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The map label for this gene is cysD [H]

Identifier: 73542378

GI number: 73542378

Start: 2955681

End: 2956646

Strand: Reverse

Name: cysD [H]

Synonym: Reut_A2693

Alternate gene names: 73542378

Gene position: 2956646-2955681 (Counterclockwise)

Preceding gene: 73542379

Following gene: 73542377

Centisome position: 77.67

GC content: 64.91

Gene sequence:

>966_bases
ATGGGCATCATGAACGACATCGCAAGCGCCACCAGCAGCGTGGCGCACTTGCTGCAGGTACAGAACGATCACCTCGACCG
CCTGGAGGCCGAGTCGATCTACATCATCCGCGAGGTCGTGGCCGAGTGCCGCAACCCGGCGCTGCTGTTTTCGGGCGGCA
AGGATTCGATCGTCATGCTGCACCTGGCATTGAAGGCCTTCCGCCTCGGTGACCGCAAGATCGAGCTGCCGTTCCCGCTC
GTGCACATCGACACCGGCCACAACTATCCGGAAGTGATCCAGTTCCGCGACCAGCGCGTGGCCGAACTCGGCGCGCGCCT
GGTGGTGGGCCATGTGGAGGATTCCATGAAGCGCGGCACCGTGCGCCTGCGCAAGGAAACCGATTCGCGCAACGCCGCGC
AGGCCGTGACGCTGCTCGAAACCATCGAGGCGCACCAGTTCGACGCGCTGATGGGCGGTGCCCGCCGCGACGAAGAGAAG
GCCCGCGCCAAGGAACGCATCTTCTCGTTCCGCGATGAATTCGGCCAGTGGGACCCGAAGGCCCAGCGCCCCGAACTGTG
GAGCCTGTACAACGCCCGCATGGCGCAGGGCGAGCAGATGCGCGTGTTCCCGATCTCGAACTGGACCGAACTCGACGTGT
GGCAGTACATCGCCCGCGAGAAGCTGGCCCTGCCGCCGATCTACTACGCGCACCAGCGTGAAGTCGTGCGCAAGAACGGC
CTGCTGGTGCCGGTGACGCCGATCACGCCGAAGCAAGATGGTGACGTCAGCGAAACGTTGTCGGTCCGCTTCCGTACCGT
CGGCGACATCAGCTGCACCTGCCCGGTGGCCAGCGAGGCCGATTCGCCCGAAGCGATCATCGCCGAAACCGCGGTGACCG
AAATCACCGAGCGGGGTGCCACGCGCATGGACGACCAGACCAGCGAGGCGTCGATGGAGCGCCGCAAGAAGGAAGGCTAT
TTCTAA

Upstream 100 bases:

>100_bases
GCCGGGCGCTGGTGGTGGGAGAGCAAGGACTCGAAAGAGTGCGGGCTCCACGAACAGAACATCAAGCATTGACGCATTGA
AGCATTCGAGGCCGAACGAC

Downstream 100 bases:

>100_bases
GCCGACGCAGCCAAGGATTCAGAACATGACTCACCAATCTACCCATCAGGGCCTGCTGCGTTTCATCACCGCGGGTTCCG
TCGACGACGGCAAGAGCACG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MGIMNDIASATSSVAHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKIELPFPL
VHIDTGHNYPEVIQFRDQRVAELGARLVVGHVEDSMKRGTVRLRKETDSRNAAQAVTLLETIEAHQFDALMGGARRDEEK
ARAKERIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIAREKLALPPIYYAHQREVVRKNG
LLVPVTPITPKQDGDVSETLSVRFRTVGDISCTCPVASEADSPEAIIAETAVTEITERGATRMDDQTSEASMERRKKEGY
F

Sequences:

>Translated_321_residues
MGIMNDIASATSSVAHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKIELPFPL
VHIDTGHNYPEVIQFRDQRVAELGARLVVGHVEDSMKRGTVRLRKETDSRNAAQAVTLLETIEAHQFDALMGGARRDEEK
ARAKERIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIAREKLALPPIYYAHQREVVRKNG
LLVPVTPITPKQDGDVSETLSVRFRTVGDISCTCPVASEADSPEAIIAETAVTEITERGATRMDDQTSEASMERRKKEGY
F
>Mature_320_residues
GIMNDIASATSSVAHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKIELPFPLV
HIDTGHNYPEVIQFRDQRVAELGARLVVGHVEDSMKRGTVRLRKETDSRNAAQAVTLLETIEAHQFDALMGGARRDEEKA
RAKERIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIAREKLALPPIYYAHQREVVRKNGL
LVPVTPITPKQDGDVSETLSVRFRTVGDISCTCPVASEADSPEAIIAETAVTEITERGATRMDDQTSEASMERRKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=305, Percent_Identity=45.5737704918033, Blast_Score=255, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36351; Mature: 36220

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIMNDIASATSSVAHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVML
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHH
HLALKAFRLGDRKIELPFPLVHIDTGHNYPEVIQFRDQRVAELGARLVVGHVEDSMKRGT
HHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCHHEEEEHHHHHHHHCC
VRLRKETDSRNAAQAVTLLETIEAHQFDALMGGARRDEEKARAKERIFSFRDEFGQWDPK
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIAREKLALPPIYYAHQREVVRKNG
CCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
LLVPVTPITPKQDGDVSETLSVRFRTVGDISCTCPVASEADSPEAIIAETAVTEITERGA
CEEEECCCCCCCCCCHHHHHHEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
TRMDDQTSEASMERRKKEGYF
CCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GIMNDIASATSSVAHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVML
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHH
HLALKAFRLGDRKIELPFPLVHIDTGHNYPEVIQFRDQRVAELGARLVVGHVEDSMKRGT
HHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCHHEEEEHHHHHHHHCC
VRLRKETDSRNAAQAVTLLETIEAHQFDALMGGARRDEEKARAKERIFSFRDEFGQWDPK
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIAREKLALPPIYYAHQREVVRKNG
CCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
LLVPVTPITPKQDGDVSETLSVRFRTVGDISCTCPVASEADSPEAIIAETAVTEITERGA
CEEEECCCCCCCCCCHHHHHHEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
TRMDDQTSEASMERRKKEGYF
CCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]