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The map label for this gene is dadA2 [H]

Identifier: 73542274

GI number: 73542274

Start: 2839625

End: 2840905

Strand: Reverse

Name: dadA2 [H]

Synonym: Reut_A2589

Alternate gene names: 73542274

Gene position: 2840905-2839625 (Counterclockwise)

Preceding gene: 73542277

Following gene: 73542273

Centisome position: 74.63

GC content: 67.84

Gene sequence:

>1281_bases
ATGCGGGTAGTGATCGTAGGCGCCGGCGTGGTCGGCATGACCAGTGCGTGGCGCCTGGCGCAGGATGGCCACGAGGTGAC
GGTGCTCGAACGCCATGACGGCCCTGGCGAGGAAACCAGCTTCGCCAACGGCGGGCAGCTTAGCTACAGCTACGTGGCGC
CGCTGGCCGGACCGGGCGTGATGGGCAAGGTGCCAGGCTGGCTTCTGCGGCGCGATTCGCCGATGCGCTTCCGGCCCGTA
GCCGATCCCGCGCAGTGGCTCTGGCTGGCGGCATTCATGCGCGCTTGCAATGCCGGCACCAGCGAGGCCACCACGCGCAA
GCTGCTGCGCCTGGCATTTTACTCGCGCGACCTGATGGAGGCGTTCGTCGCCAGCGAAGGCGCGAAGGCCGGGACGGCGA
GCGATTTCGATTTTGCGCGGCGCGGCAAACTGATTGTGCACCGCGATGGCGCGGCGTTTGAATCGGCGCGCCGCTTGCTC
GATTATCAAGCCAGTCTCGGGTGCGAACAGCAGGCACTCGACCGGGACGCGTGCGTGGCGCTGGAACCCGCGCTGGCAGG
TATCCGCGACGAGATCGCCGGCGCGATCTACACACCGAGCGAGGAGGTGGGCGACTGCCATCGCTTCTGCCTGTCGCTGG
CACGGTTGCTGCAGCATCAGCCGGGCGTGTCGATGCGGTTCGGCAATGTCGTGCACGGCCTGGTGCAACAAGGGGACCGC
GTCACGGGCGTGCAGACGGTAGCCGGCGTAGTGCCCGCCGATGCGGTGGTGGTGGCGGGTGGCATCGGCAGCGTGCCGCT
GCTGCGCCCACTGGGCGTGCGGCCGATGCTTTGGCCGTTGAAGGGCTACAGCATTACCGTGCCGCTGTCGAACAGTGGGC
CGGCTCCGCACATCAGCATCACGGATTTCGCCAACAAGATCGTCTATGCACGCATCGGCAATACGCTGCGCGTCGCGGGC
ATGGCCGATCTCGTGCGTGGTGGCGCGCGGATCGATCCCGAGCGCATTGGCACGCTGGTGGCGCAGACGCGCACGGTGTT
TGAGGGTATCGTGCCCGACATCCCGCTCGCGCAGTTGCAGCCGTGGGCAGGGCTGCGGCCCGCGACGCCCGATGGCTTGC
CGATGGTCGGGCCATCACGCCTGCGCGGCCTGTGGCTCAATCTCGGGCATGGCGCGCTTGGCTTTACGCTTGCTATGGGC
AGCGCAGGGCTGCTTGCCGACCGCCTTGCCGGACGCAAGCCTGCCGTCAATGGCGATGATTTCATCGCCGCGCACGCGTA
G

Upstream 100 bases:

>100_bases
TAGCCTAAAGACATATTCTGGCACGTATTGGCATTGGGCAAGCCGCGTGCACTCGACTACGCTGACGCCATTGAATGAAC
AGCAAGCGACAAGGATGGCA

Downstream 100 bases:

>100_bases
TTGCGCGGCGGAATCCTTGCAGTAGCGGGCTATACAACTGTGGTTCGTATCGCCGTTCCCGCCGCGGGGCATCAACATGA
GCGGCAAGGCGTTCCAGAGG

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRVVIVGAGVVGMTSAWRLAQDGHEVTVLERHDGPGEETSFANGGQLSYSYVAPLAGPGVMGKVPGWLLRRDSPMRFRPV
ADPAQWLWLAAFMRACNAGTSEATTRKLLRLAFYSRDLMEAFVASEGAKAGTASDFDFARRGKLIVHRDGAAFESARRLL
DYQASLGCEQQALDRDACVALEPALAGIRDEIAGAIYTPSEEVGDCHRFCLSLARLLQHQPGVSMRFGNVVHGLVQQGDR
VTGVQTVAGVVPADAVVVAGGIGSVPLLRPLGVRPMLWPLKGYSITVPLSNSGPAPHISITDFANKIVYARIGNTLRVAG
MADLVRGGARIDPERIGTLVAQTRTVFEGIVPDIPLAQLQPWAGLRPATPDGLPMVGPSRLRGLWLNLGHGALGFTLAMG
SAGLLADRLAGRKPAVNGDDFIAAHA

