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The map label for this gene is phaA [H]

Identifier: 73541826

GI number: 73541826

Start: 2357468

End: 2359207

Strand: Reverse

Name: phaA [H]

Synonym: Reut_A2138

Alternate gene names: 73541826

Gene position: 2359207-2357468 (Counterclockwise)

Preceding gene: 73541827

Following gene: 73541825

Centisome position: 61.98

GC content: 65.34

Gene sequence:

>1740_bases
ATGACAACAGCCGTGGGTACGAGAACGCTGGCGAAGACGACAAAGAAGGCGAAGAAGCCGGCAGCCAGTGCTGTCGCTCC
GGAGGCAATTGTTGACAGCGCGCCGGCCATGACGGGACCGAACCCGTTCGTGGGGCTGCGCCCCGAAGATATGGTCAGCA
CCGCGCAGCAGATCGGCGCGCAGTGCGTGCAGGAACCGACCTTGCTGCTGCAGCAGCAGACCAAGCTCGCGCAGGACCTG
TTCGCGGTCGTGGACGGCTCGGCGCCGCCGCCGTGCACGCGTGACCGCCGCTTCGGCGATCCGGCATGGCAGGATAACCC
GGTCTATCGCATGGCCCTGCAGGGATATCAGGCCTGGCGCGATGCGCTGACCGGCTTCGTCACCGGCTCGGTCATGGACG
GTCGCAGCAAGGAACGCGCGCAGTACTTCACCGACCTGCTGATCGACGCCGTGGCGCCGACCAATACGCTACTGGGGAAT
CCGGCTGCATTGCGGAAGACGTTCGAGTCGGGTGGTCTGAACCTGGTCAGCGGCCTGGTCAACCTGTTGACCGATACGCT
GACGAACCAGGGCATGCCGTCCCAGGTGGACAAGAGCGCGTTCCGTATCGGCAAGGATCTGGCCGCCACGCCAGGCGCGG
TGGTTTTCCGCAACGATGTCCTGGAGCTGATCCAGTACACGCCGACCACGGCAAACGTGCATGCCCGTCCGCAGCTCATC
GTGCCGCCGCAGGTCAACAAGTTCTATGTGTTTGACCTGTCGCAGGGCAAGAGCATGGTCGAGTACCTGGTGCGCAATGA
GATGCAGGTGTTCATGGTGAGCTGGCGCAATCCCACCACGTTGCACCGCGACTGGAACCTCGACACCTATGTCGGTGCGC
TGATCGAAGCGATCCACGCGATCCGCGACATCACTGGCAGCCCCGACGTGAACCTGCATGGCGCGTGCTCGGGCGCGATG
ACGATGTCCGCGCTCATGGGGTACTGCGCAGCGCGCGGCGAAAAGCTCGTGAATGCCGCCACGCTGATGGTGTCCGTGTT
CGGCCTCGACTCGCAGTCCCAGCTCGGACTGTTTGCCACGCCGGAAGCCATCGATGCCGCGCGGCAGGGCAGCCAGGCGC
GCGGTGTGCTGGATGGCGGGGAACTCGGGCGCGTGTTCGCCTGGATGCGGCCCAATGACCTGGTGTGGAACTACTGGGTC
AACAACTACCTGCTGGGCAAGGCGCCGCCGGCGTTCGACATCCTCTACTGGAACAACGACACCACGCGCCTGCCCGCCGG
CCTGCACGGCCAGTTGCTGGACATGCTGACGCTGAACCAGCTGTCGACACCGGGCGCGCTGCGCGTGCTTGGCCAGCCGA
TCGACCTTTCGGCTGTTGATTGCGACAAGTATGTGCTTGCCGGCATGACCGACCACATCACGCCGTGGAAGGGCGTGTAC
CGCTCGGTGCGCGCGTTCGGCGGCAAGACCGAGTTCGTGCTCAGCTCCAGCGGTCATGTGCAGAGCCTCATCAACCCGCC
GGGAAATGCCAAGGCGAAGTTCTTCCTGAACGACGCGCAGATGGCGGACCCGGAAGCGTGGCTCGAGGGTGCCACGGTCA
CCCCGTCGTCCTGGTGGGAGCACTGGAGCGAGTGGCTGAAAACGCGTTCTGGCCCCCTGAACGCCGCGCCGTCCCGCCTG
GGCAGCGACCGCCATGCGCCATGCGCCGACGCGCCGGGACGCTATGTGACCGAGGCGTAA

Upstream 100 bases:

>100_bases
ATCGAAGGCGAGGGCAAGCCCGCGCTGGTGGCTGAAGCGCTTGCGCTGCTGGTCGCCTGAGGATCGAACAAGGTACGAAC
AAGAAGACGAGGGTAGTAGC

Downstream 100 bases:

>100_bases
GCCCGGCCACCGATTTCTGTAACGCCAACTGTAATCTGTAAGGAGAGTAAAAAATGGAAGCCACCAATGCCGCAGCCGCT
GCGTCCCGCAAGCTGTTCGA

Product: Alpha/beta hydrolase fold:Poly-beta-hydroxybutyrate polymerase, N-terminal

Products: NA

Alternate protein names: PHA polymerase 1; PHA synthase 1; Polyhydroxyalkanoic acid synthase 1 [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MTTAVGTRTLAKTTKKAKKPAASAVAPEAIVDSAPAMTGPNPFVGLRPEDMVSTAQQIGAQCVQEPTLLLQQQTKLAQDL
FAVVDGSAPPPCTRDRRFGDPAWQDNPVYRMALQGYQAWRDALTGFVTGSVMDGRSKERAQYFTDLLIDAVAPTNTLLGN
PAALRKTFESGGLNLVSGLVNLLTDTLTNQGMPSQVDKSAFRIGKDLAATPGAVVFRNDVLELIQYTPTTANVHARPQLI
VPPQVNKFYVFDLSQGKSMVEYLVRNEMQVFMVSWRNPTTLHRDWNLDTYVGALIEAIHAIRDITGSPDVNLHGACSGAM
TMSALMGYCAARGEKLVNAATLMVSVFGLDSQSQLGLFATPEAIDAARQGSQARGVLDGGELGRVFAWMRPNDLVWNYWV
NNYLLGKAPPAFDILYWNNDTTRLPAGLHGQLLDMLTLNQLSTPGALRVLGQPIDLSAVDCDKYVLAGMTDHITPWKGVY
RSVRAFGGKTEFVLSSSGHVQSLINPPGNAKAKFFLNDAQMADPEAWLEGATVTPSSWWEHWSEWLKTRSGPLNAAPSRL
GSDRHAPCADAPGRYVTEA

Sequences:

>Translated_579_residues
MTTAVGTRTLAKTTKKAKKPAASAVAPEAIVDSAPAMTGPNPFVGLRPEDMVSTAQQIGAQCVQEPTLLLQQQTKLAQDL
FAVVDGSAPPPCTRDRRFGDPAWQDNPVYRMALQGYQAWRDALTGFVTGSVMDGRSKERAQYFTDLLIDAVAPTNTLLGN
PAALRKTFESGGLNLVSGLVNLLTDTLTNQGMPSQVDKSAFRIGKDLAATPGAVVFRNDVLELIQYTPTTANVHARPQLI
VPPQVNKFYVFDLSQGKSMVEYLVRNEMQVFMVSWRNPTTLHRDWNLDTYVGALIEAIHAIRDITGSPDVNLHGACSGAM
TMSALMGYCAARGEKLVNAATLMVSVFGLDSQSQLGLFATPEAIDAARQGSQARGVLDGGELGRVFAWMRPNDLVWNYWV
NNYLLGKAPPAFDILYWNNDTTRLPAGLHGQLLDMLTLNQLSTPGALRVLGQPIDLSAVDCDKYVLAGMTDHITPWKGVY
RSVRAFGGKTEFVLSSSGHVQSLINPPGNAKAKFFLNDAQMADPEAWLEGATVTPSSWWEHWSEWLKTRSGPLNAAPSRL
GSDRHAPCADAPGRYVTEA
>Mature_578_residues
TTAVGTRTLAKTTKKAKKPAASAVAPEAIVDSAPAMTGPNPFVGLRPEDMVSTAQQIGAQCVQEPTLLLQQQTKLAQDLF
AVVDGSAPPPCTRDRRFGDPAWQDNPVYRMALQGYQAWRDALTGFVTGSVMDGRSKERAQYFTDLLIDAVAPTNTLLGNP
AALRKTFESGGLNLVSGLVNLLTDTLTNQGMPSQVDKSAFRIGKDLAATPGAVVFRNDVLELIQYTPTTANVHARPQLIV
PPQVNKFYVFDLSQGKSMVEYLVRNEMQVFMVSWRNPTTLHRDWNLDTYVGALIEAIHAIRDITGSPDVNLHGACSGAMT
MSALMGYCAARGEKLVNAATLMVSVFGLDSQSQLGLFATPEAIDAARQGSQARGVLDGGELGRVFAWMRPNDLVWNYWVN
NYLLGKAPPAFDILYWNNDTTRLPAGLHGQLLDMLTLNQLSTPGALRVLGQPIDLSAVDCDKYVLAGMTDHITPWKGVYR
SVRAFGGKTEFVLSSSGHVQSLINPPGNAKAKFFLNDAQMADPEAWLEGATVTPSSWWEHWSEWLKTRSGPLNAAPSRLG
SDRHAPCADAPGRYVTEA

Specific function: P.oleovorans accumulates poly(3-hydroxyalkanoates) after growth on medium chain length hydrocarbons. Large amounts of this polyester are synthesized when cells are grown under nitrogen- limiting conditions. When nitrogen is resupplied in the medium, the a

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011287
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 62849; Mature: 62718

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTAVGTRTLAKTTKKAKKPAASAVAPEAIVDSAPAMTGPNPFVGLRPEDMVSTAQQIGA
CCCCCHHHHHHHHHHHHCCHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QCVQEPTLLLQQQTKLAQDLFAVVDGSAPPPCTRDRRFGDPAWQDNPVYRMALQGYQAWR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
DALTGFVTGSVMDGRSKERAQYFTDLLIDAVAPTNTLLGNPAALRKTFESGGLNLVSGLV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCHHHHHHHH
NLLTDTLTNQGMPSQVDKSAFRIGKDLAATPGAVVFRNDVLELIQYTPTTANVHARPQLI
HHHHHHHHCCCCCHHHHHHHHHHCCHHCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEE
VPPQVNKFYVFDLSQGKSMVEYLVRNEMQVFMVSWRNPTTLHRDWNLDTYVGALIEAIHA
ECCCCCEEEEEECCCCHHHHHHHHHCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHH
IRDITGSPDVNLHGACSGAMTMSALMGYCAARGEKLVNAATLMVSVFGLDSQSQLGLFAT
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEC
PEAIDAARQGSQARGVLDGGELGRVFAWMRPNDLVWNYWVNNYLLGKAPPAFDILYWNND
CHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCEEECCCCCCEEEEEECCC
TTRLPAGLHGQLLDMLTLNQLSTPGALRVLGQPIDLSAVDCDKYVLAGMTDHITPWKGVY
CCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCEECCCCHHHHHCCCCCCCCHHHHH
RSVRAFGGKTEFVLSSSGHVQSLINPPGNAKAKFFLNDAQMADPEAWLEGATVTPSSWWE
HHHHHCCCCEEEEEECCCCHHHHCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCHHHHH
HWSEWLKTRSGPLNAAPSRLGSDRHAPCADAPGRYVTEA
HHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]