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The map label for this gene is braE [H]

Identifier: 73542412

GI number: 73542412

Start: 2993395

End: 2994552

Strand: Reverse

Name: braE [H]

Synonym: Reut_A2727

Alternate gene names: 73542412

Gene position: 2994552-2993395 (Counterclockwise)

Preceding gene: 73542413

Following gene: 73542411

Centisome position: 78.67

GC content: 64.08

Gene sequence:

>1158_bases
ATGAATACTCCGATTCCATCCGCGGCCGCCGTCGCTGCGCCGTCCCGAGTTCCGGCGAAGACCCTGGCCGCGCTGCTGGG
CTTCCTGGTGATCGCGCTGTGCGCCCCGTTCTTCGTCCAGACGCTGGGCGGCAACTACTGGGTGCGCGTGCTCGATTTCG
CGCTGCTGTACATCATGCTGGCGCTGGGCCTGAACATCGTGGTGGGCTTTGCCGGCCTGCTTGACCTCGGCTATATCGCG
TTCTACGCGGTGGGTGCCTACATGATGGCGCTGCTCGGCTCACCCCACCTGGCCAACCAGTTCGAGTGGATCCATCAACT
GTTCCCCCACGGGCTGCACTTGTCGATGTGGTGGGTGTTGCCGCTTGCGGTGCTGGTGGCGGCCACGTTCGGCGTGCTGC
TTGGCGCGCCAACGCTGAAGCTGCGCGGTGACTACCTCGCCATCGTGACGCTTGGCTTCGGCGAGATCATCCGCATCTTC
ATGAACAACCTGGACCGCCCGCTGAATATCACCAACGGGCCGAAGGGCATCACGGCGGTGGACCCGGTCCACATCTTCGG
CTTCGACTTCTCGAAGACGCACGAGATTTTCGGGCTGAAATTCTCGCCGGTGTTCATGTACTACTACCTGCTCGTGTTCC
TGGTCATCGTGATCGTGTTTGTGTGCCTGCGCCTGCAGAACTCGCGCATCGGCCGTGCGTTCGTGGCGATCCGCGAAGAC
GAGATCGCCGCCAAGGCGATGGGCATCAACACCCGCAACATCAAGCTGCTGGCCTTCGCCATGGGCGCATCGTTCGGCGG
CGCCTCGGGCGCGGTGTTCGGTGCGTTCCAGGGCTTCGTGTCGCCGGAATCGTTCACGCTGTGGGAGTCGATCTACATCC
TGGCCATCGTGGTGCTGGGCGGCATGGGCCATATCCCGGGCGTGATCCTGGGCGGCATCCTGCTGGTCGGCTTCCAGGAG
TTGCTGCGCGTGGTCGCCGAGCCGATCCAGACCGGTATCTTCGGTCACGTGATCGTTGACGCCGAAGTCCTGCGCCAGTT
GCTGTTCGGCCTGGCCCTGGTTGGTGTGATGCTTTACCGCCCGGCAGGTATCTGGCCGTCGCCGCGCAAGGAAGACCGCC
CGGTGGTGCGCCGTGCGGGCAGTGTGGGCCGTTTCTGA

Upstream 100 bases:

>100_bases
GGACGTCTTTGCGTTCATTGTGCTGATTACCGTACTTGTGTTCCGTCCGTCCGGCATCATGGGCGAACGCGTGGCTGACC
GTGCCTGAGGGAGCGAATCC

Downstream 100 bases:

>100_bases
CCAGGAGGACAACATGAGCAACAACGATTTCCTCCTGTCGGTACAGGGGGTCAACAAGCGATTCGGCGGCCTGCAGGCGC
TGTCCGATGTGGGCCTGCAG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MNTPIPSAAAVAAPSRVPAKTLAALLGFLVIALCAPFFVQTLGGNYWVRVLDFALLYIMLALGLNIVVGFAGLLDLGYIA
FYAVGAYMMALLGSPHLANQFEWIHQLFPHGLHLSMWWVLPLAVLVAATFGVLLGAPTLKLRGDYLAIVTLGFGEIIRIF
MNNLDRPLNITNGPKGITAVDPVHIFGFDFSKTHEIFGLKFSPVFMYYYLLVFLVIVIVFVCLRLQNSRIGRAFVAIRED
EIAAKAMGINTRNIKLLAFAMGASFGGASGAVFGAFQGFVSPESFTLWESIYILAIVVLGGMGHIPGVILGGILLVGFQE
LLRVVAEPIQTGIFGHVIVDAEVLRQLLFGLALVGVMLYRPAGIWPSPRKEDRPVVRRAGSVGRF

Sequences:

>Translated_385_residues
MNTPIPSAAAVAAPSRVPAKTLAALLGFLVIALCAPFFVQTLGGNYWVRVLDFALLYIMLALGLNIVVGFAGLLDLGYIA
FYAVGAYMMALLGSPHLANQFEWIHQLFPHGLHLSMWWVLPLAVLVAATFGVLLGAPTLKLRGDYLAIVTLGFGEIIRIF
MNNLDRPLNITNGPKGITAVDPVHIFGFDFSKTHEIFGLKFSPVFMYYYLLVFLVIVIVFVCLRLQNSRIGRAFVAIRED
EIAAKAMGINTRNIKLLAFAMGASFGGASGAVFGAFQGFVSPESFTLWESIYILAIVVLGGMGHIPGVILGGILLVGFQE
LLRVVAEPIQTGIFGHVIVDAEVLRQLLFGLALVGVMLYRPAGIWPSPRKEDRPVVRRAGSVGRF
>Mature_385_residues
MNTPIPSAAAVAAPSRVPAKTLAALLGFLVIALCAPFFVQTLGGNYWVRVLDFALLYIMLALGLNIVVGFAGLLDLGYIA
FYAVGAYMMALLGSPHLANQFEWIHQLFPHGLHLSMWWVLPLAVLVAATFGVLLGAPTLKLRGDYLAIVTLGFGEIIRIF
MNNLDRPLNITNGPKGITAVDPVHIFGFDFSKTHEIFGLKFSPVFMYYYLLVFLVIVIVFVCLRLQNSRIGRAFVAIRED
EIAAKAMGINTRNIKLLAFAMGASFGGASGAVFGAFQGFVSPESFTLWESIYILAIVVLGGMGHIPGVILGGILLVGFQE
LLRVVAEPIQTGIFGHVIVDAEVLRQLLFGLALVGVMLYRPAGIWPSPRKEDRPVVRRAGSVGRF

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=337, Percent_Identity=44.5103857566766, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 41779; Mature: 41779

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPIPSAAAVAAPSRVPAKTLAALLGFLVIALCAPFFVQTLGGNYWVRVLDFALLYIML
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALGLNIVVGFAGLLDLGYIAFYAVGAYMMALLGSPHLANQFEWIHQLFPHGLHLSMWWVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHH
PLAVLVAATFGVLLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLDRPLNITNGPKGITAV
HHHHHHHHHHHHHHCCCCEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
DPVHIFGFDFSKTHEIFGLKFSPVFMYYYLLVFLVIVIVFVCLRLQNSRIGRAFVAIRED
CCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEHH
EIAAKAMGINTRNIKLLAFAMGASFGGASGAVFGAFQGFVSPESFTLWESIYILAIVVLG
HHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GMGHIPGVILGGILLVGFQELLRVVAEPIQTGIFGHVIVDAEVLRQLLFGLALVGVMLYR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECHHHHHHHHHHHHHHHHHHHC
PAGIWPSPRKEDRPVVRRAGSVGRF
CCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MNTPIPSAAAVAAPSRVPAKTLAALLGFLVIALCAPFFVQTLGGNYWVRVLDFALLYIML
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ALGLNIVVGFAGLLDLGYIAFYAVGAYMMALLGSPHLANQFEWIHQLFPHGLHLSMWWVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHH
PLAVLVAATFGVLLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLDRPLNITNGPKGITAV
HHHHHHHHHHHHHHCCCCEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
DPVHIFGFDFSKTHEIFGLKFSPVFMYYYLLVFLVIVIVFVCLRLQNSRIGRAFVAIRED
CCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEHH
EIAAKAMGINTRNIKLLAFAMGASFGGASGAVFGAFQGFVSPESFTLWESIYILAIVVLG
HHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GMGHIPGVILGGILLVGFQELLRVVAEPIQTGIFGHVIVDAEVLRQLLFGLALVGVMLYR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECHHHHHHHHHHHHHHHHHHHC
PAGIWPSPRKEDRPVVRRAGSVGRF
CCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]