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The map label for this gene is ynfJ [C]

Identifier: 73542359

GI number: 73542359

Start: 2937355

End: 2939109

Strand: Reverse

Name: ynfJ [C]

Synonym: Reut_A2674

Alternate gene names: 73542359

Gene position: 2939109-2937355 (Counterclockwise)

Preceding gene: 73542366

Following gene: 73542357

Centisome position: 77.21

GC content: 67.64

Gene sequence:

>1755_bases
ATGCATACTGAAAAGAGCGACTATGCCGTCAGCCAGCGCCTGTTGCTGCTGTGCGGCATCGCGTTGCTGATTGGCGTCGT
CAGCACGCTGGCCGCTGACGTGCTGCTGAAGCTGATCCGCTTCTTCACCAACCTGTTCTTCTACCAGACGCTGTCGCTCG
CGGAACGTTCGCCGGCGCACCACACGCTCGGCGCGTGGGTCATCGTCGTGCCCGTGCTGGGCGGATTGATCGTCGGGCTG
ATGGCCCGCTATGGTAGCGACAAGATCCGCGGCCACGGCATTCCCGAAGCCATCGAAGCTGTGCTGTTCGGGAAGAGCAA
GATGTCGCCACGCGTGGCCGTGCTCAAGCCGCTGTCGTCCGGCATCGTAATCGGCAGCGGCGGGCCGTTCGGCGCGGAAG
GCCCGATTATCATGACCGGCGGTTCGCTGGCGTCATTGCTCGCGCAGTACCTGCATCTGACTGCTGGCGAGCGCAAGTCG
CTGCTGGTGGCGGGCGCTTGCGCGGGTATGACGGCGATCTTCGGCACACCCGTGGCAGCCGTGCTGCTCGCCATCGAGCT
TTTGCTGTTTGAACTGCGCCCGCGCAGCCTGCTGCCGGTAGCGCTGGCCTGCGCGGTGGCGGGTTTCCTGCGGCCGTTCT
TCTTTGAAGCGGGTCCGCTGTTCCCGTTGCAAACGGCGCCGGCCGGCCCCGTAGCGCTCGGCTCGTGCGTGGTGGCAGGG
CTGTTGTGCGGTGCGCTGGGCGCTGCGCTGACGCTTGCGCTGTACCGCACGGAAGACGCCTTCTCGCGGTTGCCGCTGCA
CTGGATGTGGTGGCCTGCCATCGGCGGCCTGCTGGTTGGCATCGGCGGCTATTTCGAGCCGCGCGCGCTTGGCGTTGGCT
ATGACGTGATCGGCGACCTGCTGAACAATCATCTCGCGATCAGTGTCGCGGTGGCCTTGCTGGTGGTGAAGGCGCTGATC
TGGATCGCAGCGCTCGGTTCCGGCACGTCGGGCGGCGTGCTCGCGCCGCTGCTGATGCTCGGCGCGGGGCTTGGCACCGT
GCTGGCCCCATGGCTGCCAGGCGGCTCGCCGCAACTGTGGGCGCTGGTCTGCATGGCCGGCGTGCTTGGCAGCGTGCTCG
GCGCGCCGCTCACGGCCATCGTGTTCGCGTTCGGGCTCACGCACAATACCGAGGCGCTGCTGCCGCTGCTGCTGACCACC
GCGGTGGCGTACGGCTTCTCGGTGCTGACTATGAAGCGTTCGATCATGACGGAGAAGATCGCGCGGCGCGGCCTGCATAT
CTATCGCGAATATAGCGTCGATCCGCTCGAACGTGCCCATGTGGCGGAGCTGATGACGCGCGATGTCATCACGATCGACG
CCGACCTCGGCGTGTCCGAGGCGACCGCGCGCTACTTCGGTGAACACGCCGCGCACCGCGCCTACCCCGTGGTATCGGGC
GACCGCGTGCTCGGCATGCTGGATCGTGCCACGGTGCGTGGCGCCGCGGCCGGCTCGGCGCCGGCTAACCTGCGCTGCCG
CGACCTGCTCGTGGCACAAGCGCAAGTCCTGATGCCGGCGCAGACTGCGCGCGCGGCCGCAGGCAGCATGGCCGCGTCGA
GCGTGGCGCGGCTGCCGGTGGTGGAGAGCCTCGACACCATGCGGCTGGTCGGCATAGTCTCGCTGCGCGACCTGCTGCAG
CCGAGCGGCCAGGTCTTCCACGAGGAATTCCGGCGGGAGCGCCTGCGCGGCTCGCAACAACAACTCGCAAGCTAA

Upstream 100 bases:

>100_bases
ATCACGGAGTGATATATTTGGCGGCCTCTGGCAGAGTCATTGTCGTACGGACGTTCCGCGCTGATTCTTGCCGCATCGCA
CAAGAATCCAAGGAGTCCCC

Downstream 100 bases:

>100_bases
GCGTCTTCGCAACCGCTACAGCTTGTAAGTCGACAGCAGGATGCTGGTCTCGGTGGCGCTGATCCCGTCGATCAGCCGGA
TCCGGTCCAGCGTGCGGTCG

Product: CBS:Cl- channel, voltage gated

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MHTEKSDYAVSQRLLLLCGIALLIGVVSTLAADVLLKLIRFFTNLFFYQTLSLAERSPAHHTLGAWVIVVPVLGGLIVGL
MARYGSDKIRGHGIPEAIEAVLFGKSKMSPRVAVLKPLSSGIVIGSGGPFGAEGPIIMTGGSLASLLAQYLHLTAGERKS
LLVAGACAGMTAIFGTPVAAVLLAIELLLFELRPRSLLPVALACAVAGFLRPFFFEAGPLFPLQTAPAGPVALGSCVVAG
LLCGALGAALTLALYRTEDAFSRLPLHWMWWPAIGGLLVGIGGYFEPRALGVGYDVIGDLLNNHLAISVAVALLVVKALI
WIAALGSGTSGGVLAPLLMLGAGLGTVLAPWLPGGSPQLWALVCMAGVLGSVLGAPLTAIVFAFGLTHNTEALLPLLLTT
AVAYGFSVLTMKRSIMTEKIARRGLHIYREYSVDPLERAHVAELMTRDVITIDADLGVSEATARYFGEHAAHRAYPVVSG
DRVLGMLDRATVRGAAAGSAPANLRCRDLLVAQAQVLMPAQTARAAAGSMAASSVARLPVVESLDTMRLVGIVSLRDLLQ
PSGQVFHEEFRRERLRGSQQQLAS

Sequences:

>Translated_584_residues
MHTEKSDYAVSQRLLLLCGIALLIGVVSTLAADVLLKLIRFFTNLFFYQTLSLAERSPAHHTLGAWVIVVPVLGGLIVGL
MARYGSDKIRGHGIPEAIEAVLFGKSKMSPRVAVLKPLSSGIVIGSGGPFGAEGPIIMTGGSLASLLAQYLHLTAGERKS
LLVAGACAGMTAIFGTPVAAVLLAIELLLFELRPRSLLPVALACAVAGFLRPFFFEAGPLFPLQTAPAGPVALGSCVVAG
LLCGALGAALTLALYRTEDAFSRLPLHWMWWPAIGGLLVGIGGYFEPRALGVGYDVIGDLLNNHLAISVAVALLVVKALI
WIAALGSGTSGGVLAPLLMLGAGLGTVLAPWLPGGSPQLWALVCMAGVLGSVLGAPLTAIVFAFGLTHNTEALLPLLLTT
AVAYGFSVLTMKRSIMTEKIARRGLHIYREYSVDPLERAHVAELMTRDVITIDADLGVSEATARYFGEHAAHRAYPVVSG
DRVLGMLDRATVRGAAAGSAPANLRCRDLLVAQAQVLMPAQTARAAAGSMAASSVARLPVVESLDTMRLVGIVSLRDLLQ
PSGQVFHEEFRRERLRGSQQQLAS
>Mature_584_residues
MHTEKSDYAVSQRLLLLCGIALLIGVVSTLAADVLLKLIRFFTNLFFYQTLSLAERSPAHHTLGAWVIVVPVLGGLIVGL
MARYGSDKIRGHGIPEAIEAVLFGKSKMSPRVAVLKPLSSGIVIGSGGPFGAEGPIIMTGGSLASLLAQYLHLTAGERKS
LLVAGACAGMTAIFGTPVAAVLLAIELLLFELRPRSLLPVALACAVAGFLRPFFFEAGPLFPLQTAPAGPVALGSCVVAG
LLCGALGAALTLALYRTEDAFSRLPLHWMWWPAIGGLLVGIGGYFEPRALGVGYDVIGDLLNNHLAISVAVALLVVKALI
WIAALGSGTSGGVLAPLLMLGAGLGTVLAPWLPGGSPQLWALVCMAGVLGSVLGAPLTAIVFAFGLTHNTEALLPLLLTT
AVAYGFSVLTMKRSIMTEKIARRGLHIYREYSVDPLERAHVAELMTRDVITIDADLGVSEATARYFGEHAAHRAYPVVSG
DRVLGMLDRATVRGAAAGSAPANLRCRDLLVAQAQVLMPAQTARAAAGSMAASSVARLPVVESLDTMRLVGIVSLRDLLQ
PSGQVFHEEFRRERLRGSQQQLAS

Specific function: Unknown

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081943, Length=346, Percent_Identity=29.4797687861272, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786350, Length=286, Percent_Identity=26.5734265734266, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 61272; Mature: 61272

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTEKSDYAVSQRLLLLCGIALLIGVVSTLAADVLLKLIRFFTNLFFYQTLSLAERSPAH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HTLGAWVIVVPVLGGLIVGLMARYGSDKIRGHGIPEAIEAVLFGKSKMSPRVAVLKPLSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHEEECCCC
GIVIGSGGPFGAEGPIIMTGGSLASLLAQYLHLTAGERKSLLVAGACAGMTAIFGTPVAA
CEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCHHHH
VLLAIELLLFELRPRSLLPVALACAVAGFLRPFFFEAGPLFPLQTAPAGPVALGSCVVAG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LLCGALGAALTLALYRTEDAFSRLPLHWMWWPAIGGLLVGIGGYFEPRALGVGYDVIGDL
HHHHHHHHHHHHHHHHCHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
LNNHLAISVAVALLVVKALIWIAALGSGTSGGVLAPLLMLGAGLGTVLAPWLPGGSPQLW
HHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCHHHH
ALVCMAGVLGSVLGAPLTAIVFAFGLTHNTEALLPLLLTTAVAYGFSVLTMKRSIMTEKI
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRGLHIYREYSVDPLERAHVAELMTRDVITIDADLGVSEATARYFGEHAAHRAYPVVSG
HHCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC
DRVLGMLDRATVRGAAAGSAPANLRCRDLLVAQAQVLMPAQTARAAAGSMAASSVARLPV
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHH
VESLDTMRLVGIVSLRDLLQPSGQVFHEEFRRERLRGSQQQLAS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MHTEKSDYAVSQRLLLLCGIALLIGVVSTLAADVLLKLIRFFTNLFFYQTLSLAERSPAH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HTLGAWVIVVPVLGGLIVGLMARYGSDKIRGHGIPEAIEAVLFGKSKMSPRVAVLKPLSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHEEECCCC
GIVIGSGGPFGAEGPIIMTGGSLASLLAQYLHLTAGERKSLLVAGACAGMTAIFGTPVAA
CEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCHHHH
VLLAIELLLFELRPRSLLPVALACAVAGFLRPFFFEAGPLFPLQTAPAGPVALGSCVVAG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LLCGALGAALTLALYRTEDAFSRLPLHWMWWPAIGGLLVGIGGYFEPRALGVGYDVIGDL
HHHHHHHHHHHHHHHHCHHHHHHCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
LNNHLAISVAVALLVVKALIWIAALGSGTSGGVLAPLLMLGAGLGTVLAPWLPGGSPQLW
HHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCHHHH
ALVCMAGVLGSVLGAPLTAIVFAFGLTHNTEALLPLLLTTAVAYGFSVLTMKRSIMTEKI
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRGLHIYREYSVDPLERAHVAELMTRDVITIDADLGVSEATARYFGEHAAHRAYPVVSG
HHCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC
DRVLGMLDRATVRGAAAGSAPANLRCRDLLVAQAQVLMPAQTARAAAGSMAASSVARLPV
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHH
VESLDTMRLVGIVSLRDLLQPSGQVFHEEFRRERLRGSQQQLAS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]