGQREDYDDWARLSGDDGWRWDNV
HHHHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCC
LPLFKRSEDHHRGPSEFHGAGGEWRVEAQRLRWDILERFIDAAEQAGIPRTDDFNRGDNF
CHHHHCCCCCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GVGYFEVNQRRGIRWNTSKAFLRRAADRPNLTIVTGAQVSALTFEGRRCTGVNYVGGGQA
CEEEEEECCCCCCEECHHHHHHHHHCCCCCEEEEECCEEEEEEECCCEECCCCCCCCCCH
HSAAARHEVILAAGAVNTPQLLELSGIGQGERLQALGIQVRHALPGVGENLQDHLQLRTV
HHHHHHCEEEEEECCCCCCCEEEECCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHHEEEE
VKVNGVRTLNTRVASWWGKLGIGLEYAFNQSGPMSMAPSQLGAFARSDASQARPNLEYHV
EEECCEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCHHHCCCCCEEEE
QPLSLDKFGEPLHAFNAFTASVCNLRPTSRGSVHIDSADFRQAPVIAPNYLSTDEDRKVA
EECCHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEECCCCCCCCCCHHHH
ADSLRLTRRIVASPALAPYRPEEWLPGPGFETDEALAEAAGNIGTTIFHPVGTCRMGRPD
HHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCC
DPLAVVDHRLRVHGIEGLRVVDASVMPLITSGNTNSPTIMIAERASDMLREERRQQLAA
CCHHHHCCEEEEECCCCEEEECCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: choline; acceptor

Specific reaction: choline + acceptor = betaine aldehyde + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]