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The map label for this gene is gyaR [H]

Identifier: 73541968

GI number: 73541968

Start: 2514465

End: 2515460

Strand: Reverse

Name: gyaR [H]

Synonym: Reut_A2281

Alternate gene names: 73541968

Gene position: 2515460-2514465 (Counterclockwise)

Preceding gene: 73541969

Following gene: 73541967

Centisome position: 66.08

GC content: 67.17

Gene sequence:

>996_bases
ATGAAGCCGTCCATTCTTGTCACCCGTGCCATTTTTCCCGATGTCATCGACCATCTTGCGCAGTACTTCGAGGTCGACGA
CAACCAGCAGGATGTCGTACTCGACGCTGCGGCGCTCAAGGCGCGTCTGGCCGGCAAGGCGGGCGTGCTCACCAACGCTG
GCGACCGCATCGATGGCGGCGTGGTCGCCGCACTGCCCGACCTGCGTGCCGTCTGCAATATGGCGGTCGGCTACAACAAC
CTCGACATCCCCGCGCTGACGGCGGCGGGCATCGTCGCCACCAATACGCCCGATGTGCTGACGGAGACCACGGCCGACTT
CGGCTGGGCGCTGCTGATGGCCACCGCGCGCCGCGTGACCGAGTCCGAGCACTACCTGCGCGATGGCAGGTGGCAGCGGT
GGAGCTACGACATGCTGGTCGGCATGGACGTGTACGGCAGCACGCTGGGTATCCTCGGCATGGGGCGGATCGGCCAGGCG
CTCGCACGCCGCGCGGCCGGGTTTGGCATGACCGTGCTCTATCACAACCGCAGCCAGCTGCCCGAGGCGACCGAGCGCGC
GCTCAACGCGCGCTATGTGAGCAAGGCCGAGTTGCTGCAGCAGTCCGATCACCTGGTGCTGGTGCTGCCATACGGGCCGG
AAAGCCACCATGCCATCGGCGCCACGGAGCTGACCCAGATGAAGCCGACGGCCACGCTGGTCAACCTTGCGCGCGGCGGT
ATCGTCGATGACGAGGCGCTGGCCCATGCGCTCAAGACTCGCCGCATCTTTGCCGCAGGCCTCGACGTGTTCGAAGGCGA
GCCCGATGTCCATCCGGACCTGCTGACCGTGTCGAACGTGGTGCTGACGCCGCACATCGCCAGCGCATCCGAGAAGACGC
GCCGCGCCATGGCGATGCTAGCCGCGGACAACCTGATCGCCGCGCTCGACGCCGGTCCCAATGCCGGCCATCCGCCTTCG
GTGATCGACCCTGCCGTGATGGGGCATCGCCGCTGA

Upstream 100 bases:

>100_bases
CTGCGCATGCCCAGTTTCTTCGGCTACATGCTATGGTCTGGCTCCATCCTGTTGCCGCTGTTCCTGGTGATGACGTTCCT
CTTTTTCCGTGTGTGATGCC

Downstream 100 bases:

>100_bases
AGTTTTACTCATGACCTGGATTCCCTTGCTGACGCTGGGCGTAGCGCTGCTGGCGCTCGCCCTGCTTGTCGTTCTGCTGC
TGCGCCAGCAGGCGACACCT

Product: 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKPSILVTRAIFPDVIDHLAQYFEVDDNQQDVVLDAAALKARLAGKAGVLTNAGDRIDGGVVAALPDLRAVCNMAVGYNN
LDIPALTAAGIVATNTPDVLTETTADFGWALLMATARRVTESEHYLRDGRWQRWSYDMLVGMDVYGSTLGILGMGRIGQA
LARRAAGFGMTVLYHNRSQLPEATERALNARYVSKAELLQQSDHLVLVLPYGPESHHAIGATELTQMKPTATLVNLARGG
IVDDEALAHALKTRRIFAAGLDVFEGEPDVHPDLLTVSNVVLTPHIASASEKTRRAMAMLAADNLIAALDAGPNAGHPPS
VIDPAVMGHRR

Sequences:

