Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 73541777

GI number: 73541777

Start: 2296980

End: 2298134

Strand: Reverse

Name: rlmN [H]

Synonym: Reut_A2089

Alternate gene names: 73541777

Gene position: 2298134-2296980 (Counterclockwise)

Preceding gene: 73541778

Following gene: 73541776

Centisome position: 60.37

GC content: 63.72

Gene sequence:

>1155_bases
ATGAACGATCTCGTCAACCTGCTCGACCTGGATGCGGACGCGCTCACTGCCTATTGCGGCGAGCTCGGCGAGAAGCCGTT
TCGTGCGCGGCAGCTGCAACGCTGGATCCACCAGTTCGGTGCCAGCCGTTTCGACGCCATGTCGGACCTCGCCAAGTCGC
TGCGCGAAAAGCTCGCGACGCGCGCCGAGATCCGTTCGCCGGCCGCCATCACCGACAACCTGTCGGCTGACGGCACGCGC
AAGTGGCTGCTCGATGTCGGTAACGGCAACGCGGTGGAGACGGTGTACATCCCCGAGGAAACGCGCGGCACGCTGTGCGT
CTCCTCGCAGGCGGGGTGCGCTGTCAACTGCCGGTTCTGCTCGACCGGCAAGCAGGGTTTCTCGCGCAATCTGACTACGG
GCGAAATCATTGGCCAGTTGTGGATGGCGGAATTCGCCATGCGCGAACAGCTGGGCCGTGGGCCCAAGGACGACCGCGTC
ATCTCCAACGTGGTGATGATGGGTATGGGCGAGCCGCTGCTGAACTACGATGCCGTCGTGCCGGCCATGCGGCTGATGCT
CGACGATAACGCGTATGGCCTGTCGCGCCGCCGCGTGACGCTGTCCACTTCCGGCGTGGTGCCGATGATGGACCGTCTGT
CGAAAGACCTGCCGGTAGCGCTGGCCGTGTCTCTGCACGCGTCGAACGACGCCCTGCGCGACGTGCTCGTGCCGCTGAAC
AAGAAGTACCCGCTGGCCGAACTGATGGCGGCATGCCGCCGTTATCTGGAATTCGCCCCGCGCGATTTCATTACTTTCGA
ATACTGCATGCTCGATGGCGTCAATGACGGCGTCGAACATGCGCGGGAACTGTTGAAGCTTGTGGCCGACGTGCCGTGCA
AGTTCAACCTGATCCCGTTCAATCCGTTCCCTGAATCGGGTTTGAAGCGTTCCAACAACGATCAGATCCGGCGCTTTGCG
CAGGTACTGATGGATGCGGGCATCGTGACCACGATCCGCAAGACGCGCGGCGACGATATCGACGCGGCATGCGGCCAGCT
TGCCGGGGAGGTCAAGGATCGCACCCGGCTTGCCGAGCGGGGCAAGTTCGGCAAGATCACGCCTTTGGTGCCGGTCGCCG
CGACTGGCCAAGCCGGGGAGGCTCGCCCTGCATGA

Upstream 100 bases:

>100_bases
TGTGGAAATTGCCTTCTTCTTCCCGGGCATGAACGTTTATACCCGTTGATTGTCTGGTCAATTCCAAGACTAGACAGACA
CGGAAGCTGAGTTTGCCGTC

Downstream 100 bases:

>100_bases
TCCGTCTGATCGCTGCAGCCCTGTTGGGGCTGCTGATGTTGTCCGCCTGCCAGTTGCCGCCCGCACCGACGCAGGACCTC
CAGACTACTTCCGACCAGAC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNDLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASRFDAMSDLAKSLREKLATRAEIRSPAAITDNLSADGTR
KWLLDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLTTGEIIGQLWMAEFAMREQLGRGPKDDRV
ISNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPFNPFPESGLKRSNNDQIRRFA
QVLMDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAERGKFGKITPLVPVAATGQAGEARPA

Sequences:

>Translated_384_residues
MNDLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASRFDAMSDLAKSLREKLATRAEIRSPAAITDNLSADGTR
KWLLDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLTTGEIIGQLWMAEFAMREQLGRGPKDDRV
ISNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPFNPFPESGLKRSNNDQIRRFA
QVLMDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAERGKFGKITPLVPVAATGQAGEARPA
>Mature_384_residues
MNDLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASRFDAMSDLAKSLREKLATRAEIRSPAAITDNLSADGTR
KWLLDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLTTGEIIGQLWMAEFAMREQLGRGPKDDRV
ISNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPFNPFPESGLKRSNNDQIRRFA
QVLMDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAERGKFGKITPLVPVAATGQAGEARPA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=361, Percent_Identity=55.9556786703601, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42247; Mature: 42247

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASRFDAMSDLAKSLREKLAT
CCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RAEIRSPAAITDNLSADGTRKWLLDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCRFC
HHHHCCCCHHCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEE
STGKQGFSRNLTTGEIIGQLWMAEFAMREQLGRGPKDDRVISNVVMMGMGEPLLNYDAVV
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
PAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
HHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHCCC
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPF
CCCCHHHHHHHHHHHHHHCCCHHHEEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
NPFPESGLKRSNNDQIRRFAQVLMDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAER
CCCCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GKFGKITPLVPVAATGQAGEARPA
CCCCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MNDLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASRFDAMSDLAKSLREKLAT
CCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RAEIRSPAAITDNLSADGTRKWLLDVGNGNAVETVYIPEETRGTLCVSSQAGCAVNCRFC
HHHHCCCCHHCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEE
STGKQGFSRNLTTGEIIGQLWMAEFAMREQLGRGPKDDRVISNVVMMGMGEPLLNYDAVV
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
PAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLN
HHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHCCC
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPF
CCCCHHHHHHHHHHHHHHCCCHHHEEHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
NPFPESGLKRSNNDQIRRFAQVLMDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAER
CCCCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GKFGKITPLVPVAATGQAGEARPA
CCCCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA