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The map label for this gene is cysT [H]

Identifier: 73541658

GI number: 73541658

Start: 2156668

End: 2157624

Strand: Reverse

Name: cysT [H]

Synonym: Reut_A1970

Alternate gene names: 73541658

Gene position: 2157624-2156668 (Counterclockwise)

Preceding gene: 73541659

Following gene: 73541657

Centisome position: 56.68

GC content: 64.79

Gene sequence:

>957_bases
ATGCCACCGACCATTTCCCTGGCGGACACGCCCCCACCGGCCGCCCCCGATTCGCGTGCCAGCCGCAGCGCGCCGGGCCG
TAACAGCCGCCAGCGCTTTACGGTATTGCCAGGCTTTGGCCTGTCCCTCGGTTTCACGATTTTCTATCTCACGCTGATCG
TGCTGGTCCCGCTGTCGGCCACGTTCCTGAAGACGTTCACGATGACGTGGGAGGCCTTTTGGACGACGGTGACGGCGCCA
CGCGTGGTCGCCTCGTATCAACTGAGCTTTGGTGCTTCGCTGATCGCCGCCATCATCAATACGGTCTTCGGGCTGGTGGT
CGCGTGGGTGCTGGTGCGCTACCGCTTTCCGGGCAAGCGCCTGATCGATGCGCTGGTGGACCTGCCGTTCGCGCTGCCCA
CGGCCGTGGCTGGTATTGCGCTGACGGCATTGTTCGCGGGCAATGGCTGGATTGGCCGCTATCTGGAGCCGCTAGGCATC
AAGGTGGCCTTCACCCCGCTGGGCGTGGTGGTGGCGCTGACTTTCATTGGCCTGCCGTTCGTCGTACGCACGGTGCAGCC
GGTGCTCGAAGACGTCGAGCAGGAGCTCGAAGAAGCCGCGGCCAGCCTCGGTGCCACGCGTCTGCAGACCTTTATCCGCG
TGATCCTGCCCGCCATCCTGCCCGCGCTGCTGACTGGCTTTGCGCTGTCGTTTGCACGGGCCACCGGCGAGTACGGCTCA
GTGGTGTTCATCTCCGGCAACATGCCAATGGTCTCGGAAATTGCGCCGCTGATGATCTATTCGAAGCTCGAGCAATACGA
CTACGCCGGCGCCACCGCCGTGGCCGTGGTGATGCTGGTGATTTCCTTCGGGCTGCTGCTCGTGATCAACCTGCTGCAGG
CCTGGACGCGCCGCCACCAGTCGGGCGCGCATAGCGGCCTGGCACTCGAAGCGCCCGCCACGGACAAGGAGCACTGA

Upstream 100 bases:

>100_bases
AAGGCGAGATCTGCAGAATTTGTCTCGTAGAGCCGGTGCTTTATGCGCCGGTTCATAAGGCCCCGCGCCACGTCGCGGGG
CCGATCACCAACGAGACGAC

Downstream 100 bases:

>100_bases
CATGGCCGGCGCAGTTTCGGGCCGCCTTGGGCGGCAGGGTGGGGCCCACCACAAGTTCGACGCCACCGGCGAAGCCGTAT
GGGTGCGCTTCACGCTGATC

Product: sulphate transport system permease protein 2:Sulfate ABC transporter, permease protein CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MPPTISLADTPPPAAPDSRASRSAPGRNSRQRFTVLPGFGLSLGFTIFYLTLIVLVPLSATFLKTFTMTWEAFWTTVTAP
RVVASYQLSFGASLIAAIINTVFGLVVAWVLVRYRFPGKRLIDALVDLPFALPTAVAGIALTALFAGNGWIGRYLEPLGI
KVAFTPLGVVVALTFIGLPFVVRTVQPVLEDVEQELEEAAASLGATRLQTFIRVILPAILPALLTGFALSFARATGEYGS
VVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAVAVVMLVISFGLLLVINLLQAWTRRHQSGAHSGLALEAPATDKEH

Sequences:

>Translated_318_residues
MPPTISLADTPPPAAPDSRASRSAPGRNSRQRFTVLPGFGLSLGFTIFYLTLIVLVPLSATFLKTFTMTWEAFWTTVTAP
RVVASYQLSFGASLIAAIINTVFGLVVAWVLVRYRFPGKRLIDALVDLPFALPTAVAGIALTALFAGNGWIGRYLEPLGI
KVAFTPLGVVVALTFIGLPFVVRTVQPVLEDVEQELEEAAASLGATRLQTFIRVILPAILPALLTGFALSFARATGEYGS
VVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAVAVVMLVISFGLLLVINLLQAWTRRHQSGAHSGLALEAPATDKEH
>Mature_317_residues
PPTISLADTPPPAAPDSRASRSAPGRNSRQRFTVLPGFGLSLGFTIFYLTLIVLVPLSATFLKTFTMTWEAFWTTVTAPR
VVASYQLSFGASLIAAIINTVFGLVVAWVLVRYRFPGKRLIDALVDLPFALPTAVAGIALTALFAGNGWIGRYLEPLGIK
VAFTPLGVVVALTFIGLPFVVRTVQPVLEDVEQELEEAAASLGATRLQTFIRVILPAILPALLTGFALSFARATGEYGSV
VFISGNMPMVSEIAPLMIYSKLEQYDYAGATAVAVVMLVISFGLLLVINLLQAWTRRHQSGAHSGLALEAPATDKEH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=260, Percent_Identity=52.3076923076923, Blast_Score=254, Evalue=5e-69,
Organism=Escherichia coli, GI87082099, Length=276, Percent_Identity=31.8840579710145, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786980, Length=212, Percent_Identity=34.9056603773585, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787369, Length=213, Percent_Identity=28.6384976525822, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787368, Length=246, Percent_Identity=28.8617886178862, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786254, Length=254, Percent_Identity=27.1653543307087, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787080, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34122; Mature: 33990

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPTISLADTPPPAAPDSRASRSAPGRNSRQRFTVLPGFGLSLGFTIFYLTLIVLVPLSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
TFLKTFTMTWEAFWTTVTAPRVVASYQLSFGASLIAAIINTVFGLVVAWVLVRYRFPGKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LIDALVDLPFALPTAVAGIALTALFAGNGWIGRYLEPLGIKVAFTPLGVVVALTFIGLPF
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
VVRTVQPVLEDVEQELEEAAASLGATRLQTFIRVILPAILPALLTGFALSFARATGEYGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAVAVVMLVISFGLLLVINLLQAWTRRHQ
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAHSGLALEAPATDKEH
CCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
PPTISLADTPPPAAPDSRASRSAPGRNSRQRFTVLPGFGLSLGFTIFYLTLIVLVPLSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
TFLKTFTMTWEAFWTTVTAPRVVASYQLSFGASLIAAIINTVFGLVVAWVLVRYRFPGKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LIDALVDLPFALPTAVAGIALTALFAGNGWIGRYLEPLGIKVAFTPLGVVVALTFIGLPF
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
VVRTVQPVLEDVEQELEEAAASLGATRLQTFIRVILPAILPALLTGFALSFARATGEYGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAVAVVMLVISFGLLLVINLLQAWTRRHQ
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAHSGLALEAPATDKEH
CCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]