Sequences:

>Translated_426_residues
MRVVIVGAGVVGMTSAWRLAQDGHEVTVLERHDGPGEETSFANGGQLSYSYVAPLAGPGVMGKVPGWLLRRDSPMRFRPV
ADPAQWLWLAAFMRACNAGTSEATTRKLLRLAFYSRDLMEAFVASEGAKAGTASDFDFARRGKLIVHRDGAAFESARRLL
DYQASLGCEQQALDRDACVALEPALAGIRDEIAGAIYTPSEEVGDCHRFCLSLARLLQHQPGVSMRFGNVVHGLVQQGDR
VTGVQTVAGVVPADAVVVAGGIGSVPLLRPLGVRPMLWPLKGYSITVPLSNSGPAPHISITDFANKIVYARIGNTLRVAG
MADLVRGGARIDPERIGTLVAQTRTVFEGIVPDIPLAQLQPWAGLRPATPDGLPMVGPSRLRGLWLNLGHGALGFTLAMG
SAGLLADRLAGRKPAVNGDDFIAAHA
>Mature_426_residues
MRVVIVGAGVVGMTSAWRLAQDGHEVTVLERHDGPGEETSFANGGQLSYSYVAPLAGPGVMGKVPGWLLRRDSPMRFRPV
ADPAQWLWLAAFMRACNAGTSEATTRKLLRLAFYSRDLMEAFVASEGAKAGTASDFDFARRGKLIVHRDGAAFESARRLL
DYQASLGCEQQALDRDACVALEPALAGIRDEIAGAIYTPSEEVGDCHRFCLSLARLLQHQPGVSMRFGNVVHGLVQQGDR
VTGVQTVAGVVPADAVVVAGGIGSVPLLRPLGVRPMLWPLKGYSITVPLSNSGPAPHISITDFANKIVYARIGNTLRVAG
MADLVRGGARIDPERIGTLVAQTRTVFEGIVPDIPLAQLQPWAGLRPATPDGLPMVGPSRLRGLWLNLGHGALGFTLAMG
SAGLLADRLAGRKPAVNGDDFIAAHA

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=429, Percent_Identity=38.4615384615385, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 45188; Mature: 45188

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVIVGAGVVGMTSAWRLAQDGHEVTVLERHDGPGEETSFANGGQLSYSYVAPLAGPGV
CEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
MGKVPGWLLRRDSPMRFRPVADPAQWLWLAAFMRACNAGTSEATTRKLLRLAFYSRDLME
CCCCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AFVASEGAKAGTASDFDFARRGKLIVHRDGAAFESARRLLDYQASLGCEQQALDRDACVA
HHHHCCCCCCCCCCCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHCCCCCCEE
LEPALAGIRDEIAGAIYTPSEEVGDCHRFCLSLARLLQHQPGVSMRFGNVVHGLVQQGDR
ECHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCC
VTGVQTVAGVVPADAVVVAGGIGSVPLLRPLGVRPMLWPLKGYSITVPLSNSGPAPHISI
CCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEH
TDFANKIVYARIGNTLRVAGMADLVRGGARIDPERIGTLVAQTRTVFEGIVPDIPLAQLQ
HHHHHHEEEEECCCEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCC
PWAGLRPATPDGLPMVGPSRLRGLWLNLGHGALGFTLAMGSAGLLADRLAGRKPAVNGDD
CCCCCCCCCCCCCCCCCHHHHCEEEEECCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCC
FIAAHA
EEEECC
>Mature Secondary Structure
MRVVIVGAGVVGMTSAWRLAQDGHEVTVLERHDGPGEETSFANGGQLSYSYVAPLAGPGV
CEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
MGKVPGWLLRRDSPMRFRPVADPAQWLWLAAFMRACNAGTSEATTRKLLRLAFYSRDLME
CCCCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AFVASEGAKAGTASDFDFARRGKLIVHRDGAAFESARRLLDYQASLGCEQQALDRDACVA
HHHHCCCCCCCCCCCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHCCCCCCEE
LEPALAGIRDEIAGAIYTPSEEVGDCHRFCLSLARLLQHQPGVSMRFGNVVHGLVQQGDR
ECHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCC
VTGVQTVAGVVPADAVVVAGGIGSVPLLRPLGVRPMLWPLKGYSITVPLSNSGPAPHISI
CCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCEEEH
TDFANKIVYARIGNTLRVAGMADLVRGGARIDPERIGTLVAQTRTVFEGIVPDIPLAQLQ
HHHHHHEEEEECCCEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCC
PWAGLRPATPDGLPMVGPSRLRGLWLNLGHGALGFTLAMGSAGLLADRLAGRKPAVNGDD
CCCCCCCCCCCCCCCCCHHHHCEEEEECCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCC
FIAAHA
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]