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The map label for this gene is dhaT [H]

Identifier: 73541112

GI number: 73541112

Start: 1534381

End: 1535529

Strand: Reverse

Name: dhaT [H]

Synonym: Reut_A1418

Alternate gene names: 73541112

Gene position: 1535529-1534381 (Counterclockwise)

Preceding gene: 73541116

Following gene: 73541111

Centisome position: 40.34

GC content: 67.97

Gene sequence:

>1149_bases
ATGGCCTTTATCTACTATCTGACGCACATTCATCTCGACTTTGGCGCCGTGAGCCTGCTAAAGGCCGAATGTGAACGCAT
CGGCATCCGCCGCCCGCTGCTAGTGACGGACAAGGGCGTGGTCGCAGCCGGCGTGGCACAGCGAGCGATCGACGCGATGC
AGGGCCTGCCGGTGGCGGTGTTTGACGAGACGCCGTCGAATCCGACCGAAGCCATGGTGCGCAAGGCCGCCGAGCAGTAC
CGTGACGCAGGCTGCGACGGGCTGGTGGCGGTCGGCGGCGGTTCGTCGATCGATCTCGCCAAGGGCATCGCGATCATCGC
CACGCACCCGGGCGAACTGACGACCTACGCCACCATCGAAGGCGGCAGCACGAAGATCACCGAGCGCGCCGCGCCGCTGA
TCGCCGTGCCGACCACATCGGGCACCGGCAGCGAAGTGGCGCGCGGCGCGATCATCATCCTCGAAGACGGGCGCAAGCTC
GGCTTCCATTCGTGGCACCTGCTGCCGAAATCGGCCATCTGCGACCCGGAACTCACGCTGGGGCTGCCTGCGGGCCTGAC
CGCCGCGACCGGCATGGACGCCATTGCCCATTGCGTCGAGACCTTCCTGGCACCTGCCTTCAACCCGCCCGCCGACGGCA
TTGCGCTGGATGGCCTGGAGCGAGGCTGGGCCCACATCGAACGCGCCACGCGCGACGGCAAGGACCGCGACGCGCGGCTC
AACATGATGAGCGCGTCGATGCAGGGCGCCATGGCGTTCCAGAAGGGCCTTGGCTGCGTGCATTCGCTGTCGCACCCGCT
CGGCGGACTGAAGATCGACGGCAAGACCGGCCTGCACCACGGCACCCTCAACGCGGTGGTCATGCCCGCGGTGCTGCGCT
TCAACGCCGACGCGCCCACCGTGGTGCGCGACGACCGCTACGCGCGCCTGCGCCGCGCCATGCACCTGCCCGAAGGCGCC
GACGTCGCCCAGGCCGTGCACGACATGACGGCCCGGCTGGGGCTGCCGACCGGGCTGCGCCAGATGGGCGTCACCGAAGA
CATGTTCGACAAGGTGATTGCGGGCGCCCTGCTCGACCACTGCCACAAGACCAATCCAAAAGAGGCCAGCGCGGCGGATT
ATCGGCGTATGCTTGGAGCGTCCATGTAG

Upstream 100 bases:

>100_bases
AGTGGGCGTTTTTGGGCGATGATCGCCCTAGTTCATCCGCTTGGCCAGTTTTTCTCAGAATGCAAAATTCGCGGCAGATC
GCCGCAGGAGACAGCGAACC

Downstream 100 bases:

>100_bases
GCCTGCCTGGCCCTGGCCGGCCCGGGACATTCATCCACTGCGGGAGACAGCGAATGACAGGTGAACACTTGCAGGTTGTG
CATGGAGACATCACCCGGAT

Product: Iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: 1,3-propanediol oxidoreductase; 3-hydroxypropionaldehyde reductase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MAFIYYLTHIHLDFGAVSLLKAECERIGIRRPLLVTDKGVVAAGVAQRAIDAMQGLPVAVFDETPSNPTEAMVRKAAEQY
RDAGCDGLVAVGGGSSIDLAKGIAIIATHPGELTTYATIEGGSTKITERAAPLIAVPTTSGTGSEVARGAIIILEDGRKL
GFHSWHLLPKSAICDPELTLGLPAGLTAATGMDAIAHCVETFLAPAFNPPADGIALDGLERGWAHIERATRDGKDRDARL
NMMSASMQGAMAFQKGLGCVHSLSHPLGGLKIDGKTGLHHGTLNAVVMPAVLRFNADAPTVVRDDRYARLRRAMHLPEGA
DVAQAVHDMTARLGLPTGLRQMGVTEDMFDKVIAGALLDHCHKTNPKEASAADYRRMLGASM

Sequences:

>Translated_382_residues
MAFIYYLTHIHLDFGAVSLLKAECERIGIRRPLLVTDKGVVAAGVAQRAIDAMQGLPVAVFDETPSNPTEAMVRKAAEQY
RDAGCDGLVAVGGGSSIDLAKGIAIIATHPGELTTYATIEGGSTKITERAAPLIAVPTTSGTGSEVARGAIIILEDGRKL
GFHSWHLLPKSAICDPELTLGLPAGLTAATGMDAIAHCVETFLAPAFNPPADGIALDGLERGWAHIERATRDGKDRDARL
NMMSASMQGAMAFQKGLGCVHSLSHPLGGLKIDGKTGLHHGTLNAVVMPAVLRFNADAPTVVRDDRYARLRRAMHLPEGA
DVAQAVHDMTARLGLPTGLRQMGVTEDMFDKVIAGALLDHCHKTNPKEASAADYRRMLGASM
>Mature_381_residues
AFIYYLTHIHLDFGAVSLLKAECERIGIRRPLLVTDKGVVAAGVAQRAIDAMQGLPVAVFDETPSNPTEAMVRKAAEQYR
DAGCDGLVAVGGGSSIDLAKGIAIIATHPGELTTYATIEGGSTKITERAAPLIAVPTTSGTGSEVARGAIIILEDGRKLG
FHSWHLLPKSAICDPELTLGLPAGLTAATGMDAIAHCVETFLAPAFNPPADGIALDGLERGWAHIERATRDGKDRDARLN
MMSASMQGAMAFQKGLGCVHSLSHPLGGLKIDGKTGLHHGTLNAVVMPAVLRFNADAPTVVRDDRYARLRRAMHLPEGAD
VAQAVHDMTARLGLPTGLRQMGVTEDMFDKVIAGALLDHCHKTNPKEASAADYRRMLGASM

Specific function: Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=403, Percent_Identity=28.5359801488834, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI48994951, Length=372, Percent_Identity=33.3333333333333, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789163, Length=371, Percent_Identity=33.4231805929919, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI87082107, Length=365, Percent_Identity=32.0547945205479, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787493, Length=408, Percent_Identity=29.4117647058824, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789386, Length=355, Percent_Identity=29.8591549295775, Blast_Score=111, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17537053, Length=273, Percent_Identity=30.03663003663, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321181, Length=341, Percent_Identity=34.8973607038123, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24657991, Length=426, Percent_Identity=29.1079812206573, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.202 [H]

Molecular weight: Translated: 40362; Mature: 40231

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFIYYLTHIHLDFGAVSLLKAECERIGIRRPLLVTDKGVVAAGVAQRAIDAMQGLPVAV
CEEEEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEE
FDETPSNPTEAMVRKAAEQYRDAGCDGLVAVGGGSSIDLAKGIAIIATHPGELTTYATIE
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCEEEEEECCCCEEEEEEEC
GGSTKITERAAPLIAVPTTSGTGSEVARGAIIILEDGRKLGFHSWHLLPKSAICDPELTL
CCCCEEHHHCCCEEEEECCCCCCHHHHCCEEEEEECCCCCCCCCEECCCCCCCCCCCCEE
GLPAGLTAATGMDAIAHCVETFLAPAFNPPADGIALDGLERGWAHIERATRDGKDRDARL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCHHHH
NMMSASMQGAMAFQKGLGCVHSLSHPLGGLKIDGKTGLHHGTLNAVVMPAVLRFNADAPT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCC
VVRDDRYARLRRAMHLPEGADVAQAVHDMTARLGLPTGLRQMGVTEDMFDKVIAGALLDH
EECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHH
CHKTNPKEASAADYRRMLGASM
HHCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AFIYYLTHIHLDFGAVSLLKAECERIGIRRPLLVTDKGVVAAGVAQRAIDAMQGLPVAV
EEEEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEE
FDETPSNPTEAMVRKAAEQYRDAGCDGLVAVGGGSSIDLAKGIAIIATHPGELTTYATIE
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCEEEEEECCCCEEEEEEEC
GGSTKITERAAPLIAVPTTSGTGSEVARGAIIILEDGRKLGFHSWHLLPKSAICDPELTL
CCCCEEHHHCCCEEEEECCCCCCHHHHCCEEEEEECCCCCCCCCEECCCCCCCCCCCCEE
GLPAGLTAATGMDAIAHCVETFLAPAFNPPADGIALDGLERGWAHIERATRDGKDRDARL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCHHHH
NMMSASMQGAMAFQKGLGCVHSLSHPLGGLKIDGKTGLHHGTLNAVVMPAVLRFNADAPT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCC
VVRDDRYARLRRAMHLPEGADVAQAVHDMTARLGLPTGLRQMGVTEDMFDKVIAGALLDH
EECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHH
CHKTNPKEASAADYRRMLGASM
HHCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7721705 [H]