Click here to switch to the map view.

The map label for this gene is purA [H]

Identifier: 73541764

GI number: 73541764

Start: 2281767

End: 2283107

Strand: Reverse

Name: purA [H]

Synonym: Reut_A2076

Alternate gene names: 73541764

Gene position: 2283107-2281767 (Counterclockwise)

Preceding gene: 73541765

Following gene: 73541763

Centisome position: 59.98

GC content: 64.58

Gene sequence:

>1341_bases
ATGTCCGCATCCGCAGTAGGCCAGGGACGCAACGTCGTCGTGATCGGCACCCAGTGGGGTGACGAAGGCAAGGGGAAAAT
CGTCGATTGGCTTACCGATCATGCAAAAGGCGTGGTGCGGTTCCAGGGCGGCCACAACGCTGGCCACACCCTGATCATCG
GCGGCAAGAAGACCATCCTCCGACTGATCCCGTCGGGAATCATGCGTGAAGGCACCGTCTGCTACATCGGCAACGGTGTG
GTGCTGTCGCCGGAAGCCCTGTTCCGCGAAATCGAAGAACTCGAGACGGCTGGCCTGGAAGTGCAGAAGCGTCTGCGCAT
TTCGGAAGCCGCAACGCTGATCCTGCCGTACCACGTGGCCATCGACAAGGCACGCGAAGCGCGCCGCGGTGCCGCCAAGA
TCGGCACGACCGGCCGTGGCATTGGCCCTGCTTACGAAGACAAGGTGGCACGCCGCGCGCTGCGCGTGCAGGACCTGTTC
GATCCGCAACAGTTCGCCGAACGCCTGCGCGAAAACCTGGATTTCCACAACTTCATGCTGACGCAGTACCTCGGCGCCGA
AGCCGTGGACTACCAGCAGACGCTCGATGACGCCCTGGCCTTTGCGCCGCGCCTGGCACCGATGGTCGCTGACGTGTCCG
CAGAGCTTTATGCCGTCAATGCTGCCGGTGGCAACCTGATGTTCGAAGGCGCGCAAGGCACGCTGCTCGACGTCGACCAC
GGCACCTATCCGTTCGTGACGTCCAGCAACTGCGTGGCCGGTGCGGCTGCCGCAGGCGCCGGCGTCGGCCCGGGCCGCCT
GAGCTACATCCTGGGCATCACCAAGGCCTACTGCACGCGCGTTGGCGCTGGTCCGTTCCCGAGCGAGCTGTACGACAACG
ACAATCCGGCGCGCCAGGATCAAGTCGGCGTGCGTCTGGCCAACGTCGGCAAGGAATTCGGTTCGGTCACAGGCCGTCCG
CGCCGCACTGGCTGGCTCGATGCCGCCGCGCTCAAGCGCTCGGTGCAGATCAATGGCGTGTCGGGCCTGTGCCTGACCAA
GCTCGACGTGCTTGATGGCCTAGAAAGCATCAAGCTGTGCGTGGGCTACACGCTCGACGGCAAGACCGTGGACATCCTGC
CGCGCGGCTCCGATGCTGTCGCGCGCTGCGAGCCGGTCTACGAGGAATTCCCGGGCTGGAACGAGTCGACCTTTGGCGTG
AAGGCGTGGGATGCGCTGCCGGAAGCGGCGCGTGTCTACCTGAAGCGTGTGGAAGAAGTGGTTGGTATCCCGATCGACAT
GATCTCGACCGGTCCGGACCGCGACGAGACCATCCTGCTGCGTCACCCGTACCTGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCCTCGCTAGGACTTGCCGCCGGGTGGTTGCCCCAAAAAGCGGGAATCGACCGGTAACAAGAGTAGAATACGTTTTT
AACCTATTGACCAAAGCAAT

Downstream 100 bases:

>100_bases
TCCGGCGTACAACGTTGCAACGGCCGCGCGCCACAAGCGCTGCGGCTGGCAAGAACAAATCGGCCCGCGCATGCGGGCCG
AACTATATCGGTCAACCGAC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMREGTVCYIGNGV
VLSPEALFREIEELETAGLEVQKRLRISEAATLILPYHVAIDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLF
DPQQFAERLRENLDFHNFMLTQYLGAEAVDYQQTLDDALAFAPRLAPMVADVSAELYAVNAAGGNLMFEGAQGTLLDVDH
GTYPFVTSSNCVAGAAAAGAGVGPGRLSYILGITKAYCTRVGAGPFPSELYDNDNPARQDQVGVRLANVGKEFGSVTGRP
RRTGWLDAAALKRSVQINGVSGLCLTKLDVLDGLESIKLCVGYTLDGKTVDILPRGSDAVARCEPVYEEFPGWNESTFGV
KAWDALPEAARVYLKRVEEVVGIPIDMISTGPDRDETILLRHPYLA

Sequences:

>Translated_446_residues
MSASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMREGTVCYIGNGV
VLSPEALFREIEELETAGLEVQKRLRISEAATLILPYHVAIDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLF
DPQQFAERLRENLDFHNFMLTQYLGAEAVDYQQTLDDALAFAPRLAPMVADVSAELYAVNAAGGNLMFEGAQGTLLDVDH
GTYPFVTSSNCVAGAAAAGAGVGPGRLSYILGITKAYCTRVGAGPFPSELYDNDNPARQDQVGVRLANVGKEFGSVTGRP
RRTGWLDAAALKRSVQINGVSGLCLTKLDVLDGLESIKLCVGYTLDGKTVDILPRGSDAVARCEPVYEEFPGWNESTFGV
KAWDALPEAARVYLKRVEEVVGIPIDMISTGPDRDETILLRHPYLA
>Mature_445_residues
SASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMREGTVCYIGNGVV
LSPEALFREIEELETAGLEVQKRLRISEAATLILPYHVAIDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFD
PQQFAERLRENLDFHNFMLTQYLGAEAVDYQQTLDDALAFAPRLAPMVADVSAELYAVNAAGGNLMFEGAQGTLLDVDHG
TYPFVTSSNCVAGAAAAGAGVGPGRLSYILGITKAYCTRVGAGPFPSELYDNDNPARQDQVGVRLANVGKEFGSVTGRPR
RTGWLDAAALKRSVQINGVSGLCLTKLDVLDGLESIKLCVGYTLDGKTVDILPRGSDAVARCEPVYEEFPGWNESTFGVK
AWDALPEAARVYLKRVEEVVGIPIDMISTGPDRDETILLRHPYLA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=444, Percent_Identity=41.2162162162162, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI34577063, Length=442, Percent_Identity=40.0452488687783, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI40316944, Length=405, Percent_Identity=39.7530864197531, Blast_Score=288, Evalue=5e-78,
Organism=Escherichia coli, GI1790620, Length=439, Percent_Identity=62.1867881548975, Blast_Score=552, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI25146553, Length=439, Percent_Identity=42.1412300683371, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=42.2018348623853, Blast_Score=326, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=38.4439359267735, Blast_Score=315, Evalue=7e-87,
Organism=Drosophila melanogaster, GI21356033, Length=429, Percent_Identity=38.6946386946387, Blast_Score=299, Evalue=3e-81,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47981; Mature: 47850

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTIL
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHH
RLIPSGIMREGTVCYIGNGVVLSPEALFREIEELETAGLEVQKRLRISEAATLILPYHVA
HHHHHHHHCCCCEEEECCCEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEHHHH
IDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFDPQQFAERLRENLDFHNFML
HHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHH
TQYLGAEAVDYQQTLDDALAFAPRLAPMVADVSAELYAVNAAGGNLMFEGAQGTLLDVDH
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCEEECCC
GTYPFVTSSNCVAGAAAAGAGVGPGRLSYILGITKAYCTRVGAGPFPSELYDNDNPARQD
CCCCEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHH
QVGVRLANVGKEFGSVTGRPRRTGWLDAAALKRSVQINGVSGLCLTKLDVLDGLESIKLC
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCEEEECCCCCHHHHHHHHHHCHHHEEEH
VGYTLDGKTVDILPRGSDAVARCEPVYEEFPGWNESTFGVKAWDALPEAARVYLKRVEEV
EEEEECCCEEEEEECCCCHHHHCCHHHHHCCCCCCCCCCEEHHHHCHHHHHHHHHHHHHH
VGIPIDMISTGPDRDETILLRHPYLA
HCCCCEEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTIL
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHH
RLIPSGIMREGTVCYIGNGVVLSPEALFREIEELETAGLEVQKRLRISEAATLILPYHVA
HHHHHHHHCCCCEEEECCCEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEHHHH
IDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFDPQQFAERLRENLDFHNFML
HHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHH
TQYLGAEAVDYQQTLDDALAFAPRLAPMVADVSAELYAVNAAGGNLMFEGAQGTLLDVDH
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCEEECCC
GTYPFVTSSNCVAGAAAAGAGVGPGRLSYILGITKAYCTRVGAGPFPSELYDNDNPARQD
CCCCEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHH
QVGVRLANVGKEFGSVTGRPRRTGWLDAAALKRSVQINGVSGLCLTKLDVLDGLESIKLC
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCEEEECCCCCHHHHHHHHHHCHHHEEEH
VGYTLDGKTVDILPRGSDAVARCEPVYEEFPGWNESTFGVKAWDALPEAARVYLKRVEEV
EEEEECCCEEEEEECCCCHHHHCCHHHHHCCCCCCCCCCEEHHHHCHHHHHHHHHHHHHH
VGIPIDMISTGPDRDETILLRHPYLA
HCCCCEEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA