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The map label for this gene is mpl [H]

Identifier: 73542546

GI number: 73542546

Start: 3134604

End: 3135977

Strand: Reverse

Name: mpl [H]

Synonym: Reut_A2861

Alternate gene names: 73542546

Gene position: 3135977-3134604 (Counterclockwise)

Preceding gene: 73542557

Following gene: 73542545

Centisome position: 82.38

GC content: 66.38

Gene sequence:

>1374_bases
ATGCATATACATATCCTTGGCATCTGCGGCACTTTCATGGGCGGCTTGGCGGTGCTCGCCAAGCAGGCGGGTCACCGTGT
CACGGGCTGCGATGCCAATGTCTATCCCCCGATGAGCACGCAGCTCGAAGCCCAGGGCATCGAACTGATCGAGGGCTTCG
ATCCGGCCCAGCTCTCGCTCGAGCCGGACCTGTTCGTGATCGGCAACGTGGTGTCGCGCGGCAATCCGCTGATGGAAGCC
ATCCTGAACCGCAACCTGCCGTATGTGTCGGGCCCGCAATGGCTTGGCGAACATGTGCTCGCCGGCAAGTGGGTGCTGGC
CGTAGCCGGCACGCACGGCAAGACCACCACCACGTCGATGCTCGCGTGGATCCTCGAAGACGCCGGCTACAACCCCGGCT
TCCTCGTAGGTGGCGTGCCGCAGAACTTCGGCATTTCGGCGCGCGTGACGGAGTCGGATTTCTTCGTGATCGAGGCGGAT
GAATACGACACCGCCTTCTTCGACAAGCGCAGCAAGTTCGTTCACTACCGCCCGCGCACGGCCATCCTGAACAACCTCGA
ATACGATCACGCCGACATCTTCCCTGATCTCGACGCGATCGAAACGCAGTTCCACCATCTCGTGCGCACGGTGCCGGGGC
AGGGGCGTCTTGTCGTCAATGGCCTCGAAGCTAGCCTGGCGCGCGTGATCGAGCGCGGTTGCTGGAGCGAAGTCGAGCAG
TTCGGCGTCGGTGACTGGCGCGAAAGCGACGCCGGCACCGACGATGGCAAGGACGCGTTCGACGTGTGGTTCGGCGACGC
CGTCCAGGGCCGCGTGAGCTGGGACCTGCAGGGCACCCATAACCGCATGAATGCGTTGGCCGCGATTGCCGCTGCGCGCC
ACGTCGGCGTGCCGGTTGCGCAGGCGATCGAATCGCTGGGCCGCTTCGCCAATGTGAAGCGCCGCATGGAAGTGCGCGGC
GTGGCGGCAGGTGTGACGGTCTATGACGACTTCGCCCACCATCCGACCGCGATCCAGACCACACTGGACGGCCTGCGCCG
CCGTGTCGGCGGTGCGCGCATCCTGGCCGTGCTGGAGCCGCGCTCGAACACCATGAAGCTCGGCGTGATGAAGGCGCAGT
TGCCGGCCAGCCTCGAGCAGGCCGACCTGGTGTTCGGCTACGGTGCGCCGAGCGGCAAGGACGCGCTCGGCTGGGACCTG
GCCGGCGCGCTGGCGCCGCTGGGCAAGAAGGCGGCGGCGTTCCACGATCTCGGCGCACTGGTCGAGGCCGTGCGCGCTGC
CGCACAGCCGGGCGACCAGGTGCTGGTCATGAGCAACGGCGGCTTCGGCGGCGTGCACCAGAAGCTGCTCGACGCGTTGG
GTAAGGCAGGCTGA

Upstream 100 bases:

>100_bases
TGCTCGGGTACAATCAGGACCTTCCTGCACGGCATGGTCCGACGGATCATCGCTGGCATGCCGACCGGCCCGGCCGGTCT
CAAGTCTTTCCGGGAACCCC

Downstream 100 bases:

>100_bases
GGACACCGACATGCTGTTGTACCTGCACGGATTCCGCTCCTCGCCGCAGTCGTTCAAGGCGCGGCTGGTGCAGGAGCGCA
TGCGCGAGTGGGGCGTGGGC

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MHIHILGICGTFMGGLAVLAKQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSLEPDLFVIGNVVSRGNPLMEA
ILNRNLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTTSMLAWILEDAGYNPGFLVGGVPQNFGISARVTESDFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLDAIETQFHHLVRTVPGQGRLVVNGLEASLARVIERGCWSEVEQ
FGVGDWRESDAGTDDGKDAFDVWFGDAVQGRVSWDLQGTHNRMNALAAIAAARHVGVPVAQAIESLGRFANVKRRMEVRG
VAAGVTVYDDFAHHPTAIQTTLDGLRRRVGGARILAVLEPRSNTMKLGVMKAQLPASLEQADLVFGYGAPSGKDALGWDL
AGALAPLGKKAAAFHDLGALVEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGKAG

Sequences:

>Translated_457_residues
MHIHILGICGTFMGGLAVLAKQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSLEPDLFVIGNVVSRGNPLMEA
ILNRNLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTTSMLAWILEDAGYNPGFLVGGVPQNFGISARVTESDFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLDAIETQFHHLVRTVPGQGRLVVNGLEASLARVIERGCWSEVEQ
FGVGDWRESDAGTDDGKDAFDVWFGDAVQGRVSWDLQGTHNRMNALAAIAAARHVGVPVAQAIESLGRFANVKRRMEVRG
VAAGVTVYDDFAHHPTAIQTTLDGLRRRVGGARILAVLEPRSNTMKLGVMKAQLPASLEQADLVFGYGAPSGKDALGWDL
AGALAPLGKKAAAFHDLGALVEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGKAG
>Mature_457_residues
MHIHILGICGTFMGGLAVLAKQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSLEPDLFVIGNVVSRGNPLMEA
ILNRNLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTTSMLAWILEDAGYNPGFLVGGVPQNFGISARVTESDFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEYDHADIFPDLDAIETQFHHLVRTVPGQGRLVVNGLEASLARVIERGCWSEVEQ
FGVGDWRESDAGTDDGKDAFDVWFGDAVQGRVSWDLQGTHNRMNALAAIAAARHVGVPVAQAIESLGRFANVKRRMEVRG
VAAGVTVYDDFAHHPTAIQTTLDGLRRRVGGARILAVLEPRSNTMKLGVMKAQLPASLEQADLVFGYGAPSGKDALGWDL
AGALAPLGKKAAAFHDLGALVEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGKAG

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=457, Percent_Identity=60.6126914660831, Blast_Score=540, Evalue=1e-154,
Organism=Escherichia coli, GI1786279, Length=359, Percent_Identity=28.6908077994429, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48975; Mature: 48975

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAVLAKQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEC
EPDLFVIGNVVSRGNPLMEAILNRNLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTTSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEDAGYNPGFLVGGVPQNFGISARVTESDFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEYDHADIFPDLDAIETQFHHLVRTVPGQGRLVVNGLEASLARVIERGCWSEVEQ
HHHHCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
FGVGDWRESDAGTDDGKDAFDVWFGDAVQGRVSWDLQGTHNRMNALAAIAAARHVGVPVA
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHH
QAIESLGRFANVKRRMEVRGVAAGVTVYDDFAHHPTAIQTTLDGLRRRVGGARILAVLEP
HHHHHHHHHHHHHHHHHHHHEEECEEEEHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECC
RSNTMKLGVMKAQLPASLEQADLVFGYGAPSGKDALGWDLAGALAPLGKKAAAFHDLGAL
CCCCEEEEEEEHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
VEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGKAG
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAVLAKQAGHRVTGCDANVYPPMSTQLEAQGIELIEGFDPAQLSL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEC
EPDLFVIGNVVSRGNPLMEAILNRNLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTTSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEDAGYNPGFLVGGVPQNFGISARVTESDFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEYDHADIFPDLDAIETQFHHLVRTVPGQGRLVVNGLEASLARVIERGCWSEVEQ
HHHHCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
FGVGDWRESDAGTDDGKDAFDVWFGDAVQGRVSWDLQGTHNRMNALAAIAAARHVGVPVA
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHH
QAIESLGRFANVKRRMEVRGVAAGVTVYDDFAHHPTAIQTTLDGLRRRVGGARILAVLEP
HHHHHHHHHHHHHHHHHHHHEEECEEEEHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECC
RSNTMKLGVMKAQLPASLEQADLVFGYGAPSGKDALGWDLAGALAPLGKKAAAFHDLGAL
CCCCEEEEEEEHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
VEAVRAAAQPGDQVLVMSNGGFGGVHQKLLDALGKAG
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]