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The map label for this gene is gmk

Identifier: 73542159

GI number: 73542159

Start: 2713555

End: 2714205

Strand: Reverse

Name: gmk

Synonym: Reut_A2473

Alternate gene names: 73542159

Gene position: 2714205-2713555 (Counterclockwise)

Preceding gene: 73542160

Following gene: 73542158

Centisome position: 71.3

GC content: 64.52

Gene sequence:

>651_bases
ATGAGCGATACGCCTCACACCGCCATCGATACCGTCTACCCCGGCAACCTGTTCATGGTGGTGGCACCTTCCGGAGCCGG
CAAGTCGACGCTGGTCAACGCGCTGCTCGCGCAGGACGCGGCGATCCGCCTGTCGATCTCCCATACCACGCGTGCGCCGC
GCCCCGGCGAACAGGATGGGCGCGAGTATCACTTCTGCACAGTGGACTCCTTCCGCGCTGCGCGCGACCGCGGCGAGTTC
CTCGAGTGGGCCGAGGTCCACGGCAATTACTACGCGACGTCGCGCGTGTGGATCGAGGAGCAGATGGCGCAGGGCAATGA
CGTGCTGCTCGAGATCGACTGGCAGGGCGCGCAACAGGTGCACCAGCGGTTCTCGAACGCGGTCGAGATCTTCATCCTGC
CGCCGTCGCTGACTGCGCTGGAAGACCGCCTGAAGAAGCGTGGCCAGGACGAGCCCAACGTGATCGTGCGGCGCCTGCTG
GCGGCTGGCAGCGAGATGTCGCATGCGTCCGAATCGGACTACGTCATCATCAACGAGGTCTTCGACAACGCGCTCGAGGA
ACTGCGCAACGTGGTGCGTGCCACGCGCCTGCGCTTTTCGTCGCAAAAAGCGCGCCATACCGAGCTCTTCATCGAGCTTG
GCATTCACTGA

Upstream 100 bases:

>100_bases
TCGAGCAGATGCGCGAGCAGGTCCAGAACCTCGAGTAACGCCCCGTTCAACGCATCCGTTTCACCTGGCGCTACGGCGCC
TGAAGAACCAAAGAATTCAC

Downstream 100 bases:

>100_bases
GCGCCAGTGCGCGCGGGGGCCCTGCCGGGCGATTCGCGCGCGGTGCTGTAAAATAGCAACCCAACAGGTTTTGTCCCAAG
GTGGATTTGATATGGCGCGT

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSDTPHTAIDTVYPGNLFMVVAPSGAGKSTLVNALLAQDAAIRLSISHTTRAPRPGEQDGREYHFCTVDSFRAARDRGEF
LEWAEVHGNYYATSRVWIEEQMAQGNDVLLEIDWQGAQQVHQRFSNAVEIFILPPSLTALEDRLKKRGQDEPNVIVRRLL
AAGSEMSHASESDYVIINEVFDNALEELRNVVRATRLRFSSQKARHTELFIELGIH

Sequences:

>Translated_216_residues
MSDTPHTAIDTVYPGNLFMVVAPSGAGKSTLVNALLAQDAAIRLSISHTTRAPRPGEQDGREYHFCTVDSFRAARDRGEF
LEWAEVHGNYYATSRVWIEEQMAQGNDVLLEIDWQGAQQVHQRFSNAVEIFILPPSLTALEDRLKKRGQDEPNVIVRRLL
AAGSEMSHASESDYVIINEVFDNALEELRNVVRATRLRFSSQKARHTELFIELGIH
>Mature_215_residues
SDTPHTAIDTVYPGNLFMVVAPSGAGKSTLVNALLAQDAAIRLSISHTTRAPRPGEQDGREYHFCTVDSFRAARDRGEFL
EWAEVHGNYYATSRVWIEEQMAQGNDVLLEIDWQGAQQVHQRFSNAVEIFILPPSLTALEDRLKKRGQDEPNVIVRRLLA
AGSEMSHASESDYVIINEVFDNALEELRNVVRATRLRFSSQKARHTELFIELGIH

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874833, Length=178, Percent_Identity=38.7640449438202, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI4504221, Length=178, Percent_Identity=38.7640449438202, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI226874831, Length=178, Percent_Identity=38.7640449438202, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI38570142, Length=178, Percent_Identity=27.5280898876405, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI4505237, Length=173, Percent_Identity=26.5895953757225, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI262118259, Length=173, Percent_Identity=26.5895953757225, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI262118255, Length=173, Percent_Identity=25.4335260115607, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI262118263, Length=173, Percent_Identity=26.5895953757225, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1790080, Length=201, Percent_Identity=52.7363184079602, Blast_Score=211, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI193203132, Length=185, Percent_Identity=32.4324324324324, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17508835, Length=185, Percent_Identity=32.4324324324324, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320662, Length=182, Percent_Identity=37.9120879120879, Blast_Score=132, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21356141, Length=179, Percent_Identity=40.7821229050279, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_CUPPJ (Q46YE8)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296679.1
- HSSP:   P60546
- ProteinModelPortal:   Q46YE8
- SMR:   Q46YE8
- GeneID:   3612196
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2473
- NMPDR:   fig|264198.3.peg.3244
- HOGENOM:   HBG671982
- OMA:   PPTWEEL
- ProtClustDB:   PRK00300
- BioCyc:   REUT264198:REUT_A2473-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 24318; Mature: 24186

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPHTAIDTVYPGNLFMVVAPSGAGKSTLVNALLAQDAAIRLSISHTTRAPRPGEQDG
CCCCCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCC
REYHFCTVDSFRAARDRGEFLEWAEVHGNYYATSRVWIEEQMAQGNDVLLEIDWQGAQQV
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEHEEHHHHHHCCCCCEEEEEECCCHHHH
HQRFSNAVEIFILPPSLTALEDRLKKRGQDEPNVIVRRLLAAGSEMSHASESDYVIINEV
HHHHCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEHHH
FDNALEELRNVVRATRLRFSSQKARHTELFIELGIH
HHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCC
>Mature Secondary Structure 
SDTPHTAIDTVYPGNLFMVVAPSGAGKSTLVNALLAQDAAIRLSISHTTRAPRPGEQDG
CCCCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCC
REYHFCTVDSFRAARDRGEFLEWAEVHGNYYATSRVWIEEQMAQGNDVLLEIDWQGAQQV
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEHEEHHHHHHCCCCCEEEEEECCCHHHH
HQRFSNAVEIFILPPSLTALEDRLKKRGQDEPNVIVRRLLAAGSEMSHASESDYVIINEV
HHHHCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEHHH
FDNALEELRNVVRATRLRFSSQKARHTELFIELGIH
HHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA