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The map label for this gene is pilJ [H]

Identifier: 73542305

GI number: 73542305

Start: 2876123

End: 2878324

Strand: Reverse

Name: pilJ [H]

Synonym: Reut_A2620

Alternate gene names: 73542305

Gene position: 2878324-2876123 (Counterclockwise)

Preceding gene: 73542306

Following gene: 73542304

Centisome position: 75.62

GC content: 66.62

Gene sequence:

>2202_bases
ATGGGGTTCAAGAAAATCAGCCTGGGACGCCGGAAGCCGGCAGCCGACGCGGCCGCCGGCCTGGTGGGAGACCCCGCCGG
CGCGGCACCATCGGCGAGTACCGCACCGCGTGCGGACGGGCTGGGCAGAACTCCGCTGGAGGCCGTGTCGGCATTGCTGT
CGCGCATGCCGTTCTCCTCGCAGCAGCGTGCGCTGACCATCGGCGTGGTGGTGTCGTTGGTCGCGCTGCTCGGCTCGGTG
TATATGGATAACCGCATCGCCAACAATGCCGCGGCGCAGATCGAAATCTCAGGCGACATGCTGATGCACTCGCAGCGCCT
GGGTAAGGCCGCGCCAGTGGCGCTGCTGGGCAATATGCAGGCCTTCACGCAGCTGCGCCAGTCCAAGGAAGCGCTGGCCG
CTGACTTGCAGGCGCTGCAGGCCGGCAGCGATGACAGGCACGTGCGCGCCACGACGGGCGAGGCACAGCCGCTGCTCGAA
ACCGCGATCAGCTCGTGGAAGCGCAGCGAGAAGAGCGCCGCCGACGTGCTCGCGCAGCAGGCGGTGCTGACGACGATCGG
CCAGACGCTGCAGGTTTTCAACGCATCCAACCCCGAACTGCTCGAAGCCGCCGAACAGGTGGCGGCGCTCAAGCTGCAAT
CAGGCTCCAATGCGCGTGAGGTGGCCGCATCCGCCCAGCTCGTGATGCTGACGCAGCGCTTGGGCAAGAACCTGAATGAG
TTCCTGTCCGGCGAAGGCGTGAACCCCGAAACCGCGTTCCTGCTCGGCAAGGACACCAACACCTTCCGCGAGACGCTGGA
CGGTCTGCTCAACGGCAGCGAGGCGCTGCGGCTGAATGCCGCCACCGATGCCGAGACGCGCGGGTACCTGCAGCAGCTGT
CGCAGCGCTTCGACGCGGTGCAGAAGACCACCCAGACGATCCTGCAGAACCTGCCCGGCCTGATTGCCGCCAAGCGCGCG
CAGCAGCAGATCTTCAACGACAACGAAAGCCTGCGCGGCGAGCTGTCGGCGCTGCAGAAGTCTTACTCCGAAGGCGCGCG
CTTCCGTCCGGTGACGCTGGGCGCGATGCTGGTTTCAGCGCTGGCCACGCTGCTCTGCCTGGCCGGCCTGGCCTCGCTGT
ACCTGCGCGATTCGCGCCTGCGCGCGGTCGAAGCCGAAGCGCGCGAACGTGAAGCCGAAGCACGCCGCCTGGAAGAAAAA
CGCAACAACGACACCACGCAAAAGGCCATTCTGCAGCTGATGAACGAACTGCAGGACATCGCCGACGGTGACCTGACACG
CCAGGCGACCGTGACCGAGGACATCACCGGCGCCATTGCCGACTCGGTGAACTATACGGTGGAAGAACTGCGCGAACTGG
TGGGCCGCGTGCAGCAGACGGCAGGGGAAGTGACGCTGGCGTCGGGACAGGTGCAGGCCACGTCCAACCAGCTTGCCACG
ACGACCGAAGAGCAGTCGCGCCAGATCCGCCAGACCGGTGAGTCCGTGGTGGACATGGCGGATCGCATCACGCAGGTGTC
GCGCGGTGCGGCGGAGTCTGCCAACGTGGCGCGCGCCTCGCTGACGGCTGCGGAGCAAGGGCAGCAGGCGGTGCAGAACG
CGATCGCGGGCATGAACGACATCCGCGAGCAGATCCAGGAAACGTCCAAGCGCATCAAGCGGCTGGGCGAGTCGTCGCAG
GAGATCGGCGAAATCGTCGAGCTGATCTCGGACATTACCGAGCAGACCAACGTGCTGGCACTCAACGCCGCCATCCAGGC
CGCATCGGCTGGTGAGGCCGGCCGCGGCTTCACGGTGGTGGCCGAAGAAGTGCAGCGCCTGGCGGAACGCTCGGGGGAAG
CAACCAAGCAGATTGGCGCGCTGATCCGCACCATCCAGACCGACACGCAGGATGCCGTGCACGCGATGGAGCGCAGCACG
CAGGGTGTGGTCGAAGGGGCGCGCCTGTCGGACAATGCCGGCGCCGCACTGCAGGAAATCGGCCGCGTGTCGCGCCAGCT
TGCCGAACTGATCGAACAGATCTCGCGCTCCACTTCGCACGAAGCGGATCTCGCTGGCGGCGTGGCCCGCCACATCGAGC
GCATTCTGCAGGCGACCGAGCAGACCACCGCCGGTACGCGCCAGACGGCGAATTCGGTGCGCGATCTGACGCAGCTCACC
GAAGAGCTGCGCAACTCCGTGTCGCGGTTCAAGATCGCCTGA

Upstream 100 bases:

>100_bases
TCCGGCGCCGTTGGTCGCGGTGCCCGTGGCAACAGAACAAATAGCGCGGCGGCCCGTGTCGAGGCTGCCCCAGATAACGA
TTCAACGGACAGAGGGTGCT

Downstream 100 bases:

>100_bases
CCATGAACAGGCACGGGGTTTCCCGCCAGCATGCCGGTATCGCGCATGCCGGCTGCAAGGCAGCCCCGGTGCGAATGACG
ATCCTGGCGCCCGCCGCGTC

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MGFKKISLGRRKPAADAAAGLVGDPAGAAPSASTAPRADGLGRTPLEAVSALLSRMPFSSQQRALTIGVVVSLVALLGSV
YMDNRIANNAAAQIEISGDMLMHSQRLGKAAPVALLGNMQAFTQLRQSKEALAADLQALQAGSDDRHVRATTGEAQPLLE
TAISSWKRSEKSAADVLAQQAVLTTIGQTLQVFNASNPELLEAAEQVAALKLQSGSNAREVAASAQLVMLTQRLGKNLNE
FLSGEGVNPETAFLLGKDTNTFRETLDGLLNGSEALRLNAATDAETRGYLQQLSQRFDAVQKTTQTILQNLPGLIAAKRA
QQQIFNDNESLRGELSALQKSYSEGARFRPVTLGAMLVSALATLLCLAGLASLYLRDSRLRAVEAEAREREAEARRLEEK
RNNDTTQKAILQLMNELQDIADGDLTRQATVTEDITGAIADSVNYTVEELRELVGRVQQTAGEVTLASGQVQATSNQLAT
TTEEQSRQIRQTGESVVDMADRITQVSRGAAESANVARASLTAAEQGQQAVQNAIAGMNDIREQIQETSKRIKRLGESSQ
EIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMERST
QGVVEGARLSDNAGAALQEIGRVSRQLAELIEQISRSTSHEADLAGGVARHIERILQATEQTTAGTRQTANSVRDLTQLT
EELRNSVSRFKIA

Sequences:

>Translated_733_residues
MGFKKISLGRRKPAADAAAGLVGDPAGAAPSASTAPRADGLGRTPLEAVSALLSRMPFSSQQRALTIGVVVSLVALLGSV
YMDNRIANNAAAQIEISGDMLMHSQRLGKAAPVALLGNMQAFTQLRQSKEALAADLQALQAGSDDRHVRATTGEAQPLLE
TAISSWKRSEKSAADVLAQQAVLTTIGQTLQVFNASNPELLEAAEQVAALKLQSGSNAREVAASAQLVMLTQRLGKNLNE
FLSGEGVNPETAFLLGKDTNTFRETLDGLLNGSEALRLNAATDAETRGYLQQLSQRFDAVQKTTQTILQNLPGLIAAKRA
QQQIFNDNESLRGELSALQKSYSEGARFRPVTLGAMLVSALATLLCLAGLASLYLRDSRLRAVEAEAREREAEARRLEEK
RNNDTTQKAILQLMNELQDIADGDLTRQATVTEDITGAIADSVNYTVEELRELVGRVQQTAGEVTLASGQVQATSNQLAT
TTEEQSRQIRQTGESVVDMADRITQVSRGAAESANVARASLTAAEQGQQAVQNAIAGMNDIREQIQETSKRIKRLGESSQ
EIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMERST
QGVVEGARLSDNAGAALQEIGRVSRQLAELIEQISRSTSHEADLAGGVARHIERILQATEQTTAGTRQTANSVRDLTQLT
EELRNSVSRFKIA
>Mature_732_residues
GFKKISLGRRKPAADAAAGLVGDPAGAAPSASTAPRADGLGRTPLEAVSALLSRMPFSSQQRALTIGVVVSLVALLGSVY
MDNRIANNAAAQIEISGDMLMHSQRLGKAAPVALLGNMQAFTQLRQSKEALAADLQALQAGSDDRHVRATTGEAQPLLET
AISSWKRSEKSAADVLAQQAVLTTIGQTLQVFNASNPELLEAAEQVAALKLQSGSNAREVAASAQLVMLTQRLGKNLNEF
LSGEGVNPETAFLLGKDTNTFRETLDGLLNGSEALRLNAATDAETRGYLQQLSQRFDAVQKTTQTILQNLPGLIAAKRAQ
QQIFNDNESLRGELSALQKSYSEGARFRPVTLGAMLVSALATLLCLAGLASLYLRDSRLRAVEAEAREREAEARRLEEKR
NNDTTQKAILQLMNELQDIADGDLTRQATVTEDITGAIADSVNYTVEELRELVGRVQQTAGEVTLASGQVQATSNQLATT
TEEQSRQIRQTGESVVDMADRITQVSRGAAESANVARASLTAAEQGQQAVQNAIAGMNDIREQIQETSKRIKRLGESSQE
IGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMERSTQ
GVVEGARLSDNAGAALQEIGRVSRQLAELIEQISRSTSHEADLAGGVARHIERILQATEQTTAGTRQTANSVRDLTQLTE
ELRNSVSRFKIA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788194, Length=326, Percent_Identity=30.9815950920245, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI2367378, Length=290, Percent_Identity=29.3103448275862, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788195, Length=323, Percent_Identity=26.625386996904, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789453, Length=270, Percent_Identity=31.4814814814815, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787690, Length=282, Percent_Identity=31.5602836879433, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 78301; Mature: 78170

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKKISLGRRKPAADAAAGLVGDPAGAAPSASTAPRADGLGRTPLEAVSALLSRMPFSS
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QQRALTIGVVVSLVALLGSVYMDNRIANNAAAQIEISGDMLMHSQRLGKAAPVALLGNMQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCHHHHCCHH
AFTQLRQSKEALAADLQALQAGSDDRHVRATTGEAQPLLETAISSWKRSEKSAADVLAQQ
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLTTIGQTLQVFNASNPELLEAAEQVAALKLQSGSNAREVAASAQLVMLTQRLGKNLNE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
FLSGEGVNPETAFLLGKDTNTFRETLDGLLNGSEALRLNAATDAETRGYLQQLSQRFDAV
HHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHH
QKTTQTILQNLPGLIAAKRAQQQIFNDNESLRGELSALQKSYSEGARFRPVTLGAMLVSA
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LATLLCLAGLASLYLRDSRLRAVEAEAREREAEARRLEEKRNNDTTQKAILQLMNELQDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ADGDLTRQATVTEDITGAIADSVNYTVEELRELVGRVQQTAGEVTLASGQVQATSNQLAT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCHHHC
TTEEQSRQIRQTGESVVDMADRITQVSRGAAESANVARASLTAAEQGQQAVQNAIAGMND
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
IREQIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFTVV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHCCCCCCCCHHHH
AEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMERSTQGVVEGARLSDNAGAALQEI
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
GRVSRQLAELIEQISRSTSHEADLAGGVARHIERILQATEQTTAGTRQTANSVRDLTQLT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EELRNSVSRFKIA
HHHHHHHHHHCCC
>Mature Secondary Structure 
GFKKISLGRRKPAADAAAGLVGDPAGAAPSASTAPRADGLGRTPLEAVSALLSRMPFSS
CCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QQRALTIGVVVSLVALLGSVYMDNRIANNAAAQIEISGDMLMHSQRLGKAAPVALLGNMQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCHHHHCCHH
AFTQLRQSKEALAADLQALQAGSDDRHVRATTGEAQPLLETAISSWKRSEKSAADVLAQQ
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLTTIGQTLQVFNASNPELLEAAEQVAALKLQSGSNAREVAASAQLVMLTQRLGKNLNE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
FLSGEGVNPETAFLLGKDTNTFRETLDGLLNGSEALRLNAATDAETRGYLQQLSQRFDAV
HHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHH
QKTTQTILQNLPGLIAAKRAQQQIFNDNESLRGELSALQKSYSEGARFRPVTLGAMLVSA
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LATLLCLAGLASLYLRDSRLRAVEAEAREREAEARRLEEKRNNDTTQKAILQLMNELQDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ADGDLTRQATVTEDITGAIADSVNYTVEELRELVGRVQQTAGEVTLASGQVQATSNQLAT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCHHHC
TTEEQSRQIRQTGESVVDMADRITQVSRGAAESANVARASLTAAEQGQQAVQNAIAGMND
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
IREQIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFTVV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHCCCCCCCCHHHH
AEEVQRLAERSGEATKQIGALIRTIQTDTQDAVHAMERSTQGVVEGARLSDNAGAALQEI
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
GRVSRQLAELIEQISRSTSHEADLAGGVARHIERILQATEQTTAGTRQTANSVRDLTQLT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EELRNSVSRFKIA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]