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The map label for this gene is dnaK [H]

Identifier: 73541441

GI number: 73541441

Start: 1905158

End: 1907002

Strand: Direct

Name: dnaK [H]

Synonym: Reut_A1752

Alternate gene names: 73541441

Gene position: 1905158-1907002 (Clockwise)

Preceding gene: 73541440

Following gene: 73541442

Centisome position: 50.05

GC content: 67.86

Gene sequence:

>1845_bases
ATGAGCGAGGCCCGCTATTCCATCGGCATCGACCTGGGCACCACGCACAGCGCCCTGTCCTACGTCGACCTGACCGGCAG
CGACGGCGAGAAGACCAGCCAGGGTGTCCTGGCGATCCCGCAGCTGACCGGACCGGGTGCGACGGAAGCACGCGATCTGC
TGCCCTCCTTCCTGTATCTGCCGCACGAGAGCGAGCTGGCGCCGGGCGACCTGGCCCTGCCATGGAGTGCCACGCGCGAA
TTCGCCGTGGGCGAGCTGGCTCGCAGCCGCGGTGCCGGCACCCCTATCCGGCTGGTATCGAGTGCAAAAAGCTGGCTGTG
TCACCCCGGCGTGGACCGCCGCGCCGCGATCCTGCCTAACGATGCGCCGCCTGAAGTGCCGCGCGTATCGCCGCTCGAGG
CATCGGTGCGTTATCTCACCCACCTGCGCGAAGCGTGGGACCAGGCGCATCCCGACGCGCCGTTCGCCGAGCAGGACGTG
ACGGTGACGATTCCGGCCTCGTTCGACCCGGCCGCGCGCGAACTCACCGCAGAGGCCGCCGCGGCCGCTGGCTACGCGCG
CATGACGCTGCTCGAAGAGCCGCAGGCTGCGCTCTATAGCTGGATCGAGAAAAGCGGTGGCCAGTGGCGCAAGGACGTCA
AGGTCGGCGACATCATCCTCGTCGTCGACGTTGGCGGCGGCACCACCGACCTGTCGCTGATCGCAGTCATCGAGCGTGAT
GGCAACCTGGAACTGCATCGTGTGGCCGTCGGCGAGCATATCCTGCTCGGCGGCGACAACATGGATCTGGCACTGGCCCA
CGTAGTCGCGCGCAAGCTCGCTGCGCAAGGCACGCAAGCCGACCCGTGGCAACTGCGCGCGCTGACCTATGCGTGCCGGG
CGGCCAAGGAAACGCTGCTGACTGACCCGACGGTGGATACCGTGCCGCTGGTCGTGCCCAGCCGCGGCTCCAAGCTGATC
GGCGGATCGATCCGCACGGACCTGACGCGTACGGAACTCACCCAGATCATCCTGGAAGGCTTCTTCCCGCAGGTCGACGC
TGCGGCCCGGCCGGTAAGCCGTGCGCGCGTCGGCCTGACGCAGCTTGGCCTGCCCTATGCACAGGATGCGGCAATCACGC
GGCATCTGGCAGCCTTCCTTGGCCGCCAGGTCGGTGCGCTCGCCGATCTGGAAGGGTTCCGGGGTACGCGGCCGGAAAGC
GCGACTTTCCTGCATCCGACCGCAGTACTGTTCAACGGCGGCGTATTCAAATCGGGCCTGCTCGCGGACCGTATCCAGCA
GACCCTGAACGGCTGGCTTGCCGCAGAAGGCGCGGCCCCTGCCCGGCTGCTCGGCGGTGCCGAACTGGACCTGGCCGTGG
CACGCGGCGCCGCGTACTACGGTTATGTGCGACGCGGCAAGGGCGTGCGCATCCGTGGCGGCACCGCGCGCTCGTATTAC
ATCGCCGTGGAATCGTCGATGCCCGCCGTGCCGGGGTTCGAACCGCCGATCCAGGCGCTGTGTGTGGCACCGTTTGGCAT
GGAAGAAGGCACCGAAACCGAATTGCCGCCGCAGGAACTCGGCCTTGTGGTCGGCGAACCAGTGCATTTCCGGTTCTTCG
GCTCCTCGGTGCGCCGCCAGGACCAGGTCGGTACCTTGCTCGATTTCTGGGGCCCCGAGGAACTGCATGAACTGGAGGAA
ATCCAGGCGACCCTGCCGGCCGAAGGACGCACGCCGGGCGAAGTCGTTCCGGTCAGGCTGCACGCACGCGTGACGGAAGC
GGGCACGCTAGAACTCGAGGCCGTCCGCGGCAACGACGAGCGCTGGAAGGTCGAATTCGACGTGCGCGGCGGCGCGCAGG
GTTGA

Upstream 100 bases:

>100_bases
GCAACCTCAGCCATCGCGGCTGGAAGGCCGAGGAAGTCAGGCTGCCGCGCCTGGCCGAGCGCCATGACGCCACCGTCATC
GCCCCCGCGGAGGTCGAGCT

Downstream 100 bases:

>100_bases
ACACCGTTTAGCGGATAAGCAAGGAAGCAATGAAGCGGTACACCGTCGGCATTGACCTTGGCACCAGCAATACCGTGGTG
GCCTATGCGGAGACAGGATC

Product: putative heat-shock chaperone protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MSEARYSIGIDLGTTHSALSYVDLTGSDGEKTSQGVLAIPQLTGPGATEARDLLPSFLYLPHESELAPGDLALPWSATRE
FAVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPNDAPPEVPRVSPLEASVRYLTHLREAWDQAHPDAPFAEQDV
TVTIPASFDPAARELTAEAAAAAGYARMTLLEEPQAALYSWIEKSGGQWRKDVKVGDIILVVDVGGGTTDLSLIAVIERD
GNLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRAAKETLLTDPTVDTVPLVVPSRGSKLI
GGSIRTDLTRTELTQIILEGFFPQVDAAARPVSRARVGLTQLGLPYAQDAAITRHLAAFLGRQVGALADLEGFRGTRPES
ATFLHPTAVLFNGGVFKSGLLADRIQQTLNGWLAAEGAAPARLLGGAELDLAVARGAAYYGYVRRGKGVRIRGGTARSYY
IAVESSMPAVPGFEPPIQALCVAPFGMEEGTETELPPQELGLVVGEPVHFRFFGSSVRRQDQVGTLLDFWGPEELHELEE
IQATLPAEGRTPGEVVPVRLHARVTEAGTLELEAVRGNDERWKVEFDVRGGAQG

Sequences:

>Translated_614_residues
MSEARYSIGIDLGTTHSALSYVDLTGSDGEKTSQGVLAIPQLTGPGATEARDLLPSFLYLPHESELAPGDLALPWSATRE
FAVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPNDAPPEVPRVSPLEASVRYLTHLREAWDQAHPDAPFAEQDV
TVTIPASFDPAARELTAEAAAAAGYARMTLLEEPQAALYSWIEKSGGQWRKDVKVGDIILVVDVGGGTTDLSLIAVIERD
GNLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRAAKETLLTDPTVDTVPLVVPSRGSKLI
GGSIRTDLTRTELTQIILEGFFPQVDAAARPVSRARVGLTQLGLPYAQDAAITRHLAAFLGRQVGALADLEGFRGTRPES
ATFLHPTAVLFNGGVFKSGLLADRIQQTLNGWLAAEGAAPARLLGGAELDLAVARGAAYYGYVRRGKGVRIRGGTARSYY
IAVESSMPAVPGFEPPIQALCVAPFGMEEGTETELPPQELGLVVGEPVHFRFFGSSVRRQDQVGTLLDFWGPEELHELEE
IQATLPAEGRTPGEVVPVRLHARVTEAGTLELEAVRGNDERWKVEFDVRGGAQG
>Mature_613_residues
SEARYSIGIDLGTTHSALSYVDLTGSDGEKTSQGVLAIPQLTGPGATEARDLLPSFLYLPHESELAPGDLALPWSATREF
AVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPNDAPPEVPRVSPLEASVRYLTHLREAWDQAHPDAPFAEQDVT
VTIPASFDPAARELTAEAAAAAGYARMTLLEEPQAALYSWIEKSGGQWRKDVKVGDIILVVDVGGGTTDLSLIAVIERDG
NLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRAAKETLLTDPTVDTVPLVVPSRGSKLIG
GSIRTDLTRTELTQIILEGFFPQVDAAARPVSRARVGLTQLGLPYAQDAAITRHLAAFLGRQVGALADLEGFRGTRPESA
TFLHPTAVLFNGGVFKSGLLADRIQQTLNGWLAAEGAAPARLLGGAELDLAVARGAAYYGYVRRGKGVRIRGGTARSYYI
AVESSMPAVPGFEPPIQALCVAPFGMEEGTETELPPQELGLVVGEPVHFRFFGSSVRRQDQVGTLLDFWGPEELHELEEI
QATLPAEGRTPGEVVPVRLHARVTEAGTLELEAVRGNDERWKVEFDVRGGAQG

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI34419635, Length=372, Percent_Identity=24.4623655913978, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1786196, Length=306, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788875, Length=304, Percent_Identity=28.9473684210526, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17541098, Length=209, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17534015, Length=305, Percent_Identity=26.8852459016393, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17534013, Length=305, Percent_Identity=26.8852459016393, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323004, Length=388, Percent_Identity=23.4536082474227, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319314, Length=389, Percent_Identity=23.9074550128535, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320950, Length=319, Percent_Identity=26.0188087774295, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17647515, Length=301, Percent_Identity=26.578073089701, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24663992, Length=173, Percent_Identity=31.2138728323699, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24663988, Length=173, Percent_Identity=31.2138728323699, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 65693; Mature: 65562

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00297 HSP70_1 ; PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEARYSIGIDLGTTHSALSYVDLTGSDGEKTSQGVLAIPQLTGPGATEARDLLPSFLYL
CCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCC
PHESELAPGDLALPWSATREFAVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPN
CCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEECCC
DAPPEVPRVSPLEASVRYLTHLREAWDQAHPDAPFAEQDVTVTIPASFDPAARELTAEAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
AAAGYARMTLLEEPQAALYSWIEKSGGQWRKDVKVGDIILVVDVGGGTTDLSLIAVIERD
HHCCHHEEEECCCHHHHHHHHHHHCCCCEECCCEECCEEEEEECCCCCCCEEEEEEEECC
GNLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRAAKETLL
CCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
TDPTVDTVPLVVPSRGSKLIGGSIRTDLTRTELTQIILEGFFPQVDAAARPVSRARVGLT
CCCCCCCEEEEECCCCCEEECCCHHHCHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCHH
QLGLPYAQDAAITRHLAAFLGRQVGALADLEGFRGTRPESATFLHPTAVLFNGGVFKSGL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEECCCCCCCHH
LADRIQQTLNGWLAAEGAAPARLLGGAELDLAVARGAAYYGYVRRGKGVRIRGGTARSYY
HHHHHHHHHCCEEEECCCCCHHHHCCCCEEEHHHCCCHHHHHHCCCCCEEEECCCCEEEE
IAVESSMPAVPGFEPPIQALCVAPFGMEEGTETELPPQELGLVVGEPVHFRFFGSSVRRQ
EEEECCCCCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHCCEEECCCEEEEECCCHHHHH
DQVGTLLDFWGPEELHELEEIQATLPAEGRTPGEVVPVRLHARVTEAGTLELEAVRGNDE
HHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEECCCC
RWKVEFDVRGGAQG
EEEEEEEECCCCCC
>Mature Secondary Structure 
SEARYSIGIDLGTTHSALSYVDLTGSDGEKTSQGVLAIPQLTGPGATEARDLLPSFLYL
CCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCC
PHESELAPGDLALPWSATREFAVGELARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPN
CCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEECCC
DAPPEVPRVSPLEASVRYLTHLREAWDQAHPDAPFAEQDVTVTIPASFDPAARELTAEAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
AAAGYARMTLLEEPQAALYSWIEKSGGQWRKDVKVGDIILVVDVGGGTTDLSLIAVIERD
HHCCHHEEEECCCHHHHHHHHHHHCCCCEECCCEECCEEEEEECCCCCCCEEEEEEEECC
GNLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRAAKETLL
CCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
TDPTVDTVPLVVPSRGSKLIGGSIRTDLTRTELTQIILEGFFPQVDAAARPVSRARVGLT
CCCCCCCEEEEECCCCCEEECCCHHHCHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCHH
QLGLPYAQDAAITRHLAAFLGRQVGALADLEGFRGTRPESATFLHPTAVLFNGGVFKSGL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEECCCCCCCHH
LADRIQQTLNGWLAAEGAAPARLLGGAELDLAVARGAAYYGYVRRGKGVRIRGGTARSYY
HHHHHHHHHCCEEEECCCCCHHHHCCCCEEEHHHCCCHHHHHHCCCCCEEEECCCCEEEE
IAVESSMPAVPGFEPPIQALCVAPFGMEEGTETELPPQELGLVVGEPVHFRFFGSSVRRQ
EEEECCCCCCCCCCCCHHHEEECCCCCCCCCCCCCCHHHCCEEECCCEEEEECCCHHHHH
DQVGTLLDFWGPEELHELEEIQATLPAEGRTPGEVVPVRLHARVTEAGTLELEAVRGNDE
HHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEECCCC
RWKVEFDVRGGAQG
EEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]