Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 73542021

GI number: 73542021

Start: 2575586

End: 2577181

Strand: Direct

Name: gsiB [H]

Synonym: Reut_A2335

Alternate gene names: 73542021

Gene position: 2575586-2577181 (Clockwise)

Preceding gene: 73542019

Following gene: 73542025

Centisome position: 67.66

GC content: 64.54

Gene sequence:

>1596_bases
ATGAATTCCCGCCGCCCGTCCATCGTTTCCGGAGTCGCCCGCACCGCACTGCTGTGCGGCGCCTTGTTCGCGTTGTCGCC
GGTTGGCAGCGCATACGCCGAAGACCTGCGCGTCGGCCTGGCGGCCGATGTCACCTCCATGGACCCGCACTGGAACAATG
CCGGGCCGAACAATGCCATCGCGCTGCATATCTTCGAGTCGCTCGTGTTCATGGACAAGAATGCACGCTATATCCCGGGG
CTCGCGCTGTCGTGGAAGCCCGTCAACCCGACCACGTGGGAAATCAAGCTGCGGCCGAACGTCAAATGGCACGACGGCTC
GCCGTTTACGAGCGCGGACGTCAAGGCATCGCTCGAGCGGCCCGAGAAGCTGACCAACAGCCCCGGCTCGTTCACGAGCT
ACACCAAGCCGATCGCGCGCATCGACACGCCCGACGCGCTCACGGTGCGGCTGACCATGAACGTGGCCAACTATGCCAAC
CTGGCCAATGACCTGAACAGCGTGCCGATCATGCCGAAGAAGGTCGCCGCGACGCTTACGCAGGCCGATTTCGATTCCGG
CAAGGCCATGATCGGCACTGGCCCGTTCAAGTTCGTGCGCTTCGCCCGCGGCCAGGAAATTGTGATGGCGCGCAATCCCG
ACTACTGGGGCGCCAAGCCTGAGTGGGATCGAGCCATCTTCCGCATCATCACCGACAACGGCGCCCGCACCTCCGCCCTG
CTCGCCGGCGATGTCGACGTGATCGAAAGCGTGCCCGCCGCCGACGTGGCCAAGCTCAAGCAGAACCCGAAATTCCGCAT
CGAGCAGACCGTGTCATGGCGCACGATCTTCTGGCAGATGGACCAGTCGCGCGACAACCCGCCCTACGTCACCGACAAGG
CCGGCAAGCCGCTGGGCAAGAACCCGTTCAAGGATGCACGCGTACGCGCGGCAATCTCCAAGGCCCTCAACCGCGACGCG
ATCGTCAGCCGCATCATGGAAGGCCTTGCGGTGCCGGCCTCTTCGCTGGTGTCGCCGCAGATCTTCGGGCACCCCGGCAC
CAAACCCGACGCCTACGATCCGGAAGGCGCGAAGAAACTGCTCGCCGCCGCCGGCTATCCGAACGGTTTCGGGCTGACGC
TGCACGCAACCAACAACCGCTACCTGAACGATGCCGCCGTGGCGCAGGCTACCGCGAGCATGCTAACGCGCGTAGGCATC
CAGACCAAGGTGGAAACCTTGCCCGTGGCCGCGTACTTCACGCGCGCGCGCCAGGGCGATTTCTCGTTCGAGATGCTGGG
ATGGGGTTCCGCCGCCGCCGACGTGGCGCTGCGCTCGATCGCCGGCACGCCTGACCCCAAGACCGGCTACGGTACGTGGA
ACTGGGGCAAGTACAGCAATCCGCAGTTGGACAAGCTGATCGAGAAATCACTGACCACCGTCAGCAGCGACAAGGCGCGC
GAGGATAACGCCAAGGCCGCCGCGAAGTTCGCGCTGGACGACCATGCGGTCATCCCGTCGCATAGCCAGCTCGCGATGTG
GGCGATGAAGACCGGCCTGAAGTACGAGGCGCGCACCGACGAATGGTCGCTGGCGCAGTTCTTCCACAAGCAGTAA

Upstream 100 bases:

>100_bases
ACAATGCGGGCAAATTGCCTGACAACCGCTTGAGAACAGCCCATGGCCGCGCGCCCTCCTCGCCCGCGGCCGCCCGGGCC
AGCATGAGGAGCCTAGCGCC

Downstream 100 bases:

>100_bases
CGGCCGCGAGCCCGGAGCCTGGCTCCGGGCAGCGGACCGATCAGATCACCAGCTTTTCTTCCTGCCGCGCATTGGCGGCA
CGCAGCTTCTTCACCCAGTC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MNSRRPSIVSGVARTALLCGALFALSPVGSAYAEDLRVGLAADVTSMDPHWNNAGPNNAIALHIFESLVFMDKNARYIPG
LALSWKPVNPTTWEIKLRPNVKWHDGSPFTSADVKASLERPEKLTNSPGSFTSYTKPIARIDTPDALTVRLTMNVANYAN
LANDLNSVPIMPKKVAATLTQADFDSGKAMIGTGPFKFVRFARGQEIVMARNPDYWGAKPEWDRAIFRIITDNGARTSAL
LAGDVDVIESVPAADVAKLKQNPKFRIEQTVSWRTIFWQMDQSRDNPPYVTDKAGKPLGKNPFKDARVRAAISKALNRDA
IVSRIMEGLAVPASSLVSPQIFGHPGTKPDAYDPEGAKKLLAAAGYPNGFGLTLHATNNRYLNDAAVAQATASMLTRVGI
QTKVETLPVAAYFTRARQGDFSFEMLGWGSAAADVALRSIAGTPDPKTGYGTWNWGKYSNPQLDKLIEKSLTTVSSDKAR
EDNAKAAAKFALDDHAVIPSHSQLAMWAMKTGLKYEARTDEWSLAQFFHKQ

Sequences:

>Translated_531_residues
MNSRRPSIVSGVARTALLCGALFALSPVGSAYAEDLRVGLAADVTSMDPHWNNAGPNNAIALHIFESLVFMDKNARYIPG
LALSWKPVNPTTWEIKLRPNVKWHDGSPFTSADVKASLERPEKLTNSPGSFTSYTKPIARIDTPDALTVRLTMNVANYAN
LANDLNSVPIMPKKVAATLTQADFDSGKAMIGTGPFKFVRFARGQEIVMARNPDYWGAKPEWDRAIFRIITDNGARTSAL
LAGDVDVIESVPAADVAKLKQNPKFRIEQTVSWRTIFWQMDQSRDNPPYVTDKAGKPLGKNPFKDARVRAAISKALNRDA
IVSRIMEGLAVPASSLVSPQIFGHPGTKPDAYDPEGAKKLLAAAGYPNGFGLTLHATNNRYLNDAAVAQATASMLTRVGI
QTKVETLPVAAYFTRARQGDFSFEMLGWGSAAADVALRSIAGTPDPKTGYGTWNWGKYSNPQLDKLIEKSLTTVSSDKAR
EDNAKAAAKFALDDHAVIPSHSQLAMWAMKTGLKYEARTDEWSLAQFFHKQ
>Mature_531_residues
MNSRRPSIVSGVARTALLCGALFALSPVGSAYAEDLRVGLAADVTSMDPHWNNAGPNNAIALHIFESLVFMDKNARYIPG
LALSWKPVNPTTWEIKLRPNVKWHDGSPFTSADVKASLERPEKLTNSPGSFTSYTKPIARIDTPDALTVRLTMNVANYAN
LANDLNSVPIMPKKVAATLTQADFDSGKAMIGTGPFKFVRFARGQEIVMARNPDYWGAKPEWDRAIFRIITDNGARTSAL
LAGDVDVIESVPAADVAKLKQNPKFRIEQTVSWRTIFWQMDQSRDNPPYVTDKAGKPLGKNPFKDARVRAAISKALNRDA
IVSRIMEGLAVPASSLVSPQIFGHPGTKPDAYDPEGAKKLLAAAGYPNGFGLTLHATNNRYLNDAAVAQATASMLTRVGI
QTKVETLPVAAYFTRARQGDFSFEMLGWGSAAADVALRSIAGTPDPKTGYGTWNWGKYSNPQLDKLIEKSLTTVSSDKAR
EDNAKAAAKFALDDHAVIPSHSQLAMWAMKTGLKYEARTDEWSLAQFFHKQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=537, Percent_Identity=27.56052141527, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1789966, Length=526, Percent_Identity=26.8060836501901, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787762, Length=509, Percent_Identity=27.7013752455796, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1787495, Length=538, Percent_Identity=24.907063197026, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87081878, Length=533, Percent_Identity=24.765478424015, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789887, Length=438, Percent_Identity=23.7442922374429, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789397, Length=527, Percent_Identity=23.9089184060721, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787551, Length=497, Percent_Identity=23.5412474849095, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57926; Mature: 57926

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRRPSIVSGVARTALLCGALFALSPVGSAYAEDLRVGLAADVTSMDPHWNNAGPNNAI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCEE
ALHIFESLVFMDKNARYIPGLALSWKPVNPTTWEIKLRPNVKWHDGSPFTSADVKASLER
EHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHC
PEKLTNSPGSFTSYTKPIARIDTPDALTVRLTMNVANYANLANDLNSVPIMPKKVAATLT
CHHHCCCCCCCCHHCCCHHHCCCCCEEEEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHH
QADFDSGKAMIGTGPFKFVRFARGQEIVMARNPDYWGAKPEWDRAIFRIITDNGARTSAL
HCCCCCCCEEEECCHHHHHHHCCCCEEEEECCCCCCCCCCCHHCEEEEEEECCCCCEEEE
LAGDVDVIESVPAADVAKLKQNPKFRIEQTVSWRTIFWQMDQSRDNPPYVTDKAGKPLGK
EECCHHHHHCCCCHHHHHHHCCCCEEEEECCEEEEEEEEECCCCCCCCEEECCCCCCCCC
NPFKDARVRAAISKALNRDAIVSRIMEGLAVPASSLVSPQIFGHPGTKPDAYDPEGAKKL
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCEECCCCCCCCCCCCHHHHHH
LAAAGYPNGFGLTLHATNNRYLNDAAVAQATASMLTRVGIQTKVETLPVAAYFTRARQGD
HHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
FSFEMLGWGSAAADVALRSIAGTPDPKTGYGTWNWGKYSNPQLDKLIEKSLTTVSSDKAR
CEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
EDNAKAAAKFALDDHAVIPSHSQLAMWAMKTGLKYEARTDEWSLAQFFHKQ
CCCHHHHHEEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MNSRRPSIVSGVARTALLCGALFALSPVGSAYAEDLRVGLAADVTSMDPHWNNAGPNNAI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCEE
ALHIFESLVFMDKNARYIPGLALSWKPVNPTTWEIKLRPNVKWHDGSPFTSADVKASLER
EHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHC
PEKLTNSPGSFTSYTKPIARIDTPDALTVRLTMNVANYANLANDLNSVPIMPKKVAATLT
CHHHCCCCCCCCHHCCCHHHCCCCCEEEEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHH
QADFDSGKAMIGTGPFKFVRFARGQEIVMARNPDYWGAKPEWDRAIFRIITDNGARTSAL
HCCCCCCCEEEECCHHHHHHHCCCCEEEEECCCCCCCCCCCHHCEEEEEEECCCCCEEEE
LAGDVDVIESVPAADVAKLKQNPKFRIEQTVSWRTIFWQMDQSRDNPPYVTDKAGKPLGK
EECCHHHHHCCCCHHHHHHHCCCCEEEEECCEEEEEEEEECCCCCCCCEEECCCCCCCCC
NPFKDARVRAAISKALNRDAIVSRIMEGLAVPASSLVSPQIFGHPGTKPDAYDPEGAKKL
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCEECCCCCCCCCCCCHHHHHH
LAAAGYPNGFGLTLHATNNRYLNDAAVAQATASMLTRVGIQTKVETLPVAAYFTRARQGD
HHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
FSFEMLGWGSAAADVALRSIAGTPDPKTGYGTWNWGKYSNPQLDKLIEKSLTTVSSDKAR
CEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
EDNAKAAAKFALDDHAVIPSHSQLAMWAMKTGLKYEARTDEWSLAQFFHKQ
CCCHHHHHEEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA