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The map label for this gene is hyi [C]

Identifier: 73541117

GI number: 73541117

Start: 1539558

End: 1540340

Strand: Reverse

Name: hyi [C]

Synonym: Reut_A1423

Alternate gene names: 73541117

Gene position: 1540340-1539558 (Counterclockwise)

Preceding gene: 73541118

Following gene: 73541116

Centisome position: 40.47

GC content: 67.18

Gene sequence:

>783_bases
ATGCCGCGTTTCGCCGCCAACCTTTCGATGATGTACACGGAGCACGACTTCCTGGACCGCTTCGCCGCTGCCGCGGCGGA
CGGCTTCCAGGCCGTCGAGTACCTGTTCCCGTACGAGTACCCGGCCGCCGAACTGCGTGCGCGACTCGACGCCAGCGGCC
TCGTGCAGGCGCTGTTCAATGCGCCGCCGGGCGACTGGGCCGCAGGCGAGCGCGGCATTGCCGCCCTGCCCGGCCGCGAA
GCCGAGTTCCGCGATGCCTTCGGCCGCGCGCTCGAATACGCCGGCGTGATCGGCAATGACCGTGTCCACGTGATGGCCGG
CCTGATTCCCGCCGATACCGACCTCGCGCGCTGTCGTGCCACCTATCTGGAAAACCTGGCCTACGCCGCGAAGGCCGCTG
CCGCCCAAGGCATCACCGTGCTGATCGAGCCGATCAACACACGCGACATGCCCGGCTATTTCCTGAACCGCCAGGACGAC
GGCCAGGCCATCTGCAAGGAAGTCGGCGCCGCGAACCTGAAGGTGCAGTTCGACTGCTACCACTGCCAGATCGTCGAAGG
CGATGTCACGAAGAAACTGCAGCGCGACTTCGGCGGCATCGGCCATATCCAGATCGCCGGCGTGCCGGAGCGCCACGAGC
CGGACATCGGCGAACTGAACTACCCCTACCTGTTCGATGCGATCGACAGCCTCGGCTACCAGGGCTGGATCGGCTGCGAA
TACCGGCCGCGCGCCGGCACCTCGGCCGGCCTGGGCTGGATCAAGCCGTACCTGAAGCGCTGA

Upstream 100 bases:

>100_bases
CGCGATCCTGGTCATTGGCGCGGTGCTCGTGTGGCTGACGCCGGCCAAACTGGTCAACCGCTGATCCGCCGCTTTAAAGA
CTCACAACTGGAATCCAACC

Downstream 100 bases:

>100_bases
ACCCTCTGAGAAGCATTGCCATGAACGTACTGATTACCGGCGGCGCCGGCTTCCTCGGCCTGCAACTGGCGCGCCTGCTG
TTGCAGCGCGGCTCCCTGAA

Product: hydroxypyruvate isomerase

Products: 2-hydroxy-3-oxopropanoate

Alternate protein names: ORF4 [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MPRFAANLSMMYTEHDFLDRFAAAAADGFQAVEYLFPYEYPAAELRARLDASGLVQALFNAPPGDWAAGERGIAALPGRE
AEFRDAFGRALEYAGVIGNDRVHVMAGLIPADTDLARCRATYLENLAYAAKAAAAQGITVLIEPINTRDMPGYFLNRQDD
GQAICKEVGAANLKVQFDCYHCQIVEGDVTKKLQRDFGGIGHIQIAGVPERHEPDIGELNYPYLFDAIDSLGYQGWIGCE
YRPRAGTSAGLGWIKPYLKR

Sequences:

>Translated_260_residues
MPRFAANLSMMYTEHDFLDRFAAAAADGFQAVEYLFPYEYPAAELRARLDASGLVQALFNAPPGDWAAGERGIAALPGRE
AEFRDAFGRALEYAGVIGNDRVHVMAGLIPADTDLARCRATYLENLAYAAKAAAAQGITVLIEPINTRDMPGYFLNRQDD
GQAICKEVGAANLKVQFDCYHCQIVEGDVTKKLQRDFGGIGHIQIAGVPERHEPDIGELNYPYLFDAIDSLGYQGWIGCE
YRPRAGTSAGLGWIKPYLKR
>Mature_259_residues
PRFAANLSMMYTEHDFLDRFAAAAADGFQAVEYLFPYEYPAAELRARLDASGLVQALFNAPPGDWAAGERGIAALPGREA
EFRDAFGRALEYAGVIGNDRVHVMAGLIPADTDLARCRATYLENLAYAAKAAAAQGITVLIEPINTRDMPGYFLNRQDDG
QAICKEVGAANLKVQFDCYHCQIVEGDVTKKLQRDFGGIGHIQIAGVPERHEPDIGELNYPYLFDAIDSLGYQGWIGCEY
RPRAGTSAGLGWIKPYLKR

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=265, Percent_Identity=42.6415094339623, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI300068964, Length=243, Percent_Identity=41.9753086419753, Blast_Score=184, Evalue=8e-47,
Organism=Escherichia coli, GI1786718, Length=257, Percent_Identity=47.8599221789883, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789095, Length=257, Percent_Identity=49.0272373540856, Blast_Score=257, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17552022, Length=254, Percent_Identity=40.1574803149606, Blast_Score=190, Evalue=7e-49,
Organism=Drosophila melanogaster, GI17530883, Length=256, Percent_Identity=36.71875, Blast_Score=170, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 5.3.1.22

Molecular weight: Translated: 28545; Mature: 28413

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFAANLSMMYTEHDFLDRFAAAAADGFQAVEYLFPYEYPAAELRARLDASGLVQALFN
CCCHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHC
APPGDWAAGERGIAALPGREAEFRDAFGRALEYAGVIGNDRVHVMAGLIPADTDLARCRA
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHH
TYLENLAYAAKAAAAQGITVLIEPINTRDMPGYFLNRQDDGQAICKEVGAANLKVQFDCY
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEE
HCQIVEGDVTKKLQRDFGGIGHIQIAGVPERHEPDIGELNYPYLFDAIDSLGYQGWIGCE
EEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCEECCC
YRPRAGTSAGLGWIKPYLKR
CCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
PRFAANLSMMYTEHDFLDRFAAAAADGFQAVEYLFPYEYPAAELRARLDASGLVQALFN
CCHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHC
APPGDWAAGERGIAALPGREAEFRDAFGRALEYAGVIGNDRVHVMAGLIPADTDLARCRA
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHH
TYLENLAYAAKAAAAQGITVLIEPINTRDMPGYFLNRQDDGQAICKEVGAANLKVQFDCY
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEE
HCQIVEGDVTKKLQRDFGGIGHIQIAGVPERHEPDIGELNYPYLFDAIDSLGYQGWIGCE
EEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCEECCC
YRPRAGTSAGLGWIKPYLKR
CCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: hydroxypyruvate

Specific reaction: hydroxypyruvate = 2-hydroxy-3-oxopropanoate; Other Name(s): hydroxypyruvate ketol-isomerase

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7851418 [H]