>Translated_331_residues
MKPSILVTRAIFPDVIDHLAQYFEVDDNQQDVVLDAAALKARLAGKAGVLTNAGDRIDGGVVAALPDLRAVCNMAVGYNN
LDIPALTAAGIVATNTPDVLTETTADFGWALLMATARRVTESEHYLRDGRWQRWSYDMLVGMDVYGSTLGILGMGRIGQA
LARRAAGFGMTVLYHNRSQLPEATERALNARYVSKAELLQQSDHLVLVLPYGPESHHAIGATELTQMKPTATLVNLARGG
IVDDEALAHALKTRRIFAAGLDVFEGEPDVHPDLLTVSNVVLTPHIASASEKTRRAMAMLAADNLIAALDAGPNAGHPPS
VIDPAVMGHRR
>Mature_331_residues
MKPSILVTRAIFPDVIDHLAQYFEVDDNQQDVVLDAAALKARLAGKAGVLTNAGDRIDGGVVAALPDLRAVCNMAVGYNN
LDIPALTAAGIVATNTPDVLTETTADFGWALLMATARRVTESEHYLRDGRWQRWSYDMLVGMDVYGSTLGILGMGRIGQA
LARRAAGFGMTVLYHNRSQLPEATERALNARYVSKAELLQQSDHLVLVLPYGPESHHAIGATELTQMKPTATLVNLARGG
IVDDEALAHALKTRRIFAAGLDVFEGEPDVHPDLLTVSNVVLTPHIASASEKTRRAMAMLAADNLIAALDAGPNAGHPPS
VIDPAVMGHRR

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=294, Percent_Identity=38.7755102040816, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI4557497, Length=249, Percent_Identity=38.1526104417671, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI61743967, Length=249, Percent_Identity=38.1526104417671, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI23308577, Length=289, Percent_Identity=28.3737024221453, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI145580578, Length=249, Percent_Identity=37.7510040160643, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI4557499, Length=249, Percent_Identity=37.7510040160643, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI145580575, Length=249, Percent_Identity=37.7510040160643, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI87082289, Length=314, Percent_Identity=43.9490445859873, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1789279, Length=258, Percent_Identity=31.0077519379845, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787645, Length=245, Percent_Identity=29.7959183673469, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17532191, Length=259, Percent_Identity=29.7297297297297, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=262, Percent_Identity=28.6259541984733, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=259, Percent_Identity=35.9073359073359, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6320925, Length=276, Percent_Identity=29.3478260869565, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=265, Percent_Identity=29.0566037735849, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321253, Length=164, Percent_Identity=32.9268292682927, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325144, Length=144, Percent_Identity=30.5555555555556, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324964, Length=226, Percent_Identity=25.6637168141593, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI45551003, Length=314, Percent_Identity=36.9426751592357, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI28574284, Length=314, Percent_Identity=36.9426751592357, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45552429, Length=314, Percent_Identity=36.9426751592357, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24585514, Length=314, Percent_Identity=36.9426751592357, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28574282, Length=314, Percent_Identity=36.9426751592357, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28571528, Length=270, Percent_Identity=42.2222222222222, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28574286, Length=304, Percent_Identity=36.8421052631579, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24585516, Length=260, Percent_Identity=32.3076923076923, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24646446, Length=268, Percent_Identity=34.3283582089552, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646448, Length=268, Percent_Identity=34.3283582089552, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646452, Length=268, Percent_Identity=34.3283582089552, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646450, Length=268, Percent_Identity=34.3283582089552, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI62472511, Length=249, Percent_Identity=34.9397590361446, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI19921140, Length=266, Percent_Identity=29.3233082706767, Blast_Score=127, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 35465; Mature: 35465

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSILVTRAIFPDVIDHLAQYFEVDDNQQDVVLDAAALKARLAGKAGVLTNAGDRIDGG
CCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCC
VVAALPDLRAVCNMAVGYNNLDIPALTAAGIVATNTPDVLTETTADFGWALLMATARRVT
EEEECHHHHHHHHHHCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
ESEHYLRDGRWQRWSYDMLVGMDVYGSTLGILGMGRIGQALARRAAGFGMTVLYHNRSQL
HHHHHHHCCCCCEEEEEEEEECHHHCCHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCC
PEATERALNARYVSKAELLQQSDHLVLVLPYGPESHHAIGATELTQMKPTATLVNLARGG
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHCCC
IVDDEALAHALKTRRIFAAGLDVFEGEPDVHPDLLTVSNVVLTPHIASASEKTRRAMAML
CCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHH
AADNLIAALDAGPNAGHPPSVIDPAVMGHRR
HHCCEEEEEECCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MKPSILVTRAIFPDVIDHLAQYFEVDDNQQDVVLDAAALKARLAGKAGVLTNAGDRIDGG
CCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCC
VVAALPDLRAVCNMAVGYNNLDIPALTAAGIVATNTPDVLTETTADFGWALLMATARRVT
EEEECHHHHHHHHHHCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
ESEHYLRDGRWQRWSYDMLVGMDVYGSTLGILGMGRIGQALARRAAGFGMTVLYHNRSQL
HHHHHHHCCCCCEEEEEEEEECHHHCCHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCC
PEATERALNARYVSKAELLQQSDHLVLVLPYGPESHHAIGATELTQMKPTATLVNLARGG
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHCCC
IVDDEALAHALKTRRIFAAGLDVFEGEPDVHPDLLTVSNVVLTPHIASASEKTRRAMAML
CCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHH
AADNLIAALDAGPNAGHPPSVIDPAVMGHRR
HHCCEEEEEECCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA