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The map label for this gene is rpoD [H]

Identifier: 73541439

GI number: 73541439

Start: 1902325

End: 1904286

Strand: Direct

Name: rpoD [H]

Synonym: Reut_A1750

Alternate gene names: 73541439

Gene position: 1902325-1904286 (Clockwise)

Preceding gene: 73541431

Following gene: 73541440

Centisome position: 49.98

GC content: 64.42

Gene sequence:

>1962_bases
ATGACGAGCGGTCCACAACGAACGGCTGCGAAAGCCCGCGAAGCAGGTATTGTCAAGGGCCTCGGCGGCTCAACGGAATC
CATGTCGCCGGATGAAGCCAATGCGCGCACCCAGCAACTGCGTGCGCTGATCCAGCTGGGCAGGCAACGTGGGTATCTGA
CCCACGCGGATATCAGCGACCATCTGCAAGAGAACTTCACCGACACGGCGGCGATGGAAAGCATCGTCACGACGTTCGCC
GAAATGGGCGTGAAGATCTTCGAGCAGACGCCCGATGCCGAGACGCTGCTGCTTAGCGACAACGCAGTAGTCGCTTCCGA
CGACCAGGCCGATGAAGAAGCCGAAGTGGCGCTTTCCACCGTGGACTCGGAGTTCGGCCGCACGACCGATCCGGTGCGCA
TGTACATGCGCGAGATGAGCTCCGCCACGCTGCTGACGCGCAAGGAAGAAGTCGAGCTCGCCAAGCGGATCGAAGATGGC
CTGAACAATATGGTGCACGCCATTTCGGCCTGCCCGTCCACGATTGCCACCATCCTCGAACTGTCGGGCCGCGTGGCCAG
CGCCGAGATGGGCATCGACGATCTGGTCGACGGCCTGACCGACGAAAGCGGCGCTGATGCTCCCGTGACCGCCAGCGCTG
ACGATAGCGACGACGACAGCGCCGACACCGCGGATGAAGACGACAGCAACGAGGACGACGACGATGCCGAAGAAGGCTCG
ACGCAGCAGAGCGCTGAAGCCGATCTCGCCAAGCTGCGCGACGAATGCCTGAAGCGCTTTGCGCGCGTGTCCGAGCAGTT
CGAGCTGATGCGGCAGGAATACGCCGCCAACGGCAGCGGATCGGCAGGGTTCGTGACCGCCCGCGACACGGTGCGCGACG
AGCTGCGTACGATCCGCTTCACCGCAAAGACCATTGAACGCCTGTGCGCCGATGTCCAGTCCATGGTCGACGATGTACGC
GTGGTCGAGCGCCAGGTGGTCCAGCTGCTGGTCGACCGTTGCGGCATGGACCGCGAGGACGTCATCGCACGCTTCCCTGG
CAATGAAACCAACCTGAGCTGGGGCCGCGACCTTGCCGCCGCATCGCGCCCGTACAGCGCCGCCGTCGCGCGCGCGCTGC
CGGATCTCGAAGCGCATCAGCAAAAGCTGATCGACATCCAGGCCCGCGCGGCGCTCCCGCTGGCCGAGCTGAAGGCGGTG
AACCGCAAGATGCTGGCCGCCGAGCGCCAGATGCGCCAGGCCAAGCACGAGATGACCCAGGCCAACCTGCGCCTGGTGAT
CTCGATTGCGAAGAAGTACACGAACCGCGGCCTGCAGTTCCTGGATCTGATCCAGGAAGGCAATATCGGCCTGATGAAGG
CCGTGGACAAGTTCGAGTACCGCCGCGGCTGGAAATTCTCGACGTACGCGACGTGGTGGGTGCGCCAGGCCGTGACGCGC
GCCATTGCAGACCAGGCCCGCACGATCCGCGTCCCGGTCCACATGATCGAGCAGATCAACAAGCTCAACCGCCTGTCGCG
CGAGCTCATGCAGCAGACCGGCAAGGAGCCGGATCCGGCCGTACTGGCCGAGCGCATGGACATGCCCGAAGAAAAGGTCC
GCGCGATCATGAAGATCGCCCGCGAACCGGTCTCGATGGAAACGCCCGTGGGCGAAGACGGCGACACGAGCCTCGGCGAC
ATGATTGCCGACTCGGACACCGCGACGCCGGCCGACGCCGCCCTCCAGGCGAGCCTGCGCGAAGTCGTGCGTGAAATGCT
CGACGAGCTCACGCCGCGTGAAGCCAAGGTGCTGCGCATGCGCTTCGGCATCGATATGGCCACCGACTACACGTTGGAAG
AAGTCGGCAAGCAGTTCGACGTGACGCGCGAGCGTATCCGCCAGATCGAAAGCAAGGCGATGCGCAAGCTGCGCCACCCG
AGCCGCGCCGATCAGCTCATCACCTATCTGCGCGACGCGTAA

Upstream 100 bases:

>100_bases
CCGAAGCCTTGACAGCGGGCCAGAAAACGCACGCAATGAGTGCGCGCTGCAATCAAAACGCGGTGCAATCAAAACGCGAT
CACGAAGGAAACAGCAGTAG

Downstream 100 bases:

>100_bases
TCGGCATGGCGGGGCATCCTGCACCCGCAGGATGCTTTCGCTTACCATGACAACGACTGCCGGCGGCCCTTGGGCTGCCG
GTTTTCGCATGGATGGCTGC

Product: sigma-70 region 1.2

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MTSGPQRTAAKAREAGIVKGLGGSTESMSPDEANARTQQLRALIQLGRQRGYLTHADISDHLQENFTDTAAMESIVTTFA
EMGVKIFEQTPDAETLLLSDNAVVASDDQADEEAEVALSTVDSEFGRTTDPVRMYMREMSSATLLTRKEEVELAKRIEDG
LNNMVHAISACPSTIATILELSGRVASAEMGIDDLVDGLTDESGADAPVTASADDSDDDSADTADEDDSNEDDDDAEEGS
TQQSAEADLAKLRDECLKRFARVSEQFELMRQEYAANGSGSAGFVTARDTVRDELRTIRFTAKTIERLCADVQSMVDDVR
VVERQVVQLLVDRCGMDREDVIARFPGNETNLSWGRDLAAASRPYSAAVARALPDLEAHQQKLIDIQARAALPLAELKAV
NRKMLAAERQMRQAKHEMTQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGWKFSTYATWWVRQAVTR
AIADQARTIRVPVHMIEQINKLNRLSRELMQQTGKEPDPAVLAERMDMPEEKVRAIMKIAREPVSMETPVGEDGDTSLGD
MIADSDTATPADAALQASLREVVREMLDELTPREAKVLRMRFGIDMATDYTLEEVGKQFDVTRERIRQIESKAMRKLRHP
SRADQLITYLRDA

Sequences:

>Translated_653_residues
MTSGPQRTAAKAREAGIVKGLGGSTESMSPDEANARTQQLRALIQLGRQRGYLTHADISDHLQENFTDTAAMESIVTTFA
EMGVKIFEQTPDAETLLLSDNAVVASDDQADEEAEVALSTVDSEFGRTTDPVRMYMREMSSATLLTRKEEVELAKRIEDG
LNNMVHAISACPSTIATILELSGRVASAEMGIDDLVDGLTDESGADAPVTASADDSDDDSADTADEDDSNEDDDDAEEGS
TQQSAEADLAKLRDECLKRFARVSEQFELMRQEYAANGSGSAGFVTARDTVRDELRTIRFTAKTIERLCADVQSMVDDVR
VVERQVVQLLVDRCGMDREDVIARFPGNETNLSWGRDLAAASRPYSAAVARALPDLEAHQQKLIDIQARAALPLAELKAV
NRKMLAAERQMRQAKHEMTQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGWKFSTYATWWVRQAVTR
AIADQARTIRVPVHMIEQINKLNRLSRELMQQTGKEPDPAVLAERMDMPEEKVRAIMKIAREPVSMETPVGEDGDTSLGD
MIADSDTATPADAALQASLREVVREMLDELTPREAKVLRMRFGIDMATDYTLEEVGKQFDVTRERIRQIESKAMRKLRHP
SRADQLITYLRDA
>Mature_652_residues
TSGPQRTAAKAREAGIVKGLGGSTESMSPDEANARTQQLRALIQLGRQRGYLTHADISDHLQENFTDTAAMESIVTTFAE
MGVKIFEQTPDAETLLLSDNAVVASDDQADEEAEVALSTVDSEFGRTTDPVRMYMREMSSATLLTRKEEVELAKRIEDGL
NNMVHAISACPSTIATILELSGRVASAEMGIDDLVDGLTDESGADAPVTASADDSDDDSADTADEDDSNEDDDDAEEGST
QQSAEADLAKLRDECLKRFARVSEQFELMRQEYAANGSGSAGFVTARDTVRDELRTIRFTAKTIERLCADVQSMVDDVRV
VERQVVQLLVDRCGMDREDVIARFPGNETNLSWGRDLAAASRPYSAAVARALPDLEAHQQKLIDIQARAALPLAELKAVN
RKMLAAERQMRQAKHEMTQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGWKFSTYATWWVRQAVTRA
IADQARTIRVPVHMIEQINKLNRLSRELMQQTGKEPDPAVLAERMDMPEEKVRAIMKIAREPVSMETPVGEDGDTSLGDM
IADSDTATPADAALQASLREVVREMLDELTPREAKVLRMRFGIDMATDYTLEEVGKQFDVTRERIRQIESKAMRKLRHPS
RADQLITYLRDA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=619, Percent_Identity=48.7883683360258, Blast_Score=573, Evalue=1e-164,
Organism=Escherichia coli, GI1789098, Length=225, Percent_Identity=41.7777777777778, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=27.5720164609054, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 72545; Mature: 72414

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGPQRTAAKAREAGIVKGLGGSTESMSPDEANARTQQLRALIQLGRQRGYLTHADISD
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHH
HLQENFTDTAAMESIVTTFAEMGVKIFEQTPDAETLLLSDNAVVASDDQADEEAEVALST
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCCHHHHHHHHH
VDSEFGRTTDPVRMYMREMSSATLLTRKEEVELAKRIEDGLNNMVHAISACPSTIATILE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGRVASAEMGIDDLVDGLTDESGADAPVTASADDSDDDSADTADEDDSNEDDDDAEEGS
HCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
TQQSAEADLAKLRDECLKRFARVSEQFELMRQEYAANGSGSAGFVTARDTVRDELRTIRF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHH
TAKTIERLCADVQSMVDDVRVVERQVVQLLVDRCGMDREDVIARFPGNETNLSWGRDLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCCCHHHH
ASRPYSAAVARALPDLEAHQQKLIDIQARAALPLAELKAVNRKMLAAERQMRQAKHEMTQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGWKFSTYATWWVRQAVTR
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AIADQARTIRVPVHMIEQINKLNRLSRELMQQTGKEPDPAVLAERMDMPEEKVRAIMKIA
HHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHH
REPVSMETPVGEDGDTSLGDMIADSDTATPADAALQASLREVVREMLDELTPREAKVLRM
HCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RFGIDMATDYTLEEVGKQFDVTRERIRQIESKAMRKLRHPSRADQLITYLRDA
HHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TSGPQRTAAKAREAGIVKGLGGSTESMSPDEANARTQQLRALIQLGRQRGYLTHADISD
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHH
HLQENFTDTAAMESIVTTFAEMGVKIFEQTPDAETLLLSDNAVVASDDQADEEAEVALST
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCCHHHHHHHHH
VDSEFGRTTDPVRMYMREMSSATLLTRKEEVELAKRIEDGLNNMVHAISACPSTIATILE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGRVASAEMGIDDLVDGLTDESGADAPVTASADDSDDDSADTADEDDSNEDDDDAEEGS
HCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
TQQSAEADLAKLRDECLKRFARVSEQFELMRQEYAANGSGSAGFVTARDTVRDELRTIRF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHH
TAKTIERLCADVQSMVDDVRVVERQVVQLLVDRCGMDREDVIARFPGNETNLSWGRDLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCCCHHHH
ASRPYSAAVARALPDLEAHQQKLIDIQARAALPLAELKAVNRKMLAAERQMRQAKHEMTQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGWKFSTYATWWVRQAVTR
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AIADQARTIRVPVHMIEQINKLNRLSRELMQQTGKEPDPAVLAERMDMPEEKVRAIMKIA
HHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHH
REPVSMETPVGEDGDTSLGDMIADSDTATPADAALQASLREVVREMLDELTPREAKVLRM
HCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RFGIDMATDYTLEEVGKQFDVTRERIRQIESKAMRKLRHPSRADQLITYLRDA
HHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA