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The map label for this gene is rpoE [H]

Identifier: 73541946

GI number: 73541946

Start: 2492460

End: 2493059

Strand: Reverse

Name: rpoE [H]

Synonym: Reut_A2259

Alternate gene names: 73541946

Gene position: 2493059-2492460 (Counterclockwise)

Preceding gene: 73541947

Following gene: 73541945

Centisome position: 65.49

GC content: 59.5

Gene sequence:

>600_bases
GTGAGCGAACGCGAAGCCGATCAGCTCCTAGTTGAACGCGTCCAGCAGGGCGACAAGCGGGCCTTTGAGCTGCTGGTGGC
AAAGTACCACCGGAAGATCATCCGCCTGATTTCGCGCCTGGTGAGAGATCCCGCCGAAGTCGAGGATGTGGCGCAGGATG
CCTTTATCAAGGCATATCGTGCCTTGCCCCAATTCCGCGGGGAATCCGCCTTCTACACATGGCTGTACCGGATCGCCGTG
AACACGGCCAAGAACTATCTGGCTACGCAGGGACGACGACCCGAGGCGTCCACCGACATCGATACGGAAGAGGCTGAAAC
TTTTGCGGACGGTGAGCAACTAAGGGATATCAATACGCCGGAGTCGATGCTTCACACGCGCCAAGTCGCCGAAACGGTGA
ACCGAGCGATGGAAGCGTTGCCGGAAGAGTTGCGTACCGCCATCACCCTGCGCGAGATCGAAGGCCTCAGCTATGAGGAA
ATCGCGGAGGCGATGGGTTGCCCGATCGGAACGGTGCGTTCACGCATTTTCCGTGCGCGTGAGGCGATTGCTGAAAAATT
GCGCCCGCTGCTGGGAACGGCAGAGGGCAAGCGATGGTAG

Upstream 100 bases:

>100_bases
CAGCATGCACTTTCAAGCACTGGGCGATCGCAGTTCGTAGGTAAAATACAACGTTCGTCGTGACGACCAAACCAAGAGAT
ACACTGCAGGTGAACCATAC

Downstream 100 bases:

>100_bases
TGAGAAGAATGTCGTGCGGCCAGCGTTGCAGCCTATTCCTGCATCGATAAGCGACAGCAAGTGACGGGATTATCGGTGTT
CAATGGAATTGGAATGTCTT

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSEREADQLLVERVQQGDKRAFELLVAKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRPEASTDIDTEEAETFADGEQLRDINTPESMLHTRQVAETVNRAMEALPEELRTAITLREIEGLSYEE
IAEAMGCPIGTVRSRIFRAREAIAEKLRPLLGTAEGKRW

Sequences:

>Translated_199_residues
MSEREADQLLVERVQQGDKRAFELLVAKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRPEASTDIDTEEAETFADGEQLRDINTPESMLHTRQVAETVNRAMEALPEELRTAITLREIEGLSYEE
IAEAMGCPIGTVRSRIFRAREAIAEKLRPLLGTAEGKRW
>Mature_198_residues
SEREADQLLVERVQQGDKRAFELLVAKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAVN
TAKNYLATQGRRPEASTDIDTEEAETFADGEQLRDINTPESMLHTRQVAETVNRAMEALPEELRTAITLREIEGLSYEEI
AEAMGCPIGTVRSRIFRAREAIAEKLRPLLGTAEGKRW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=61.7801047120419, Blast_Score=242, Evalue=1e-65,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22783; Mature: 22652

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEREADQLLVERVQQGDKRAFELLVAKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRPEASTDIDTEEAETFADGEQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
ESMLHTRQVAETVNRAMEALPEELRTAITLREIEGLSYEEIAEAMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAEKLRPLLGTAEGKRW
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEREADQLLVERVQQGDKRAFELLVAKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRPEASTDIDTEEAETFADGEQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
ESMLHTRQVAETVNRAMEALPEELRTAITLREIEGLSYEEIAEAMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAEKLRPLLGTAEGKRW
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]